| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,130,614 | G→T | 8.4% | L260L (CTG→CTT) | metL → | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,130,614 | 0 | G | T | 8.4% | 1984.2 / 121.6 | 576 | L260L (CTG→CTT) | metL | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base G (275/252); new base T (23/26); total (298/278) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-03 | |||||||||||
Only 100 of 585 total aligned reads displayed.
ACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTA > NC_000913/4130466‑4130762 | tATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgc > 1:5596299/1‑149 (MQ=255) tATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgc < 2:5677053/149‑1 (MQ=255) aTTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgcg < 2:7248785/149‑1 (MQ=255) aTTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgcg > 1:4272923/1‑149 (MQ=255) ttCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgcgc > 1:6482676/1‑149 (MQ=255) ccGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgcgcgc < 4:56979/149‑1 (MQ=255) ccGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgcgcgc < 3:3788106/149‑1 (MQ=255) cgcgACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGcc > 3:6477611/1‑149 (MQ=255) ggTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGt > 2:1329510/1‑149 (MQ=255) gTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGtt > 1:5322341/1‑149 (MQ=255) gcTGGCGGGTGTTTCTCGCGTAACCATCTGAAGCGACGTCGCCGGAGTATACAGTGCCGACCCGCGTAAAGTGATAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGGCTGGCGGCTCCCGttcttc < 2:2839012/149‑1 (MQ=255) ggggGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGccc > 2:6602352/1‑149 (MQ=255) gtgtTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGt > 3:3305915/1‑149 (MQ=255) ttCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACtt < 4:4252335/149‑1 (MQ=255) ttCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACtt > 2:5914122/1‑149 (MQ=255) ttCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACtt > 1:2261414/1‑149 (MQ=255) ctcGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTa > 4:1341130/1‑149 (MQ=255) ctcGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTa < 3:5387335/149‑1 (MQ=255) ctcGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTa > 2:612628/1‑149 (MQ=255) tcGCGTAACCATCTGGAGCGACATCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTAc < 3:4380682/149‑1 (MQ=255) tcGCGTAACCATCTGCAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGCCTCCCGTTCTTCACGCCCGTACTTTAc > 2:3719126/1‑149 (MQ=255) cgcgTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACa < 3:238203/149‑1 (MQ=255) aaCCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCgg < 2:6992409/149‑1 (MQ=255) aaCCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCgg > 1:5943382/1‑149 (MQ=255) ccATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCgg < 2:1191937/147‑1 (MQ=255) cATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGttt > 4:4470201/1‑149 (MQ=255) cATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGttt > 3:1069520/1‑149 (MQ=255) aTCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGATCCCGTTCTCCACGCCCGTACTTTACAGCCGGTTTc < 4:6738351/149‑1 (MQ=255) tCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCt < 1:1512863/149‑1 (MQ=255) tCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCt < 3:471924/149‑1 (MQ=255) tGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTgg < 1:2959904/149‑1 (MQ=255) tGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTgg < 4:3303976/149‑1 (MQ=255) ggAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGc > 1:4001229/1‑149 (MQ=255) cGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGaaa < 2:5078347/149‑1 (MQ=255) gTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAAt > 1:1040738/1‑149 (MQ=255) gTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAAt > 3:938666/1‑149 (MQ=255) tCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATc < 1:3743276/149‑1 (MQ=255) gCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGTCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGa > 2:4841518/1‑149 (MQ=255) ccGGGGTATACAGTGCCGACCCGCGTAAAGTGAAATATGCCTGTCTGCCGGTGTTGGTACGTCTGGATGAGGCGAGCGAACTGGCGGTCCTGGGGGCACCCGTGCTACACGCTCGTACTTTATAGCCGTTTTCTGGTAGCGAAATCGAc < 4:3914544/149‑1 (MQ=255) ccGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGAc > 1:4939774/1‑149 (MQ=255) ggggTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCt < 1:6332128/149‑1 (MQ=255) gggTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTg < 1:2640340/149‑1 (MQ=255) gTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCa > 3:4131856/1‑149 (MQ=255) aCAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTg > 1:6891036/1‑149 (MQ=255) gTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACa < 3:2150534/112‑1 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCaa > 3:6637029/1‑140 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGt > 3:4513015/1‑149 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGt > 3:6805956/1‑149 (MQ=255) cGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTa < 4:549995/149‑1 (MQ=255) gACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAg < 3:1736948/149‑1 (MQ=255) cccGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGcc < 3:2677859/108‑1 (MQ=255) ccGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTa < 4:6439921/149‑1 (MQ=255) ccGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTa > 1:1833520/1‑149 (MQ=255) ccGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTa > 3:1355546/1‑149 (MQ=255) cgTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGAc > 1:2564637/1‑126 (MQ=255) cgTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTacac < 2:6482676/149‑1 (MQ=255) aaaGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGa > 1:7138084/1‑122 (MQ=255) gTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGa > 4:2671624/1‑149 (MQ=255) gTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGa > 2:3169911/1‑149 (MQ=255) gTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGa > 4:5991347/1‑149 (MQ=255) tGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGAt < 2:3860838/149‑1 (MQ=255) aaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCaa < 2:2620663/149‑1 (MQ=255) aaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCaa < 4:1786703/149‑1 (MQ=255) gATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTg > 1:1341440/1‑128 (MQ=255) gATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAgg < 2:4755350/149‑1 (MQ=255) gATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAgg > 1:778885/1‑149 (MQ=255) gcctgcctGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAg < 2:3289966/87‑1 (MQ=255) gcctgcctGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAg > 1:3289966/1‑87 (MQ=255) ctgcctgcTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCa > 3:5760367/1‑149 (MQ=255) ctgctgCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCAcgcg < 2:4299465/149‑1 (MQ=255) ctgctgCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCAcgcg > 2:631741/1‑149 (MQ=255) tgctgcCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACgcgc < 1:2816074/149‑1 (MQ=255) tgcCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCAtt < 4:51102/149‑1 (MQ=255) tgcCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCAtt > 4:3731520/1‑149 (MQ=255) ccGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGa > 1:6541893/1‑149 (MQ=255) ccGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGa > 1:3507202/1‑149 (MQ=255) gTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAc < 1:4635697/149‑1 (MQ=255) ttGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAcg > 1:4781913/1‑149 (MQ=255) tgctgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAcgc < 2:5548038/149‑1 (MQ=255) ctgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGt < 2:2179559/149‑1 (MQ=255) tgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACa < 4:1981570/67‑1 (MQ=255) tgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTg > 4:712817/1‑149 (MQ=255) tgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTg > 1:2051435/1‑149 (MQ=255) gTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTgcgc < 4:6237426/101‑1 (MQ=255) tCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTgg > 2:828955/1‑149 (MQ=255) tCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGAt < 3:6397457/118‑1 (MQ=255) cTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGc > 2:3448306/1‑149 (MQ=255) tGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGaa > 2:2487325/1‑137 (MQ=255) tGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGcc > 1:1706404/1‑149 (MQ=255) ggATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGa < 4:2299246/84‑1 (MQ=255) gATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCTGTACTTTACAGCCGGGTTCTGGCAGCGAAATCGACCTTCAACTTCGCTGCAGGTAAGCGCCTGCTCAAGGTGTCAGGCGCATTGAACGCTTGCTGGCCTc > 4:1145033/1‑149 (MQ=255) gAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCgg < 1:6124048/149‑1 (MQ=255) gAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCgg > 2:354418/1‑149 (MQ=255) ggCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTa > 2:158078/1‑149 (MQ=255) gCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTAc < 1:2444704/149‑1 (MQ=255) cAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTg < 2:1993350/149‑1 (MQ=255) aGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTgg > 3:6075386/1‑149 (MQ=255) gCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGt > 2:4555064/1‑149 (MQ=255) gAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTgc > 4:4746288/1‑149 (MQ=255) aaCTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTgcg > 3:1890386/1‑149 (MQ=255) | ACTATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTA > NC_000913/4130466‑4130762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |