Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 808,000:1 +A 5.3% coding (1258/1290 nt) bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913808,0001.A 5.3% 331.0 / 13.1 76coding (1258/1290 nt)bioA7,8‑diaminopelargonic acid synthase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base . (37/35);  new base A (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

TGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGCGCCGCCATATTCACCGGATGAGTG  >  NC_000913/807857‑808148
                                                                                                                                                |                                                                                                                                                    
tGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGt                                                                                                                                                 >  4:235214/1‑149 (MQ=255)
         ggCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCAGCGGTTAACCGcg                                                                                                                                         >  1:604966/1‑148 (MQ=255)
         ggCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTg                                                                                                                                        <  3:235214/149‑1 (MQ=255)
         ggCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGACCGCAGCGGTTAACCGcgc                                                                                                                                        >  4:500867/1‑147 (MQ=255)
             aTTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCAGCGGTTAACCGCGCGGt                                                                                                                                    <  2:604966/149‑1 (MQ=255)
               ttATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCa                                                                                                                                  <  3:228815/149‑1 (MQ=255)
                  tCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGACCGCAGCGGTTAACCGCGCGGtacagga                                                                                                                              <  3:500867/149‑7 (MQ=255)
                     ccATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCa                                                                                                                                  >  3:362160/1‑143 (MQ=255)
                     ccATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCa                                                                                                                                  <  4:362160/143‑1 (MQ=255)
                        tCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCgg                                                                                                                                                  >  4:1051475/1‑124 (MQ=255)
                        tCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCgg                                                                                                                                                  <  3:1051475/124‑1 (MQ=255)
                                    ttACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCa                                                                                                                                  <  4:387944/128‑1 (MQ=255)
                                    ttACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCa                                                                                                                                  >  3:387944/1‑128 (MQ=255)
                                     tACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCgg                                                                                                                                                  <  2:1319310/111‑1 (MQ=255)
                                     tACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCgg                                                                                                                                                  >  1:1319310/1‑111 (MQ=255)
                                          atgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAAcc                                                                                                                                            <  1:313671/112‑1 (MQ=255)
                                          atgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAAcc                                                                                                                                            >  2:313671/1‑112 (MQ=255)
                                                     gAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaa                                                                                                          >  4:1250155/1‑135 (MQ=255)
                                                     gAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaa                                                                                                          <  3:1250155/135‑1 (MQ=255)
                                                         cctcctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc                                                                                                                                       >  2:1169333/1‑102 (MQ=255)
                                                         cctcctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc                                                                                                                                       <  1:1169333/102‑1 (MQ=255)
                                                         cctcctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaata                                                                                                        >  2:1308123/1‑133 (MQ=255)
                                                         cctcctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaata                                                                                                        <  1:1308123/133‑1 (MQ=255)
                                                           tcctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTc                                                                                                                                   >  3:860467/1‑104 (MQ=255)
                                                           tcctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTc                                                                                                                                   <  4:860467/104‑1 (MQ=255)
                                                             ctTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTa                                                                                    >  3:809840/1‑149 (MQ=255)
                                                              tttCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc                                                                                                                                       <  4:477831/97‑1 (MQ=255)
                                                              tttCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc                                                                                                                                       >  3:477831/1‑97 (MQ=255)
                                                              tttCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTaa                                                                                   >  3:587044/1‑149 (MQ=255)
                                                               ttCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTaaa                                                                                  >  3:1216979/1‑149 (MQ=255)
                                                                       tGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGc                                                                                                                          <  3:655881/101‑1 (MQ=255)
                                                                       tGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGc                                                                                                                          >  4:655881/1‑101 (MQ=255)
                                                                         cAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCg                                                                                                                                      >  3:207988/1‑87 (MQ=255)
                                                                         cAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCg                                                                                                                                      <  4:207988/87‑1 (MQ=255)
                                                                          aGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGt                                                                             <  3:1033732/143‑1 (MQ=255)
                                                                          aGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGt                                                                             >  4:1033732/1‑143 (MQ=255)
                                                                          aGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCa                                                                       >  1:312836/1‑149 (MQ=255)
                                                                           gAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCaa                                                                      >  1:1215090/1‑149 (MQ=255)
                                                                            aaaGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGt                                                                             <  3:972605/141‑1 (MQ=255)
                                                                            aaaGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGt                                                                             >  4:972605/1‑141 (MQ=255)
                                                                              aGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAg                                                                   <  4:1216979/149‑1 (MQ=255)
                                                                                tgtAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGc                                                                 <  4:587044/149‑1 (MQ=255)
                                                                                  tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCg                                                               >  3:1315139/1‑149 (MQ=255)
                                                                                    gCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGa                                                             <  2:312836/149‑1 (MQ=255)
                                                                                      cAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATc                                                           >  4:83734/1‑149 (MQ=255)
                                                                                              cTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAg                                                                                                  >  2:919530/1‑102 (MQ=255)
                                                                                              cTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAg                                                                                                  <  3:487909/102‑1 (MQ=255)
                                                                                              cTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAg                                                                                                  >  4:487909/1‑102 (MQ=255)
                                                                                              cTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAg                                                                                                  <  1:919530/102‑1 (MQ=255)
                                                                                               tCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGc                                                                                                                             <  2:738965/74‑1 (MQ=255)
                                                                                               tCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGc                                                                                                                             >  1:738965/1‑74 (MQ=255)
                                                                                               tCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAg                                                        <  1:1016088/143‑1 (MQ=255)
                                                                                               tCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAg                                                        >  2:1016088/1‑143 (MQ=255)
                                                                                                 aCGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTg                                                >  2:1398913/1‑149 (MQ=255)
                                                                                                   gcgGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGtt                                              <  4:1315139/149‑1 (MQ=255)
                                                                                                    cgGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTc                                             <  2:1215090/149‑1 (MQ=255)
                                                                                                     ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                                                                           <  1:1324691/118‑1 (MQ=255)
                                                                                                     ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                                                                           <  4:595954/118‑1 (MQ=255)
                                                                                                     ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                                                                           >  4:1136706/1‑118 (MQ=255)
                                                                                                     ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                                                                           >  3:595954/1‑118 (MQ=255)
                                                                                                     ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                                                                           <  3:1136706/118‑1 (MQ=255)
                                                                                                     ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                                                                           >  2:1324691/1‑118 (MQ=255)
                                                                                                       aCTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGAc                                          <  1:1398913/149‑1 (MQ=255)
                                                                                                         ttCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCAt                                                                                          >  3:627206/1‑99 (MQ=255)
                                                                                                         ttCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCAt                                                                                          <  4:627206/99‑1 (MQ=255)
                                                                                                            tcGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaataa                                                                                                       <  1:1074302/83‑1 (MQ=255)
                                                                                                            tcGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaataa                                                                                                       >  2:1074302/1‑83 (MQ=255)
                                                                                                             cGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGaa                                    <  3:83734/149‑1 (MQ=255)
                                                                                                              gTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAAt                                   <  4:809840/149‑1 (MQ=255)
                                                                                                                        aaaaaaTGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAgcg                         >  3:982734/1‑149 (MQ=255)
                                                                                                                            aaTGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGcgccgc                     >  2:170585/1‑149 (MQ=255)
                                                                                                                                    aTCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGCGCCGCCATATTCa             >  1:65653/1‑149 (MQ=255)
                                                                                                                                     tCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGCGCCGCCATATTCAc            >  3:528329/1‑149 (MQ=255)
                                                                                                                                      ccTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAAttt                                 <  2:1152047/127‑1 (MQ=255)
                                                                                                                                      ccTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAAttt                                 >  1:1152047/1‑127 (MQ=255)
                                                                                                                                          tACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGCGCCGCCATATTCACCGGAt       <  4:528329/149‑1 (MQ=255)
                                                                                                                                              gcgcgGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAAttttt                               >  1:1400760/1‑121 (MQ=255)
                                                                                                                                              gcgcgGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAAttttt                               <  2:1400760/121‑1 (MQ=255)
                                                                                                                                               cgcgGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGCGCCGCCATATTCACCGGATGAGTg  <  2:65653/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCCTGTTCGACAAAGAATTTTTGCAGCGCCGCCATATTCACCGGATGAGTG  >  NC_000913/807857‑808148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: