Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,447,561 G→A 13.1% N69N (AAC→AAT rplX ← 50S ribosomal subunit protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,447,5610GA13.1% 2244.4 / 254.6 813N69N (AAC→AATrplX50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base G (336/370);  new base A (48/59);  total (384/429)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 822 total aligned reads displayed.

TGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTAC  >  NC_000913/3447414‑3447709
                                                                                                                                                   |                                                                                                                                                    
tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGt                                                                                                                                                     <  3:239798/149‑1 (MQ=255)
 gtagtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGtt                                                                                                                                                    <  1:4952762/149‑1 (MQ=255)
  tagtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTATGTTg                                                                                                                                                   >  4:4455846/1‑149 (MQ=255)
   agtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTgg                                                                                                                                                  <  3:5541988/149‑1 (MQ=255)
     taATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaa                                                                                                                                                >  1:3396090/1‑149 (MQ=255)
      aaTCATGCAGTTTCGCCATCGTACTACTCCATATTACTTGTTAGTTTCGCTGTTAGACTTGAAGAAACGGACTATTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaaa                                                                                                                                               <  2:1769653/149‑1 (MQ=255)
         cATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCt                                                                                                                                            <  2:7233395/149‑1 (MQ=255)
            gCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGaa                                                                                                                                         >  2:4400482/1‑149 (MQ=255)
             cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGCATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCt                                                                                                                                            <  4:4875097/145‑1 (MQ=255)
                 ttCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCGTTGAAGATTGCTACGTTGGAAACCTGAATAGCg                                                                                                                                    <  3:4518129/149‑1 (MQ=255)
                    gCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAc                                                                                                                                              <  3:6640306/136‑1 (MQ=255)
                      cATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAcc                                                                                                                                             <  1:3086899/135‑1 (MQ=255)
                      cATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttc                                                                                                                               >  4:2014543/1‑149 (MQ=255)
                       aTCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttct                                                                                                                              >  4:1153575/1‑149 (MQ=255)
                       aTCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttct                                                                                                                              >  3:5283833/1‑149 (MQ=255)
                         cGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCttt                                                                                                                            <  3:2903947/149‑1 (MQ=255)
                         cGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCttt                                                                                                                            <  1:2830857/149‑1 (MQ=255)
                            actactCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                                                                                                                         <  1:1027957/149‑1 (MQ=255)
                            actactCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                                                                                                                         <  1:6608644/149‑1 (MQ=255)
                            actactCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                                                                                                                         >  4:516642/1‑149 (MQ=255)
                              tactCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCaa                                                                                                                       >  2:4874770/1‑149 (MQ=255)
                               actCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAAc                                                                                                                      <  1:5376225/149‑1 (MQ=255)
                                ctCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGa                                                                                                                                          <  1:1375962/128‑1 (MQ=255)
                                ctCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACg                                                                                                                     >  3:3733364/1‑149 (MQ=255)
                                ctCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTg                                                                                                                                           <  2:4689404/127‑1 (MQ=255)
                                 tCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGa                                                                                                                    >  3:606725/1‑149 (MQ=255)
                                 tCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGa                                                                                                                    >  3:2778970/1‑149 (MQ=255)
                                   cAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCCTTTTCAACGATg                                                                                                                  >  3:3532942/1‑149 (MQ=255)
                                   cAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTAAGCCTTGCTGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATg                                                                                                                  >  3:1106914/1‑149 (MQ=255)
                                   cAAATTACTTGATAGTTTCGCTATTAGACTTGAAGAAACGGACTTTTTTACTGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGCAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATg                                                                                                                  >  4:5993657/1‑149 (MQ=255)
                                         aCTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAccc                                                                                                            <  2:2233264/149‑1 (MQ=255)
                                             gATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggtt                                                                                                        >  4:3543554/1‑149 (MQ=255)
                                              aTAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg                                                                                                       <  1:77041/149‑1 (MQ=255)
                                              aTAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg                                                                                                       <  4:3708199/149‑1 (MQ=255)
                                                  tttCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTc                                                                                                   >  1:2059822/1‑149 (MQ=255)
                                                   ttCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCa                                                                                                  >  2:1224037/1‑149 (MQ=255)
                                                                ttGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGcc                                                                                                                <  3:2021108/122‑1 (MQ=255)
                                                                ttGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCgg                                                                                     >  2:4229801/1‑149 (MQ=255)
                                                                ttGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCgg                                                                                     <  1:1866606/149‑1 (MQ=255)
                                                                ttGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCgg                                                                                     >  3:3195780/1‑149 (MQ=255)
                                                                   aagaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCtt                                                                                  >  2:5521089/1‑149 (MQ=255)
                                                                    agaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGcc                                                                                             >  1:5539644/1‑137 (MQ=255)
                                                                    agaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTc                                                                                 <  3:943779/149‑1 (MQ=255)
                                                                          ggACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGt                                                                           >  3:1307918/1‑149 (MQ=255)
                                                                          ggACCTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGt                                                                           >  1:3828339/1‑149 (MQ=255)
                                                                           gACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGtt                                                                          >  1:362506/1‑149 (MQ=255)
                                                                            aCTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGttt                                                                         <  1:6998810/149‑1 (MQ=255)
                                                                             cTTTTTTACCGTCTTCGAATCTAAAGTCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                        <  1:4096155/149‑1 (MQ=255)
                                                                              ttttttACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAAt                                                                                                                                        <  3:1642905/84‑1 (MQ=255)
                                                                              ttttttACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                        <  4:1485822/148‑1 (MQ=255)
                                                                              ttttttACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                        >  3:1485822/1‑148 (MQ=255)
                                                                               tttttACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCtt                                                                      <  1:4489511/149‑1 (MQ=255)
                                                                                ttttACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTa                                                                     <  3:2572391/149‑1 (MQ=255)
                                                                                 tttACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTaa                                                                    <  1:1672442/149‑1 (MQ=255)
                                                                                   tACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttc                                                                                                                               <  3:6957207/88‑1 (MQ=255)
                                                                                    aCCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCa                                                                 <  1:5678664/149‑1 (MQ=255)
                                                                                    aCCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCa                                                                 >  1:4729059/1‑149 (MQ=255)
                                                                                    aCCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCa                                                                 <  1:2414325/149‑1 (MQ=255)
                                                                                           tCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGAt                                                          <  2:6203462/149‑1 (MQ=255)
                                                                                             gAATCTAAAGCCTACACGGTCAGCGTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCACCGATGCCACCCGGTTGGTACAGGGCCGGAACCGGGTTCTGATGTTTCTTAACCAGGTTGAAAc                                                        <  4:6206767/149‑1 (MQ=255)
                                                                                             gAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATAc                                                        <  4:6680464/149‑1 (MQ=255)
                                                                                               aTCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAg                                                                <  2:6070046/139‑1 (MQ=255)
                                                                                               aTCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGAt                                                          >  2:5649995/1‑145 (MQ=255)
                                                                                                 cTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTc                                                    >  2:2717543/1‑149 (MQ=255)
                                                                                                       ccTACACGGTCATCCTTGCCGGTTGCTGCATTGAAGATTGCTACGTTGGGAACCTGAATAGCGTCTTCTTTTTCAGCGATGCCTCCCGGTTAGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAAt                                              <  2:1470659/149‑1 (MQ=255)
                                                                                                       ccTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAAt                                              <  2:3937374/149‑1 (MQ=255)
                                                                                                        cTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATg                                             <  2:6458775/149‑1 (MQ=255)
                                                                                                         tACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGa                                            <  3:4260487/149‑1 (MQ=255)
                                                                                                           cacGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                        <  2:3199343/119‑1 (MQ=255)
                                                                                                            acGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCt                                         >  4:763107/1‑149 (MQ=255)
                                                                                                               gTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGc                                      <  2:3005046/149‑1 (MQ=255)
                                                                                                                tCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGcc                                     >  4:4189178/1‑149 (MQ=255)
                                                                                                                  aGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAAt                                              >  4:3281679/1‑138 (MQ=255)
                                                                                                                   gCCTTGCCGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGCTGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGa                                  <  2:3377605/149‑1 (MQ=255)
                                                                                                                    ccTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAggg                                                                                               <  3:4760800/87‑1 (MQ=255)
                                                                                                                    ccTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGaa                                 <  1:604059/149‑1 (MQ=255)
                                                                                                                    ccTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGaa                                 <  2:2502375/149‑1 (MQ=255)
                                                                                                                    ccTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGaa                                 <  4:2872235/149‑1 (MQ=255)
                                                                                                                        gCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACa                             >  4:1345218/1‑149 (MQ=255)
                                                                                                                         ccGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCtt                                        <  4:5700227/137‑1 (MQ=255)
                                                                                                                         ccGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGtt                                                             <  4:2550338/116‑1 (MQ=255)
                                                                                                                          cGGTTGCCGCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAgg                           >  2:5157034/1‑149 (MQ=255)
                                                                                                                           ggTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGa                                                                                                                    >  3:407699/1‑59 (MQ=255)
                                                                                                                           ggTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGa                          <  2:6931124/149‑1 (MQ=255)
                                                                                                                             ttGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACa                        >  2:2076324/1‑149 (MQ=255)
                                                                                                                                ccGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTc                     <  3:1808426/149‑1 (MQ=255)
                                                                                                                                  gCATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCtt                   >  3:5726560/1‑149 (MQ=255)
                                                                                                                                   cATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                        <  4:2230974/95‑1 (MQ=255)
                                                                                                                                   cATTGAAGATTGCTACATTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGttt                                                                         >  1:626869/1‑94 (MQ=255)
                                                                                                                                    aTTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTaa                 <  1:6457346/149‑1 (MQ=255)
                                                                                                                                     ttGAAGATTGCTACATTGGAAACCTGAATCGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAAc                >  4:2736711/1‑149 (MQ=255)
                                                                                                                                         aGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTa            >  3:621419/1‑149 (MQ=255)
                                                                                                                                          gATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTAc           >  3:5004476/1‑149 (MQ=255)
                                                                                                                                             tGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACcgc        >  4:7022448/1‑149 (MQ=255)
                                                                                                                                               cTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttggtt                                                                                                    >  4:5427949/1‑55 (MQ=255)
                                                                                                                                                tACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTcaa                                                  >  2:3624076/1‑104 (MQ=255)
                                                                                                                                                caCGTTGGAAACCCGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGtt     >  4:4614188/2‑149 (MQ=255)
                                                                                                                                                  cGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTa   <  4:4027589/149‑1 (MQ=255)
                                                                                                                                                  cGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTa   <  4:1718555/149‑1 (MQ=255)
                                                                                                                                                   gTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTAc  <  1:2597428/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTAC  >  NC_000913/3447414‑3447709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: