Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,966,582 C→T 5.9% G618D (GGC→GAC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,966,5820CT 5.9% 2668.0 / 93.4 819G618D (GGC→GAC) ptsPPEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base C (394/376);  new base T (23/26);  total (417/402)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 831 total aligned reads displayed.

CAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCG  >  NC_000913/2966434‑2966730
                                                                                                                                                    |                                                                                                                                                    
 aaaCGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGcc                                                                                                                                                     >  1:1787604/1‑149 (MQ=255)
     ggAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACa                                                                                                                                                 <  3:438188/149‑1 (MQ=255)
     ggAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACa                                                                                                                                                 <  4:4835041/149‑1 (MQ=255)
      gagaTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACag                                                                                                                                                >  1:45510/1‑149 (MQ=255)
               tCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAAt                                                                                                                                       <  1:5098579/149‑1 (MQ=255)
               tCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAACACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAAt                                                                                                                                       <  4:2576902/149‑1 (MQ=255)
                 cATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCg                                                                                                                                     >  1:2286616/1‑149 (MQ=255)
                   tGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGAc                                                                                                                                   <  2:1179017/149‑1 (MQ=255)
                   tGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGAc                                                                                                                                   <  1:3755107/149‑1 (MQ=255)
                   tGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGCTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGAc                                                                                                                                   <  3:6371699/149‑1 (MQ=255)
                      aTTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCg                                                                                                                                >  1:4735582/1‑149 (MQ=255)
                       tttCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGcc                                                                                                                                                     <  1:2629372/127‑1 (MQ=255)
                       tttCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGcc                                                                                                                                                     >  2:2629372/1‑127 (MQ=255)
                       tttCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACagagtt                                                                                                                                            <  4:1177469/136‑3 (MQ=255)
                       tttCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGc                                                                                                                               <  2:4507487/149‑1 (MQ=255)
                        ttCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCt                                                                                                                              <  4:6532004/149‑1 (MQ=255)
                         tCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCtt                                                                                                                             >  1:7211194/1‑149 (MQ=255)
                          ccGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCttt                                                                                                                            >  3:3818954/1‑149 (MQ=255)
                                 ccGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGAt                                                                                                                     <  2:2311455/149‑1 (MQ=255)
                                   gggCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGc                                                                                                                   <  3:1457154/149‑1 (MQ=255)
                                       gATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgca                                                                                                               >  4:7052149/1‑149 (MQ=255)
                                         tcatcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGa                                                                                                                      >  3:2887343/1‑140 (MQ=255)
                                          catcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgcagca                                                                                                            >  1:1026838/1‑149 (MQ=255)
                                           atcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGAt                                                                                                                                            <  2:2065391/116‑1 (MQ=255)
                                              gCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATaaa                                                                                                        >  2:6588211/1‑149 (MQ=255)
                                               ccAGAGCTCGTAACATTACAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAAt                                                                                                       <  2:6804163/149‑1 (MQ=255)
                                                cAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATa                                                                                                      >  4:3126648/1‑149 (MQ=255)
                                                cAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATa                                                                                                      <  2:999254/149‑1 (MQ=255)
                                                cAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATa                                                                                                      >  1:3446035/1‑149 (MQ=255)
                                                        gTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGAc                                                                                              <  3:4631766/149‑1 (MQ=255)
                                                        gTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGAc                                                                                              >  3:1299243/1‑149 (MQ=255)
                                                          aaCATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGAc                                                                                              >  1:4044420/1‑147 (MQ=255)
                                                             aTTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCAc                                                                                         >  3:7024146/1‑149 (MQ=255)
                                                             aTTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCAc                                                                                         <  1:2129468/149‑1 (MQ=255)
                                                                gCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCAt                                                                                                           >  3:488265/1‑128 (MQ=255)
                                                                  aGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                                         <  3:2025583/112‑1 (MQ=255)
                                                                  aGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGCTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCa                                                                                    <  4:5820577/149‑1 (MQ=255)
                                                                    gATGTAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCAACCCGCTTTGCCAGATCCGGCAGCACAAATACCATTGACGGCACTTCCAGc                                                                                  >  2:607673/1‑149 (MQ=255)
                                                                    gATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGc                                                                                  <  4:3813369/149‑1 (MQ=255)
                                                                    gATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGc                                                                                  >  2:5744134/1‑149 (MQ=255)
                                                                       gAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCatg                                                                               >  3:222009/1‑149 (MQ=255)
                                                                        aaGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCatga                                                                              <  2:7034612/149‑1 (MQ=255)
                                                                          gACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCAtt                                                                                                 >  2:2856535/1‑128 (MQ=255)
                                                                           aCTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGc                                                                           <  2:7231050/149‑1 (MQ=255)
                                                                            cTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGcc                                                                          <  2:595351/149‑1 (MQ=255)
                                                                             tGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCg                                                                         >  2:4017120/1‑149 (MQ=255)
                                                                               tCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGAt                                                                       >  4:2833914/1‑149 (MQ=255)
                                                                               tCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGAt                                                                       <  1:6068213/149‑1 (MQ=255)
                                                                                 aTAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCTGCATGATGCCGATAc                                                                     <  4:6824831/149‑1 (MQ=255)
                                                                                 aTAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgca                                                                                                               <  3:2644075/107‑1 (MQ=255)
                                                                                 aTAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgca                                                                                                               >  3:6537747/1‑107 (MQ=255)
                                                                                 aTAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCa                                                                                                   <  1:2919041/119‑1 (MQ=255)
                                                                                 aTAAATATTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCa                                                                                                   <  2:3517470/119‑1 (MQ=255)
                                                                                  tAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGTACTTCCAGCATGATGCCGATACg                                                                    >  1:3088515/1‑149 (MQ=255)
                                                                                        ttCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGttt                                                              >  3:5185055/1‑149 (MQ=255)
                                                                                         tCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTg                                                             <  1:6341807/149‑1 (MQ=255)
                                                                                         tCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTg                                                             <  2:4735582/149‑1 (MQ=255)
                                                                                         tCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTg                                                             <  1:5403011/149‑1 (MQ=255)
                                                                                           gCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCaa                                                                                                                                                   <  3:3555777/61‑1 (MQ=255)
                                                                                           gCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTggg                                                           >  4:2224874/1‑149 (MQ=255)
                                                                                            ccACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGa                                                          >  4:1764886/1‑149 (MQ=255)
                                                                                             cACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCATATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGaa                                                         <  4:3426995/149‑1 (MQ=255)
                                                                                             cACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGaa                                                         <  2:1648570/149‑1 (MQ=255)
                                                                                             cACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGaa                                                         <  2:1393999/149‑1 (MQ=255)
                                                                                              aCCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAt                                                        <  2:425398/149‑1 (MQ=255)
                                                                                              aCCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAt                                                        >  4:3997629/1‑149 (MQ=255)
                                                                                               cccGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAtt                                                       <  3:681219/149‑1 (MQ=255)
                                                                                               cccGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAtt                                                       <  4:4274645/149‑1 (MQ=255)
                                                                                                ccGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGCCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAttt                                                      >  1:1329827/1‑149 (MQ=255)
                                                                                                  gggTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCg                                                    >  3:5781/1‑149 (MQ=255)
                                                                                                       ttgttgCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAAcc                                               >  1:4315476/1‑149 (MQ=255)
                                                                                                         gttgCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGa                                             >  1:7079744/1‑149 (MQ=255)
                                                                                                          ttgCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGat                                            >  2:3335204/1‑149 (MQ=255)
                                                                                                           tgCGATCAACGGCCAGAATGTATTGAGTAAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTAGCCAGATGCGGCAGCATAAATACCATTGACGGCGCTGCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatc                                           <  4:6278684/149‑1 (MQ=255)
                                                                                                              gATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTa                                                  <  1:4996364/139‑1 (MQ=255)
                                                                                                              gATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatcatc                                        <  1:5881210/149‑1 (MQ=255)
                                                                                                                tCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCAtctc                                      >  2:5534865/1‑149 (MQ=255)
                                                                                                                   aCGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatc                                           <  3:4010249/141‑1 (MQ=255)
                                                                                                                      gCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGAc                                <  3:5468066/149‑1 (MQ=255)
                                                                                                                        cAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACtt                              >  4:5422161/1‑149 (MQ=255)
                                                                                                                         aGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTc                             <  3:3374070/149‑1 (MQ=255)
                                                                                                                            aTGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGGTGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatc                                           >  1:1539366/1‑132 (MQ=255)
                                                                                                                             tGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGt                         >  3:899750/1‑149 (MQ=255)
                                                                                                                               tATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATCCCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTcc                       <  2:3808752/149‑1 (MQ=255)
                                                                                                                               tATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTcc                       >  1:6805611/1‑149 (MQ=255)
                                                                                                                                aTTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCg                      >  3:3749218/1‑149 (MQ=255)
                                                                                                                                 ttGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCgg                     >  1:892864/1‑149 (MQ=255)
                                                                                                                                  tGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGc                    <  2:6371850/149‑1 (MQ=255)
                                                                                                                                   gAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCa                   <  4:1916494/149‑1 (MQ=255)
                                                                                                                                    aGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAc                  <  4:3622458/149‑1 (MQ=255)
                                                                                                                                      tCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGt                >  4:1468692/1‑149 (MQ=255)
                                                                                                                                        aGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTc              >  1:5680173/1‑149 (MQ=255)
                                                                                                                                        aGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTc              <  2:5050985/149‑1 (MQ=255)
                                                                                                                                         gATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTGCGGCACGTTCa             >  3:6182189/1‑149 (MQ=255)
                                                                                                                                           tCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGt                                                   <  4:2114121/109‑1 (MQ=255)
                                                                                                                                            cGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATc          <  1:6621234/149‑1 (MQ=255)
                                                                                                                                             gTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAt                                                        <  3:176252/102‑1 (MQ=255)
                                                                                                                                              ttGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAg        >  4:5008914/1‑149 (MQ=255)
                                                                                                                                              ttGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAg        >  2:3104898/1‑149 (MQ=255)
                                                                                                                                               tGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATAATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAgg       <  4:463625/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCG  >  NC_000913/2966434‑2966730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: