| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,317,372 | C→T | 5.7% | A348A (GCG→GCA) | trpB ← | tryptophan synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,317,372 | 0 | C | T | 5.7% | 2488.1 / 81.8 | 749 | A348A (GCG→GCA) | trpB | tryptophan synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (312/394); new base T (18/25); total (330/419) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 763 total aligned reads displayed.
AGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGATG > NC_000913/1317224‑1317519 | aGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgc > 1:454050/1‑149 (MQ=255) aTTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc > 1:4306070/1‑149 (MQ=255) cccTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGga < 4:4388880/149‑1 (MQ=255) ccTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGgat < 4:667910/149‑1 (MQ=255) ccTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGgat > 1:5800890/1‑149 (MQ=255) tCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCgg < 1:2718107/144‑1 (MQ=255) cGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGgatgat < 4:475687/149‑1 (MQ=255) cGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGgatgat < 3:4899236/149‑1 (MQ=255) cGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGgatgat < 2:3662767/149‑1 (MQ=255) gTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATc < 2:1496285/149‑1 (MQ=255) tGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATcc < 4:811555/149‑1 (MQ=255) tGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATcc < 3:6272148/149‑1 (MQ=255) cTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCt < 1:1207874/149‑1 (MQ=255) tttCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCtt > 1:477094/1‑149 (MQ=255) tttCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCtt < 4:4925244/149‑1 (MQ=255) tttCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCtt > 3:2129985/1‑149 (MQ=255) cAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGt < 3:6951875/149‑1 (MQ=255) aaaaTATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGga < 3:2854477/136‑1 (MQ=255) aaaTATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGc > 2:5456219/1‑149 (MQ=255) atatCTTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAg < 4:5549549/149‑1 (MQ=255) cGGTGCAGCTGTGTTTATCGCCCTGACCGGAAAGGTTAACAACGAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGGGGATTCCAGCGCCGGGATGATCCCTTCGTCCAGGCGCAGCggt > 2:119437/1‑147 (MQ=255) cGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGtt < 2:7056005/149‑1 (MQ=255) ggTGAAGATGTCTTTATCGCCGCGACCGTAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCTGGATGATCCCTTCGTGCAGGCACAGCGttt < 3:3417235/149‑1 (MQ=255) ggTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGttt < 2:5797263/149‑1 (MQ=255) ggTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGttt < 3:3684861/149‑1 (MQ=255) gTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGtttt < 3:2512981/149‑1 (MQ=255) tGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTg > 2:1962759/1‑149 (MQ=255) gAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCACGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGa > 2:2899146/1‑149 (MQ=255) aaGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGTGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGaa < 1:915923/149‑1 (MQ=255) aaGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGaa < 4:6888419/149‑1 (MQ=255) aaGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGaa < 2:4223219/149‑1 (MQ=255) aaGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGaa < 1:2543756/149‑1 (MQ=255) aaGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGaa < 3:6785493/149‑1 (MQ=255) gATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAgg < 2:4605779/149‑1 (MQ=255) aTGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGc < 3:19208/149‑1 (MQ=255) tttATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGTAGGCTTCaa > 3:1215415/1‑149 (MQ=255) ttATCGCCGCGACCGGAAAGGTTAACTACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAg < 1:5487168/149‑1 (MQ=255) ttATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGGTTTGAAGGCTTCAAg > 1:3539862/1‑149 (MQ=255) tATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTTGTGCAGGCACAGCGTTTTGAAGGCTTCAAgg > 3:894937/1‑149 (MQ=255) tCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGc < 3:1023997/149‑1 (MQ=255) gccgcGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATc > 4:3011928/1‑109 (MQ=255) ccgcGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAg > 3:5293605/1‑143 (MQ=255) ccgcGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtc < 4:5177489/149‑1 (MQ=255) cgcgACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtca < 2:6534507/149‑1 (MQ=255) cgcgACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtca < 3:3181552/149‑1 (MQ=255) cGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGAGCCCt < 4:5053823/104‑1 (MQ=255) ggAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACTCATGGGCCAGGGCGTGGGAGGATTCCAGCTCCGAGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCgg > 1:5189985/1‑149 (MQ=255) ggAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGTGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAg < 1:1557478/111‑1 (MQ=255) ggAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtcatc < 4:5812960/144‑1 (MQ=255) ggAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtcatc > 3:4304233/1‑144 (MQ=255) ggAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCgg > 3:6130406/1‑149 (MQ=255) gAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGt > 3:4155980/1‑149 (MQ=255) aaaGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAg < 1:2361437/115‑1 (MQ=255) ggTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATa > 1:7210177/1‑149 (MQ=255) gTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAg > 2:4760145/1‑149 (MQ=255) ccaccaGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACg > 3:1687001/1‑149 (MQ=255) caccaGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGt > 1:3869968/1‑149 (MQ=255) caccaGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGt > 4:2080020/1‑149 (MQ=255) caccaGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGt > 4:6987177/1‑149 (MQ=255) aGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGTTTCCAGTGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATa < 1:6633309/138‑1 (MQ=255) aGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAgc > 1:4062781/1‑149 (MQ=255) gctCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGTGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCgg < 1:6049971/126‑1 (MQ=255) gctCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTaa < 1:1993008/140‑1 (MQ=255) ctctTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTcatcat < 4:4087453/122‑1 (MQ=255) ctctTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTc > 4:2282755/1‑149 (MQ=255) tttATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGTCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAg > 4:3363470/1‑149 (MQ=255) tttATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtcatc > 3:2982206/1‑117 (MQ=255) ttATCCGGGTATTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCTTCGGTAATAGACACGTAATCAGCGCGTCCAGt < 4:6098730/149‑1 (MQ=255) aTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGc > 1:3328762/1‑149 (MQ=255) ccGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTg < 2:2143712/149‑1 (MQ=255) cGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGtt < 2:6043069/88‑1 (MQ=255) cGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGt > 2:5123674/1‑149 (MQ=255) ggTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTa < 4:3810330/149‑1 (MQ=255) gTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTaa < 3:3912099/149‑1 (MQ=255) ttttCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAg < 4:2677578/149‑1 (MQ=255) ttCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGTCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGAt < 2:4392727/149‑1 (MQ=255) ttCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGAt < 1:2899146/149‑1 (MQ=255) ttCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGAt < 2:2721355/149‑1 (MQ=255) gcgcATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATAcg > 3:6504862/1‑149 (MQ=255) catcatTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAg > 4:1234763/1‑82 (MQ=255) tcatTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAAt < 2:6367900/117‑1 (MQ=255) ttttCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGtgttgt > 1:59635/1‑149 (MQ=255) tCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTggg < 1:6870190/149‑1 (MQ=255) aCGCATGGTGCAGGGCGTGGGAGGATTCTAGTGCCGGGCTGATACCTTCGGGCAGGCACAGCGTTTTGAACGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGAGCGTTCAGTGCTGATAAGATAAGCGTGGTGTGTGCCg > 2:3462655/1‑149 (MQ=255) gggCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGAc < 4:1658699/149‑1 (MQ=255) gCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACgg < 1:1000312/149‑1 (MQ=255) gCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACgg > 4:636198/1‑149 (MQ=255) gCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACgg < 3:4563934/149‑1 (MQ=255) gCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACgg > 2:738589/1‑149 (MQ=255) ccAGGGCGTGGGAGGATTCCAGTGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACggg < 2:3977230/149‑1 (MQ=255) cAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCt > 2:2597343/1‑114 (MQ=255) cAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGa > 2:6804693/1‑149 (MQ=255) aGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCatcatc > 1:1719774/1‑80 (MQ=255) aGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCatcatc < 2:1719774/80‑1 (MQ=255) gggCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGaaa < 4:4215993/149‑1 (MQ=255) gCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATc < 3:5698057/149‑1 (MQ=255) cGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGTGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGc > 3:5110957/1‑132 (MQ=255) gggAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGt > 1:6324708/1‑149 (MQ=255) ggaggaTTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTc > 4:5342965/1‑149 (MQ=255) gcgcCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGATg > 3:5443721/1‑149 (MQ=255) | AGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCCAGTGCTGTTAAGATACGCGTGTTGTGGGCCGACAGACGGGAAATCCAGTCCGGCGGAGATG > NC_000913/1317224‑1317519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |