Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,450,208 C→T 17.9% G381D (GGC→GAC)  tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,450,2080CT17.9% 1930.3 / inf 808G381D (GGC→GAC) tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (314/348);  new base T (68/76);  total (384/424)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 825 total aligned reads displayed.

TTTACCACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGC  >  NC_000913/1450060‑1450356
                                                                                                                                                    |                                                                                                                                                    
  tACCACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCa                                                                                                                                                    >  1:737679/1‑149 (MQ=255)
    ccACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCAtt                                                                                                                                                  >  3:3577967/1‑149 (MQ=255)
    ccACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCAtt                                                                                                                                                  >  2:1888597/1‑149 (MQ=255)
     cACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTg                                                                                                                                                 >  3:5935147/1‑149 (MQ=255)
     cACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTg                                                                                                                                                 >  1:5313810/1‑149 (MQ=255)
         aGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGt                                                                                                                                             <  3:6762599/149‑1 (MQ=255)
         aGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGt                                                                                                                                             <  2:4486336/149‑1 (MQ=255)
          gCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGtt                                                                                                                                            <  4:2796763/149‑1 (MQ=255)
                  tGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCa                                                                                                                                    >  2:2147081/1‑149 (MQ=255)
                  tGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCa                                                                                                                                    <  1:2384840/149‑1 (MQ=255)
                     ggTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACgg                                                                                                                                 <  3:7003083/149‑1 (MQ=255)
                         aGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGga                                                                                                                             <  1:2763024/149‑1 (MQ=255)
                           cGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGgaga                                                                                                                           <  2:27884/149‑1 (MQ=255)
                           cGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGgaga                                                                                                                           <  2:292979/149‑1 (MQ=255)
                                ccATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGatcatc                                                                                                                      >  1:321099/1‑149 (MQ=255)
                                ccATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGatcatc                                                                                                                      <  4:168561/149‑1 (MQ=255)
                                ccATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGatcatc                                                                                                                      >  1:4844283/1‑149 (MQ=255)
                                 cATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGTATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCg                                                                                                                     <  1:6178054/149‑1 (MQ=255)
                                  aTACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCgg                                                                                                                    >  3:1499778/1‑149 (MQ=255)
                                    aCCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGcc                                                                                                                  >  4:4802143/1‑149 (MQ=255)
                                    aCCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGcc                                                                                                                  >  4:2324060/1‑149 (MQ=255)
                                            cACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCga                                                                                                          <  3:6265795/149‑1 (MQ=255)
                                             aCCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCgag                                                                                                         >  2:6390105/1‑149 (MQ=255)
                                             aCCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCACCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCgag                                                                                                         >  4:6265047/1‑149 (MQ=255)
                                              ccAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGTACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCgaga                                                                                                        <  2:5975999/149‑1 (MQ=255)
                                              ccAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCTCGGTGGAGATCATCGGCCCGACGCgaga                                                                                                        <  3:1969194/149‑1 (MQ=255)
                                              ccAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCgaga                                                                                                        <  3:5128984/149‑1 (MQ=255)
                                               cAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGagag                                                                                                       >  4:7105355/1‑149 (MQ=255)
                                                 gagTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGtt                                                                                                     >  3:2611584/1‑149 (MQ=255)
                                                  agTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTc                                                                                                    <  3:2486453/149‑1 (MQ=255)
                                                          tACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAg                                                                                            >  1:6487892/1‑149 (MQ=255)
                                                             gCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTg                                                                                         >  4:1068821/1‑149 (MQ=255)
                                                               tttAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGaa                                                                                       >  1:100490/1‑149 (MQ=255)
                                                               tttAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGaa                                                                                       >  1:5804581/1‑149 (MQ=255)
                                                                  aaaGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAAt                                                                                    <  4:2540744/149‑1 (MQ=255)
                                                                    aGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATcc                                                                                  <  4:3577967/149‑1 (MQ=255)
                                                                     gTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTta                                                                                                                                           <  4:1256439/91‑1 (MQ=255)
                                                                     gTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATccc                                                                                 <  2:7152031/149‑1 (MQ=255)
                                                                      tACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCa                                                                                <  1:1543394/149‑1 (MQ=255)
                                                                         cAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGtt                                                                             >  3:655339/1‑149 (MQ=255)
                                                                         cAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGtt                                                                             <  3:4732979/149‑1 (MQ=255)
                                                                           gCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTc                                                                                                    <  4:5330973/124‑1 (MQ=255)
                                                                           gCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTcc                                                                           >  3:1098110/1‑149 (MQ=255)
                                                                            ccAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCg                                                                          >  2:3941897/1‑149 (MQ=255)
                                                                                tatCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATaa                                                                                                                                        >  2:4857885/1‑83 (MQ=255)
                                                                                 atCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGGACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGt                                                                     >  4:3869413/1‑149 (MQ=255)
                                                                                 atCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGt                                                                     <  1:3941897/149‑1 (MQ=255)
                                                                                 atCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGt                                                                     >  1:3601453/1‑149 (MQ=255)
                                                                                  tCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCa                                                                                <  4:6563705/137‑1 (MQ=255)
                                                                                    aGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGaa                                                                  <  3:1376938/149‑1 (MQ=255)
                                                                                     ggATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAAt                                                                 <  3:4233483/149‑1 (MQ=255)
                                                                                      gATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGc                                                                                                >  2:4668853/1‑117 (MQ=255)
                                                                                      gATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATc                                                                >  4:334805/1‑149 (MQ=255)
                                                                                      gATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATc                                                                >  2:1006242/1‑149 (MQ=255)
                                                                                      gATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATc                                                                >  3:5331981/1‑149 (MQ=255)
                                                                                        tCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCat                                                              >  2:1554634/1‑149 (MQ=255)
                                                                                          actgTAAGGCACTATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCatat                                                            <  1:5687418/146‑1 (MQ=255)
                                                                                            cGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCg                                                          >  1:4609403/1‑149 (MQ=255)
                                                                                             gTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGc                                                         <  1:2959542/149‑1 (MQ=255)
                                                                                                 agCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGTGTTTGGTGCCATGGTCGTtat                                                                                                                                          >  4:6106671/2‑64 (MQ=255)
                                                                                                    acaATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTg                                                                    <  4:5133433/131‑1 (MQ=255)
                                                                                                    acaATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGt                                                                                                      >  4:6716293/1‑97 (MQ=255)
                                                                                                    acaATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGt                                                                     <  1:1255723/130‑1 (MQ=255)
                                                                                                    acaATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGt                                                                     >  2:1255723/1‑130 (MQ=255)
                                                                                                    acaATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAAt                                                  >  2:3433392/1‑149 (MQ=255)
                                                                                                    acaATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAAt                                                  >  2:4134827/1‑149 (MQ=255)
                                                                                                     caATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAg                                                                                            <  1:2626306/106‑1 (MQ=255)
                                                                                                       aTCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGgt                                               <  1:7057335/149‑1 (MQ=255)
                                                                                                       aTCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGgt                                               <  3:4466895/149‑1 (MQ=255)
                                                                                                       aTCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGgt                                               <  4:4080307/149‑1 (MQ=255)
                                                                                                          aTGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCa                                                                                <  2:6955246/113‑1 (MQ=255)
                                                                                                             ccgccgAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAAtt                                         <  4:5842996/149‑1 (MQ=255)
                                                                                                               gccgAGAGAACCTTCGTACATGACTTTGCGTTTGGTGTCATTGCCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAAtttt                                       >  2:3251854/1‑149 (MQ=255)
                                                                                                               gccgAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAAtttt                                       <  2:825066/149‑1 (MQ=255)
                                                                                                                  gagagaACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTAc                                    >  1:1969093/1‑149 (MQ=255)
                                                                                                                  gagagaACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTAc                                    <  3:5012189/149‑1 (MQ=255)
                                                                                                                     agaACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGAcgc                                                                                                            >  3:3833641/1‑74 (MQ=255)
                                                                                                                      gaACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTc                                <  3:3006198/149‑1 (MQ=255)
                                                                                                                        aCCTTCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGatc                                                                                                                         <  1:5912965/58‑1 (MQ=255)
                                                                                                                        aCCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAg                              <  2:3715410/149‑1 (MQ=255)
                                                                                                                           ttCGTACATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTc                                                                                                    <  1:4186868/76‑1 (MQ=255)
                                                                                                                             cGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCa                         <  3:5577432/149‑1 (MQ=255)
                                                                                                                              gTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCaa                        <  4:3029999/149‑1 (MQ=255)
                                                                                                                              gTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCaa                        <  4:1402192/149‑1 (MQ=255)
                                                                                                                               tACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAAt                       >  4:6910500/1‑149 (MQ=255)
                                                                                                                                aCATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATg                      >  3:754807/1‑149 (MQ=255)
                                                                                                                                 cATGACTTTGCGTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGa                     >  2:5603823/1‑149 (MQ=255)
                                                                                                                                  aTGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCCCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGAt                    >  4:555652/1‑149 (MQ=255)
                                                                                                                                   tGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGAtt                   <  1:5009496/149‑1 (MQ=255)
                                                                                                                                      cTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGa                     <  4:452010/144‑1 (MQ=255)
                                                                                                                                       tttGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCa               <  2:2659159/149‑1 (MQ=255)
                                                                                                                                         tGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATa             <  1:6863942/149‑1 (MQ=255)
                                                                                                                                            gTTTGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGc          <  2:2483138/149‑1 (MQ=255)
                                                                                                                                            gTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGc          <  3:3146644/149‑1 (MQ=255)
                                                                                                                                             tttGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGGATAGGCt         >  3:6128899/1‑149 (MQ=255)
                                                                                                                                              ttGGTGTCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCtt        <  2:4235865/149‑1 (MQ=255)
                                                                                                                                                   gCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATa             <  3:223122/139‑1 (MQ=255)
                                                                                                                                                    ccATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGc  >  1:3339197/1‑149 (MQ=255)
                                                                                                                                                    ccATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGc  >  2:6682103/1‑149 (MQ=255)
                                                                                                                                                    ccATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGc  <  2:3120017/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTACCACGAGCAATTGGTGAGGTTAGCGTGCCCATACCGTAGTCACCAGAGTCCAGATACGCTTTAAAGTACCAGCCAATATCAGGATCACCGTAAGGCACAATCATGCCGCCGAGAGAACCTTCGTACATGACTTTGCGTTTGGTGCCATTGTCGTTATAAGTCACGGTGGAGATCATCGGCCCGACGCGAGAGTTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGC  >  NC_000913/1450060‑1450356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: