BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,312,227	A→G	16.0%	V7A (GTC→GCC)	yciA ←	acyl‑CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,312,227	0	A	G	16.0%	2329.9 / inf	989	V7A (GTC→GCC)	yciA	acyl‑CoA esterase
Reads supporting (aligned to +/- strand): ref base A (370/457); new base G (78/82); total (450/539)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-02

Only 100 of 995 total aligned reads displayed.

AGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCTTCCTGCGGCATG  >  NC_000913/1312079‑1312375

aGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGa                                                                                                                                                      <  4:2775622/149‑1 (MQ=255)

 gTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGAc                                                                                                                                                     <  2:1448337/149‑1 (MQ=255)

      tACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTAt                                                                                                                                                >  1:7268269/1‑149 (MQ=255)

      tACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTAt                                                                                                                                                <  2:5755319/149‑1 (MQ=255)

        cgcgACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTAtgt                                                                                                                                              <  3:5580965/149‑1 (MQ=255)

         gcgACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTAtgtg                                                                                                                                             >  1:4830657/1‑149 (MQ=255)

             ccGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGtgttgt                                                                                                                                         <  1:6272259/149‑1 (MQ=255)

                       tttCTTTTGCCAGAATAGCGACGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTa                                                                                                                               >  1:5797648/1‑149 (MQ=255)

                       tttCTTTTGACAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGGACTTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTTTGGACATggga                                                                                                                               >  1:437226/1‑147 (MQ=255)

                         tCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTAAGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTaaa                                                                                                                             >  4:2473088/1‑149 (MQ=255)

                           ttttGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACa                                                                                                                                    <  3:5292732/140‑1 (MQ=255)

                           ttttGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAAc                                                                                                                           >  2:4317524/1‑149 (MQ=255)

                           ttttGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCCAAAGTACGTAAAACAAGATCGCCCCGAGGGCCGTTATGTGTTGTAGACGTGGTAAAAc                                                                                                                           >  4:6407017/1‑149 (MQ=255)

                              tGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGa                                                                                                                        <  3:373105/149‑1 (MQ=255)

                              tGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGa                                                                                                                        <  1:5657153/149‑1 (MQ=255)

                               gCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATTTGTTGTAGACATGGTAAAACCGAc                                                                                                                       >  2:6089087/1‑149 (MQ=255)

                               gCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGAc                                                                                                                       >  2:1995796/1‑149 (MQ=255)

                               gCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGAc                                                                                                                       <  2:6462701/149‑1 (MQ=255)

                                ccAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACt                                                                                                                      >  2:5439505/1‑149 (MQ=255)

                                ccAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACt                                                                                                                      >  1:673545/1‑149 (MQ=255)

                                ccAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACt                                                                                                                      >  2:3490762/1‑149 (MQ=255)

                                 cAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCCCCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACtt                                                                                                                     <  1:1269116/149‑1 (MQ=255)

                                  aGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTa                                                                                                                    >  1:2285741/1‑149 (MQ=255)

                                  aGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCACCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTa                                                                                                                    >  3:4332104/1‑149 (MQ=255)

                                     aTAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAA                                                                                                                 <  2:980738‑M1/149‑1 (MQ=255)

                                     aTAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAA                                                                                                                 <  3:6387464‑M1/149‑1 (MQ=255)

                                           ccgccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAA                                                                                                           <  2:7125345‑M1/149‑1 (MQ=255)

                                           ccgccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAA                                                                                                           <  2:4541651‑M1/149‑1 (MQ=255)

                                           ccgccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAA                                                                                                                 >  3:6078849‑M1/1‑143 (MQ=255)

                                               cAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCg                                                                                                                         <  1:5161979/131‑1 (MQ=255)

                                               cAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATcac                                                                                                       <  1:3574325/149‑1 (MQ=255)

                                               cAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATcac                                                                                                       >  4:4391379/1‑149 (MQ=255)

                                                aaTATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATcaca                                                                                                      >  1:2384369/1‑149 (MQ=255)

                                                       aTTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGa                                                                                               <  4:853269/149‑1 (MQ=255)

                                                        tttGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTAt                                                                                                                                                <  1:1008305/99‑1 (MQ=255)

                                                        tttGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGAt                                                                                              <  1:6990903/149‑1 (MQ=255)

                                                         ttttgACATTAACCAACCACGAAAGATGTCACCATTGGCATTGGTATCGGCGGTCGCGCCGCAAGTACGAAAAACAAGATCGCTCCGACGGACGTTCTGCGTTGTAGACATGGTAACACCGAATTAACAGGATAATCACAGGCTCGATg                                                                                             >  2:1096216/4‑149 (MQ=255)

                                                         ttGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGtgttgt                                                                                                                                         <  1:1985378/105‑1 (MQ=255)

                                                            tgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGcta                                                                                          >  3:1567583/1‑149 (MQ=255)

                                                              aCATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGa                                                                                                                        <  4:1773414/117‑1 (MQ=255)

                                                              aCATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAgg                                                                                                    <  4:1073005/137‑1 (MQ=255)

                                                              aCATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAAcc                                                                                                                          >  2:3762743/1‑115 (MQ=255)

                                                                aTTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAt                                                                                      <  4:5298266‑M2/149‑2 (MQ=255)

                                                                aTTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAt                                                                                      >  1:4566308‑M2/1‑148 (MQ=255)

                                                                      caaccaCCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAtgatttt                                                                                <  1:5573356‑M2/149‑8 (MQ=255)

                                                                       aaccaCCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAtgattttc                                                                               >  3:6700879‑M2/1‑141 (MQ=255)

                                                                       aaccaCCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAtgattttc                                                                               <  4:677878‑M2/149‑9 (MQ=255)

                                                                       aaccaCCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAtgattttc                                                                               >  3:2425829‑M2/1‑141 (MQ=255)

                                                                          caccaAAGATGTCACCATTGGCATTGGTATCGGCAGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGAc                                                                                                                                     <  4:3570309/92‑1 (MQ=255)

                                                                           accaAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGAt                                                                                              >  4:7035337/1‑130 (MQ=255)

                                                                           accaAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTga                                                                           <  3:1238910/149‑1 (MQ=255)

                                                                            ccaAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTgag                                                                          >  2:3248949/1‑149 (MQ=255)

                                                                            ccaAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTgag                                                                          >  4:3836800/1‑149 (MQ=255)

                                                                              aaaGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTgagga                                                                        >  1:3179069/1‑149 (MQ=255)

                                                                               aaGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTgaggag                                                                       <  1:1553652/149‑1 (MQ=255)

                                                                                 gATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGaa                                                                     <  1:2733609/149‑1 (MQ=255)

                                                                                     tCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACaga                                                                 >  4:2089267/1‑149 (MQ=255)

                                                                                      cACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACagag                                                                <  2:5601745/149‑1 (MQ=255)

                                                                                      cACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACagag                                                                <  4:230376/149‑1 (MQ=255)

                                                                                       aCCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAgagg                                                               <  1:3831532/149‑1 (MQ=255)

                                                                                        ccATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTgagga                                                                        >  2:2269062/1‑139 (MQ=255)

                                                                                        ccATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAgagga                                                              >  3:3973059/1‑149 (MQ=255)

                                                                                          attggcattggTATCGGCGGGCATGGCTAAAGTACGTAAAACGAGCTCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGTTgaga                                                            <  4:3328212/149‑1 (MQ=255)

                                                                                          attggcattggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGgaga                                                            >  2:2663297/1‑149 (MQ=255)

                                                                                          attggcattggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGgaga                                                            >  2:519092/1‑149 (MQ=255)

                                                                                           ttggcattggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGAt                                                           >  3:2899758/1‑149 (MQ=255)

                                                                                               cattggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAg                                                       <  3:4436323/149‑1 (MQ=255)

                                                                                               cattggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAg                                                       >  2:5055787/1‑149 (MQ=255)

                                                                                               cattggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAg                                                       >  4:1423806/1‑149 (MQ=255)

                                                                                                attggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAtg                                                                                     >  4:487973‑M2/1‑116 (MQ=255)

                                                                                                attggTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAgc                                                      <  3:6916509/149‑1 (MQ=255)

                                                                                                        cggcggGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGccc                                              >  3:3500871/1‑149 (MQ=255)

                                                                                                          gcggGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACagag                                                                <  1:6014243/129‑1 (MQ=255)

                                                                                                          gcggGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGAc                                                                                                                       <  2:1264682/74‑1 (MQ=255)

                                                                                                            gggCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGaa                                          <  2:2384369/149‑1 (MQ=255)

                                                                                                                aTGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGAc                                      >  4:4064056/1‑149 (MQ=255)

                                                                                                                   gCTAAAGTACGTAAAACAAGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTgg                                   <  4:4642755/149‑1 (MQ=255)

                                                                                                                   gCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTAtgattttc                                                                               >  3:4814306‑M2/1‑97 (MQ=255)

                                                                                                                   gCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGAAACAGCGCCAGCCCCGAAGGACTgg                                   >  2:4738883/1‑149 (MQ=255)

                                                                                                                    cTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACGGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGc                                  >  2:6767387/1‑149 (MQ=255)

                                                                                                                        aGTACGTAAAACAAGATCTCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTc                              <  3:5799918/149‑1 (MQ=255)

                                                                                                                         gTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCATAGTAGCATCGCGCCTGtgattttccttttaagtcggt                                                                  <  3:2141012‑M2/112‑22 (MQ=255)

                                                                                                                           aCGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTgg                           <  3:3264933/149‑1 (MQ=255)

                                                                                                                             gTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAgagga                                                              >  4:3023610/1‑112 (MQ=255)

                                                                                                                             gTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGtt                         >  2:83165/1‑149 (MQ=255)

                                                                                                                             gTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGtt                         >  1:4172444/1‑149 (MQ=255)

                                                                                                                               aaaaCAAGATCGCCCTGAGGGACGTTATGtgttgt                                                                                                                                         <  4:5625614/35‑1 (MQ=255)

                                                                                                                               aaaaCAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGt                               <  2:5873788/141‑1 (MQ=255)

                                                                                                                               aaaaCAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAg                       >  3:727983/1‑149 (MQ=255)

                                                                                                                               aaaaCAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAg                       <  3:3074951/149‑1 (MQ=255)

                                                                                                                                 aaCAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGa                     >  4:6575985/1‑149 (MQ=255)

                                                                                                                                    aaGATCGCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGAttt                  >  2:4198565/1‑149 (MQ=255)

                                                                                                                                      gATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTAt                <  1:5310312/149‑1 (MQ=255)

                                                                                                                                        tCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCt              >  2:2910883/1‑149 (MQ=255)

                                                                                                                                          gCCCTGAGGGGCGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCTTc            <  3:1911847/149‑1 (MQ=255)

                                                                                                                                          gCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGaa                                                                     <  2:1741767/92‑1 (MQ=255)

                                                                                                                                          gCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGaa                                                                     >  1:1741767/1‑92 (MQ=255)

                                                                                                                                          gCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCTTc            <  4:5992779/149‑1 (MQ=255)

                                                                                                                                            ccTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCTTCCt          >  4:6184515/1‑149 (MQ=255)

                                                                                                                                                aGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCTTCCTGCgg      >  3:275952/1‑149 (MQ=255)

AGTCACTACGCGACCGTGGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTACTATGATTTTCCTGAGGAGAACAGAGGAGATACAGCGCCAGCCCCGAAGGACTGGCAGTCTGGTTAGGATTTATCTTCCTGCGGCATG  >  NC_000913/1312079‑1312375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`