Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,418,440 T→C 15.8% C17C (TGT→TGC yhdV → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,4400TC15.8% 1972.5 / 300.6 773C17C (TGT→TGCyhdVputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (300/350);  new base C (57/66);  total (357/416)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 787 total aligned reads displayed.

TCCGCAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGG  >  NC_000913/3418292‑3418588
                                                                                                                                                    |                                                                                                                                                    
tcCGCAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCtgt                                                                                                                                                      <  2:6004879/149‑1 (MQ=255)
  cGCAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCACATTGCTGGCGGGCTGTGc                                                                                                                                                    >  4:4862987/1‑149 (MQ=255)
   gCAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCt                                                                                                                                                   >  1:1800130/1‑149 (MQ=255)
   gCAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCt                                                                                                                                                   >  2:1714765/1‑149 (MQ=255)
    cAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTc                                                                                                                                                  <  3:4247747/149‑1 (MQ=255)
    cAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTc                                                                                                                                                  <  2:744568/149‑1 (MQ=255)
     aaGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCa                                                                                                                                                 >  1:5213976/1‑149 (MQ=255)
             taACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGc                                                                                                                                         <  4:2789588/149‑1 (MQ=255)
             taACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGc                                                                                                                                         >  2:2258815/1‑149 (MQ=255)
                 cTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCTTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTg                                                                                                                                     >  3:2456509/1‑149 (MQ=255)
                     ggTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGtt                                                                                                                                 <  2:4777089/149‑1 (MQ=255)
                       tGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTcc                                                                                                                               >  2:2692058/1‑149 (MQ=255)
                         aTAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCgg                                                                                                                             <  1:128932/149‑1 (MQ=255)
                         aTAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCgg                                                                                                                             <  2:7097919/149‑1 (MQ=255)
                         aTAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCgg                                                                                                                             <  2:6470030/149‑1 (MQ=255)
                         aTAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCgg                                                                                                                             >  1:5356799/1‑149 (MQ=255)
                          tAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGt                                                                                                                            >  1:4217788/1‑149 (MQ=255)
                            aaaTGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGttt                                                                                                                          <  4:2377617/149‑1 (MQ=255)
                              aTGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTAt                                                                                                                        <  3:3891763/149‑1 (MQ=255)
                              aTGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTAt                                                                                                                        <  3:5648373/149‑1 (MQ=255)
                              aTGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTAt                                                                                                                        <  4:1538955/149‑1 (MQ=255)
                              aTGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTAt                                                                                                                        <  4:2957601/149‑1 (MQ=255)
                               tGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATg                                                                                                                       >  1:2134032/1‑149 (MQ=255)
                                ggCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGa                                                                                                                      <  2:4283967/149‑1 (MQ=255)
                                ggCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGa                                                                                                                      <  3:6456985/149‑1 (MQ=255)
                                ggCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGa                                                                                                                      <  1:7022929/149‑1 (MQ=255)
                                 gCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGAc                                                                                                                     <  4:5269909/149‑1 (MQ=255)
                                      cAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCa                                                                                                                <  3:592327/149‑1 (MQ=255)
                                       aGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAg                                                                                                               <  2:5218020/149‑1 (MQ=255)
                                       aGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAg                                                                                                               <  3:116859/149‑1 (MQ=255)
                                        gCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAgg                                                                                                              <  3:3123306/149‑1 (MQ=255)
                                         cTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAggg                                                                                                             >  1:785372/1‑149 (MQ=255)
                                           cGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGgtc                                                                                                           <  1:3894258/149‑1 (MQ=255)
                                           cGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGgtc                                                                                                           <  4:6536092/149‑1 (MQ=255)
                                            gTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGgtcg                                                                                                          >  4:6352671/1‑149 (MQ=255)
                                             ttAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGgtcgt                                                                                                         >  3:46303/1‑149 (MQ=255)
                                               aataaGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCt                                                                                                       >  4:5677385/1‑149 (MQ=255)
                                               aataaGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCt                                                                                                       <  3:1943970/149‑1 (MQ=255)
                                                ataaGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTg                                                                                                      >  4:758144/1‑149 (MQ=255)
                                                ataaGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTg                                                                                                      <  1:6568202/149‑1 (MQ=255)
                                                ataaGAGAGTTAACCTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTg                                                                                                      >  2:3747696/1‑149 (MQ=255)
                                                   agagagTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGtt                                                                                                   <  1:4064126/149‑1 (MQ=255)
                                                   agagagTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGtt                                                                                                   >  1:3742185/1‑149 (MQ=255)
                                                    gagagTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTc                                                                                                  <  3:4915411/149‑1 (MQ=255)
                                                        gTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCacac                                                                                              >  1:5017509/1‑149 (MQ=255)
                                                         ttAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTg                                                                                                      <  3:8468/140‑1 (MQ=255)
                                                             cTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCCCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGgtcgtc                                                                                                        >  3:2761091/1‑134 (MQ=255)
                                                              ttattaAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATAc                                                                                        <  1:3498939/149‑1 (MQ=255)
                                                              ttattaAGCGTTAGCGTTTATGACTGAGGTAACACCATGAAATGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGGGGGCTGCGCGCAGGATTCGCCCTGCGATCCGGTTTATGACGATCAGGGTCGTCCGGTTCACACCACTAc                                                                                        <  4:6071417/149‑1 (MQ=255)
                                                                attaAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCt                                                                                      >  4:7011300/1‑149 (MQ=255)
                                                                attaAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCt                                                                                      <  2:2011590/149‑1 (MQ=255)
                                                                 ttaAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTg                                                                                     >  3:667980/1‑149 (MQ=255)
                                                                  taAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTCCACACCAATACCTGt                                                                                    >  3:1408419/1‑149 (MQ=255)
                                                                       gttagcgttTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGaa                                                                               >  4:2559843/1‑149 (MQ=255)
                                                                             gttTATTACTGAGGTAACACCATGAAAAGATAAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCAc                                                                         <  4:1394434/149‑1 (MQ=255)
                                                                              tttATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGgtcgtc                                                                                                        <  1:4799202/117‑1 (MQ=255)
                                                                              tttATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACGAATACCTGTATGAAAGGCACg                                                                        >  4:6712111/1‑149 (MQ=255)
                                                                                 attaCTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACt                                                                     <  2:6205467/149‑1 (MQ=255)
                                                                                      tGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGaaa                                                                              <  3:5346297/135‑1 (MQ=255)
                                                                                      tGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGaaa                                                                              >  2:3444654/1‑135 (MQ=255)
                                                                                        aGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATa                                                              >  2:2381383/1‑149 (MQ=255)
                                                                                         ggTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATaa                                                             >  4:3467654/1‑149 (MQ=255)
                                                                                            aaCACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATAc                                                                                        <  1:922798/119‑1 (MQ=255)
                                                                                            aaCACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTg                                                          <  1:2366202/149‑1 (MQ=255)
                                                                                             acacCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTgg                                                         <  3:2159090/149‑1 (MQ=255)
                                                                                             acacCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTgg                                                         <  4:3270882/149‑1 (MQ=255)
                                                                                             acacCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTgg                                                         >  2:5362063/1‑149 (MQ=255)
                                                                                               acCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGa                                                       >  3:7009163/1‑149 (MQ=255)
                                                                                                 cATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCt                                                                                                                                                   >  2:4496744/1‑55 (MQ=255)
                                                                                                  aTGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGa                                                                                                                      <  2:496347/83‑1 (MQ=255)
                                                                                                  aTGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTg                                                                                     <  2:1486105/116‑1 (MQ=255)
                                                                                                  aTGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAAc                                                    >  1:1003951/1‑149 (MQ=255)
                                                                                                      aaaGATTAATGCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTATATGACGATCAGGGTCGTCTGGTTCACACCAAGAGCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCt                                                >  4:4635301/1‑149 (MQ=255)
                                                                                                       aaGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGCTTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAGGGTACGACTCAGGATAACTGGGAAACGGCTg                                               <  4:4372163/149‑1 (MQ=255)
                                                                                                       aaGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTg                                               <  4:392978/149‑1 (MQ=255)
                                                                                                       aaGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTg                                               >  1:6725824/1‑149 (MQ=255)
                                                                                                        aGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTgg                                              <  3:3454547/149‑1 (MQ=255)
                                                                                                        aGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTgg                                              <  3:2930304/149‑1 (MQ=255)
                                                                                                        aGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTgg                                              >  4:364837/1‑149 (MQ=255)
                                                                                                         gATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTgg                                              >  2:958150/1‑148 (MQ=255)
                                                                                                          aTTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTgggg                                            >  1:5945830/1‑149 (MQ=255)
                                                                                                           ttAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGc                                           >  1:3220104/1‑149 (MQ=255)
                                                                                                               ttCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTAtt                                       <  1:6396781/149‑1 (MQ=255)
                                                                                                                   tgtgGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCcg                                   <  2:6772746/149‑1 (MQ=255)
                                                                                                                   tgtgGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCcg                                   >  4:5489985/1‑149 (MQ=255)
                                                                                                                   tgtgGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCcg                                   >  2:4142278/1‑149 (MQ=255)
                                                                                                                       gCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCcggcgg                               >  2:1174902/1‑149 (MQ=255)
                                                                                                                         aTTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCgggg                             >  2:3254942/1‑149 (MQ=255)
                                                                                                                              tCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgct                        >  3:6113664/1‑149 (MQ=255)
                                                                                                                               cACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgctg                       >  1:4509028/1‑149 (MQ=255)
                                                                                                                                aCCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgctgc                      >  4:1629762/1‑149 (MQ=255)
                                                                                                                                aCCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgctgc                      >  1:2688054/1‑149 (MQ=255)
                                                                                                                                 ccGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAgctgct                     >  4:1071732/1‑149 (MQ=255)
                                                                                                                                    cATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGtt                  <  3:1712178/149‑1 (MQ=255)
                                                                                                                                    cATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGtt                  <  3:3697415/149‑1 (MQ=255)
                                                                                                                                      ttGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGc                >  2:3945972/1‑149 (MQ=255)
                                                                                                                                          tGGCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTgga            <  4:2018287/149‑2 (MQ=255)
                                                                                                                                           ggCGGGCTGCGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGcc           <  2:2983864/149‑1 (MQ=255)
                                                                                                                                            gCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCt          <  2:5681697/149‑1 (MQ=255)
                                                                                                                                            gCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCt          <  1:1650447/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCGCAAGATTTATAACCTCCGGTGATAAAATGGCATTCAGCTCGTTAATAAGAGAGTTAACTTATTAAGCGTTAGCGTTTATTACTGAGGTAACACCATGAAAAGATTAATTCCTGTGGCATTGCTCACCGCATTGCTGGCGGGCTGTGCTCACGATTCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGG  >  NC_000913/3418292‑3418588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: