| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,222,725 | T→C | 17.7% | R31R (CGT→CGC) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,222,725 | 0 | T | C | 17.7% | 1942.6 / inf | 811 | R31R (CGT→CGC) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (339/328); new base C (69/75); total (408/403) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
Only 100 of 819 total aligned reads displayed.
TTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGG > NC_000913/3222577‑3222872 | ttACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGt > 1:6337633/1‑149 (MQ=255) cacaAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTAcc > 4:3267722/1‑149 (MQ=255) cacaAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTAcc > 1:6115564/1‑149 (MQ=255) cacaAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCAcc < 4:2777551/149‑1 (MQ=255) aaaCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGCGGCCCTATGAATCGCTGGGCAAACATTCTGCTCACCCACGAAACCCGTCTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGACAGTACCttt > 4:4857068/1‑149 (MQ=255) aaaCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCttt < 2:5534064/149‑1 (MQ=255) aaCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTg > 4:4617937/1‑149 (MQ=255) aCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGc < 1:4181994/149‑1 (MQ=255) cATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCcgcg > 3:2692878/1‑149 (MQ=255) cACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGaaa > 1:1712133/1‑149 (MQ=255) ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAAcc > 4:1051338/1‑149 (MQ=255) ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAAcc > 3:6456040/1‑149 (MQ=255) cATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACca < 4:1697148/149‑1 (MQ=255) aTGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGGAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATACTGTTGCCCAAGCGCGTACCTTTGCCCGCGAAACcag > 4:6892914/1‑149 (MQ=255) aTGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag > 4:572737/1‑149 (MQ=255) tGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcagc > 4:4278326/1‑149 (MQ=255) gAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAAc > 1:2391098/1‑144 (MQ=255) aaTGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcagcag > 1:7145886/1‑149 (MQ=255) ttCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGt > 4:6252517/1‑149 (MQ=255) ttCCGATTTCTCTCTACCGAGAGGCCCAATGAATGGCTGGGAAATCATTCAGCTCACGCACGACAACCGACTTGCGTCGCGTGCGCGCTTTTTTTCATATGATTCTGTTGCGCACGCGCCTACCTTTGCCCGCGAAGCCAGCCGCgtgt > 2:3316616/1‑149 (MQ=255) tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGtt < 1:853246/149‑1 (MQ=255) ccGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcagca > 4:2939163/1‑141 (MQ=255) ccGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGttt > 1:365282/1‑149 (MQ=255) tctctcTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGccc < 1:5110251/149‑1 (MQ=255) ctctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCt < 1:1858523/149‑1 (MQ=255) ctctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCt < 1:264185/149‑1 (MQ=255) ctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTa > 3:3837522/1‑149 (MQ=255) tctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTaa > 1:2985837/1‑149 (MQ=255) ctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAg > 3:5142884/1‑149 (MQ=255) tACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGc < 4:71346/149‑1 (MQ=255) aCCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCg < 4:5076156/149‑1 (MQ=255) gggAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGATAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTc < 3:6139757/149‑1 (MQ=255) gAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGt < 2:3653568/146‑1 (MQ=255) aGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGt < 2:2292638/149‑1 (MQ=255) gCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTgg < 1:385367/149‑1 (MQ=255) ccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGccc < 2:1294358/106‑1 (MQ=255) ccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGaa < 3:1087316/149‑1 (MQ=255) ccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGaa < 4:3657117/149‑1 (MQ=255) cTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAAt > 4:1910507/1‑149 (MQ=255) tATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAAtt < 1:4777860/149‑1 (MQ=255) tATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAAtt > 2:5842087/1‑149 (MQ=255) aaTCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCa < 3:4974857/149‑1 (MQ=255) cTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACtttt > 4:846608/1‑149 (MQ=255) cTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACtttt < 3:2432273/149‑1 (MQ=255) tGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACttttt > 3:2141059/1‑149 (MQ=255) gggAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACtttttt < 2:3942655/149‑1 (MQ=255) ggAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTg > 2:3987895/1‑149 (MQ=255) gAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGa > 2:7202312/1‑149 (MQ=255) gAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGa < 2:1869033/149‑1 (MQ=255) gAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGACTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGa > 4:1203329/1‑149 (MQ=255) aaaaCATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGAc < 2:6298137/149‑1 (MQ=255) aaaaCATTCAGCACACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGAc > 3:5461363/1‑149 (MQ=255) gCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGc > 3:2979001/1‑149 (MQ=255) ccACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTAc > 1:1053089/1‑149 (MQ=255) ccACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTAc > 3:3634805/1‑149 (MQ=255) ccACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTAc < 3:6619646/149‑1 (MQ=255) ccACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTAc < 2:2518225/149‑1 (MQ=255) cACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTAcc > 2:6153502/1‑149 (MQ=255) aCGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCa > 2:893666/1‑149 (MQ=255) aaaaCCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGaa < 2:2735655/149‑1 (MQ=255) aaaCCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAACCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAg > 2:5991569/1‑149 (MQ=255) aaCCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGCTTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATGCGCTGCAAGTACCAGAAGc < 2:6684260/149‑1 (MQ=255) aCCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGcc < 1:6280341/149‑1 (MQ=255) cGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCa < 4:3826252/113‑1 (MQ=255) gACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTc < 4:5421215/149‑1 (MQ=255) aCTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCa < 4:6580007/149‑1 (MQ=255) ccgcGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGa < 4:1337974/149‑1 (MQ=255) ccgcGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGa < 2:4580634/149‑1 (MQ=255) ccgcGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAGGTACCAGAAGCCTTCACCTCTGa < 1:6940248/149‑1 (MQ=255) gcgtgcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGt < 3:468086/149‑1 (MQ=255) gcgtgcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGt < 1:5899563/149‑1 (MQ=255) cgtgcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGtt < 3:828675/149‑1 (MQ=255) cgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTGTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATg < 1:447462/149‑1 (MQ=255) gtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTGCCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATgg < 2:1070212/149‑1 (MQ=255) gtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATgg > 2:4976878/1‑149 (MQ=255) aCTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCt < 4:2110191/149‑1 (MQ=255) cTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTg > 3:3307236/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 1:5440716/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 2:430177/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 3:1970428/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 4:2485177/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTCACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 4:5959508/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGaa > 1:2208408/1‑123 (MQ=255) ttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGAc < 1:1233152/149‑1 (MQ=255) ttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGAc < 2:2964646/149‑1 (MQ=255) tCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTgggg > 1:1471027/1‑149 (MQ=255) tatGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCa > 2:1501086/1‑149 (MQ=255) tatGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCa > 3:153371/1‑149 (MQ=255) tatGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCa > 2:6873174/1‑149 (MQ=255) tGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCata < 4:401883/149‑1 (MQ=255) tGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCata > 1:3442834/1‑149 (MQ=255) gATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCatat > 3:1842596/1‑149 (MQ=255) cTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTAcc < 3:4306823/149‑1 (MQ=255) tGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCg > 2:2102041/1‑149 (MQ=255) aaGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTc > 1:2786524/1‑104 (MQ=255) aaGCGCGCACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGcc > 3:6913492/1‑149 (MQ=255) cgcgTACCGTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCAtg < 2:1681998/149‑1 (MQ=255) gcgTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCAtgt < 1:6698803/149‑1 (MQ=255) gcgTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCAtgt < 3:1373285/149‑1 (MQ=255) gTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTgg > 3:5770206/1‑149 (MQ=255) | TTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGG > NC_000913/3222577‑3222872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |