Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,747,173 Δ1 bp 7.1% coding (90/1497 nt) lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,747,1730G. 7.1% 730.1 / 49.1 210coding (90/1497 nt)lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (101/93);  new base . (7/8);  total (109/101)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 222 total aligned reads displayed.

TTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCC  >  NC_000913/3747025‑3747320
                                                                                                                                                     |                                                                                                                                                   
ttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAg                                                                                                                                                     >  1:980414/1‑149 (MQ=255)
    aGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGcc                                                                                                                                                 >  2:1347147/1‑149 (MQ=255)
      aTGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCACGCCTg                                                                                                                                               <  1:733590/149‑1 (MQ=255)
       tgtgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGGAGGCCGGGAGGCAGGCGCTGCACGCCTGc                                                                                                                                              <  3:722174/149‑1 (MQ=255)
         tgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCg                                                                                                                                            >  2:572096/1‑149 (MQ=255)
                 ggAGCTGATCTGCTGAAAGCCGTGGCTGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATCACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTgcgc                                                                                                                                    >  4:1448069/1‑149 (MQ=255)
                     cTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTa                                                                                                                                >  3:490114/1‑149 (MQ=255)
                      tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCACGCCTg                                                                                                                                               <  2:166443/133‑1 (MQ=255)
                      tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCACGCCTg                                                                                                                                               >  1:166443/1‑133 (MQ=255)
                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCt                                                                                                                                                <  2:1388292/130‑1 (MQ=255)
                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCt                                                                                                                                                >  1:1388292/1‑130 (MQ=255)
                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCt                                                                                                                                          >  4:1112227/1‑136 (MQ=255)
                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCt                                                                                                                                          <  3:1112227/136‑1 (MQ=255)
                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                                                                                             >  2:703288/1‑149 (MQ=255)
                         tCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAgcg                                                                                                                                                   <  3:1364978/126‑1 (MQ=255)
                         tCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAgcg                                                                                                                                                   >  4:1364978/1‑126 (MQ=255)
                         tCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                                                                                            <  2:998361/149‑1 (MQ=255)
                         tCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                                                                                            <  3:1448069/149‑1 (MQ=255)
                              tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCGGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                                                                                       >  2:655991/1‑149 (MQ=255)
                              tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGACGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                                                                                       >  4:127073/1‑149 (MQ=255)
                              tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGATAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCt                                                                                                                                                <  1:115389/124‑1 (MQ=255)
                               gAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                                                                                      >  1:833024/1‑149 (MQ=255)
                                    ccGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAgcg                                                                                                                                                   <  3:897397/115‑1 (MQ=255)
                                      gTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                                                                               >  2:1169337/1‑149 (MQ=255)
                                       tGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                                                                              >  3:600448/1‑149 (MQ=255)
                                           tGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATAGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                                                                                          <  3:127073/149‑1 (MQ=255)
                                            gAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                                                                                         <  2:1177809/149‑1 (MQ=255)
                                            gAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                                                                                         >  4:1034478/1‑149 (MQ=255)
                                               gTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                                                                                      >  2:1016134/1‑149 (MQ=255)
                                                tGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcgc                                                                                                     >  4:52071/1‑149 (MQ=255)
                                                tGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcgc                                                                                                     >  4:1125400/1‑149 (MQ=255)
                                                  cAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                                                                                   <  3:52071/149‑1 (MQ=255)
                                                   aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                                                                                 <  3:232702/118‑1 (MQ=255)
                                                   aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                                                                                 >  4:232702/1‑118 (MQ=255)
                                                   aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                                  >  2:1271794/1‑149 (MQ=255)
                                                       aaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATgg                                                                                              <  1:256198/149‑1 (MQ=255)
                                                          cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAg                                                                                                                                                     >  1:875284/1‑91 (MQ=255)
                                                             gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                                                                                            >  2:520058/1‑113 (MQ=255)
                                                             gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                                                                                        >  2:1321345/1‑149 (MQ=255)
                                                             gACGCAATACTGGCTGGGGTTAGATTGTGGCAGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                                                                                        >  4:56517/1‑149 (MQ=255)
                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGc                                                                                                                                              >  1:1036430/1‑92 (MQ=255)
                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGc                                                                                                                                              <  2:1036430/92‑1 (MQ=255)
                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTa                                                                                                                                <  4:356277/106‑1 (MQ=255)
                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTa                                                                                                                                >  3:356277/1‑106 (MQ=255)
                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                                                                                            >  3:1211756/1‑110 (MQ=255)
                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                                                                                            >  1:85552/1‑110 (MQ=255)
                                                                 cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                                                                                   <  1:560987/134‑1 (MQ=255)
                                                                 cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                    <  2:833024/149‑1 (MQ=255)
                                                                 cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                    <  1:62413/149‑1 (MQ=255)
                                                                 cAATACTGGCTGGGGTTAGATGGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                    <  1:445170/149‑1 (MQ=255)
                                                                        ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                                                                                      <  4:1035597/124‑1 (MQ=255)
                                                                          ctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctg                                                                           >  3:1251608/1‑149 (MQ=255)
                                                                            ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa                                                                         <  4:490114/149‑1 (MQ=255)
                                                                               gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                                                                              >  2:1140240/1‑109 (MQ=255)
                                                                                ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                                                                                >  2:781559/1‑138 (MQ=255)
                                                                                 tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                    <  1:149853/149‑1 (MQ=255)
                                                                                    aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                                                                              <  2:147713/104‑1 (MQ=255)
                                                                                        tgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                                                                              >  3:431930/1‑100 (MQ=255)
                                                                                        tgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTc                                                             <  4:600448/149‑1 (MQ=255)
                                                                                        tgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTc                                                             <  1:1321345/149‑1 (MQ=255)
                                                                                         ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                            >  2:339570/1‑149 (MQ=255)
                                                                                               aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCt                                                      <  4:1418148/149‑1 (MQ=255)
                                                                                               aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCt                                                      <  1:339570/149‑1 (MQ=255)
                                                                                                 ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                                                                               <  3:1419534/122‑1 (MQ=255)
                                                                                                 ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGc                                                    <  3:56517/149‑1 (MQ=255)
                                                                                                    gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg                                                                              <  3:1072896/120‑1 (MQ=255)
                                                                                                    gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTa                                                 <  1:503321/149‑1 (MQ=255)
                                                                                                     ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                                <  4:874386/149‑1 (MQ=255)
                                                                                                      tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACt                                               >  3:1005639/1‑149 (MQ=255)
                                                                                                       gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                                                                                     <  3:1136052/110‑1 (MQ=255)
                                                                                                       gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                      <  1:1287110/125‑1 (MQ=255)
                                                                                                        aaaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                                   >  4:903291/1‑111 (MQ=255)
                                                                                                         aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGTATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCAt                                            <  4:1005639/149‑1 (MQ=255)
                                                                                                          aGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCAtt                                           <  1:1347147/149‑1 (MQ=255)
                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                          <  1:1016134/149‑1 (MQ=255)
                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCGGTCATTCGCGCCCTGCTTACTCATTc                                          <  4:1251608/149‑1 (MQ=255)
                                                                                                             cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTg                                        <  2:857152/149‑1 (MQ=255)
                                                                                                              gggCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                       <  3:1035051/149‑1 (MQ=255)
                                                                                                                      tGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                              <  2:900809/54‑1 (MQ=255)
                                                                                                                      tGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                                                                               >  4:1168992/1‑101 (MQ=255)
                                                                                                                      tGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCg                               >  3:1376958/1‑149 (MQ=255)
                                                                                                                       gACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                    >  3:923600/1‑111 (MQ=255)
                                                                                                                       gACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                    <  4:923600/111‑1 (MQ=255)
                                                                                                                       gACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                      <  1:1174384/141‑1 (MQ=255)
                                                                                                                        aCCGCGAAGGCCGGGAGGCGGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCggg                             <  1:655991/149‑1 (MQ=255)
                                                                                                                          cgcgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGc                                                    <  4:1140776/124‑1 (MQ=255)
                                                                                                                            cgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAAc                         >  1:120083/1‑149 (MQ=255)
                                                                                                                                   cGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt                  >  3:697428/1‑149 (MQ=255)
                                                                                                                                        ggcaggcGCTGCACGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                                >  1:777777/1‑114 (MQ=255)
                                                                                                                                           aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgtgt                                    >  1:788155/1‑123 (MQ=255)
                                                                                                                                           aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg          >  4:642357/1‑149 (MQ=255)
                                                                                                                                           aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg          >  4:404325/1‑149 (MQ=255)
                                                                                                                                             gcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTc                                                                 >  3:86832/1‑92 (MQ=255)
                                                                                                                                             gcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGc        <  4:1376958/149‑1 (MQ=255)
                                                                                                                                               gTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                      <  4:757686/117‑1 (MQ=255)
                                                                                                                                                 tGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAtc     >  3:1148488/1‑149 (MQ=255)
                                                                                                                                                  gCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                            <  1:1104039/93‑1 (MQ=255)
                                                                                                                                                   cAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAtctc   >  2:788687/1‑149 (MQ=255)
                                                                                                                                                    aGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTcc  >  2:472797/1‑149 (MQ=255)
                                                                                                                                                     gcgcCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt                  <  3:947964/132‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCC  >  NC_000913/3747025‑3747320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: