Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,378,285 T→A 11.1% K123* (AAA→TAA) ‡ rplM ← 50S ribosomal subunit protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,378,2850TA11.1% 575.6 / 49.5 179K123* (AAA→TAA) ‡rplM50S ribosomal subunit protein L13
Reads supporting (aligned to +/- strand):  ref base T (83/76);  new base A (10/10);  total (93/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 190 total aligned reads displayed.

CCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTA  >  NC_000913/3378138‑3378432
                                                                                                                                                   |                                                                                                                                                   
ccGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTc                                                                                                                                                    <  2:980366/149‑1 (MQ=255)
                     cTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTc                                                                                                                                                    <  2:1383953/128‑1 (MQ=255)
                       gCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGAcc                                                                                                                             <  1:1202706/149‑1 (MQ=255)
                         ggAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACttt                                                                                                                                                     >  2:646413/1‑123 (MQ=255)
                          gAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTGAAAGTTTACg                                                                                                                                            <  3:965691/131‑1 (MQ=255)
                            aCTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAg                                                                                                                          >  3:703635/1‑147 (MQ=255)
                            aCTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCg                                                                                                                        <  3:1172344/149‑1 (MQ=255)
                              ttttGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCa                                                                                                                                                   <  3:982549/120‑1 (MQ=255)
                              ttttGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGaa                                                                                                                                         <  2:1232422/130‑1 (MQ=255)
                                            gTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCtttt                                                                                                                <  1:162178/141‑1 (MQ=255)
                                            gTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCtttt                                                                                                                >  2:162178/1‑141 (MQ=255)
                                                      aTTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGaa                                                                                                                                         <  1:1430970/106‑1 (MQ=255)
                                                       ttGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCAc                                                                                                                                 <  2:150422/113‑1 (MQ=255)
                                                       ttGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTCAACcg                                                                                             >  2:1130701/1‑149 (MQ=255)
                                                         gATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCtt                                                                                                   <  2:279234/141‑1 (MQ=255)
                                                         gATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCtt                                                                                                   >  1:279234/1‑141 (MQ=255)
                                                            tttCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGAtt                                                                                        >  3:1227730/1‑149 (MQ=255)
                                                                   cATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATca                                                                                 >  3:161401/1‑149 (MQ=255)
                                                                    aTTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATcac                                                                                >  4:27966/1‑149 (MQ=255)
                                                                         cTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGAt                                                                                         <  1:765071/135‑1 (MQ=255)
                                                                         cTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGAt                                                                                         >  2:765071/1‑135 (MQ=255)
                                                                         cTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATcac                                                                                <  2:247898/144‑1 (MQ=255)
                                                                          tataATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCtttt                                                                                                                <  1:1010655/111‑1 (MQ=255)
                                                                          tataATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCttt                                                                                                                 <  4:1083310/110‑1 (MQ=255)
                                                                          tataATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCttt                                                                                                                 >  3:1083310/1‑110 (MQ=255)
                                                                          tataATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTg                                                                                                               <  1:1338331/112‑1 (MQ=255)
                                                                               tCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTg                                                                                                               >  3:808174/1‑107 (MQ=255)
                                                                               tCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTg                                                                                                               <  4:808174/107‑1 (MQ=255)
                                                                               tCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCaa                                                                                    >  4:388785/1‑134 (MQ=255)
                                                                               tCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGAc                                                                     >  3:1427991/1‑149 (MQ=255)
                                                                                cccGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACg                                                                    >  1:1052159/1‑149 (MQ=255)
                                                                                cccGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACg                                                                    >  2:477794/1‑149 (MQ=255)
                                                                                 ccGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGtt                                                                                                                                                <  4:960514/72‑1 (MQ=255)
                                                                                 ccGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACcgcg                                                                                           >  2:1001499/1‑125 (MQ=255)
                                                                                 ccGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACcgcg                                                                                           <  1:1001499/125‑1 (MQ=255)
                                                                                   gATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCttt                                                                                                  >  1:72450/1‑116 (MQ=255)
                                                                                    aTTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGa                                                                <  1:477794/149‑1 (MQ=255)
                                                                                       aGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCa                                                             <  4:161401/149‑1 (MQ=255)
                                                                                        gATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCaa                                                            >  1:927522/1‑149 (MQ=255)
                                                                                        gATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCaa                                                            >  4:758887/1‑149 (MQ=255)
                                                                                          tGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAatc                                                          <  2:1018945/149‑1 (MQ=255)
                                                                                            tCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCt                                                                                                                   <  1:621835/90‑1 (MQ=255)
                                                                                            tCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGCCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAatcat                                                        >  1:251732/1‑149 (MQ=255)
                                                                                             cAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAatcatc                                                       >  3:601143/1‑149 (MQ=255)
                                                                                              aaGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCaa                                                                                                           <  1:1146884/96‑1 (MQ=255)
                                                                                              aaGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCaa                                                                                                           >  2:1146884/1‑96 (MQ=255)
                                                                                              aaGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCAtct                                                      >  2:96476/1‑149 (MQ=255)
                                                                                              aaGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTGAAAGTTTACgc                                                                                                                                           <  2:887820/64‑2 (MQ=38)
                                                                                              aaGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTGAAAGTTTACgc                                                                                                                                           >  1:887820/1‑63 (MQ=38)
                                                                                              aaGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTATCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGATGGCGAGCAATCAtct                                                      >  2:423774/1‑149 (MQ=255)
                                                                                               aGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCaa                                                                                    <  3:743269/118‑1 (MQ=255)
                                                                                               aGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCaa                                                                                    >  4:743269/1‑118 (MQ=255)
                                                                                                 aaCTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCtt                                                   <  3:758887/149‑1 (MQ=255)
                                                                                                  aCTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGCTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTc                                                  <  4:965882/149‑1 (MQ=255)
                                                                                                   cTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCt                                                                                                                   >  1:348094/1‑83 (MQ=255)
                                                                                                   cTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCt                                                                                                                   <  2:348094/83‑1 (MQ=255)
                                                                                                    ttGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACa                                                                                                         <  3:1137333/92‑1 (MQ=255)
                                                                                                    ttGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACa                                                                                                         >  4:1137333/1‑92 (MQ=255)
                                                                                                    ttGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACa                                                                                                         <  1:650878/92‑1 (MQ=255)
                                                                                                     tGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCaaa                                               <  4:601143/149‑1 (MQ=255)
                                                                                                               gtgCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGt                                     <  3:27966/149‑1 (MQ=255)
                                                                                                                    gcgTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCaa                                                                                    <  2:1128967/97‑1 (MQ=255)
                                                                                                                    gcgTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGAt                                <  2:927522/149‑1 (MQ=255)
                                                                                                                    gcgTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGAt                                >  4:719091/1‑149 (MQ=255)
                                                                                                                    gcgTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGAt                                <  4:1427991/149‑1 (MQ=255)
                                                                                                                     cgTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATa                               <  2:1420518/149‑1 (MQ=255)
                                                                                                                      gTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAGCCGCGATTTCaa                                                                                    <  4:1163222/95‑1 (MQ=255)
                                                                                                                         gTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATaccac                           >  1:1407596/1‑149 (MQ=255)
                                                                                                                          ttGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGa                                                                >  2:449886/1‑111 (MQ=255)
                                                                                                                            gtgCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCt                                                                           >  4:340955/1‑98 (MQ=255)
                                                                                                                             tgCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTaa                                                                                                >  2:1174557/1‑76 (MQ=255)
                                                                                                                              gCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGAtg                      >  3:275725/1‑149 (MQ=255)
                                                                                                                              gCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGAtg                      >  2:954149/1‑149 (MQ=255)
                                                                                                                                tCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGAtgtg                    >  4:545112/1‑149 (MQ=255)
                                                                                                                                  gTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGc                  <  1:954149/149‑1 (MQ=255)
                                                                                                                                       ttccgTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATa                               <  2:509919/128‑1 (MQ=255)
                                                                                                                                       ttccgTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATa                               >  1:509919/4‑131 (MQ=255)
                                                                                                                                       ccGCGTAAACTTTCGGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGgtg             >  4:329849/1‑149 (MQ=255)
                                                                                                                                       ccGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAat                                                           >  3:336257/1‑103 (MQ=255)
                                                                                                                                       ccGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGc                                         >  1:1240513/1‑121 (MQ=255)
                                                                                                                                        cgcgTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGtt                                    >  3:434697/1‑125 (MQ=255)
                                                                                                                                          cgTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTgtgg          <  2:1266258/149‑1 (MQ=255)
                                                                                                                                          cgTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGa                                                                >  3:356927/1‑95 (MQ=255)
                                                                                                                                          cgTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGAtg                      >  1:460256/1‑137 (MQ=255)
                                                                                                                                           gTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCaa                                                                                    >  4:61915/1‑74 (MQ=255)
                                                                                                                                           gTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCaa                                                                                    <  3:61915/74‑1 (MQ=255)
                                                                                                                                           gTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAg                                                                        >  3:1037727/1‑86 (MQ=255)
                                                                                                                                           gTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAg                                                                        <  4:1037727/86‑1 (MQ=255)
                                                                                                                                           gTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCt                                                                                                    >  2:239327/1‑58 (MQ=38)
                                                                                                                                           gTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCt                                                                                                    <  1:239327/58‑1 (MQ=38)
                                                                                                                                           gTAAACTGAAAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCt                                                                                                    <  4:949726/58‑1 (MQ=38)
                                                                                                                                             aaaCTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGa       >  3:851361/1‑149 (MQ=255)
                                                                                                                                              aaCTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGAt                                <  2:692846/123‑1 (MQ=255)
                                                                                                                                              aaCTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGAt      <  1:159953/149‑1 (MQ=255)
                                                                                                                                               aCTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATa     >  4:1378875/1‑149 (MQ=255)
                                                                                                                                               aCTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATATCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGCTCGCTTGTTTGATACCACCGAGGTGGCCGGTGTGGTGATa     <  1:423774/149‑1 (MQ=255)
                                                                                                                                                cTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAg    <  2:744041/149‑1 (MQ=255)
                                                                                                                                                 tttCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGAt                                >  3:959652/1‑120 (MQ=255)
                                                                                                                                                  ttCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTa  <  1:1130701/149‑1 (MQ=255)
                                                                                                                                                  ttCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGGGATAGTa  >  1:440711/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTA  >  NC_000913/3378138‑3378432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: