Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 36,745 C→T 14.8% P360P (CCG→CCA caiC ← putative crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091336,7450CT14.8% 2106.1 / 286.2 793P360P (CCG→CCAcaiCputative crotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (335/339);  new base T (62/55);  total (399/394)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 793 total aligned reads displayed.

GTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCAAGCGAACGCCG  >  NC_000913/36598‑36892
                                                                                                                                                   |                                                                                                                                                   
gtcgCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCg                                                                                                                                                    >  1:4366954/1‑149 (MQ=255)
 tcgCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCgg                                                                                                                                                   <  1:702857/149‑1 (MQ=255)
     ggtatccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGa                                                                                                                                               <  3:4463466/149‑1 (MQ=255)
     ggtatccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGa                                                                                                                                               <  2:1763129/149‑1 (MQ=255)
       tatccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGAtt                                                                                                                                             <  4:2506970/149‑1 (MQ=255)
        atccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATtg                                                                                                                                            >  4:3009942/1‑149 (MQ=255)
        atccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATtg                                                                                                                                            <  3:6555638/149‑1 (MQ=255)
         tccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATtgt                                                                                                                                           <  1:483764/149‑1 (MQ=255)
          ccggtatcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATtgtg                                                                                                                                          <  1:1053232/149‑1 (MQ=255)
                tcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAtcgtc                                                                                                                                    <  4:6608501/149‑1 (MQ=255)
                tcGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAtcgtc                                                                                                                                    <  4:4571633/149‑1 (MQ=255)
                  gCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTcgc                                                                                                                                  <  3:1356320/149‑1 (MQ=255)
                         tGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGAtctc                                                                                                                           <  3:271566/149‑1 (MQ=255)
                            agccagccATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGc                                                                                                                        >  2:5085029/1‑149 (MQ=255)
                             gccagccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCt                                                                                                                       <  3:5985139/149‑1 (MQ=255)
                              ccagccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCtt                                                                                                                      <  3:6997872/149‑1 (MQ=255)
                               cagccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGCTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTc                                                                                                                     <  3:4687265/149‑1 (MQ=255)
                                agccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCg                                                                                                                    <  3:401903/149‑1 (MQ=255)
                                  ccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGTGCCATTGTGATCGTCGCGGATCTCCGCTTCGtt                                                                                                                  <  2:4879199/149‑2 (MQ=255)
                                  ccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTa                                                                                                                  <  4:4224126/149‑1 (MQ=255)
                                  ccaTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTa                                                                                                                  <  4:2001372/149‑1 (MQ=255)
                                   caTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAg                                                                                                                 >  3:1705357/1‑149 (MQ=255)
                                    aTCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGc                                                                                                                >  2:2064257/1‑149 (MQ=255)
                                       ggCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCaaa                                                                                                             >  4:6080399/1‑149 (MQ=255)
                                          ttCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCgg                                                                                                                                                   >  3:5563591/1‑108 (MQ=255)
                                            ccAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAc                                                                                                           >  4:7077375/1‑146 (MQ=255)
                                             cAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa                                                                                                       >  4:2102519/1‑149 (MQ=255)
                                             cAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa                                                                                                       <  3:6331954/149‑1 (MQ=255)
                                             cAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa                                                                                                       >  1:3671677/1‑149 (MQ=255)
                                              aGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCg                                                                                                                                                    <  4:585992/103‑1 (MQ=255)
                                              aGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCAc                                                                                                      >  4:5605417/1‑149 (MQ=255)
                                                cACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCTATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCAccc                                                                                                    <  2:555564/149‑1 (MQ=255)
                                                 aCTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCg                                                                                                   >  2:741712/1‑149 (MQ=255)
                                                   tttCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCAACCGAc                                                                                                 >  4:3922150/1‑149 (MQ=255)
                                                   tttCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGAc                                                                                                 >  1:5970980/1‑149 (MQ=255)
                                                   tttCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAg                                                                                                                 >  1:2060057/1‑133 (MQ=255)
                                                        cAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATc                                                                                            >  4:6704869/1‑149 (MQ=255)
                                                         aGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCg                                                                                           >  3:5046075/1‑149 (MQ=255)
                                                            ggCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACg                                                                                        <  3:2389568/149‑1 (MQ=255)
                                                             gCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACgg                                                                                       <  2:3810810/149‑1 (MQ=255)
                                                                tgtgGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCa                                                                                    <  1:176517/149‑1 (MQ=255)
                                                                tgtgGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCa                                                                                    <  2:7023864/149‑1 (MQ=255)
                                                                     gTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCTCCCGCCCCACCGACGGCCAGcgtc                                                                               <  4:6461538/149‑1 (MQ=255)
                                                                      ttGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACgg                                                                                       <  3:3246018/140‑1 (MQ=255)
                                                                          gaAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTa                                                                          <  1:1374348/149‑1 (MQ=255)
                                                                                aCTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCa                                                                    <  2:1211683/149‑1 (MQ=255)
                                                                                 ctctTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAg                                                                   >  2:5855973/1‑149 (MQ=255)
                                                                                  tctTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAgg                                                                  >  3:5389215/1‑149 (MQ=255)
                                                                                   ctTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGa                                                                 >  2:4216270/1‑149 (MQ=255)
                                                                                    tttGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGAc                                                                >  3:1913943/1‑149 (MQ=255)
                                                                                      tGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGcgt                                                                                <  1:405938/131‑1 (MQ=255)
                                                                                          gATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGc                                                          >  2:291315/1‑149 (MQ=255)
                                                                                             ggTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGa                                                       <  4:850980/149‑1 (MQ=255)
                                                                                             ggTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGa                                                       >  1:2457522/1‑149 (MQ=255)
                                                                                               ttttCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATa                                                     >  3:6693147/1‑149 (MQ=255)
                                                                                                tttCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATaa                                                    <  2:353714/149‑1 (MQ=255)
                                                                                                  tCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATg                                                  <  1:5541858/149‑1 (MQ=255)
                                                                                                    ccAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGcc                                                <  2:6014994/149‑1 (MQ=255)
                                                                                                     cAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGccc                                               <  1:2717185/149‑1 (MQ=255)
                                                                                                       ggTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAccc                                                                                                         >  1:5791771/1‑89 (MQ=255)
                                                                                                         tATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGa                                                                 >  1:3192581/1‑127 (MQ=255)
                                                                                                         tATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGa                                                                 <  2:3192581/127‑1 (MQ=255)
                                                                                                           tGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATg                                         <  1:3975590/149‑1 (MQ=255)
                                                                                                            gCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATg                                         <  4:2294402/148‑1 (MQ=255)
                                                                                                             ccTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGt                                       <  2:2494562/149‑1 (MQ=255)
                                                                                                              cTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGAc                                                                                                 >  4:1721716/1‑90 (MQ=255)
                                                                                                              cTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGtt                                      >  3:909107/1‑149 (MQ=255)
                                                                                                              cTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGtt                                      >  3:3779265/1‑149 (MQ=255)
                                                                                                               tttAATGCAGATTTCACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGttt                                     >  2:405286/1‑149 (MQ=255)
                                                                                                               tttAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAAt                                                   >  1:1587965/1‑135 (MQ=255)
                                                                                                               tttAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAAt                                                   <  2:1587965/135‑1 (MQ=255)
                                                                                                               tttAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGttt                                     >  3:2144779/1‑149 (MQ=255)
                                                                                                               tttAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGttt                                     >  1:5106177/1‑149 (MQ=255)
                                                                                                                 tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTcc                                   <  4:3779265/149‑1 (MQ=255)
                                                                                                                       aGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATc                             >  4:5141912/1‑149 (MQ=255)
                                                                                                                        gATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATcc                            <  3:4260982/149‑1 (MQ=255)
                                                                                                                         aTTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATccc                           <  2:6372797/149‑1 (MQ=255)
                                                                                                                          tttCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAg                                                                   <  2:6645310/108‑1 (MQ=255)
                                                                                                                          tttCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCa                          >  1:6653506/1‑149 (MQ=255)
                                                                                                                            tCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATa                        >  4:1165257/1‑149 (MQ=255)
                                                                                                                             cACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATaa                       <  3:2982129/149‑1 (MQ=255)
                                                                                                                             cACCGATCTCACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATaa                       <  2:3457578/149‑1 (MQ=255)
                                                                                                                              aCCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAg                      >  1:6513806/1‑149 (MQ=255)
                                                                                                                               ccGATCTCACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGa                     <  2:4418979/149‑1 (MQ=255)
                                                                                                                                cGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGAc                    <  4:1435940/149‑1 (MQ=255)
                                                                                                                                 gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTa                                                                                                                  <  1:6186454/54‑1 (MQ=255)
                                                                                                                                 gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATg                                         >  2:2323692/1‑127 (MQ=255)
                                                                                                                                  aTCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGt                  >  2:4660774/1‑149 (MQ=255)
                                                                                                                                   tctcACCAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTc                 <  4:321958/149‑1 (MQ=255)
                                                                                                                                   tcccACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTc                 >  4:4776322/4‑149 (MQ=255)
                                                                                                                                    ctcACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCa                <  4:2665441/149‑1 (MQ=255)
                                                                                                                                    ctcACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATACCCACAATGGTTTCCGTCATCCCATAAGACGTCa                >  2:5315884/1‑149 (MQ=255)
                                                                                                                                     tcACCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAg               >  4:3471639/1‑149 (MQ=255)
                                                                                                                                       aCCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCa             >  1:3467908/1‑149 (MQ=255)
                                                                                                                                       aCCAGCCGGGAGTGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGATTCCGTCATCCCATTAGACGTCAGCa             >  1:2329585/1‑149 (MQ=255)
                                                                                                                                       aCCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCa             >  1:79880/1‑149 (MQ=255)
                                                                                                                                        ccAGCCGGGAGCGGGCGATTGTGAACGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACaa                                           <  2:932226/118‑1 (MQ=255)
                                                                                                                                          aGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCAAGc          >  1:5350295/1‑149 (MQ=255)
                                                                                                                                          aGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCAAGc          <  3:3496163/149‑1 (MQ=255)
                                                                                                                                              gggAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCAAGCGAAc      <  1:845716/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCTTGTGGGTTGAGAAAGTACTCTTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTTTATCGCCAGGACGATCGCCGATAATGCCCACAATGGTTTCCGTCATCCCATAAGACGTCAGCAAGCGAACGCCG  >  NC_000913/36598‑36892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: