Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,799,374:1 +T 12.0% intergenic (‑104/+19) pheM ← / ← rplT phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,3741.T12.0% 308.8 / 44.9 83intergenic (‑104/+19)pheM/rplTphenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20
Reads supporting (aligned to +/- strand):  ref base . (38/35);  new base T (5/5);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

AGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGC  >  NC_000913/1799237‑1799520
                                                                                                                                          |                                                                                                                                                  
aGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                         >  4:538356/1‑149 (MQ=255)
                 aaaaTAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCATAAGCCagt                                                                                                                        <  3:538356/149‑2 (MQ=255)
                     tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCt                                                                                                                                                  <  3:263044/119‑1 (MQ=255)
                     tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCt                                                                                                                                                  >  4:263044/1‑119 (MQ=255)
                                      tgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAcc                                                                                                   <  2:1303339/149‑1 (MQ=255)
                                          ttACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCagag                                                                                               >  4:351105/1‑149 (MQ=255)
                                              cTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCaga                                                                                                                        <  1:842471/120‑1 (MQ=255)
                                              cTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCaga                                                                                                                        >  2:842471/1‑120 (MQ=255)
                                               ttttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAc                                                                                                    >  1:195742/1‑139 (MQ=255)
                                               ttttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAc                                                                                                    <  2:195742/139‑1 (MQ=255)
                                                    gTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                         <  2:424588/97‑1 (MQ=255)
                                                    gTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                         >  1:424588/1‑97 (MQ=255)
                                                    gTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAAc                                                                                                                                      <  3:39199/100‑1 (MQ=255)
                                                    gTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAAc                                                                                                                                      >  4:39199/1‑100 (MQ=255)
                                                     tACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCt                                                                                    >  1:1154335/1‑149 (MQ=255)
                                                      aCTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt                                                                                                         <  3:417590/127‑1 (MQ=255)
                                                      aCTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt                                                                                                         >  4:417590/1‑127 (MQ=255)
                                                        tctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                         >  4:990467/1‑93 (MQ=255)
                                                        tctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                         <  3:990467/93‑1 (MQ=255)
                                                          ttCAAAAGACCTTTATTGAACAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGc                                                                                                                            <  4:834233/104‑1 (MQ=255)
                                                          ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGc                                                                                                                            >  3:834233/1‑104 (MQ=255)
                                                          ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAAc                                                                                       <  1:773312/141‑1 (MQ=255)
                                                          ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAAc                                                                                       >  2:773312/1‑141 (MQ=255)
                                                          ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                               >  1:1322796/1‑149 (MQ=255)
                                                             aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGc                                                                                              <  4:57431/131‑1 (MQ=255)
                                                             aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGc                                                                                              >  3:57431/1‑131 (MQ=255)
                                                             aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            <  3:351105/149‑1 (MQ=255)
                                                              aaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTg                                                                                                                                    <  3:154217/92‑1 (MQ=255)
                                                              aaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt                                                                                                         >  2:605212/1‑119 (MQ=255)
                                                              aaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt                                                                                                         <  1:605212/119‑1 (MQ=255)
                                                              aaaGACCTTTATTGAAAAGCCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTg                                                                                                                                    >  4:154217/1‑92 (MQ=255)
                                                                  aCCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            <  4:1351292/144‑1 (MQ=255)
                                                                  aCCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            >  3:1351292/1‑144 (MQ=255)
                                                                    cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttc                                                                                                                                        >  1:1489/1‑80 (MQ=38)
                                                                    cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttc                                                                                                                                        <  2:1489/82‑3 (MQ=38)
                                                                         ttGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCg                                                                                             >  3:270300/1‑120 (MQ=255)
                                                                         ttGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCg                                                                                             <  4:270300/120‑1 (MQ=255)
                                                                           gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt                                                                                           >  3:301202/1‑120 (MQ=255)
                                                                           gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt                                                                                           <  4:301202/120‑1 (MQ=255)
                                                                           gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACt                                                                     >  3:230554/1‑142 (MQ=255)
                                                                           gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACt                                                                     <  4:230554/142‑1 (MQ=255)
                                                                            aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            >  2:99374/1‑134 (MQ=255)
                                                                            aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            <  1:99374/134‑1 (MQ=255)
                                                                            aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc                                                             >  1:1336092/1‑149 (MQ=255)
                                                                                ggCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGcc                                                         >  2:740100/1‑149 (MQ=255)
                                                                                   tACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                             >  1:402782/1‑126 (MQ=255)
                                                                                   tACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                             <  2:402782/126‑1 (MQ=255)
                                                                                     cGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGAt                                                    <  2:1154335/149‑1 (MQ=255)
                                                                                         gATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTa                                                >  3:410025/1‑149 (MQ=255)
                                                                                             aaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGa                                                                 >  3:955088/1‑128 (MQ=255)
                                                                                             aaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGa                                                                 <  4:955088/128‑1 (MQ=255)
                                                                                             aaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGt                                            <  2:1322796/149‑1 (MQ=255)
                                                                                              aaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGc                                                                                                                            >  4:590723/1‑68 (MQ=255)
                                                                                              aaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGc                                                                                                                            <  3:590723/68‑1 (MQ=255)
                                                                                                gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGa                                         <  4:410025/149‑1 (MQ=255)
                                                                                                   aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACg                                              <  3:1201919/141‑1 (MQ=255)
                                                                                                   aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACg                                              >  4:1201919/1‑141 (MQ=255)
                                                                                                           gATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACagag                              <  1:740100/149‑1 (MQ=255)
                                                                                                             tGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttt                                                                                                          <  3:384952/71‑1 (MQ=255)
                                                                                                             tGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttt                                                                                                          >  4:384952/1‑71 (MQ=255)
                                                                                                                 gTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACt                                                                                 <  4:173715/92‑1 (MQ=255)
                                                                                                                 gTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACt                                                                                 >  3:173715/1‑92 (MQ=255)
                                                                                                                     aaaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACag                                >  1:264417/1‑137 (MQ=255)
                                                                                                                     aaaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACag                                <  2:264417/137‑1 (MQ=255)
                                                                                                                       aCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                               >  4:728324/1‑88 (MQ=255)
                                                                                                                       aCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                               <  3:728324/88‑1 (MQ=255)
                                                                                                                          aaaaGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa               <  2:1336092/149‑1 (MQ=255)
                                                                                                                           aaaGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCAt              >  2:630292/1‑149 (MQ=255)
                                                                                                                           aaaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            <  3:199839/87‑1 (MQ=38)
                                                                                                                           aaaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                            >  4:199839/1‑87 (MQ=38)
                                                                                                                            aaGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCAtt             >  3:1283250/1‑149 (MQ=255)
                                                                                                                            aaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCg                                                                                             >  4:1394838/1‑69 (MQ=37)
                                                                                                                            aaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCg                                                                                             <  3:1394838/69‑1 (MQ=37)
                                                                                                                            aaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCt                                                  >  2:375786/1‑112 (MQ=255)
                                                                                                                            aaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCt                                                  <  1:375786/112‑1 (MQ=255)
                                                                                                                             agagGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtg            >  1:1215914/1‑149 (MQ=255)
                                                                                                                             agagGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAg                   >  2:483229/1‑142 (MQ=255)
                                                                                                                             agagGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAg                   <  1:483229/142‑1 (MQ=255)
                                                                                                                               agGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               >  3:551791/1‑128 (MQ=255)
                                                                                                                               agGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                               <  4:551791/128‑1 (MQ=255)
                                                                                                                                 ggAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTa                                                <  2:153102/109‑1 (MQ=255)
                                                                                                                                 ggAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTa                                                >  1:153102/1‑109 (MQ=255)
                                                                                                                                       tAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGc  >  4:716521/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
AGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGC  >  NC_000913/1799237‑1799520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: