Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,701,843 Δ1 bp 8.8% intergenic (+9/+21) yhjV → / ← dppF putative transporter/dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,701,8430G. 8.8% 507.1 / 41.0 148intergenic (+9/+21)yhjV/dppFputative transporter/dipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base G (64/71);  new base . (5/8);  total (69/79)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 150 total aligned reads displayed.

TATTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGC  >  NC_000913/3701696‑3701989
                                                                                                                                                   |                                                                                                                                                   
   tgtgTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATg                                                                                                                                                 <  2:1183904/149‑1 (MQ=255)
          tGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCgg                                                                                                                                         >  3:58595/1‑149 (MQ=255)
            ccTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtt                                                                                                                                       >  2:253175/1‑149 (MQ=255)
             cTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCgg                                                                                                                                         >  3:482380/1‑146 (MQ=255)
             cTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGttt                                                                                                                                      >  1:206409/1‑149 (MQ=255)
              tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                     >  1:1038940/1‑149 (MQ=255)
              tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                     <  2:206409/149‑1 (MQ=255)
                aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                   <  2:564057/149‑1 (MQ=255)
                 ttCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgtg                                                                                                                                  >  2:62693/1‑149 (MQ=255)
                  tCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                 <  4:251554/149‑1 (MQ=255)
                   ccGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAg                                                                                                                                >  4:506165/1‑149 (MQ=255)
                     gTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtt                                                                                                                                       >  2:711695/1‑140 (MQ=255)
                      tttttCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCAGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTa                                                                                                                             <  1:253175/149‑1 (MQ=255)
                               aTCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCgg                                                                                                                    >  4:708392/1‑149 (MQ=255)
                                tCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                   <  1:689632/133‑1 (MQ=255)
                                  tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtt                                                                                                                 >  1:1010423/1‑149 (MQ=255)
                                   ataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                <  1:62693/149‑1 (MQ=255)
                                   ataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                <  3:739968/149‑1 (MQ=255)
                                   ataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                <  4:129031/149‑1 (MQ=255)
                                    taAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                     <  3:174393/127‑1 (MQ=255)
                                    taAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                     >  4:174393/1‑127 (MQ=255)
                                       aGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGa                                                                                                                   >  1:565357/1‑142 (MQ=255)
                                       aGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGa                                                                                                                   <  2:565357/142‑1 (MQ=255)
                                        gTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                           <  3:708392/149‑1 (MQ=255)
                                         tCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                 >  1:740372/1‑126 (MQ=255)
                                         tCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                 <  2:740372/126‑1 (MQ=255)
                                          cGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCt                                                                                                         <  3:1082126/149‑1 (MQ=255)
                                           gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAt                                                                                                                  <  4:1131/139‑1 (MQ=255)
                                           gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAt                                                                                                                  >  3:1131/1‑139 (MQ=255)
                                             acaACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGAt                                                                                                      <  3:506165/149‑1 (MQ=255)
                                             acaACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGAt                                                                                                      >  4:1152077/1‑149 (MQ=255)
                                                  tGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACa                                                                                                 >  1:1308216/1‑149 (MQ=255)
                                                    gAAAAACTTCGGGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactca                                                                                                                                 >  2:864931/1‑110 (MQ=37)
                                                    gAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactca                                                                                                                                 <  1:864931/115‑6 (MQ=38)
                                                    gAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactca                                                                                                                                 >  2:628831/1‑110 (MQ=38)
                                                    gAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                   <  4:710045/113‑1 (MQ=255)
                                                     aaaaaCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCa                                                                                              >  4:1340888/1‑149 (MQ=255)
                                                      aaaaCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaa                                                                                             >  2:1188656/1‑149 (MQ=255)
                                                        aaCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaaaa                                                                                           <  4:642157/149‑1 (MQ=255)
                                                           ttCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAg                                                                                                <  1:369953/141‑1 (MQ=255)
                                                           ttCGCGGATTTAAAGCCTGACTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTCTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAg                                                                                                >  2:369953/1‑141 (MQ=255)
                                                                ggATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                           <  4:1329368/125‑1 (MQ=255)
                                                                ggATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATc                                                                                                           >  3:1329368/1‑125 (MQ=255)
                                                                    ttAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGt                                                                               <  3:1152077/149‑1 (MQ=255)
                                                                     tAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGtt                                                                              <  4:1466974/149‑1 (MQ=255)
                                                                      aaaGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGAt                                                                                                      <  1:1055659/124‑1 (MQ=255)
                                                                         gCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAcc                                                                          <  2:1308216/149‑1 (MQ=255)
                                                                          ccTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                                                     >  3:1435403/1‑137 (MQ=255)
                                                                          ccTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                                                     <  4:1435403/137‑1 (MQ=255)
                                                                             ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccgccg                                                                      <  1:540663/149‑1 (MQ=255)
                                                                              gCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtt                                                                                                                 <  4:1135868/105‑1 (MQ=255)
                                                                              gCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccgcctt                                                                     >  2:237239/1‑147 (MQ=255)
                                                                                     ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCttt                                                              >  3:644874/1‑149 (MQ=255)
                                                                                      tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                    >  2:910132/1‑78 (MQ=255)
                                                                                      tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                    <  1:910132/78‑1 (MQ=255)
                                                                                      tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAg                                                                                                >  1:256815/1‑114 (MQ=255)
                                                                                      tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAg                                                                                                <  2:256815/114‑1 (MQ=255)
                                                                                      tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                                             <  2:1038940/149‑1 (MQ=255)
                                                                                       gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAATTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                                            >  3:1263480/1‑149 (MQ=255)
                                                                                          gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaaaa                                                                                           >  1:856701/1‑115 (MQ=255)
                                                                                          gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCt                                                         <  1:237239/149‑1 (MQ=255)
                                                                                              ggCATTTTGCTATGCTTGTCGCTACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTTGTCTTTTAg                                                           <  4:938100/143‑1 (MQ=255)
                                                                                              ggCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtt                                                                                                                                       <  3:992199/67‑1 (MQ=25)
                                                                                              ggCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                                             <  1:720131/93‑1 (MQ=255)
                                                                                              ggCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                                             <  1:615467/93‑1 (MQ=255)
                                                                                              ggCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                                                      >  4:591510/1‑116 (MQ=255)
                                                                                              ggCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                                                      <  3:591510/116‑1 (MQ=255)
                                                                                              ggCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                                           >  3:938100/1‑143 (MQ=255)
                                                                                               gCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                                                 >  1:442178/1‑120 (MQ=255)
                                                                                               gCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                                                 <  2:442178/120‑1 (MQ=255)
                                                                                                  ttttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                 >  1:1444922/1‑149 (MQ=255)
                                                                                                   tttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtt                                                                                                                                       <  3:1408417/62‑1 (MQ=255)
                                                                                                    ttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCaa                                               <  4:924225/149‑1 (MQ=255)
                                                                                                        tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTgg                                           <  3:1137627/149‑1 (MQ=255)
                                                                                                          tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGAt                                                                                                      <  1:1231377/88‑1 (MQ=255)
                                                                                                           gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                                                   >  3:700053/1‑106 (MQ=255)
                                                                                                             ttGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTc                                                                                                              >  2:693314/1‑77 (MQ=255)
                                                                                                             ttGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTc                                                                                                              <  1:693314/77‑1 (MQ=255)
                                                                                                                tCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                   >  3:23817/1‑53 (MQ=255)
                                                                                                                tCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgt                                                                                                                                   <  4:23817/53‑1 (MQ=255)
                                                                                                                  gCCACTGTTGAAGCTGATTGAGTAAACCGTAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                                                  >  3:830403/1‑132 (MQ=255)
                                                                                                                  gCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                                                  <  4:327659/132‑1 (MQ=255)
                                                                                                                  gCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                                                  >  3:327659/1‑132 (MQ=255)
                                                                                                                   ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTg                                                                             <  2:334347/104‑1 (MQ=255)
                                                                                                                   ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTg                                                                             >  1:334347/1‑104 (MQ=255)
                                                                                                                    cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                                                   >  4:662518/1‑97 (MQ=255)
                                                                                                                    cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAgg                                    <  2:134356/144‑1 (MQ=255)
                                                                                                                    cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAgg                                    >  1:134356/1‑144 (MQ=255)
                                                                                                                        gTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgc                           <  1:992979/149‑1 (MQ=255)
                                                                                                                              gCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgac                     >  2:268700/1‑149 (MQ=255)
                                                                                                                                   ttGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAg                >  4:1220068/1‑149 (MQ=255)
                                                                                                                                       gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                 <  2:1014962/112‑1 (MQ=255)
                                                                                                                                       gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                 >  1:1014962/1‑112 (MQ=255)
                                                                                                                                       gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTg                                            <  4:653639/117‑1 (MQ=255)
                                                                                                                                       gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTg                                            >  3:653639/1‑117 (MQ=255)
                                                                                                                                       caaaaCCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCt                                                   <  2:744554/108‑1 (MQ=255)
                                                                                                                                        tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGAt                                                                                                      <  4:363021/58‑1 (MQ=255)
                                                                                                                                          aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                                                 >  3:180983/1‑77 (MQ=255)
                                                                                                                                             cGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGa      <  1:268700/149‑1 (MQ=255)
                                                                                                                                              ggAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGaa     <  4:532936/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TATTGTGTCGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGC  >  NC_000913/3701696‑3701989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: