Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,501,017 G→A 6.0% G370S (GGC→AGC)  frlA → putative fructoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,501,0170GA 6.0% 2463.4 / 88.8 752G370S (GGC→AGC) frlAputative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (353/350);  new base A (25/20);  total (379/373)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 764 total aligned reads displayed.

CGGCCATGTGCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTG  >  NC_000913/3500869‑3501165
                                                                                                                                                    |                                                                                                                                                    
 ggCCATGTGCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCgg                                                                                                                                                     <  4:3629671/149‑1 (MQ=255)
  gCCATGTGCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGc                                                                                                                                                    <  2:5693935/149‑1 (MQ=255)
    cATGTGCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGAGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTc                                                                                                                                                  <  4:1588638/149‑1 (MQ=255)
     aTGTGCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTcc                                                                                                                                                 <  1:7225031/149‑1 (MQ=255)
      tgtgCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCca                                                                                                                                                <  2:6368940/149‑1 (MQ=255)
        tgCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCcatc                                                                                                                                              >  2:1327488/1‑149 (MQ=255)
           aTCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCatcatc                                                                                                                                           <  4:4604617/149‑1 (MQ=255)
           aTCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCatcatc                                                                                                                                           >  1:2247651/1‑149 (MQ=255)
            tCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCt                                                                                                                                          >  1:1020647/1‑149 (MQ=255)
            tCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCt                                                                                                                                          <  4:3055478/149‑1 (MQ=255)
            tCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCt                                                                                                                                          >  3:1525529/1‑149 (MQ=255)
               gAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGgt                                                                                                                                       >  3:6988488/1‑149 (MQ=255)
                aaaTACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGgtg                                                                                                                                      <  3:1966159/149‑1 (MQ=255)
                 aaTACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGgtgt                                                                                                                                     <  4:4020304/149‑1 (MQ=255)
                   tACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCg                                                                                                                                   >  1:3625690/1‑149 (MQ=255)
                   tACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCg                                                                                                                                   >  3:6493983/1‑149 (MQ=255)
                    acaacaCGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGGTCCATCATCTGGTGTCGt                                                                                                                                  <  1:2118579/149‑1 (MQ=255)
                      aacaCGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTaa                                                                                                                                >  4:820804/1‑149 (MQ=255)
                      aacaCGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTaa                                                                                                                                >  2:1725550/1‑149 (MQ=255)
                        cacGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTTTTCATCTGCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGGCCTAAAc                                                                                                                              >  4:1380663/1‑149 (MQ=255)
                         acGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAAcg                                                                                                                             >  2:5983434/1‑149 (MQ=255)
                            ccGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGcga                                                                                                                          <  3:2654097/149‑1 (MQ=255)
                              ggATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGcgacg                                                                                                                        >  4:6362769/1‑149 (MQ=255)
                               gATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGcgacga                                                                                                                       >  1:4199984/1‑149 (MQ=255)
                               gATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGcgacga                                                                                                                       <  1:5748330/149‑1 (MQ=255)
                                    ctcCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACa                                                                                                                  >  3:5734328/1‑149 (MQ=255)
                                     tcCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTg                                                                                                                                         >  1:5426337/1‑125 (MQ=255)
                                     tcCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACaa                                                                                                                 >  3:5097058/1‑149 (MQ=255)
                                       catcatCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAAc                                                                                                               >  2:2004646/1‑149 (MQ=255)
                                       catcatCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAAc                                                                                                               >  2:2258959/1‑149 (MQ=255)
                                        atcataCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAAcc                                                                                                              >  4:3848437/1‑149 (MQ=255)
                                          catcCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGc                                                                                                            >  1:4510097/1‑149 (MQ=255)
                                            tcCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCtg                                                                                                          >  2:5368069/1‑149 (MQ=255)
                                                 cAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCg                                                                                                     <  1:3219750/149‑1 (MQ=255)
                                                  aaGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGt                                                                                                    >  4:5011986/1‑149 (MQ=255)
                                                       gcgcTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCcatc                                                                                                                                              >  3:992079/1‑102 (MQ=255)
                                                        cgcTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCg                                                                                              <  2:4358352/149‑1 (MQ=255)
                                                         gcTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAAcgc                                                                                                                            <  1:2761148/118‑1 (MQ=255)
                                                           tGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCt                                                                                           >  3:1733521/1‑149 (MQ=255)
                                                             gggATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCtgtg                                                                                                        >  2:1230847/1‑134 (MQ=255)
                                                             gggATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCttt                                                                                         <  2:6133874/149‑1 (MQ=255)
                                                              ggATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCGGTGGCGTACTCCGGCTTTc                                                                                        <  3:4185103/149‑1 (MQ=255)
                                                                aTCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCgg                                                                                      >  4:5682506/1‑149 (MQ=255)
                                                                   ttcttcATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCGTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAGACCGCTGTGGCGTACTCCGGCTTTCGGGCt                                                                                   >  1:4708365/1‑149 (MQ=255)
                                                                    tcttcATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCgg                                                                                      >  3:357184/1‑145 (MQ=255)
                                                                     cttcATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCtga                                                                                 <  4:682840/149‑1 (MQ=255)
                                                                      ttcATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCtgat                                                                                <  4:2657725/149‑1 (MQ=255)
                                                                        cATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCtgatga                                                                              <  4:693779/149‑1 (MQ=255)
                                                                        cATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCtgatga                                                                              <  4:1740791/149‑1 (MQ=255)
                                                                          tCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTGTCAAAAATTCCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTCGCGTACTCCGGCTTTCGGGCTGATGacc                                                                            <  4:4373479/149‑1 (MQ=255)
                                                                            ttCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTa                                                                                                                    >  2:2808410/1‑107 (MQ=255)
                                                                            ttCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGaccac                                                                          >  3:6865329/1‑149 (MQ=255)
                                                                            ttCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGaccac                                                                          >  1:5024760/1‑149 (MQ=255)
                                                                                tttCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGcgacga                                                                                                                       <  2:6264409/100‑1 (MQ=255)
                                                                                tttCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCgg                                                                                             <  1:6878921/126‑1 (MQ=255)
                                                                                tttCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTc                                                                      >  2:5544957/1‑149 (MQ=255)
                                                                                  tCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGc                                                                    <  2:1101760/149‑1 (MQ=255)
                                                                                  tCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGc                                                                    <  1:609476/149‑1 (MQ=255)
                                                                                       tctcACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTg                                                               <  4:4180649/149‑1 (MQ=255)
                                                                                       tctcACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTg                                                               >  2:2415348/1‑149 (MQ=255)
                                                                                        taacccAGCAGGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCCTAAACGCGACGATTACAAACCGCTGTGGCg                                                                                                     >  3:220164/5‑110 (MQ=255)
                                                                                         tcACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCg                                                             >  2:7240195/1‑149 (MQ=255)
                                                                                           aCCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTc                                                           >  3:2473914/1‑149 (MQ=255)
                                                                                           aCCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTc                                                           >  2:4918615/1‑149 (MQ=255)
                                                                                             cAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGc                                                              <  2:6819924/144‑1 (MQ=255)
                                                                                             cAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCaa                                                         <  3:4758680/149‑1 (MQ=255)
                                                                                               gCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGcgac                                                                                                                         >  3:1324304/1‑83 (MQ=255)
                                                                                                     tgGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCAtt                                                 >  4:2885420/1‑149 (MQ=255)
                                                                                                     tgGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCATACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCAtt                                                 <  4:6034643/149‑1 (MQ=255)
                                                                                                        gTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTTTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTg                                              <  2:3332844/149‑1 (MQ=255)
                                                                                                        gTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGAtt                                                                                                                     >  1:6359196/1‑78 (MQ=255)
                                                                                                         ttATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTgg                                             >  3:3796682/1‑149 (MQ=255)
                                                                                                          tATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGt                                            <  1:6616613/149‑1 (MQ=255)
                                                                                                           aTTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTc                                           >  4:3376173/1‑149 (MQ=255)
                                                                                                               cACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTAGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCGGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt                                       >  4:215812/1‑149 (MQ=255)
                                                                                                                 cccTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCtgatg                                                                               <  1:1512434/107‑1 (MQ=255)
                                                                                                                 cccTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCa                                     >  3:6190506/1‑149 (MQ=255)
                                                                                                                    tGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTGCATCATCGGGTGTCGTAAACGAGACGGTTACAAACCGCTGTGGCGTACTCGGGCTTTCGGGCTGATGACCAACCTCGCCGTTGCGTCAAGCCTCATTCTGGTCGCCTCAAcc                                  >  3:5994882/1‑149 (MQ=255)
                                                                                                                    tGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAAcc                                  <  1:1205935/149‑1 (MQ=255)
                                                                                                                     ggTGATGTGCTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGACTCATTCTGGTCGCCTCAACCt                                 >  1:6243902/1‑149 (MQ=255)
                                                                                                                      gTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCtt                                >  4:2472843/1‑149 (MQ=255)
                                                                                                                      gTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCtt                                >  3:3088613/1‑149 (MQ=255)
                                                                                                                       tGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCTCCTCAACCttt                               <  3:5600499/149‑1 (MQ=255)
                                                                                                                         aTGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTgg                             >  1:1817301/1‑148 (MQ=255)
                                                                                                                         aTGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGt                             >  1:388092/1‑149 (MQ=255)
                                                                                                                          tgtgTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTc                            <  4:5321599/149‑1 (MQ=255)
                                                                                                                            tgtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTg                          <  3:4532225/149‑1 (MQ=255)
                                                                                                                             gtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCATCCTTTGTCTgg                         <  3:4042820/149‑1 (MQ=255)
                                                                                                                             gtTTCAAAAATACCCTCACCTTCAGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCg                                                                     >  1:2491715/1‑105 (MQ=255)
                                                                                                                              tttCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTggg                        <  3:3376173/149‑1 (MQ=255)
                                                                                                                                   aaaaTACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTTCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCg                   >  4:62262/1‑149 (MQ=255)
                                                                                                                                         cccTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCAtt                                                 <  2:2698094/113‑1 (MQ=255)
                                                                                                                                          ccTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCg            <  3:753939/149‑1 (MQ=255)
                                                                                                                                           cTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTg                          <  1:939779/134‑1 (MQ=255)
                                                                                                                                           cTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTg                          >  2:939779/1‑134 (MQ=255)
                                                                                                                                              aCCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGt                             >  2:4702118/1‑128 (MQ=255)
                                                                                                                                              aCCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCt        >  1:6013074/1‑149 (MQ=255)
                                                                                                                                                cTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCtga                                                                                 <  1:1274981/74‑1 (MQ=255)
                                                                                                                                                cTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTa      >  3:5366643/1‑149 (MQ=255)
                                                                                                                                                  tCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATc    <  2:3876226/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGCCATGTGCATCCGAAATACAACACGCCGGATGTCTCCATCATCCTGCAAGGGGCGCTGGGGATCTTCTTCATCTTCGTTTCCGATCTCACCAGCCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTG  >  NC_000913/3500869‑3501165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: