Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,450,950 C→T 5.7% intergenic (+32/‑108) ytfQ → / → ytfR galactofuranose ABC transporter periplasmic binding protein/putative sugar ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,450,9500CT 5.7% 2835.9 / 100.2 856intergenic (+32/‑108)ytfQ/ytfRgalactofuranose ABC transporter periplasmic binding protein/putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (391/413);  new base T (25/24);  total (418/438)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

Only 100 of 876 total aligned reads displayed.

GAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGG  >  NC_000913/4450802‑4451098
                                                                                                                                                    |                                                                                                                                                    
gagaAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGcc                                                                                                                                                      >  1:2910561/1‑149 (MQ=255)
 agaAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCg                                                                                                                                                     <  4:6801390/149‑1 (MQ=255)
  gaAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCgg                                                                                                                                                    >  1:4378888/1‑149 (MQ=255)
     aTACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATa                                                                                                                                                 >  4:7120707/1‑149 (MQ=255)
      tACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATaa                                                                                                                                                >  2:839754/1‑149 (MQ=255)
      tACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATaa                                                                                                                                                >  4:2781138/1‑149 (MQ=255)
      tACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATaa                                                                                                                                                >  2:5007120/1‑149 (MQ=255)
       gcAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCCCCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATTGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg                                                                                                                                               <  3:1466095/148‑1 (MQ=255)
       aCAAAAAAGACGGCACCATGCCTGAAAAGCAGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg                                                                                                                                               <  3:5145001/149‑1 (MQ=255)
       aCAAAAAAGACGGCACCATGCATGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAAATAGAGAAGAAGAAAAATATGGGGTATTGAGGTTTGATATGCCTGATGCCTTTTCATAGGCAGGATAAg                                                                                                                                               <  4:1416994/149‑1 (MQ=255)
         aaaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                             >  4:1636568/1‑149 (MQ=255)
         aaaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                             >  1:6911536/1‑149 (MQ=255)
          aaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgcg                                                                                                                                            >  2:3764458/1‑149 (MQ=255)
          aaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgcg                                                                                                                                            >  2:6466916/1‑149 (MQ=255)
           aaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgcgc                                                                                                                                           <  3:4639804/149‑1 (MQ=255)
                       cATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                             <  3:4890343/135‑1 (MQ=255)
                       cATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATc                                                                                                                               >  3:1178497/1‑149 (MQ=255)
                             tGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa                                                                                                                         <  1:839754/149‑1 (MQ=255)
                              gAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGAt                                                                                                                        <  1:5838824/149‑1 (MQ=255)
                                     tGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCAc                                                                                                                 >  2:4164083/1‑149 (MQ=255)
                                     tGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCAc                                                                                                                 >  2:1058585/1‑149 (MQ=255)
                                           tAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGc                                                                                                           <  4:4690427/149‑1 (MQ=255)
                                           tAACCAAATCCACCCTTTACCCGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGc                                                                                                           <  1:2126610/149‑1 (MQ=255)
                                              ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGaa                                                                                                             <  4:4151337/144‑1 (MQ=255)
                                              ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                        >  1:5658948/1‑149 (MQ=255)
                                              ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                        >  2:6044432/1‑149 (MQ=255)
                                              ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                        >  2:5586506/1‑149 (MQ=255)
                                              ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                        >  3:1655888/1‑149 (MQ=255)
                                               cAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGAGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGa                                                                                                       >  1:3624749/1‑149 (MQ=255)
                                                aaaTCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                                                                      <  1:265521/149‑1 (MQ=255)
                                                      aCCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg                                                                                                                                               <  3:41230/102‑1 (MQ=255)
                                                      aCCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg                                                                                                >  4:3969847/1‑149 (MQ=255)
                                                      aCCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg                                                                                                >  2:2048653/1‑149 (MQ=255)
                                                      aCCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGACGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg                                                                                                >  1:4120256/1‑149 (MQ=255)
                                                        ccTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc                                                                                                                                    <  2:6670572/111‑1 (MQ=255)
                                                        ccTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt                                                                                              >  1:2360719/1‑149 (MQ=255)
                                                          tttACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATAGGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCCATGGTGCACTGAAGCCTGATGCGACGCTTa                                                                                            >  1:4708457/1‑149 (MQ=255)
                                                              cctgcctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                                                                        <  3:6519144/149‑1 (MQ=255)
                                                              cctgcctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                                                                        <  1:1058585/149‑1 (MQ=255)
                                                                tgcctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                                                                      >  2:1935434/1‑149 (MQ=255)
                                                                    tgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                                                                  <  2:3890632/149‑1 (MQ=255)
                                                                       tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                                                                               <  2:1636692/149‑1 (MQ=255)
                                                                        aCCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                                                                              <  3:3969847/149‑1 (MQ=255)
                                                                        aCCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                                                                              <  2:258090/149‑1 (MQ=255)
                                                                             aaaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCTGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa                                                                         >  3:3732418/1‑149 (MQ=255)
                                                                             aaaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg                                                                                                                                               >  4:5020586/1‑79 (MQ=255)
                                                                              aaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAc                                                                        >  4:3477109/1‑149 (MQ=255)
                                                                                 gaagaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                                                                     <  1:4996466/149‑1 (MQ=255)
                                                                                  aagaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                                                                    >  1:6647911/1‑149 (MQ=255)
                                                                                    gaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGaa                                                                                                             <  4:5638835/106‑1 (MQ=255)
                                                                                     aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGc                                                                 >  3:3513302/1‑149 (MQ=255)
                                                                                      aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCa                                                                >  2:4742725/1‑149 (MQ=255)
                                                                                       ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                                                                           >  1:5260691/1‑121 (MQ=255)
                                                                                           agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCtt                                                           <  2:3357807/149‑1 (MQ=255)
                                                                                           agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCtt                                                           >  3:5673894/1‑149 (MQ=255)
                                                                                            gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCttt                                                          <  1:1620418/149‑1 (MQ=255)
                                                                                             aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCTGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTa                                                         <  4:1715898/149‑1 (MQ=255)
                                                                                             aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                             <  1:1145879/65‑1 (MQ=255)
                                                                                             aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTa                                                         <  2:447331/149‑1 (MQ=255)
                                                                                               gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                                                                   <  2:1217534/137‑1 (MQ=255)
                                                                                                  gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg                                                    >  4:3116651/1‑149 (MQ=255)
                                                                                                  gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg                                                    >  2:2286166/1‑149 (MQ=255)
                                                                                                  gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg                                                    >  3:7108689/1‑149 (MQ=255)
                                                                                                   aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCgg                                                   <  2:5795579/149‑1 (MQ=255)
                                                                                                      aaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggggg                                                <  3:2625377/149‑1 (MQ=255)
                                                                                                       atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGa                                               >  4:1999094/1‑149 (MQ=255)
                                                                                                       atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGa                                               <  3:6811682/149‑1 (MQ=255)
                                                                                                          tGGGGTATTGAGGGTTGCTATGCCTGATGCCGCTTCGGAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGATTGACACCGGGGGaatt                                            <  2:2854711/149‑3 (MQ=255)
                                                                                                           ggggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAAcc                                           <  4:5340750/149‑1 (MQ=255)
                                                                                                            gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGc                                                                                                           <  1:7134726/84‑1 (MQ=255)
                                                                                                            gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCa                                          >  3:6216176/1‑149 (MQ=255)
                                                                                                             ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCAt                                         <  2:3037204/149‑1 (MQ=255)
                                                                                                               tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATga                                       <  3:1829404/149‑1 (MQ=255)
                                                                                                                    aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                                                                      <  4:6583603/97‑1 (MQ=255)
                                                                                                                    aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGAcgacc                                  >  2:6263172/1‑149 (MQ=255)
                                                                                                                     gggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTaca                                                       <  3:5464727/127‑1 (MQ=255)
                                                                                                                     gggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggg                                                  >  4:2564707/1‑132 (MQ=255)
                                                                                                                       gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc                                                                            <  1:3128204/104‑1 (MQ=255)
                                                                                                                            tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAaca                          >  3:223201/1‑149 (MQ=255)
                                                                                                                            tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAaca                          >  4:6388962/1‑149 (MQ=255)
                                                                                                                              tGCCTGATGCCGATTCGTAGGCTGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACAcc                        <  3:2989247/149‑1 (MQ=255)
                                                                                                                               gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCa                       <  4:6244655/149‑1 (MQ=255)
                                                                                                                               gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCa                       >  4:3697203/1‑149 (MQ=255)
                                                                                                                                ccTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAg                      <  1:3856634/149‑1 (MQ=255)
                                                                                                                                 cTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAgg                     >  1:1311278/1‑149 (MQ=255)
                                                                                                                                  tGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGga                    <  3:6744323/149‑1 (MQ=255)
                                                                                                                                    aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGgaga                  <  1:645911/149‑1 (MQ=255)
                                                                                                                                     tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGa                                                                                                              >  1:1943929/1‑56 (MQ=255)
                                                                                                                                       ccGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtcc               <  3:2020208/149‑1 (MQ=255)
                                                                                                                                        cGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                                                                    >  4:5854282/1‑95 (MQ=255)
                                                                                                                                        cGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                                                                    <  3:5854282/95‑1 (MQ=255)
                                                                                                                                         gATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtcctc             <  3:829535/149‑1 (MQ=255)
                                                                                                                                           ttCGTAGGCCAGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggg                                                  >  1:333798/1‑110 (MQ=255)
                                                                                                                                              gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtcctcc            <  2:7154606/145‑1 (MQ=255)
                                                                                                                                               tAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCAcc       >  2:6938509/1‑149 (MQ=255)
                                                                                                                                                 ggCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACGGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGa     >  4:2348063/1‑149 (MQ=255)
                                                                                                                                                 ggCCGGATAACGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTc                                                                                     <  1:6231160/69‑1 (MQ=255)
                                                                                                                                                  gCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGaa    >  4:1198837/1‑149 (MQ=255)
                                                                                                                                                  gCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGaa    <  3:2431056/149‑1 (MQ=255)
                                                                                                                                                    cGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggg                                                  >  2:3502545/1‑101 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGG  >  NC_000913/4450802‑4451098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: