Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,298,330 T→C 6.6% N173D (AAC→GAC)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,298,3300TC 6.6% 2272.0 / 96.9 712N173D (AAC→GAC) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base T (321/344);  new base C (25/22);  total (346/366)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 727 total aligned reads displayed.

TTACGCTCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGG  >  NC_000913/3298182‑3298478
                                                                                                                                                    |                                                                                                                                                    
    gCTCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCt                                                                                                                                                  <  2:3674878/149‑1 (MQ=255)
    gCTCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCt                                                                                                                                                  <  1:3131435/149‑1 (MQ=255)
    gCTCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCt                                                                                                                                                  >  4:38136/1‑149 (MQ=255)
     cTCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTCACCTg                                                                                                                                                 <  3:4094432/149‑1 (MQ=255)
      tCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTgg                                                                                                                                                >  4:6271395/1‑149 (MQ=255)
      tCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTgg                                                                                                                                                >  2:793846/1‑149 (MQ=255)
      tCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTgg                                                                                                                                                >  3:2452886/1‑149 (MQ=255)
       cACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGt                                                                                                                                               <  4:3609116/149‑1 (MQ=255)
        aCTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTa                                                                                                                                              >  2:1486360/1‑149 (MQ=255)
            tCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTAc                                                                                                                                                    <  2:6394273/139‑1 (MQ=255)
              aaTTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAAc                                                                                                                                        <  2:3201929/149‑1 (MQ=255)
               aTTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACg                                                                                                                                       <  1:798454/149‑1 (MQ=255)
               aTTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCAAGCATGACCCGCGCCACGTCTATCGCATCAATGGATTTCCAGTTACCTGGTAACAAACg                                                                                                                                       <  2:6667460/149‑1 (MQ=255)
                ttCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACgg                                                                                                                                      >  3:2802244/1‑149 (MQ=255)
                ttCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCCTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATAAACGGATTTCCAGTTACCTGGTAACAAACgg                                                                                                                                      <  4:4371227/149‑1 (MQ=255)
                   ttGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAAc                                                                                                                                   >  4:5379181/1‑149 (MQ=255)
                      ggAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAgc                                                                                                                                <  4:402321/149‑1 (MQ=255)
                       gAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAgcg                                                                                                                               >  1:2214974/1‑149 (MQ=255)
                       gAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAgcg                                                                                                                               >  1:5751458/1‑149 (MQ=255)
                        aataatTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAgcgg                                                                                                                              >  4:6830376/1‑149 (MQ=255)
                        aataatTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAgcgg                                                                                                                              >  1:120998/1‑149 (MQ=255)
                             ttattCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCaa                                                                                                                         >  3:6791838/1‑149 (MQ=255)
                               attCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCaaaa                                                                                                                       >  3:1007970/1‑149 (MQ=255)
                                  cAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGc                                                                                                                    <  2:1758687/149‑1 (MQ=255)
                                   aGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCg                                                                                                                   >  2:4445121/1‑149 (MQ=255)
                                    gCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGt                                                                                                                  >  3:1862952/1‑149 (MQ=255)
                                     ctctTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGtt                                                                                                                 <  2:1115764/149‑1 (MQ=255)
                                       ctTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTc                                                                                                               <  2:7099975/149‑1 (MQ=255)
                                        ttttGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCg                                                                                                              >  3:1625495/1‑149 (MQ=255)
                                         tttGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGt                                                                                                             <  1:6835481/149‑1 (MQ=255)
                                           tGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTc                                                                                                           <  4:2341814/149‑1 (MQ=255)
                                              gTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATc                                                                                                        >  1:2832718/1‑149 (MQ=255)
                                              gTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATc                                                                                                        >  3:3932021/1‑149 (MQ=255)
                                              gTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCCCAAAAAGCGTTTCGTTCATc                                                                                                        >  4:1116230/1‑149 (MQ=255)
                                               tAATGCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATcc                                                                                                       <  2:3436580/149‑1 (MQ=255)
                                                 aTTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGc                                                                                                     <  3:4829773/149‑1 (MQ=255)
                                                  ttCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCt                                                                                                    <  4:4716277/149‑1 (MQ=255)
                                                   tCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTg                                                                                                   >  1:5292179/1‑149 (MQ=255)
                                                    cTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGt                                                                                                  >  1:3583767/1‑149 (MQ=255)
                                                       aaGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTg                                                                                               <  3:657339/149‑1 (MQ=255)
                                                       aaGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTg                                                                                               <  2:1984369/149‑1 (MQ=255)
                                                          agCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTa                                                                                            <  1:505691/149‑1 (MQ=255)
                                                              tAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGAt                                                                                        <  1:7096678/149‑1 (MQ=255)
                                                                  aTCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGcc                                                                                    <  1:1687626/149‑1 (MQ=255)
                                                                  aTCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGcc                                                                                    <  2:4109985/149‑1 (MQ=255)
                                                                      tCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGt                                                                                >  3:6213943/1‑149 (MQ=255)
                                                                        aCGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTaa                                                                              <  4:5946488/149‑1 (MQ=255)
                                                                        aCGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTCACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTaa                                                                              <  1:4245984/149‑1 (MQ=255)
                                                                          gCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAAc                                                                                                                                            <  4:6126878/85‑1 (MQ=255)
                                                                          gCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACa                                                                            <  1:3281713/149‑1 (MQ=255)
                                                                          gCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACa                                                                            <  4:3106429/149‑1 (MQ=255)
                                                                          gCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACa                                                                            <  2:4819547/149‑1 (MQ=255)
                                                                             ttCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCg                                                                         >  3:3118716/1‑149 (MQ=255)
                                                                                 tGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACgg                                                                     <  3:6090944/149‑1 (MQ=255)
                                                                                  gCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGa                                                                                         >  4:5180734/1‑128 (MQ=255)
                                                                                  gCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCa                                                                                  >  2:1319818/1‑135 (MQ=255)
                                                                                   cTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTCACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGc                                                                   <  4:3303541/149‑1 (MQ=255)
                                                                                    tCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCg                                                                  >  1:4872460/1‑149 (MQ=255)
                                                                                    tCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCAGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCg                                                                  >  2:2693398/1‑149 (MQ=255)
                                                                                     cAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGa                                                                 <  4:2037137/149‑1 (MQ=255)
                                                                                        gCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCa                                                              >  4:150741/1‑149 (MQ=255)
                                                                                           gCATCGCCTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATg                                                           <  4:5728420/149‑1 (MQ=255)
                                                                                           gCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATg                                                           <  2:6876744/149‑1 (MQ=255)
                                                                                                   tctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAAttt                                                   >  1:6083215/1‑149 (MQ=255)
                                                                                                   tctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGAATTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAAttt                                                   >  3:996458/1‑149 (MQ=255)
                                                                                                    ctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTc                                                  <  2:5963113/149‑1 (MQ=255)
                                                                                                     tGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCg                                                 <  4:290407/149‑1 (MQ=255)
                                                                                                     tGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTCACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCg                                                 <  1:40487/149‑1 (MQ=255)
                                                                                                       ccAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGc                                               >  2:5458873/1‑149 (MQ=255)
                                                                                                       ccAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGc                                               >  1:5513748/1‑149 (MQ=255)
                                                                                                        cAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCACCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGcc                                              <  4:2603058/149‑1 (MQ=255)
                                                                                                         aGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCa                                             <  1:1447415/149‑1 (MQ=255)
                                                                                                            aTGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGtt                                          >  2:507850/1‑149 (MQ=255)
                                                                                                              gACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCa                                                                                  >  3:1333258/1‑107 (MQ=255)
                                                                                                              gACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCt                                        <  3:3610071/149‑1 (MQ=255)
                                                                                                                cccGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTaa                                                                              <  2:5611491/109‑1 (MQ=255)
                                                                                                                cccGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTgg                                      >  3:6372211/1‑149 (MQ=255)
                                                                                                                 ccGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTggg                                     >  2:3842402/1‑149 (MQ=255)
                                                                                                                     gcCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGAt                                 <  1:4445121/149‑1 (MQ=255)
                                                                                                                      ccACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGAtt                                <  3:4932469/149‑1 (MQ=255)
                                                                                                                      ccACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGAtt                                >  2:2442144/1‑149 (MQ=255)
                                                                                                                       cACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGtt                                                                                                                 <  4:1747212/67‑1 (MQ=255)
                                                                                                                          gTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATg                            >  1:2455269/1‑149 (MQ=255)
                                                                                                                             tctcGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACAt                                                                           <  4:5020447/99‑1 (MQ=255)
                                                                                                                               tcGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTg                                       <  3:5531735/133‑1 (MQ=255)
                                                                                                                                 gCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCttcttc                     <  1:1000935/149‑1 (MQ=255)
                                                                                                                                 gCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCttcttc                     <  1:3810289/149‑1 (MQ=255)
                                                                                                                                       aTGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCAtctc               >  3:4343429/1‑149 (MQ=255)
                                                                                                                                        tGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTcc              >  4:7028198/1‑149 (MQ=255)
                                                                                                                                         ggATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTccc             <  1:6184708/149‑1 (MQ=255)
                                                                                                                                         ggATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTccc             <  4:6544324/149‑1 (MQ=255)
                                                                                                                                           aTTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCt           >  2:4263031/1‑149 (MQ=255)
                                                                                                                                            tttCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCtt          >  2:6109953/1‑149 (MQ=255)
                                                                                                                                            tttCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCtt          >  2:2147202/1‑149 (MQ=255)
                                                                                                                                            tttCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCtt          <  3:5728527/149‑1 (MQ=255)
                                                                                                                                             ttCCAGTTACCTGGTAACAAACGGAACAGCGGCGAAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCttt         >  1:6379313/1‑149 (MQ=255)
                                                                                                                                                 aGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAAttt                                                   <  3:3798155/103‑1 (MQ=255)
                                                                                                                                                  gTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGAcgc    >  3:5252846/1‑149 (MQ=255)
                                                                                                                                                    tACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCgg  <  3:2101379/149‑1 (MQ=255)
                                                                                                                                                    tACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCgg  <  3:3276399/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTACGCTCACTATCAATTCTTGGGAATAATTATTCAGCTCTTTTGCGTAATTCTGAAGAGCTTAAAATCGTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGG  >  NC_000913/3298182‑3298478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: