Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 885,311 C→T 10.7% intergenic (‑81/+5) ybjH ← / ← ybjI uncharacterized protein/5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase; pyrimidine phosphatase; riboflavin synthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913885,3110CT10.7% 2071.4 / 172.2 700intergenic (‑81/+5)ybjH/ybjIuncharacterized protein/5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase; pyrimidine phosphatase; riboflavin synthesis
Reads supporting (aligned to +/- strand):  ref base C (278/347);  new base T (38/37);  total (316/384)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

Only 100 of 707 total aligned reads displayed.

GCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTAAAGCCTGCCTG  >  NC_000913/885164‑885459
                                                                                                                                                   |                                                                                                                                                    
gcgcCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCg                                                                                                                                                     <  4:1162648/149‑1 (MQ=255)
  gcCATCGCCACACCAGTAAAGCCGATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGt                                                                                                                                                   >  2:5587283/1‑149 (MQ=255)
  gcCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCCGCCGGt                                                                                                                                                   >  1:5125669/1‑149 (MQ=255)
    cATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGttt                                                                                                                                                 >  3:235326/1‑149 (MQ=255)
    cATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGttt                                                                                                                                                 <  3:1303852/149‑1 (MQ=255)
      tCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTa                                                                                                                                               <  3:6849863/149‑1 (MQ=255)
       cGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTAt                                                                                                                                              <  1:1760478/149‑1 (MQ=255)
        gCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTAtt                                                                                                                                             <  2:4384524/149‑1 (MQ=255)
        gCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTAtt                                                                                                                                             <  1:6692153/149‑1 (MQ=255)
         ccACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTg                                                                                                                                            <  4:235326/149‑1 (MQ=255)
         ccACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTg                                                                                                                                            <  3:3681818/149‑1 (MQ=255)
          cacaCCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTgg                                                                                                                                           <  3:3461452/149‑1 (MQ=255)
           acacCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACACTTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGt                                                                                                                                          >  4:2548804/1‑149 (MQ=255)
                      gCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCt                                                                                                                               >  4:4958999/1‑149 (MQ=255)
                      ccccATTAAAAGCGCGCCCAACAATAAACAATTTTTCTTAATCATATCGCCTTAAATAATGCAGCAGGTTATTTTCCGCGAATTATGCAGCCTTCATACAGGCATTGTGTTGCCGAGCGTACTGCTGGGTTTATTGGTCAAATGGCGCt                                                                                                                               <  2:1764324/148‑1 (MQ=255)
                       cccATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCtt                                                                                                                              >  1:4232373/1‑149 (MQ=255)
                       cccATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCtt                                                                                                                              >  3:2045194/1‑149 (MQ=255)
                        ccATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTc                                                                                                                             >  3:2697986/1‑149 (MQ=255)
                        ccATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTc                                                                                                                             >  2:5066093/1‑149 (MQ=255)
                          aTTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCgt                                                                                                                           >  4:1870359/1‑149 (MQ=255)
                            tAAAAGCGCGCCCAACAGTAGACAATTTCTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCgtgt                                                                                                                         <  1:5286786/149‑1 (MQ=255)
                             aaaaGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGtt                                                                                                                        <  4:2843811/149‑1 (MQ=255)
                                aGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCCTCGTGTTTaa                                                                                                                     <  3:455454/149‑1 (MQ=255)
                                aGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTCTTATTGGTCAAATGGCGCTTCGTGTTTaa                                                                                                                     <  3:4250857/149‑1 (MQ=255)
                                 gcgcgcCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAg                                                                                                                    >  2:6346522/1‑149 (MQ=255)
                                 gcgcgcCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAg                                                                                                                    >  1:754088/1‑149 (MQ=255)
                                 gcgcgcCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAg                                                                                                                    >  2:2749677/1‑149 (MQ=255)
                                     gcCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACt                                                                                                                >  1:6335631/1‑149 (MQ=255)
                                       ccAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCaaa                                                                                                                                      >  1:4490778/1‑125 (MQ=255)
                                       ccAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACttt                                                                                                              >  1:3758151/1‑149 (MQ=255)
                                        cAACAGTAGACAATTTTTCATAATCATTTCGCCTGATATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTa                                                                                                             >  4:6735938/1‑149 (MQ=255)
                                          aCAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTAtt                                                                                                                                             >  2:1209268/1‑115 (MQ=255)
                                            aGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGa                                                                                                         <  2:6545598/149‑1 (MQ=255)
                                             gTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGAt                                                                                                        <  1:6028680/149‑1 (MQ=255)
                                               aGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATca                                                                                                      <  1:3544339/149‑1 (MQ=255)
                                                gACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATcac                                                                                                     >  4:3470539/1‑149 (MQ=255)
                                                gACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTACCGATcac                                                                                                     >  2:2076908/1‑149 (MQ=255)
                                                  cAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACAt                                                                                                   >  4:803155/1‑149 (MQ=255)
                                                   aaTTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATc                                                                                                  >  3:6382944/1‑149 (MQ=255)
                                                   aaTTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTAt                                                                                                            <  2:6244776/139‑1 (MQ=255)
                                                   aaTTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATc                                                                                                  >  3:2964446/1‑149 (MQ=255)
                                                   aaTTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATc                                                                                                  >  3:5600324/1‑149 (MQ=255)
                                                   aaTTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATc                                                                                                  <  1:2761739/149‑1 (MQ=255)
                                                        ttCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGAGCACATCCAGTa                                                                                             >  1:2588939/1‑149 (MQ=255)
                                                        ttCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTa                                                                                             <  3:704873/149‑1 (MQ=255)
                                                           aTAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCCGGGGAGCGGCCAGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGc                                                                                          >  2:5189539/1‑149 (MQ=255)
                                                            tAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGcc                                                                                         >  3:88639/1‑149 (MQ=255)
                                                             aaTCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTCtt                                                                                                                                             >  1:7046032/1‑96 (MQ=255)
                                                                 aTTTCGCCTGAAATAATGCAGCGGGTTAATTTACGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCAc                                                                                    <  4:4268747/149‑1 (MQ=255)
                                                                  tttCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACg                                                                                   >  3:6587619/1‑149 (MQ=255)
                                                                   ttCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAGATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACgg                                                                                  <  3:2477700/149‑1 (MQ=255)
                                                                     cGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGtt                                                                                <  3:3984760/149‑1 (MQ=255)
                                                                       ccTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTAt                                                                              >  4:526998/1‑149 (MQ=255)
                                                                        cTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTGCACGGTTAtt                                                                             >  4:6095268/1‑149 (MQ=255)
                                                                          gAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATTGATCACATCCAGTACGCCTTCACGGTTATTgg                                                                           >  3:4914112/1‑149 (MQ=255)
                                                                           aaaTAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGa                                                                          <  2:5808747/149‑1 (MQ=255)
                                                                            aataatGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGa                                                                          >  3:3216097/1‑148 (MQ=255)
                                                                               aatGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTaa                                                                                                                     >  4:2123184/1‑102 (MQ=255)
                                                                               aatGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCt                                                                      >  1:3996543/1‑149 (MQ=255)
                                                                                   cAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGATTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTgcgc                                                                  <  2:1482681/149‑1 (MQ=255)
                                                                                    aGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGcgcg                                                                 >  2:4152202/1‑149 (MQ=255)
                                                                                    aGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGcgcg                                                                 <  4:5513737/149‑1 (MQ=255)
                                                                                       gggTTAATTGCCGCGAATTATGCAGCCTTCATGCAGGCAGTGTGTTGCCGGGCGGGCTGCCGGTTATATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTa                                                              >  2:4907470/1‑149 (MQ=255)
                                                                                         gTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTAtt                                                            >  1:5786773/1‑149 (MQ=255)
                                                                                           tAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTAtttt                                                          <  4:569696/149‑1 (MQ=255)
                                                                                           tAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTAtttt                                                          >  4:3075913/1‑149 (MQ=255)
                                                                                              tttCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCAc                                                                                    <  4:6304432/120‑1 (MQ=255)
                                                                                                  cgcgAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTg                                                   >  2:2904033/1‑149 (MQ=255)
                                                                                                   gcgAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGc                                                  >  2:6986643/1‑149 (MQ=255)
                                                                                                   gcgAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGc                                                  <  2:387905/149‑1 (MQ=255)
                                                                                                   gcgAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGc                                                  >  2:3202771/1‑149 (MQ=255)
                                                                                                     gAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGa                                                                                                         <  1:1023712/92‑1 (MQ=255)
                                                                                                        ttATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTAt                                                                                                            >  2:742209/1‑86 (MQ=255)
                                                                                                        ttATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGa                                                                          >  3:1024603/1‑120 (MQ=255)
                                                                                                          aTGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGAcc                                           <  1:4846050/149‑1 (MQ=255)
                                                                                                            gCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACcgc                                         <  2:3522673/149‑1 (MQ=255)
                                                                                                              cgCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACCCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCgcgc                                       <  3:622064/148‑1 (MQ=255)
                                                                                                              aGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCgcgc                                       >  2:2269132/1‑149 (MQ=255)
                                                                                                                 cTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTAc                                    >  1:5409047/1‑149 (MQ=255)
                                                                                                                   tcattcaGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCg                                  <  4:1493926/149‑1 (MQ=255)
                                                                                                                        caGGCATTGTGTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCAtt                             <  3:1503427/149‑1 (MQ=255)
                                                                                                                         aGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTa                                                                               <  2:4727503/98‑1 (MQ=255)
                                                                                                                         aGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCAttt                            >  4:4383586/1‑149 (MQ=255)
                                                                                                                           gCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTc                          >  4:6508410/1‑149 (MQ=255)
                                                                                                                             aTTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGcc                                                                       <  1:1313695/102‑1 (MQ=255)
                                                                                                                             aTTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCa                        <  4:4020164/149‑1 (MQ=255)
                                                                                                                             aTTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCa                        <  2:2466385/149‑1 (MQ=255)
                                                                                                                              ttGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCAt                       <  4:1377781/149‑1 (MQ=255)
                                                                                                                               tgtgTTGCCGGGCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCAtt                      <  3:5261650/149‑1 (MQ=255)
                                                                                                                               tgtgTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCAtt                      <  2:6827860/149‑1 (MQ=255)
                                                                                                                                   ttGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCaa                  >  4:6929087/1‑149 (MQ=255)
                                                                                                                                     gCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCAt                              >  4:5136946/1‑135 (MQ=255)
                                                                                                                                     gCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCAt                              <  3:5136946/135‑1 (MQ=255)
                                                                                                                                          gCGTGCTGCTGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTaaa           <  1:1523479/149‑1 (MQ=255)
                                                                                                                                          gCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTaaa           <  1:3407829/149‑1 (MQ=255)
                                                                                                                                              gctgctGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTAAAgcct       <  1:2204101/149‑1 (MQ=255)
                                                                                                                                              gctgcCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTAAAgcct       <  1:3037870/149‑1 (MQ=255)
                                                                                                                                                tgcCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGTTCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTAAAgcctgc     <  3:5874195/149‑1 (MQ=255)
                                                                                                                                                tgcCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTAAAgcctgc     <  3:1773150/149‑1 (MQ=255)
                                                                                                                                                 gcCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTgg                                                                           <  4:3244086/78‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTGTGTTGCCGGGCGTGCTGCCGGTTTTATTGGTCAAATGGCGCTTCGTGTTTAAGAACTTTATCGATCACATCCAGTACGCCTTCACGGTTATTGGAGCCTGCGCGGTATTTTGCCGCTGCGACGACCGCGCTACCGGCATTTTCCATTGCAAAACTAAAGCCTGCCTG  >  NC_000913/885164‑885459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: