Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,285,870:1 +G 6.3% coding (1502/1959 nt) acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,285,8701.G 6.3% 414.3 / 22.9 95coding (1502/1959 nt)acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base . (45/44);  new base G (2/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCC  >  NC_000913/4285730‑4286001
                                                                                                                                             |                                                                                                                                   
ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             <  4:248447/149‑3 (MQ=255)
ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             <  1:817861/149‑3 (MQ=255)
              gCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAtt                                                                                                               >  3:438082/1‑149 (MQ=255)
                aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAtttt                                                                                                             >  4:599390/1‑149 (MQ=255)
                aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAtttt                                                                                                             <  2:506317/149‑1 (MQ=255)
                    ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    >  4:302019/1‑138 (MQ=255)
                    ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    <  3:302019/138‑1 (MQ=255)
                    ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGaa                                                                                                         >  4:736531/1‑149 (MQ=255)
                       ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttt                                                                                                              >  2:1310207/1‑141 (MQ=255)
                       ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttt                                                                                                              <  1:1310207/141‑1 (MQ=255)
                           gcgcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGgag                                                                                                  <  4:438082/149‑1 (MQ=255)
                             gcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa                                                                                                >  2:937740/1‑149 (MQ=255)
                                      gcCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTg                                                                                       >  2:525862/1‑149 (MQ=255)
                                              aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc                                                                               >  2:545744/1‑149 (MQ=255)
                                              aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc                                                                               >  4:1455841/1‑149 (MQ=255)
                                               aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGaaa                                                                                                                       <  2:1312513/108‑1 (MQ=255)
                                               aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGaaa                                                                                                                       >  1:1312513/1‑108 (MQ=255)
                                               aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACg                                                                              >  3:1041044/1‑149 (MQ=255)
                                                 ctctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGtt                                                                            <  1:545744/149‑1 (MQ=255)
                                                  tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  3:1455841/149‑1 (MQ=255)
                                                  tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:525862/149‑1 (MQ=255)
                                                  tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  3:702491/1‑149 (MQ=255)
                                                  tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  3:599390/149‑1 (MQ=255)
                                                  tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:937740/149‑1 (MQ=255)
                                                    tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    >  2:366045/1‑106 (MQ=255)
                                                    tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    <  1:366045/106‑1 (MQ=255)
                                                    tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt                                                                         <  3:736531/149‑1 (MQ=255)
                                                      ccGTCCCCAGACGGTGACCGGAGACGTTCAGCCCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             >  3:417100/1‑93 (MQ=255)
                                                      ccGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCTGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGGTCATAACGTTCGTGa                                                                       >  1:1122746/1‑149 (MQ=255)
                                                      ccGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             <  4:417100/95‑3 (MQ=255)
                                                        gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcgat                                                                                                                            >  2:1206719/1‑91 (MQ=255)
                                                        gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcgat                                                                                                                            <  1:1206719/94‑4 (MQ=255)
                                                          ccccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGc                                                                   <  4:1041044/149‑1 (MQ=255)
                                                            ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTTAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGc                                                                   <  1:958812/147‑1 (MQ=255)
                                                            ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt                                                                                                      <  4:1064004/112‑1 (MQ=255)
                                                            ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt                                                                                                      >  3:1064004/1‑112 (MQ=255)
                                                            ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGc                                                                   >  2:958812/1‑147 (MQ=255)
                                                            ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa                                                                 >  2:819310/1‑149 (MQ=255)
                                                            ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa                                                                 >  3:533888/1‑149 (MQ=255)
                                                                  ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGc                                                           <  2:1122746/149‑1 (MQ=255)
                                                                      aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAc                                                       >  2:1180548/1‑149 (MQ=255)
                                                                      aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAc                                                       >  3:614943/1‑149 (MQ=255)
                                                                         ggAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgcg                                                    >  4:1220526/1‑149 (MQ=255)
                                                                          gagaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc                                                   <  1:1180548/149‑1 (MQ=255)
                                                                             aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttttt                                                                                                            >  1:494426/1‑89 (MQ=255)
                                                                             aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttttt                                                                                                            <  2:494426/89‑1 (MQ=255)
                                                                             aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTg                                                >  1:467895/1‑149 (MQ=255)
                                                                               gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAc                                              >  2:175207/1‑149 (MQ=255)
                                                                                ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                     <  4:291522/109‑1 (MQ=255)
                                                                                ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                     >  3:291522/1‑109 (MQ=255)
                                                                                ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAcc                                             >  2:1239275/1‑149 (MQ=255)
                                                                                    gCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc                                                   >  3:175752/1‑139 (MQ=255)
                                                                                    gCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc                                                   <  4:175752/139‑1 (MQ=255)
                                                                                     cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        >  2:337395/1‑149 (MQ=255)
                                                                                     cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        >  4:1431155/1‑149 (MQ=255)
                                                                                     cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        <  4:533888/149‑1 (MQ=255)
                                                                                      aCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:1276399/113‑1 (MQ=255)
                                                                                      aCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  2:1276399/1‑113 (MQ=255)
                                                                                      aCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCa                                       <  4:702491/149‑1 (MQ=255)
                                                                                         tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                     <  2:223140/84‑1 (MQ=255)
                                                                                         tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                     >  1:223140/1‑84 (MQ=255)
                                                                                            tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc                                 <  1:819310/149‑1 (MQ=255)
                                                                                              cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCgg                               <  3:1220526/149‑1 (MQ=255)
                                                                                                cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTg                             >  2:476974/1‑149 (MQ=255)
                                                                                                 acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                            <  3:1431155/149‑1 (MQ=255)
                                                                                                 acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                            <  4:614943/149‑1 (MQ=255)
                                                                                                 acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                            <  1:1239275/149‑1 (MQ=255)
                                                                                                   gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                          <  1:476974/149‑1 (MQ=255)
                                                                                                    cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                         <  2:833458/149‑1 (MQ=255)
                                                                                                     ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  4:984213/1‑98 (MQ=255)
                                                                                                     ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  3:984213/98‑1 (MQ=255)
                                                                                                        gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgc                                                     >  4:389064/1‑117 (MQ=255)
                                                                                                        gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgc                                                     <  3:389064/117‑1 (MQ=255)
                                                                                                             ccAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAc                >  3:812954/1‑149 (MQ=255)
                                                                                                                    tAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt         <  1:337395/149‑1 (MQ=255)
                                                                                                                    tAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt         <  4:812954/149‑1 (MQ=255)
                                                                                                                    tAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt         <  1:175207/149‑1 (MQ=255)
                                                                                                                     aGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgcg                                                    >  4:1029204/1‑105 (MQ=255)
                                                                                                                     aGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgcg                                                    <  3:1029204/105‑1 (MQ=255)
                                                                                                                        cATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                          <  4:1292667/128‑1 (MQ=255)
                                                                                                                        cATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                          >  3:1292667/1‑128 (MQ=255)
                                                                                                                          tCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc   >  1:17110/1‑149 (MQ=255)
                                                                                                                           cTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGccccc  >  3:486641/1‑149 (MQ=255)
                                                                                                                              cATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                     >  3:634770/1‑127 (MQ=255)
                                                                                                                              cATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                     <  4:634770/127‑1 (MQ=255)
                                                                                                                                tCGCGCCGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc                        <  2:56995/122‑1 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  2:995918/1‑71 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:995918/71‑1 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc                        >  1:56995/1‑122 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTg        <  3:855005/138‑1 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTg        >  4:855005/1‑138 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc     >  2:934662/1‑141 (MQ=255)
                                                                                                                                tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc     <  1:934662/141‑1 (MQ=255)
                                                                                                                                 cgcgACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGc                                         >  3:1403600/1‑104 (MQ=255)
                                                                                                                                 cgcgACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGc                                         <  4:1403600/104‑1 (MQ=255)
                                                                                                                                            c‑cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        >  3:1028595/1‑94 (MQ=255)
                                                                                                                                            c‑cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        <  4:1028595/94‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                   
CTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCC  >  NC_000913/4285730‑4286001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑