Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	1,926,052	Δ8 bp	5.4%	coding (613‑620/657 nt)	yobB →	C‑N hydrolase family protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1926051	669 (0.850)	38 (0.050)	35/258	NT	5.4%	coding (612/657 nt)	yobB	C‑N hydrolase family protein
	?	NC_000913	1926060 =	672 (0.850)					coding (621/657 nt)	yobB	C‑N hydrolase family protein

GGCGTCGGCCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1925904‑1926051 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGA  >  NC_000913/1926060‑1926196                                                                                                                                                                                                                                                                                               GGCGTCGGCCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGA                                                                                                                                          <  1:7273216/149‑1  GCGTCGGCCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGAC                                                                                                                                         <  3:1515301/149‑1    GTCGGCCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAG                                                                                                                                       <  3:5089983/149‑1        GCCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTT                                                                                                                                   <  2:862594/149‑1         CCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTG                                                                                                                                  <  2:2046560/149‑1                CTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGC                                                                                                                           >  2:6067802/1‑149                 TTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCG                                                                                                                          >  1:4067155/1‑149                   ACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGAT                                                                                                                        <  3:4053454/149‑1                        TTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCAT                                                                                                                   <  4:6691534/149‑1                           TTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCC                                                                                                                <  2:4895792/149‑1                               ACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTA                                                                                                            >  3:6449401/1‑149                                            ATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTT                                                                                               >  3:1228194/1‑149                                                  GTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCT                                                                                         <  4:6449401/149‑1                                                  GTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCT                                                                                         >  3:5126174/1‑149                                                   TGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGCGGTTCATTATTGTTAGTCGGTCAGACAGAGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTGTCGGCCTG                                                                                        >  2:7121419/1‑149                                                     CTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGA                                                                                      <  4:2130413/149‑1                                                             AAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCA                                                                              <  3:3086861/149‑1                                                             AAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCA                                                                              >  3:6073627/1‑149                                                             AAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCA                                                                              >  2:1736492/1‑149                                                                TGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGT                                                                           <  3:793794/149‑1                                                                 GCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTT                                                                          <  1:5578765/149‑1                                                                     GTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGC                                                                      >  3:4469167/1‑149                                                                        GTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATT                                                                   <  4:6073627/149‑1                                                                          AACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAT                                                                 >  3:2266621/1‑149                                                                                     ATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAA                                                      >  3:4292392/1‑149                                                                                        GGAAGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCT                                                   >  2:5697167/1‑149                                                                                           TGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCG                                                <  4:3289581/149‑1                                                                                           TGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCG                                                >  3:846883/1‑149                                                                                                       TCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAG                                    >  1:496338/1‑149                                                                                                       TCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAG                                    >  1:6359390/1‑149                                                                                                            TCGTTCGCGCCGATCGTGGTTCATTATTCTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCTTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATC                               >  1:2611920/1‑149                                                                                                               TTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTT                            <  3:6573290/149‑1                                                                                                               TTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTT                            <  1:6067802/149‑1                                                                                                                       GATCGTGGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGC                    <  4:4469167/149‑1                                                                                                                             GGTTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGT              <  2:2212401/149‑1                                                                                                                               TTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTG                           >  1:2024013/1‑134                                                                                                                               TTCATTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTG                           <  2:2024013/134‑1                                                                                                                                 CATTATTCTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCTTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGAT          <  2:2611920/149‑1                                                                                                                                   TTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGA                                                              <  3:5467644/95‑1                                                                                                                                   TTATTGTTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGA                                                              >  4:5467644/1‑95                                                                                                                                         TTAGTCGGTCAGACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGA  <  2:1809012/149‑1                                                                                                                                                                                                                                                                                               GGCGTCGGCCCGTCGCTTACAATTTTTTTCACATCAGTACTCGATTGCCGTGCTGATGGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1925904‑1926051 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGA  >  NC_000913/1926060‑1926196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.