Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,441,571 T→C 15.7% E35E (GAA→GAG rpsD ← 30S ribosomal subunit protein S4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,441,5710TC15.7% 1965.5 / 304.3 770E35E (GAA→GAGrpsD30S ribosomal subunit protein S4
Reads supporting (aligned to +/- strand):  ref base T (309/340);  new base C (55/66);  total (364/406)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 808 total aligned reads displayed.

TGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGAC  >  NC_000913/3441423‑3441719
                                                                                                                                                    |                                                                                                                                                    
tGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGt                                                                                                                                                      <  1:2356893/149‑1 (MQ=255)
   cTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCa                                                                                                                                                   >  1:5366866/1‑149 (MQ=255)
    tttCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCaa                                                                                                                                                  >  1:3906270/1‑149 (MQ=255)
    tttCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCaa                                                                                                                                                  >  3:3050605/1‑149 (MQ=255)
     ttCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGtt                                                                                                                                                     <  1:5633977/145‑1 (MQ=255)
     ttCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGtt                                                                                                                                                     >  2:5633977/1‑145 (MQ=255)
        aGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAAtttt                                                                                                                                              <  2:653324/149‑1 (MQ=255)
         gACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTa                                                                                                                                             >  3:6795041/1‑149 (MQ=255)
         gACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTa                                                                                                                                             >  1:5053330/1‑149 (MQ=255)
            gTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAAt                                                                                                                                                 >  3:4937132/1‑142 (MQ=255)
            gTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTacac                                                                                                                                          >  1:3311037/1‑149 (MQ=255)
             tgctgcTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACt                                                                                                                                         <  4:1361274/149‑1 (MQ=255)
              gctgctTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACtt                                                                                                                                        <  1:728598/149‑1 (MQ=255)
                tgctTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTgg                                                                                                                                      >  2:3316603/1‑149 (MQ=255)
                tgctTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTgg                                                                                                                                      >  2:2974662/1‑149 (MQ=255)
                tgctTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTgg                                                                                                                                      >  2:4202911/1‑149 (MQ=255)
                 gctTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGt                                                                                                                                     >  2:1523904/1‑149 (MQ=255)
                 gctTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGt                                                                                                                                     >  2:4996495/1‑149 (MQ=255)
                    tcttTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTatc                                                                                                                                  <  1:1145417/149‑1 (MQ=255)
                      tttGTAGTAGTTACGGAACTGGCGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTatcga                                                                                                                                >  2:6292977/1‑149 (MQ=255)
                       ttGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTatcgat                                                                                                                               <  2:1315247/149‑1 (MQ=255)
                         gtagtaGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGCACTTTTTGCTTTTCACGCAACTGCACACAATAGTCAGACAGACGCGGTTTACGCGCACCGTCCTGCCCAGGAGCTTGTTCAATTTTACACTTGGTatcgatcg                                                                                                                             >  2:5371565/1‑149 (MQ=255)
                         gtagtaGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTatcgatcg                                                                                                                             >  1:1550362/1‑149 (MQ=255)
                               gtTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGaa                                                                                                                       >  1:668118/1‑149 (MQ=255)
                               gtTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGaa                                                                                                                       <  4:2089371/149‑1 (MQ=255)
                                ttACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATcgc                                                                                                                            <  2:1062781/143‑1 (MQ=255)
                                ttACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAAc                                                                                                                      >  1:2933532/1‑149 (MQ=255)
                                  aCGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGc                                                                                                                    >  3:5401655/1‑149 (MQ=255)
                                  aCGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGc                                                                                                                    >  3:5759675/1‑149 (MQ=255)
                                    ggAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCa                                                                                                                  <  3:6388247/149‑1 (MQ=255)
                                    ggAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTatcgatc                                                                                                                              >  2:4351057/1‑137 (MQ=255)
                                       aCTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGAc                                                                                                               >  1:4303027/1‑149 (MQ=255)
                                         tGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACtt                                                                                                             >  1:4879806/1‑149 (MQ=255)
                                           aCGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTaa                                                                                                           <  4:4110173/149‑1 (MQ=255)
                                           aCGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTaa                                                                                                           <  3:3622837/149‑1 (MQ=255)
                                           aCGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTaa                                                                                                           <  4:4299590/149‑1 (MQ=255)
                                           aCGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTaa                                                                                                           >  4:5914021/1‑149 (MQ=255)
                                           aCGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTaa                                                                                                           <  4:1115953/149‑1 (MQ=255)
                                              cTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATcgcgc                                                                                                                          >  4:1602939/1‑131 (MQ=255)
                                                ccAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAAt                                                                                                      <  2:3906270/149‑1 (MQ=255)
                                                ccAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAAt                                                                                                      <  3:1223353/149‑1 (MQ=255)
                                                       atcATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTATGGAATAAGTCgg                                                                                               >  2:1566286/3‑149 (MQ=255)
                                                       acCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCgg                                                                                               >  4:5511804/1‑149 (MQ=255)
                                                       acCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCgg                                                                                               >  4:3159120/1‑149 (MQ=255)
                                                        ccATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAAtttt                                                                                                                                              <  3:5530156/101‑1 (MQ=255)
                                                        ccATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTaca                                                                                                                                           <  1:2009517/104‑1 (MQ=255)
                                                        ccATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGt                                                                                              <  3:1678793/149‑1 (MQ=255)
                                                            aGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTaca                                                                                                                                           >  2:6767469/1‑100 (MQ=255)
                                                             gATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCt                                                                                         <  3:5248702/149‑1 (MQ=255)
                                                                  ggCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTTGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCacga                                                                                    >  3:1046703/1‑149 (MQ=255)
                                                                     gAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAAt                                                                                                                                                 >  3:815693/1‑85 (MQ=255)
                                                                      aaCTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGc                                                                                <  3:3439618/149‑1 (MQ=255)
                                                                      aaCTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGc                                                                                >  2:2284897/1‑149 (MQ=255)
                                                                         tttttGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAATTCGGTGCCCTCACGACGGCTCa                                                                             >  3:4371766/1‑149 (MQ=255)
                                                                         tttttGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCa                                                                             <  3:3191336/149‑1 (MQ=255)
                                                                         tttttGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCa                                                                             >  2:843568/1‑149 (MQ=255)
                                                                               cTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGa                                                                       <  4:5039401/149‑1 (MQ=255)
                                                                               cTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGa                                                                       <  4:6233560/149‑1 (MQ=255)
                                                                               cTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGa                                                                       <  2:4877445/149‑1 (MQ=255)
                                                                                ttttCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAg                                                                      >  1:6134309/1‑149 (MQ=255)
                                                                                ttttCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAg                                                                      >  4:3483275/1‑149 (MQ=255)
                                                                                 tttCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGc                                                                     <  2:3434242/149‑1 (MQ=255)
                                                                                    cACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTa                                                                  <  4:6603906/149‑1 (MQ=255)
                                                                                      cGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAgg                                                                <  2:3231584/149‑1 (MQ=255)
                                                                                       gCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGa                                                               <  2:2088096/149‑1 (MQ=255)
                                                                                       gCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGa                                                               >  1:3638190/1‑149 (MQ=255)
                                                                                                   cATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCtt                                                   <  1:1714623/149‑1 (MQ=255)
                                                                                                   cATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCtt                                                   >  4:5324547/1‑149 (MQ=255)
                                                                                                    aTAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTg                                                  <  3:4149382/149‑1 (MQ=255)
                                                                                                          agacagacGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACTACGGCTCAGCTTGAGCTTCGGACCCAAATATCTTGCCAttt                                            <  2:182254/149‑1 (MQ=255)
                                                                                                          agacagacGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCAttt                                            >  4:6848101/1‑149 (MQ=255)
                                                                                                           gacagacGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGc                                                 <  1:5941497/143‑1 (MQ=255)
                                                                                                           gacagacGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCAtttt                                           >  2:4561670/1‑149 (MQ=255)
                                                                                                              agacGCGGTTTACGCGCACCGTGCTGGCCAGGAGCCTGCTCAATTTTACACTTgg                                                                                                                                      <  4:4491090/55‑1 (MQ=255)
                                                                                                                acGCGGTCTACGCGCACCGTGCTGGCCAGGAGCTTGTCCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTGGAGCTTAGCACCCAAATATCTTGCCATtttctttc                                      >  4:490809/1‑149 (MQ=255)
                                                                                                                  gcgGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTtctc                                    <  3:135105/149‑1 (MQ=255)
                                                                                                                      tttACGCGCACCGTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCcaac                                >  1:2850496/1‑149 (MQ=255)
                                                                                                                         aCGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACaaa                             <  1:705801/149‑1 (MQ=255)
                                                                                                                           gcgcACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATtttctttc                                      <  2:1697482/138‑1 (MQ=255)
                                                                                                                            cgcACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCt                          <  4:122794/149‑1 (MQ=255)
                                                                                                                            cgcACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCt                          <  1:5809521/149‑1 (MQ=255)
                                                                                                                             gcACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTg                         <  3:217779/149‑1 (MQ=255)
                                                                                                                                  gTGCTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGaaaa                    <  1:2594827/149‑1 (MQ=255)
                                                                                                                                     cTGGCCAGGAGCTTGTTCAATTTTTCACTTCGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGa                 <  3:2397829/149‑1 (MQ=255)
                                                                                                                                     cTGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGa                 <  1:4561670/149‑1 (MQ=255)
                                                                                                                                      tGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAg                <  1:404286/149‑1 (MQ=255)
                                                                                                                                      tGGCCAGGAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAg                <  4:2430558/149‑1 (MQ=255)
                                                                                                                                        gCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGc              >  4:7047133/1‑149 (MQ=255)
                                                                                                                                        gCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTACCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGc              <  1:1032266/149‑1 (MQ=255)
                                                                                                                                         ccAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCt                          >  4:2707183/1‑136 (MQ=255)
                                                                                                                                         ccAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCt                          <  3:2707183/136‑1 (MQ=255)
                                                                                                                                          cAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAg                <  2:6062754/145‑1 (MQ=255)
                                                                                                                                          cAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGt            >  4:3843669/1‑149 (MQ=255)
                                                                                                                                          cAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGt            >  4:5729398/1‑149 (MQ=255)
                                                                                                                                           aGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGtt           <  2:7120565/149‑1 (MQ=255)
                                                                                                                                            ggAGCTTGCTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTta          <  2:5883807/149‑1 (MQ=255)
                                                                                                                                             gAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTtat         >  3:3914812/1‑149 (MQ=255)
                                                                                                                                               gCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATAc       >  4:1862721/1‑149 (MQ=255)
                                                                                                                                                cTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATAcg      >  3:4726585/1‑149 (MQ=255)
                                                                                                                                                    ttCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGa                                                                       <  2:2706762/80‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGGCCAGGAGCTTGTTCAATTTTACACTTGGTATCGATCGCGCGAACGCCAGACTTAAGGAATAAGTCGGTGCCCTCACGACGGCTCAGCTTGAGCTTAGGACCCAAATATCTTGCCATTTTCTTTCTCCAACAAACCTGGAAAACGAGGCGTTATACGCGAC  >  NC_000913/3441423‑3441719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: