Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,016:1 +C 10.1% intergenic (+52/+32) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0161.C10.1% 322.3 / 34.2 79intergenic (+52/+32)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base . (38/33);  new base C (4/4);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

TATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCC  >  NC_000913/2389878‑2390163
                                                                                                                                           |                                                                                                                                                    
tATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                           >  3:453350/1‑149 (MQ=255)
  tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTc                                                                                                                                         <  3:1315250/149‑1 (MQ=255)
       ttAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACt                                                                                                                                    <  4:453350/149‑1 (MQ=255)
                aTGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                     >  2:222664/1‑139 (MQ=255)
                aTGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                     <  1:222664/139‑1 (MQ=255)
                                     ggCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCAtt                                                                                                      >  4:920575/1‑149 (MQ=255)
                                          ttATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCaa                                                                                                 >  4:650233/1‑149 (MQ=255)
                                                 tAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCaaa                                                                                                <  1:335541/143‑1 (MQ=255)
                                                 tAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCaaa                                                                                                >  2:335541/1‑143 (MQ=255)
                                                      tATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTc                                                                                                                                                    <  1:1056367/87‑1 (MQ=255)
                                                      tATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTc                                                                                                                                                    >  2:1056367/1‑87 (MQ=255)
                                                       aTTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTa                                                                                    <  3:650233/149‑1 (MQ=255)
                                                                   gTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgt                                                                        <  3:920575/149‑1 (MQ=255)
                                                                       tACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAAc                                                                                               >  3:208086/1‑121 (MQ=255)
                                                                       tACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAAc                                                                                               <  4:208086/121‑1 (MQ=255)
                                                                             gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTa                                                                                                                   <  1:169943/96‑1 (MQ=255)
                                                                             gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTa                                                                                                                   >  2:169943/1‑96 (MQ=255)
                                                                              ttCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACAcc                                                             >  2:235461/1‑149 (MQ=255)
                                                                              ttCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGACATGCTcggcgg                                                                                                                                               <  3:83011/68‑1 (MQ=38)
                                                                              ttCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGACATGCTcggcgg                                                                                                                                               >  4:83011/1‑68 (MQ=38)
                                                                                 aCTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAg                                                          >  1:977241/1‑149 (MQ=255)
                                                                                   ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTAc                                                                                                                  >  3:825635/1‑91 (MQ=255)
                                                                                   ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTAc                                                                                                                  <  4:825635/91‑1 (MQ=255)
                                                                                     aaCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                      >  1:379712/1‑149 (MQ=255)
                                                                                        gCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACaa                                                                                         >  1:474327/1‑111 (MQ=255)
                                                                                        gCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACaa                                                                                         <  2:474327/111‑1 (MQ=255)
                                                                                                  tatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                       <  2:1432272/119‑1 (MQ=255)
                                                                                                  tatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                       >  1:1432272/1‑119 (MQ=255)
                                                                                                   atctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGTATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAc                                                       >  4:1386599/1‑134 (MQ=255)
                                                                                                   atctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAc                                                       <  3:1386599/134‑1 (MQ=255)
                                                                                                    tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGAtt                                                                                                                    <  2:1270506/72‑1 (MQ=255)
                                                                                                    tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGAtt                                                                                                                    >  1:1270506/1‑72 (MQ=255)
                                                                                                    tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCt                                                                                     <  2:1322384/103‑1 (MQ=255)
                                                                                                    tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCt                                                                                     >  1:1322384/1‑103 (MQ=255)
                                                                                                      tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCa                                                     <  4:658523/133‑1 (MQ=255)
                                                                                                      tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCa                                                     >  3:658523/1‑133 (MQ=255)
                                                                                                       actaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAc                                                       >  4:303244/1‑130 (MQ=255)
                                                                                                       actaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAc                                                       <  3:303244/130‑1 (MQ=255)
                                                                                                       actaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCagag                                    >  2:961508/1‑149 (MQ=255)
                                                                                                         taTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                  >  4:1228679/1‑149 (MQ=255)
                                                                                                         taTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGACGGCTTTCTGCCTTACAAAGTACCAg                                                                                                                       <  2:695808/64‑1 (MQ=255)
                                                                                                         taTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGACGGCTTTCTGACTTACAAAGTAACAg                                                                                                                       >  1:695808/1‑64 (MQ=255)
                                                                                                          aTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATc                                 <  1:961508/149‑1 (MQ=255)
                                                                                                           tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                                >  1:1444487/1‑149 (MQ=255)
                                                                                                           tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                                <  2:977241/149‑1 (MQ=255)
                                                                                                           tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                                <  1:235461/149‑1 (MQ=255)
                                                                                                            cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                               <  2:1444487/149‑1 (MQ=255)
                                                                                                              cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTAcc                                                >  2:702959/1‑130 (MQ=255)
                                                                                                              cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTAcc                                                <  1:702959/130‑1 (MQ=255)
                                                                                                              cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                             >  2:117384/1‑149 (MQ=255)
                                                                                                               ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGc                                                                                                            <  2:67676/69‑1 (MQ=255)
                                                                                                               ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGc                                                                                                            >  1:67676/1‑69 (MQ=255)
                                                                                                               ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACAccc                                                            <  1:265141/117‑1 (MQ=255)
                                                                                                               ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACAccc                                                            >  2:265141/1‑117 (MQ=255)
                                                                                                                  tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                  <  3:45718/140‑1 (MQ=255)
                                                                                                                  tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                  >  4:45718/1‑140 (MQ=255)
                                                                                                                     ttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCAt                                                                  >  1:754504/1‑105 (MQ=255)
                                                                                                                     ttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCAt                                                                  <  2:754504/105‑1 (MQ=255)
                                                                                                                        aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAcc                   >  1:1360502/1‑149 (MQ=255)
                                                                                                                         gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                  >  3:282643/1‑149 (MQ=255)
                                                                                                                         agaaagccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                  <  3:1067709/128‑1 (MQ=38)
                                                                                                                         agaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                                  >  4:1067709/3‑134 (MQ=38)
                                                                                                                         agaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                              <  4:521363/136‑1 (MQ=38)
                                                                                                                         agaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                              >  3:521363/3‑138 (MQ=38)
                                                                                                                           aaaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcc                >  3:1244748/1‑149 (MQ=255)
                                                                                                                            aaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc               >  2:1182234/1‑149 (MQ=255)
                                                                                                                            aaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc               <  4:282643/149‑1 (MQ=255)
                                                                                                                             agccgccgACATGCTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACa                                            >  2:1330250/3‑119 (MQ=255)
                                                                                                                             agccgccgACATGCTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACa                                            <  1:1330250/117‑1 (MQ=255)
                                                                                                                             aaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccg              >  3:131805/1‑149 (MQ=255)
                                                                                                                                cgccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                               >  1:813711/1‑129 (MQ=255)
                                                                                                                                cgccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                               <  2:813711/129‑1 (MQ=255)
                                                                                                                                    gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGa                                                                                                                      >  4:1076005/1‑38 (MQ=255)
                                                                                                                                    gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGa                                                                                                                      <  3:1076005/38‑1 (MQ=255)
                                                                                                                                     aGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAg                                                    <  1:781653/103‑1 (MQ=255)
                                                                                                                                     aGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAg                                                    >  2:781653/1‑103 (MQ=255)
                                                                                                                                      gCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACt     <  1:1182234/149‑1 (MQ=255)
                                                                                                                                        aTGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCCGTACCAACAGTGCAGAGCTCAGCACAACGATACCGCCCGCGCTGTACTGc   >  4:1064620/1‑149 (MQ=255)
                                                                                                                                         tGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGcc  <  4:1244748/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCC  >  NC_000913/2389878‑2390163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: