New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4379180 =	111 (0.800)	4 (0.030)	4/220	NT	3.3%	coding (187/360 nt)	frdD	fumarate reductase (anaerobic), membrane anchor subunit
	?	NC_000913	4379227 =	124 (0.920)					coding (140/360 nt)	frdD	fumarate reductase (anaerobic), membrane anchor subunit
Rejected: Frequency below/above cutoff threshold.

ATTCTGGGGCCTCTTCGGGGCCGGTGGTATGTGGAGCGCCATCATTGCGCCGGTGATGATCCTGCTGGTGGGTATTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4379328‑4379180 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcaCCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCT  >  NC_000913/4379227‑4379374                                                                                                                                                                                                                                                                                                           ATTCTGGGGCCTCTTCGGGGCCGGTGGTATGTGGAGCGCCATCATTGCGCCGGTGATGATCCTGCTGGTGGGTATTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCA                                                                                                                                                      <  4:5022/149‑1                CGGGGCCGGTGGTATGTGGAGCGCCATCATTGCGCCGGTGATGATCCTGCTGGTGGGTATTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGTGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTC                                                                                                                                                       <  4:851274/133‑1                CGGGGCCGGTGGTATGTGGAGCGCCATCATTGCGCCGGTGATGATCCTGCTGGTGGGTATACTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTC                                                                                                                                                       >  3:851274/1‑133                                                               TGCTGGTGGGTATTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTT                                                                                                                                                        >  3:365450/1‑85                                                               TGCTGGTGGGTATTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTT                                                                                                                                                        <  4:365450/85‑1                                                                           TTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTC                                                                                                                                                       <  3:957645/74‑1                                                                           TTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTC                                                                                                                                                       >  4:957645/1‑74                                                                                                       TGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAG                                                   <  3:1130806/146‑1                                                                                                       TGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAG                                                   >  4:1130806/1‑146                                                                                                        GATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATG                                                                                       >  1:110740/1‑109                                                                                                        GATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATG                                                                                       <  2:110740/109‑1                                                                                                                                                    CACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGT                                      <  3:800932/114‑1                                                                                                                                                    CACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGT                                      >  4:800932/1‑114                                                                                                                                                     ACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCT  >  1:857781/1‑149                                                                                                                                                                                                                                                                                                           ATTCTGGGGCCTCTTCGGGGCCGGTGGTATGTGGAGCGCCATCATTGCGCCGGTGATGATCCTGCTGGTGGGTATTCTGCTGCCACTGGGGTTGTTTCCGGGTGATGCGCTGAGCTACGAGCGCGTTCTGGCGTTCGCGCAGAGCTTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4379328‑4379180 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcaCCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCT  >  NC_000913/4379227‑4379374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.