Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,130,616 | C→A | 8.4% | A261E (GCG→GAG) | metL → | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,130,616 | 0 | C | A | 8.4% | 1891.7 / 116.6 | 554 | A261E (GCG→GAG) | metL | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
Reads supporting (aligned to +/- strand): ref base C (265/242); new base A (22/25); total (287/267) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.42e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 |
Only 100 of 589 total aligned reads displayed.
TATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATT > NC_000913/4130468‑4130764 | tATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgc > 1:5596299/1‑149 (MQ=255) tATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgc > 3:3729428/1‑149 (MQ=255) ttCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGgcgc > 1:6482676/1‑149 (MQ=255) cgcgACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGcc > 3:6477611/1‑149 (MQ=255) cgcgACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGcc < 4:1966140/149‑1 (MQ=255) gACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTgg > 4:5025587/1‑149 (MQ=255) aaaTCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCt > 3:1150982/1‑149 (MQ=255) aaaTCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCt > 3:2622996/1‑149 (MQ=255) aTCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTcc > 4:4051904/1‑149 (MQ=255) gTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCg > 2:6511154/1‑147 (MQ=255) gcTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGttcttc > 2:323568/1‑149 (MQ=255) tgtTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTa < 2:5372392/149‑1 (MQ=255) ttCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACtt > 1:2261414/1‑149 (MQ=255) ttCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCGCCCGTTCTTCACGCCCGTACtt < 4:6438153/149‑1 (MQ=255) ctcGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTa > 4:1341130/1‑149 (MQ=255) ctcGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTa < 3:5387335/149‑1 (MQ=255) tcGCGTAACCATCTGGAGCGACATCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTAc < 3:4380682/149‑1 (MQ=255) gTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGttc < 4:5693658/127‑1 (MQ=255) gTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGcc < 1:2445267/149‑1 (MQ=255) gTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGcc > 2:3844950/1‑149 (MQ=255) tAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGTTCCCGTTCTTCACGCCCGTACTTTACAGCCg < 1:2594629/149‑1 (MQ=255) aaCCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCgg < 1:5282339/149‑1 (MQ=255) aaCCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCgg > 1:5943382/1‑149 (MQ=255) aaCCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCgg > 2:736713/1‑149 (MQ=255) ccATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGtt < 2:5621218/149‑1 (MQ=255) aTCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTc > 4:4607549/1‑149 (MQ=255) tCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCt < 3:471924/149‑1 (MQ=255) tCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCt < 3:4049265/149‑1 (MQ=255) tCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCt < 1:1512863/149‑1 (MQ=255) tCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCt < 2:1284021/149‑1 (MQ=255) ggAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGc > 4:5855614/1‑149 (MQ=255) ggAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGc > 2:2711690/1‑149 (MQ=255) gTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAAt > 1:1040738/1‑149 (MQ=255) gTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAAt > 3:938666/1‑149 (MQ=255) tCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATc < 3:1665632/149‑1 (MQ=255) tCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATc < 4:6198997/149‑1 (MQ=255) ggggTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCt < 1:6332128/149‑1 (MQ=255) ggTATACAGTGCCGACCCGCGTAAAGCAACAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGACGCGCCTGGCGTCTCCCGCTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGc < 2:4972521/149‑1 (MQ=255) gTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCa > 2:944975/1‑149 (MQ=255) gTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCa > 2:4461330/1‑149 (MQ=255) aCAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTg > 1:6891036/1‑149 (MQ=255) cAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTgc > 1:2675622/1‑149 (MQ=255) tGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCt < 4:170823/149‑1 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCaa > 3:6637029/1‑140 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGt > 3:4513015/1‑149 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGt < 4:3729428/149‑1 (MQ=255) ccGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGt > 3:6805956/1‑149 (MQ=255) ccGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTa > 2:619400/1‑149 (MQ=255) ccGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTa > 1:1833520/1‑149 (MQ=255) gTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACg > 4:2984335/1‑149 (MQ=255) aaaGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGcc > 2:6837232/1‑149 (MQ=255) tGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGAt < 2:2605994/149‑1 (MQ=255) gAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATc > 1:760936/1‑149 (MQ=255) aaaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCa > 2:1761750/1‑149 (MQ=255) aaaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCa > 4:5379569/1‑149 (MQ=255) aaaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCa > 2:2960522/1‑149 (MQ=255) acgATGCCTGACTGCTGCCGTTGCTGCGTCTGGATGATCCCAGCTGCCTTGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCaa < 2:3509719/147‑1 (MQ=255) aaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCaa < 1:2820679/149‑1 (MQ=255) aaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCaa < 2:1040738/149‑1 (MQ=255) aaGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCaa > 2:2481070/1‑149 (MQ=255) aGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAg < 4:3853568/149‑1 (MQ=255) gATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTg > 1:1341440/1‑128 (MQ=255) tgcctgccTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGtt < 4:2597141/149‑1 (MQ=255) tgcctgccTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGtt < 1:400528/149‑1 (MQ=255) gcctgcctGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAg > 1:3289966/1‑87 (MQ=255) gcctgcctGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTc < 1:2960522/149‑1 (MQ=255) ctgcctgcTGCCGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCa > 2:212876/1‑149 (MQ=255) ctgcctgcTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCa > 2:3645952/1‑149 (MQ=255) ctgcctgcTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGCCGCGCCTGGCGGCTGCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACTCGCCGGATCAAGGTGCCa > 2:7191159/1‑149 (MQ=255) tgcctgcTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCAc < 2:411743/149‑1 (MQ=255) gctgcCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCa > 3:2058955/1‑149 (MQ=255) gctgcCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCa > 2:1500852/1‑149 (MQ=255) ctgcCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCg > 4:6691435/1‑55 (MQ=255) ccGTTGCTGCGTCTGGATGAGGCCAGCGACCTTGAGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGa > 1:6541893/1‑149 (MQ=255) ccGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGa > 1:3507202/1‑149 (MQ=255) ccGTTGCTGCGTCTCGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGa < 2:6362822/149‑1 (MQ=255) cGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCAGGCCCGTACTTTACAGCCGGTTTCTGGCACCGAAGTCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGAGCATTGaa > 4:4396007/1‑149 (MQ=255) gTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAc < 1:4635697/149‑1 (MQ=255) ttGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAcg > 2:3343495/1‑149 (MQ=255) ttGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAcg > 1:4781913/1‑149 (MQ=255) ctgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGt < 2:4781913/149‑1 (MQ=255) tgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTg > 1:2051435/1‑149 (MQ=255) tgcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTg > 4:712817/1‑149 (MQ=255) gcGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGc > 1:427650/1‑149 (MQ=255) gTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGTTg < 2:574015/149‑1 (MQ=255) tCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGAt < 3:6397457/118‑1 (MQ=255) tGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGcc > 1:1706404/1‑149 (MQ=255) ggATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGa > 3:2299246/1‑84 (MQ=255) gATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCTGTACTTTACAGCCGGGTTCTGGCAGCGAAATCGACCTTCAACTTCGCTGCAGGTAAGCGCCTGCTCAAGGTGTCAGGCGCATTGAACGCTTGCTGGCCTc > 4:1145033/1‑149 (MQ=255) gATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTc > 1:7108397/1‑149 (MQ=255) gAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAAc < 1:4933753/133‑1 (MQ=255) gAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCgg < 1:6124048/149‑1 (MQ=255) gCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTcc > 4:2269188/1‑117 (MQ=255) gCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTAc > 3:1871110/1‑149 (MQ=255) gCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTAc > 3:3206083/1‑149 (MQ=255) ccAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACt < 1:3778886/149‑1 (MQ=255) aGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTgg > 2:5993879/1‑149 (MQ=255) aGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTgg > 3:6075386/1‑149 (MQ=255) aCTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTgcgc < 3:5749074/149‑1 (MQ=255) tGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGcnngn < 2:4563213/149‑2 (MQ=255) | TATTCCGCGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCGCGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGTTCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATT > NC_000913/4130468‑4130764 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |