Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,216,569 G→A 5.4% A280V (GCG→GTG)  yehX ← putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,216,5690GA 5.4% 2081.3 / 61.7 629A280V (GCG→GTG) yehXputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (315/278);  new base A (18/16);  total (334/295)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

Only 100 of 644 total aligned reads displayed.

ATCAGCGCCACAAACAGAGCAATGAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGAC  >  NC_000913/2216421‑2216717
                                                                                                                                                    |                                                                                                                                                    
aTCAGCGCCACGAACAGAGCAATGAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCcgcg                                                                                                                                                      >  1:223497/1‑149 (MQ=255)
             aCAGAGCAATGAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACagaga                                                                                                                                         <  3:1143706/149‑1 (MQ=255)
             aCAGAGCAATGAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACagaga                                                                                                                                         <  4:2474548/149‑1 (MQ=255)
               agagCAATGAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCACAACAAACagagaga                                                                                                                                       <  3:1394939/149‑1 (MQ=255)
                       gAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATcg                                                                                                                               <  4:108206/149‑1 (MQ=255)
                         gCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATcgcg                                                                                                                             >  2:3778048/1‑149 (MQ=255)
                         gCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATcgcg                                                                                                                             <  4:5289493/149‑1 (MQ=255)
                          ccAGAACGGCGGATCGGGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGt                                                                                                                            >  2:416154/1‑149 (MQ=255)
                          ccAGAACAGCGGATCGCGCAACATCTTCATATGCTTACCCCTCCACCAGCAGATCCTGAAAAGGCAACGTTCCGCAAGGCAGCCCCCGCAATCTCACAACCCGCAGCACCTCGTATTCCCGCGCCCCAAGCACAGAGAACGTATCTCGt                                                                                                                            >  2:6088138/1‑149 (MQ=255)
                          ccAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGt                                                                                                                            <  2:5819877/149‑1 (MQ=255)
                            aGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAg                                                                                                                          <  2:1969337/149‑1 (MQ=255)
                             gAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGc                                                                                                                         >  4:1730972/1‑149 (MQ=255)
                              aaCAGCGGATCGCGCAACATCTTCATACGCTTACGCGTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGGAGCACCTCGCATCCCCGCGCAACAAACAGAGTGAGCGCATCGCGTAGCg                                                                                                                        >  4:6012493/1‑149 (MQ=255)
                                cAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTc                                                                                                                      <  3:2235777/149‑1 (MQ=255)
                                  gCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCAt                                                                                                                    <  1:3867506/149‑1 (MQ=255)
                                  gCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCAt                                                                                                                    >  4:6769256/1‑149 (MQ=255)
                                   cGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCAtc                                                                                                                   <  3:661943/149‑1 (MQ=255)
                                    ggATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCAtct                                                                                                                  >  3:7048988/1‑149 (MQ=255)
                                     gATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGCGCGCATCGCGTAGCGTCAtctc                                                                                                                 >  2:5486478/1‑149 (MQ=255)
                                     gATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGc                                                                                                                         <  2:2737334/141‑1 (MQ=255)
                                        cgcgCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCtcttc                                                                                                              <  3:6389629/149‑1 (MQ=255)
                                        cgcgCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCtcttc                                                                                                              <  4:656006/149‑1 (MQ=255)
                                           gcAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGc                                                                                                           >  3:426034/1‑149 (MQ=255)
                                           gcAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGc                                                                                                           <  2:1301679/149‑1 (MQ=255)
                                             aaCATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCCTCTCTACTGCCa                                                                                                         >  4:3436913/1‑149 (MQ=255)
                                             aaCATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGc                                                                                                           <  2:1414896/147‑1 (MQ=255)
                                             aaCATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGc                                                                                                           >  1:1414896/1‑147 (MQ=255)
                                             aaCATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCa                                                                                                         <  2:230091/149‑1 (MQ=255)
                                                aTCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTg                                                                                                      >  3:5402595/1‑149 (MQ=255)
                                                 tgttCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGTCTGGCCCTGCATGTTCACCACCGGCACCACCTCGCATCCCCGCGCAACAAACAGACAGAGCGCATCTCGTCGCGTCTTCTCTTCTGCCAGTGc                                                                                                     >  4:3598906/3‑149 (MQ=255)
                                                      aTACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCAc                                                                                                >  4:771393/1‑149 (MQ=255)
                                                      aTACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCAc                                                                                                >  4:4763550/1‑149 (MQ=255)
                                                      aTACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCAc                                                                                                >  4:6392626/1‑149 (MQ=255)
                                                       tACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTc                                                                                                  >  2:4053756/1‑146 (MQ=255)
                                                         cGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCAt                                                                                             <  4:1460331/149‑1 (MQ=255)
                                                          gCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATc                                                                                            >  2:4477176/1‑149 (MQ=255)
                                                          gCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATc                                                                                            <  1:6633471/149‑1 (MQ=255)
                                                          gCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATc                                                                                            >  2:2100388/1‑149 (MQ=255)
                                                          gCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTACCAGTGCCTCACCATc                                                                                            <  4:5669905/149‑1 (MQ=255)
                                                              aCGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCt                                                                                        <  1:3281183/149‑1 (MQ=255)
                                                               cGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTc                                                                                       <  3:448533/149‑1 (MQ=255)
                                                               cGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTc                                                                                       <  4:5004425/149‑1 (MQ=255)
                                                                  cTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGtt                                                                                    >  2:3563527/1‑149 (MQ=255)
                                                                  cTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGtt                                                                                    >  1:4365553/1‑149 (MQ=255)
                                                                   tCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCACAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGttc                                                                                   >  3:3089894/1‑149 (MQ=255)
                                                                      accaGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCACAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGttcttc                                                                                >  4:6200777/1‑149 (MQ=255)
                                                                          gcagATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGa                                                                            >  3:2434870/1‑149 (MQ=255)
                                                                          gcagATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGa                                                                            >  1:6423455/1‑149 (MQ=255)
                                                                             gATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGc                                                                         >  1:4611409/1‑149 (MQ=255)
                                                                             gATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGc                                                                         <  3:3254501/149‑1 (MQ=255)
                                                                              aTCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTTTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCa                                                                        >  1:73506/1‑149 (MQ=255)
                                                                                 cTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGt                                                                     >  2:1703338/1‑149 (MQ=255)
                                                                                 cTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGt                                                                     >  4:2794921/1‑149 (MQ=255)
                                                                                  tGAAAATGCAGCGTGCCGCAAGGCTGGCGCTACATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGAGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTa                                                                    >  4:4369484/1‑149 (MQ=255)
                                                                                   gAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCTGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGCTCTTCGCGACGCACGTaa                                                                   >  3:3981055/1‑149 (MQ=255)
                                                                                   gAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTaa                                                                   >  4:1288953/1‑149 (MQ=255)
                                                                                   gAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTaa                                                                   >  3:5674535/1‑149 (MQ=255)
                                                                                      aaTGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATcc                                                                >  3:3698712/1‑149 (MQ=255)
                                                                                      aaTGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGAACGTAATcc                                                                >  4:1970228/1‑149 (MQ=255)
                                                                                       aTGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGttc                                                                                   >  3:5531244/1‑129 (MQ=255)
                                                                                             cGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCacac                                                         <  4:315763/149‑1 (MQ=255)
                                                                                                gCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCACAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTAc                                                      <  1:1053996/149‑1 (MQ=255)
                                                                                                 ccGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACg                                                     <  1:1816170/149‑1 (MQ=255)
                                                                                                  cGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGt                                                    >  1:6012287/1‑149 (MQ=255)
                                                                                                   gCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCcaca                                                          >  4:2869606/1‑142 (MQ=255)
                                                                                                          tGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAg                                            <  3:259387/149‑1 (MQ=255)
                                                                                                            gCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCACAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCa                                          <  3:6809641/149‑1 (MQ=255)
                                                                                                            gCCCTGCATGGTCACCACCGGCAGAACCTCGCTTCCCAGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCGTCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCTACACTAAGTAACGAAAGCa                                          >  2:2904916/1‑149 (MQ=255)
                                                                                                             cccTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAg                                         <  2:2434052/149‑1 (MQ=255)
                                                                                                             cccTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAg                                         <  3:1979850/149‑1 (MQ=255)
                                                                                                               cTGCATGTTCACCACCTGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCGCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGgc                                       >  4:5532304/1‑149 (MQ=255)
                                                                                                               cTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGgc                                       >  2:1117106/1‑149 (MQ=255)
                                                                                                               cTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGgc                                       <  3:788514/149‑1 (MQ=255)
                                                                                                               cTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGgc                                       <  1:4774910/149‑1 (MQ=255)
                                                                                                                 gCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGgcgc                                     >  4:21193/1‑149 (MQ=255)
                                                                                                                    tGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGc                                                                                                                         >  1:4119678/1‑62 (MQ=255)
                                                                                                                    tGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCtctt                                                                                                               <  1:4938686/72‑1 (MQ=255)
                                                                                                                      ttCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACAcc                                >  3:2790548/1‑149 (MQ=255)
                                                                                                                       tCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACAccc                               >  3:6015176/1‑149 (MQ=255)
                                                                                                                          ccaccGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGt                            >  1:5486093/1‑149 (MQ=255)
                                                                                                                          ccaccGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGt                            >  2:4892529/1‑149 (MQ=255)
                                                                                                                          ccaccGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGt                            >  2:799461/1‑149 (MQ=255)
                                                                                                                              cGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCAc                        <  2:5046342/149‑1 (MQ=255)
                                                                                                                                   gcaCCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGt                   >  2:4620425/1‑149 (MQ=255)
                                                                                                                                     aCCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTcc                 >  1:2033595/1‑149 (MQ=255)
                                                                                                                                     aCCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTcc                 >  1:5726390/1‑149 (MQ=255)
                                                                                                                                      ccTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCa                >  3:5090304/1‑149 (MQ=255)
                                                                                                                                      ccTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCa                >  2:7051424/1‑149 (MQ=255)
                                                                                                                                      ccTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCa                >  1:5103394/1‑149 (MQ=255)
                                                                                                                                       cTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATc                                                                                            <  3:6470950/72‑1 (MQ=255)
                                                                                                                                       cTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTTCGTCCaa               >  2:6664354/1‑149 (MQ=255)
                                                                                                                                        tCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGc                                           <  3:1692092/120‑1 (MQ=255)
                                                                                                                                         cGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCGTCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCaaaa             <  4:4540657/149‑1 (MQ=255)
                                                                                                                                         cGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCaaaa             <  2:3346122/149‑1 (MQ=255)
                                                                                                                                          gCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCaaaaa            >  2:6492818/1‑149 (MQ=255)
                                                                                                                                          gCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCaaaaa            >  4:3835372/1‑149 (MQ=255)
                                                                                                                                              ccccGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTg        <  1:3576495/149‑1 (MQ=255)
                                                                                                                                                ccGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGc      <  4:40888/149‑1 (MQ=255)
                                                                                                                                                 cgcgCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCg     >  1:6948706/1‑149 (MQ=255)
                                                                                                                                                  gcgcAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCgg    >  1:2879343/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCAGCGCCACAAACAGAGCAATGAGCCAGAACAGCGGATCGCGCAACATCTTCATACGCTTACGCCTCCACCAGCAGATCCTGAAAATGCAGCGTGCCGCAAGGCTGGCCCTGCATGTTCACCACCGGCAGCACCTCGCATCCCCGCGCAACAAACAGAGAGAGCGCATCGCGTAGCGTCATCTCTTCTGCCAGTGCCTCACCATCTGCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGAC  >  NC_000913/2216421‑2216717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: