BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,812,089	G→A	14.3%	V386I (GTT→ATT)	ydjN →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,089	0	G	A	14.3%	1782.6 / 233.1	662	V386I (GTT→ATT)	ydjN	putative transporter
Reads supporting (aligned to +/- strand): ref base G (263/304); new base A (43/52); total (306/356)
Fisher's exact test for biased strand distribution p-value = 9.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 669 total aligned reads displayed.

TTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGAC  >  NC_000913/1811941‑1812237

   cGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTg                                                                                                                                                   >  3:4393048/1‑149 (MQ=255)

     gTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGcc                                                                                                                                                 <  2:5948673/149‑1 (MQ=255)

      tGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCg                                                                                                                                                <  2:4710686/149‑1 (MQ=255)

      tGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCg                                                                                                                                                <  2:5018736/149‑1 (MQ=255)

      tGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCg                                                                                                                                                <  3:408040/149‑1 (MQ=255)

       gCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCgg                                                                                                                                               >  4:4673380/1‑149 (MQ=255)

         aaCCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGgtg                                                                                                                                             >  3:2276534/1‑149 (MQ=255)

         aaCCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGgtg                                                                                                                                             >  4:5271859/1‑149 (MQ=255)

             aTTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCgg                                                                                                                                         <  3:1775505/149‑1 (MQ=255)

             aTTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCgg                                                                                                                                         <  1:4296818/149‑1 (MQ=255)

               tGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCggtg                                                                                                                                       <  3:1001574/149‑1 (MQ=255)

               tGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCggtg                                                                                                                                       <  4:1837383/149‑1 (MQ=255)

                ggTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCggtgg                                                                                                                                      <  4:3893785/149‑1 (MQ=255)

                ggTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCggtgg                                                                                                                                      <  2:1373455/149‑1 (MQ=255)

                  tCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCggtggtg                                                                                                                                    <  2:119049/149‑1 (MQ=255)

                  tCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCACTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCggtggtg                                                                                                                                    <  2:2126338/149‑1 (MQ=255)

                     gAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGgtggtggtg                                                                                                                                 >  4:1296959/1‑149 (MQ=255)

                      aaCGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGgt                                                                                                                                              >  2:1972133/1‑135 (MQ=255)

                              cgcCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTcg                                                                                                                        >  1:6778532/1‑149 (MQ=255)

                               gcCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTcgc                                                                                                                       <  1:3771038/149‑1 (MQ=255)

                                     ttGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACt                                                                                                                 >  3:6333601/1‑149 (MQ=255)

                                       gTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGGCGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGa                                                                                                               <  2:4444230/149‑1 (MQ=255)

                                       gTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGa                                                                                                               <  3:256955/149‑1 (MQ=255)

                                       gTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGa                                                                                                               <  1:5002744/149‑1 (MQ=255)

                                        tATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGAt                                                                                                              >  3:6999253/1‑149 (MQ=255)

                                         aTCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGAtt                                                                                                             >  2:608670/1‑149 (MQ=255)

                                            cGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTa                                                                                                          <  4:7087683/149‑1 (MQ=255)

                                            cGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTa                                                                                                          >  4:3634064/1‑149 (MQ=255)

                                             ggCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTAc                                                                                                         >  1:532429/1‑149 (MQ=255)

                                                  tGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTActgcct                                                                                                    <  1:6011824/149‑1 (MQ=255)

                                                     tGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCg                                                                                                 >  1:1628851/1‑149 (MQ=255)

                                                        cGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATg                                                                                              <  4:4066898/149‑1 (MQ=255)

                                                         ggTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATgg                                                                                             <  3:1964954/149‑1 (MQ=255)

                                                             aTGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCt                                                                                         <  2:6511163/149‑1 (MQ=255)

                                                             aTGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCt                                                                                         >  1:1026123/1‑149 (MQ=255)

                                                             aTGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCt                                                                                         >  1:7086435/1‑149 (MQ=255)

                                                               ggTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGc                                                                                       <  1:4043038/149‑1 (MQ=255)

                                                                gTTGCGCCTACGGTGGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGcc                                                                                      >  3:550256/1‑149 (MQ=255)

                                                                 ttGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCa                                                                                     >  3:3045655/1‑149 (MQ=255)

                                                                 ttGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTcgc                                                                                                                       >  2:6283485/1‑115 (MQ=255)

                                                                   gcgcCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGTCTGCCAGt                                                                                   >  4:3379763/1‑149 (MQ=255)

                                                                      ccTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAAc                                                                                >  2:3877105/1‑149 (MQ=255)

                                                                       cTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAAcc                                                                               >  3:379449/1‑149 (MQ=255)

                                                                           ggTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCtgg                                                                           <  2:5606156/149‑1 (MQ=255)

                                                                            gTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCtggt                                                                          <  2:2283340/149‑1 (MQ=255)

                                                                               ggCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGgc                                                                       >  1:2994088/1‑149 (MQ=255)

                                                                                gCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGgcg                                                                      >  1:5623032/1‑149 (MQ=255)

                                                                                gCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGgcg                                                                      >  4:2634135/1‑149 (MQ=255)

                                                                                 cATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGgcgc                                                                     <  3:3302385/149‑1 (MQ=255)

                                                                                   ttAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTg                                                                   >  2:5314923/1‑149 (MQ=255)

                                                                                   ttAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTg                                                                   <  2:3125883/149‑1 (MQ=255)

                                                                                    tAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGt                                                                  <  1:3264689/149‑1 (MQ=255)

                                                                                     aaCCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTActgc                                                                                                      <  1:2788536/112‑1 (MQ=255)

                                                                                       cccGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTaa                                                               >  1:3656364/1‑149 (MQ=255)

                                                                                        ccGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAAt                                                              >  4:5628832/1‑149 (MQ=255)

                                                                                        ccGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAAt                                                              >  2:3715422/1‑149 (MQ=255)

                                                                                         cGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAAtc                                                             <  4:6701316/149‑1 (MQ=255)

                                                                                         cGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAAtc                                                             >  2:1646229/1‑149 (MQ=255)

                                                                                            tGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTcc                                                          <  4:4445812/149‑1 (MQ=255)

                                                                                              gACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGt                                                        <  2:7086435/149‑1 (MQ=255)

                                                                                              gACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCTGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGt                                                        <  4:5000464/149‑1 (MQ=255)

                                                                                              gACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGt                                                        <  4:225767/149‑1 (MQ=255)

                                                                                               aCCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGtt                                                       <  3:5269879/149‑1 (MQ=255)

                                                                                                cccGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTg                                                      >  2:3470086/1‑149 (MQ=255)

                                                                                                cccGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCg                                                         <  1:875080/146‑1 (MQ=255)

                                                                                                 ccGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGa                                                     >  1:1148703/1‑149 (MQ=255)

                                                                                                   gATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAAc                                                   <  1:2328516/149‑1 (MQ=255)

                                                                                                    aTGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAAcc                                                  >  1:4740886/1‑149 (MQ=255)

                                                                                                               gACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGAtt                                                                                                             >  1:6243496/1‑79 (MQ=255)

                                                                                                               gACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCtg                                                                            <  3:4222744/112‑1 (MQ=255)

                                                                                                               gACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACa                                       >  3:6544950/1‑149 (MQ=255)

                                                                                                                aCGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACAt                                      <  4:5788694/149‑1 (MQ=255)

                                                                                                                aCGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCtggtg                                                                         >  2:4356645/1‑114 (MQ=255)

                                                                                                                   cTGGTCGGTATTGTTACCGTTAGTTCCGCAGTCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGGCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATggg                                   <  1:2078337/149‑1 (MQ=255)

                                                                                                                         ggTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTAc                             <  2:4204489/149‑1 (MQ=255)

                                                                                                                          gTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGCGGTGCAACTTTCGCCGCACTGAt                                                                                                              >  3:3596898/1‑67 (MQ=255)

                                                                                                                            aTTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGc                          <  2:6786459/149‑1 (MQ=255)

                                                                                                                             ttGTTACCGTTAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCg                         <  2:734074/149‑1 (MQ=255)

                                                                                                                              tGTTACCGTTAGTTCCGCAGGCGTTGACGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGt                        >  3:5978304/1‑149 (MQ=255)

                                                                                                                                 tACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTaa                     >  3:1313530/1‑149 (MQ=255)

                                                                                                                                  aCCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTaaa                    <  4:6385426/149‑1 (MQ=255)

                                                                                                                                  aCCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTaaa                    <  4:3219841/149‑1 (MQ=255)

                                                                                                                                   ccGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAAc                   <  3:5836907/149‑1 (MQ=255)

                                                                                                                                   ccGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAAc                   <  4:4280540/149‑1 (MQ=255)

                                                                                                                                     gTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt                 >  3:353791/1‑149 (MQ=255)

                                                                                                                                     gTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt                 >  1:6442815/1‑149 (MQ=255)

                                                                                                                                      ttAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGtt                >  3:966268/1‑149 (MQ=255)

                                                                                                                                      ttAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGtt                >  2:2870221/1‑149 (MQ=255)

                                                                                                                                      ttAGTTCCGCAGGCATTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGtt                >  1:4440469/1‑149 (MQ=255)

                                                                                                                                       tAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTa               <  1:2550098/149‑1 (MQ=255)

                                                                                                                                        aGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAg              >  3:6769077/1‑149 (MQ=255)

                                                                                                                                         gTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGt             >  3:472202/1‑149 (MQ=255)

                                                                                                                                          ttCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTg            >  2:2373470/1‑149 (MQ=255)

                                                                                                                                          ttCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTg            <  3:2761391/149‑1 (MQ=255)

                                                                                                                                          ttCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTg            <  4:6850041/149‑1 (MQ=255)

                                                                                                                                            ccGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt                 >  4:4814520/1‑142 (MQ=255)

                                                                                                                                                aGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGa      <  3:1178224/149‑1 (MQ=255)

                                                                                                                                                 ggCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCTCTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGAt     <  2:265724/149‑1 (MQ=255)

                                                                                                                                                    gTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGa   <  4:5923770/148‑1 (MQ=255)

                                                                                                                                                    gTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGAc  <  4:2838712/149‑1 (MQ=255)

TTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGGTTTGTATCCGGCAATGCTGGCGGTGATGGTTGCGCCTACGGTTGGCATTAACCCGCTGGACCCGATGTGGATTGCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGAC  >  NC_000913/1811941‑1812237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`