Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,397,727:1 | +C | 100% | coding (316/1848 nt) | mutL → | methyl‑directed mismatch repair protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,397,727 | 1 | . | C | 98.3% | 2304.7 / 23.1 | 630 | coding (316/1848 nt) | mutL | methyl‑directed mismatch repair protein |
Reads supporting (aligned to +/- strand): ref base . (7/4); new base C (309/309); total (317/313) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. |
Only 100 of 671 total aligned reads displayed.
TCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTT‑CCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTACAAC > NC_000913/4397579‑4397870 | gTGATAACGGCTGCGGTATCAAAAGAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTT‑cc < 3:1546809/149‑1 (MQ=255) gTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTT‑cc < 2:5966520/149‑1 (MQ=255) gATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTT‑cccc < 1:2913207/149‑2 (MQ=255) gTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCAc > 4:5737416/1‑149 (MQ=255) gTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCAc < 4:3295362/149‑1 (MQ=255) tCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCAATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTc > 4:142204/1‑149 (MQ=255) aaaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACtt > 1:141558/1‑146 (MQ=255) aaaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCAc < 3:6874964/149‑1 (MQ=255) aaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACg > 3:6131527/1‑149 (MQ=255) aaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCa < 4:405204/149‑1 (MQ=255) gATGAGCTGGCGCTGGCGCTGGCTCAGCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCCAAGCCATTATTAGCCTCGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGATCGGCTTC‑CCCGCCTGACGCTCACTTCACGCACCg < 4:4417706/149‑1 (MQ=255) aGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGa > 2:195650/1‑149 (MQ=255) cgctggcgctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca < 3:1174465/149‑1 (MQ=255) ggcgctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa < 1:1204284/149‑1 (MQ=255) ggcgctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa < 1:3240572/149‑1 (MQ=255) cgctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGc > 2:6437230/1‑149 (MQ=255) ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa < 3:3533208/145‑1 (MQ=255) ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa > 4:3533208/1‑145 (MQ=255) ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt < 1:2421307/149‑1 (MQ=255) tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTg > 1:1662943/1‑149 (MQ=255) ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTgg < 2:4895213/149‑1 (MQ=255) ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTgg > 3:930596/1‑149 (MQ=255) tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg > 1:872671/1‑149 (MQ=255) cGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg < 4:5322647/149‑1 (MQ=255) gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGc < 4:6112509/149‑1 (MQ=255) cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCt < 1:5727345/149‑1 (MQ=255) aTGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa > 1:4613893/1‑149 (MQ=255) accaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCg > 2:2315684/1‑149 (MQ=255) caGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa > 1:4167254/1‑149 (MQ=255) caGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCGCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa < 4:6522763/149‑1 (MQ=255) aGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCAc < 3:2463792/107‑1 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAgg < 3:5061192/149‑1 (MQ=255) tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAggg > 3:6468944/1‑149 (MQ=255) aaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcgc < 1:3602230/149‑1 (MQ=255) aaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcgc < 4:1743021/149‑1 (MQ=255) aaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcgc > 3:4359089/1‑149 (MQ=255) aaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcgc < 1:4923027/149‑1 (MQ=255) tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGa < 1:2691256/149‑1 (MQ=255) tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGa > 4:5184572/1‑149 (MQ=255) gCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata < 2:4425261/149‑1 (MQ=255) ccTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat > 4:2551586/1‑149 (MQ=255) ccTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat < 3:2804348/149‑1 (MQ=255) ccTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat > 1:4334903/1‑149 (MQ=255) tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGa > 2:7241261/1‑149 (MQ=255) ctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCGGGAAGCCTGGCAGGCCTATGCCGAAGGGCGTGATGTGaa > 4:5997164/1‑149 (MQ=255) tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAAc < 4:2069126/149‑1 (MQ=255) gaggcGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGt < 1:2060506/145‑1 (MQ=255) ggACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCGTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTg < 3:4628795/149‑1 (MQ=255) gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGa < 2:5434779/149‑1 (MQ=255) gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGa > 1:5441126/1‑149 (MQ=255) gATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa > 3:32823/1‑127 (MQ=255) gATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACgg > 3:1474283/1‑149 (MQ=255) aTCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGt < 4:3405002/149‑1 (MQ=255) aTCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGt > 2:3143582/1‑149 (MQ=255) gAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAAc > 3:5835644/1‑149 (MQ=255) gCCATTATCAGCCTGGGCTTTCGTGGTGAGGCGCTGGCGTGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTGACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACcgg > 2:772531/1‑149 (MQ=255) ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCggcggc > 3:5403853/1‑149 (MQ=255) tATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGgcggcg > 2:1536813/1‑149 (MQ=255) tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCa > 3:415895/1‑149 (MQ=255) aGCCTGGGCATTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACCTGACGGTAAAACCGGCGGCGCATc < 3:6011467/149‑1 (MQ=255) gCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATcc < 2:2633913/149‑1 (MQ=255) ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCt > 4:1932128/1‑149 (MQ=255) ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCt < 2:4657340/149‑1 (MQ=255) tGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCtgt < 1:594573/149‑1 (MQ=255) ggCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTgg < 1:6437230/149‑1 (MQ=255) gCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTggg < 3:3930558/149‑1 (MQ=255) tttCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGga > 1:5199510/1‑149 (MQ=255) tttCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGga > 1:3935357/1‑149 (MQ=255) tttCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGga < 2:3579163/149‑1 (MQ=255) tGAGGCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTg < 4:88593/149‑1 (MQ=255) ggCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGATGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAg < 4:4831740/149‑1 (MQ=255) ggCGCTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAg < 3:1853623/149‑1 (MQ=255) gcgcTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAgg > 2:877697/1‑149 (MQ=255) gcgcTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAgg > 4:2823886/1‑149 (MQ=255) gcTGGCGAGTATCAGTTAGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTg < 2:6742099/149‑1 (MQ=255) cTGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGc < 4:2197423/149‑1 (MQ=255) tGGCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCt < 3:2170241/149‑1 (MQ=255) gCGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTgg > 1:1031043/1‑149 (MQ=255) cGAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGa > 4:6982823/1‑149 (MQ=255) gAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAg > 1:1823488/1‑140 (MQ=255) gAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGAt < 3:662631/149‑1 (MQ=255) gAGTATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGAt < 2:1302607/149‑1 (MQ=255) tATCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAAc > 1:6695313/1‑48 (MQ=255) aTCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGt < 1:719690/149‑1 (MQ=255) tCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGtt > 1:3406354/1‑149 (MQ=255) tCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGtt < 3:567204/149‑1 (MQ=255) tCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGtt < 3:5197241/149‑1 (MQ=255) tCAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGtt < 2:5217367/149‑1 (MQ=255) cAGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTc > 2:331683/1‑149 (MQ=255) aGTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCt > 2:5653520/1‑149 (MQ=255) gTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTa > 4:1249301/1‑149 (MQ=255) gTTCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTa > 3:3435131/1‑149 (MQ=255) ttCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAg > 1:6901364/1‑131 (MQ=255) ttCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTac > 4:3859205/1‑149 (MQ=255) ttCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTac > 4:395766/1‑149 (MQ=255) ttCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTac > 2:2855301/1‑149 (MQ=255) ttCGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTac > 4:6627438/1‑149 (MQ=255) cGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCt > 2:40951/1‑61 (MQ=255) cGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc < 2:2459862/65‑1 (MQ=255) cGG‑TTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc > 1:2459862/1‑65 (MQ=255) | TCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGG‑TTT‑CCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGTGACGGTAAAACCGGCGGCGCATCCTGTGGGGACGACGCTGGAGGTGCTGGATCTGTTCTACAAC > NC_000913/4397579‑4397870 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |