BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,285,725	A→G	7.4%	G549G (GGT→GGC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,725	0	A	G	7.4%	2121.9 / 106.3	675	G549G (GGT→GGC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (333/291); new base G (24/26); total (357/318)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 686 total aligned reads displayed.

TCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGT  >  NC_000913/4285577‑4285873

tCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATa                                                                                                                                                      >  1:6112030/1‑149 (MQ=255)

 ccAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATAc                                                                                                                                                     >  3:3044852/1‑149 (MQ=255)

    gTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACcta                                                                                                                                                  >  1:7016539/1‑149 (MQ=255)

     tGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACctac                                                                                                                                                 <  2:7016539/149‑1 (MQ=255)

          caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcgg                                                                                                                                            >  1:1028320/1‑149 (MQ=255)

           aCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcggc                                                                                                                                           >  3:4082836/1‑149 (MQ=255)

             gTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcggcgg                                                                                                                                         >  2:310813/1‑149 (MQ=255)

                 ggCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTc                                                                                                                                     <  3:806489/149‑1 (MQ=255)

                 ggCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTc                                                                                                                                     <  4:3150576/149‑1 (MQ=255)

                  gCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCg                                                                                                                                    <  3:2600964/149‑1 (MQ=255)

                      cGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCaa                                                                                                                                <  1:5610149/149‑1 (MQ=255)

                       gCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAAt                                                                                                                               >  1:6659160/1‑149 (MQ=255)

                         cAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCt                                                                                                                             >  3:2425673/1‑149 (MQ=255)

                          aGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCtt                                                                                                                            >  4:2152606/1‑149 (MQ=255)

                            cGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAAGATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                                                                                          <  1:536932/149‑1 (MQ=255)

                               gCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                                                                                       >  1:2359241/1‑149 (MQ=255)

                               gCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                                                                                       >  4:4683225/1‑149 (MQ=255)

                                 cAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                                                                                     >  3:5673601/1‑149 (MQ=255)

                                 cAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                                                                                     <  2:1477991/149‑1 (MQ=255)

                                 cAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                                                                                     >  1:1339179/1‑149 (MQ=255)

                                 cAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                                                                                     >  1:486345/1‑149 (MQ=255)

                                 cAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgc                                                                                                                     >  1:2561053/1‑149 (MQ=255)

                                        tttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccacca                                                                                                              >  1:4348684/1‑149 (MQ=255)

                                               aCCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATGCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGa                                                                                                       <  1:4595787/149‑1 (MQ=255)

                                                cccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGAc                                                                                                      <  2:2703884/149‑1 (MQ=255)

                                                     ttGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAt                                                                                                 <  3:3619692/149‑1 (MQ=255)

                                                        cGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAtctc                                                                                              >  4:2957385/1‑149 (MQ=255)

                                                         ggACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATctct                                                                                             >  1:150493/1‑149 (MQ=255)

                                                         ggACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATctct                                                                                             <  3:2285335/149‑1 (MQ=255)

                                                         ggACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATctct                                                                                             <  2:6167541/149‑1 (MQ=255)

                                                          gACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCAACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTg                                                                                            <  2:6882189/149‑1 (MQ=255)

                                                           aCTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTTCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGc                                                                                           <  4:4946722/149‑1 (MQ=255)

                                                             ttCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                                                                                        >  4:5420539/1‑118 (MQ=255)

                                                             ttCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCg                                                                                         >  4:1596578/1‑149 (MQ=255)

                                                                tGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcc                                                                                      >  2:4655638/1‑149 (MQ=255)

                                                                 gCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTccc                                                                                     >  4:395303/1‑149 (MQ=255)

                                                                   gTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATGCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCa                                                                                   <  3:3759206/149‑1 (MQ=255)

                                                                    tACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAg                                                                                  >  4:2631895/1‑149 (MQ=255)

                                                                           cTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGa                                                                           <  3:325119/149‑1 (MQ=255)

                                                                            tGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGAc                                                                          >  4:5828419/1‑149 (MQ=255)

                                                                              gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCg                                                                        <  1:3443256/149‑1 (MQ=255)

                                                                               tGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCgg                                                                       >  3:6764408/1‑149 (MQ=255)

                                                                                gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGga                                                                      <  3:5831616/149‑1 (MQ=255)

                                                                                 aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGAGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCACCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgag                                                                     >  2:3949146/1‑149 (MQ=255)

                                                                                  cGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga                                                                    <  3:7055616/149‑1 (MQ=255)

                                                                                  cGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga                                                                    <  3:1667037/149‑1 (MQ=255)

                                                                                   ggTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGAc                                                                   <  3:3929023/149‑1 (MQ=255)

                                                                                    gTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACg                                                                  >  3:1400630/1‑149 (MQ=255)

                                                                                       cctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTc                                                                                                                           >  1:3625046/1‑89 (MQ=255)

                                                                                       cctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTc                                                               <  4:788306/149‑1 (MQ=255)

                                                                                       cctccACGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTc                                                                                                                           <  2:3625046/89‑1 (MQ=255)

                                                                                         tccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                                                                                         <  2:168393/105‑1 (MQ=255)

                                                                                         tccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                                                                                         >  1:168393/1‑105 (MQ=255)

                                                                                         tccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAg                                                             >  3:6542216/1‑149 (MQ=255)

                                                                                          ccccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCa                                                              <  2:2289316/147‑1 (MQ=255)

                                                                                           cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATGcc                                                                                                                                                    >  1:2959545/1‑60 (MQ=255)

                                                                                           cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCa                                                              >  4:2450877/1‑146 (MQ=255)

                                                                                            ccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCAc                                                          >  1:2120862/1‑149 (MQ=255)

                                                                                            ccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCAc                                                          <  2:1309528/149‑1 (MQ=255)

                                                                                             cGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCAcg                                                         >  2:6054150/1‑149 (MQ=255)

                                                                                             cGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCAcg                                                         >  4:6018063/1‑149 (MQ=255)

                                                                                             cGTGATT‑AGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCAcgt                                                        >  1:5868359/1‑149 (MQ=255)

                                                                                                gATTAAGCGTGACTTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCGGACGGTGACCGGAGACGGTCAGCAcgtcg                                                      >  4:106812/1‑149 (MQ=255)

                                                                                                 aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATGCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCAcgtcgt                                                     <  2:5242126/149‑1 (MQ=255)

                                                                                                  ttAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATGCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACgtcgtc                                                    >  2:4262315/1‑149 (MQ=255)

                                                                                                   tAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTcc                                                   >  2:1949806/1‑149 (MQ=255)

                                                                                                   tAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTcc                                                   <  3:5081289/149‑1 (MQ=255)

                                                                                                      gCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCcaca                                                >  3:4477345/1‑149 (MQ=255)

                                                                                                           aCGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACgtcgtc                                                    <  3:5274688/140‑1 (MQ=255)

                                                                                                           aCGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGccc                                           >  1:890289/1‑149 (MQ=255)

                                                                                                             gTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGTTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTccc                                                                                     >  4:6719808/1‑105 (MQ=255)

                                                                                                             gTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCgg                                         >  1:3215504/1‑149 (MQ=255)

                                                                                                                 gCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTAt                                     >  2:6408020/1‑149 (MQ=255)

                                                                                                                 gCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTAt                                     >  3:4868536/1‑149 (MQ=255)

                                                                                                                  cGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCTGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                    <  2:5602307/149‑1 (MQ=255)

                                                                                                                  cGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                    <  4:1716925/149‑1 (MQ=255)

                                                                                                                   gTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATcc                                   >  2:1156302/1‑149 (MQ=255)

                                                                                                                   gTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATcc                                   >  1:4157732/1‑149 (MQ=255)

                                                                                                                       aTCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCa                                  >  2:3475800/1‑146 (MQ=255)

                                                                                                                       aTCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAg                                 <  2:50265/147‑1 (MQ=255)

                                                                                                                       aTCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAg                                 >  1:50265/1‑147 (MQ=255)

                                                                                                                         cGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGACCACACGCCCGGTTATCCAGTAAt                             <  3:2772743/149‑1 (MQ=255)

                                                                                                                          gCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATa                            <  4:4300722/149‑1 (MQ=255)

                                                                                                                             tGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGcc                         <  4:1177822/149‑1 (MQ=255)

                                                                                                                              gACCTTTAGTATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGc                          <  1:1820827/147‑1 (MQ=255)

                                                                                                                               aCCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGTAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCAt                       >  4:647980/1‑149 (MQ=255)

                                                                                                                               aCCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGt                                >  4:5256868/1‑140 (MQ=255)

                                                                                                                                ccTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATc                      >  3:1748605/1‑149 (MQ=255)

                                                                                                                                  tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATctct                                                                                             >  2:6641444/1‑76 (MQ=255)

                                                                                                                                  tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCtt                    >  4:6940977/1‑149 (MQ=255)

                                                                                                                                  tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCtt                    <  4:3863773/149‑1 (MQ=255)

                                                                                                                                   ttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                    >  3:1164213/1‑132 (MQ=255)

                                                                                                                                    tAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCa                  <  2:245946/149‑1 (MQ=255)

                                                                                                                                          tgtgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGc                          >  1:3875014/1‑135 (MQ=255)

                                                                                                                                          tgtgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGa            <  1:1240097/149‑1 (MQ=255)

                                                                                                                                            tgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAcg          >  1:6443001/1‑149 (MQ=255)

                                                                                                                                             gCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAcgc         <  3:6018063/149‑1 (MQ=255)

                                                                                                                                               ggAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAcggcg       >  2:762030/1‑146 (MQ=255)

                                                                                                                                                 aaTACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACgcgcgc     >  3:1955543/1‑149 (MQ=255)

                                                                                                                                                  aTACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGcc    >  2:3846418/1‑149 (MQ=255)

TCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGT  >  NC_000913/4285577‑4285873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`