Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 288,488 C→G 10.4% V225V (GTG→GTC)  yagI ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913288,4880CG10.4% 154.9 / 8.7 48V225V (GTG→GTC) yagICP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/20);  new base G (3/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGC  >  NC_000913/288351‑288625
                                                                                                                                         |                                                                                                                                         
cgACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                                                                                                     >  2:275845/1‑144 (MQ=255)
cgACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                                                                                                     <  1:275845/144‑1 (MQ=255)
        gctACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                                                                                                     <  1:1108904/136‑1 (MQ=255)
        gctACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                                                                                                     >  2:1108904/1‑136 (MQ=255)
                        ctactTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAcgc                                                                                                                                       >  2:439927/1‑118 (MQ=255)
                        ctactTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAcgc                                                                                                                                       <  1:439927/118‑1 (MQ=255)
                           ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGa                                                                                                                                   <  3:1213682/119‑1 (MQ=255)
                           ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGa                                                                                                                                   >  4:1213682/1‑119 (MQ=255)
                           ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGc                                                                                                                            <  3:1313976/126‑1 (MQ=255)
                           ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGc                                                                                                                            >  4:1313976/1‑126 (MQ=255)
                                                         gCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGgcggcg                                                                                                       >  2:586377/1‑117 (MQ=255)
                                                         gCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGgcggcg                                                                                                       <  1:586377/117‑1 (MQ=255)
                                                         gCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCAc                                                                       >  3:1463039/1‑149 (MQ=255)
                                                              gTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGa                                                                                                   >  2:221232/1‑116 (MQ=255)
                                                              gTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGa                                                                                                   <  1:221232/116‑1 (MQ=255)
                                                                   ccAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGa                                                                                                      <  3:874913/108‑1 (MQ=255)
                                                                   ccAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGa                                                                                                      >  4:874913/1‑108 (MQ=255)
                                                                            gctctcgcgcgcgcCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGcc                                                     <  2:1437747/142‑1 (MQ=255)
                                                                            gctctcgcgcgcgcCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGcc                                                     >  1:1437747/7‑148 (MQ=255)
                                                                            gcgAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGccc                                                    <  4:1463039/149‑1 (MQ=255)
                                                                                    gcgAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGtt                                            >  3:936026/1‑149 (MQ=255)
                                                                                         cccGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGAcgcg                                                                                               >  4:1266552/1‑93 (MQ=255)
                                                                                         cccGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGAcgcg                                                                                               <  3:1266552/93‑1 (MQ=255)
                                                                                         cccGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGGGCAc                                                        >  1:538986/1‑132 (MQ=255)
                                                                                         cccGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGGGCAc                                                        <  2:538986/132‑1 (MQ=255)
                                                                                           cGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCg                                     <  4:936026/149‑1 (MQ=255)
                                                                                                 gctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTc                                                                                  >  2:642831/1‑98 (MQ=255)
                                                                                                 gctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTc                                                                                  <  1:642831/98‑1 (MQ=255)
                                                                                                      aGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGc                          >  3:591532/1‑149 (MQ=255)
                                                                                                              gcgGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCtt                  >  4:156969/1‑149 (MQ=255)
                                                                                                                   aaTTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCgg                                                                                              >  4:151478/1‑68 (MQ=255)
                                                                                                                   aaTTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCgg                                                                                              <  3:151478/68‑1 (MQ=255)
                                                                                                                         ccAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTc       >  3:1148659/1‑149 (MQ=255)
                                                                                                                         ccAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTc       >  1:1447228/1‑149 (MQ=255)
                                                                                                                         ccAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTc       >  1:386183/1‑149 (MQ=255)
                                                                                                                            gCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTgc    <  2:386183/149‑1 (MQ=255)
                                                                                                                            gCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTgc    <  4:591532/149‑1 (MQ=255)
                                                                                                                            ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGcc                                                     <  2:1251625/97‑1 (MQ=255)
                                                                                                                            ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGcc                                                     >  1:1251625/4‑100 (MQ=255)
                                                                                                                            ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAgg                            >  3:405572/4‑125 (MQ=255)
                                                                                                                            ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGACACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAgg                            <  4:405572/122‑1 (MQ=255)
                                                                                                                             tcccGCGTGGACGACGC‑GGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCTCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTgcgc  >  3:1359903/3‑149 (MQ=255)
                                                                                                                             cTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTc                                                                                  >  1:500542/1‑70 (MQ=255)
                                                                                                                             cTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTc                                                                                  <  2:500542/70‑1 (MQ=255)
                                                                                                                              tCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCt      >  1:508844/1‑145 (MQ=255)
                                                                                                                              tCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCt      <  2:508844/145‑1 (MQ=255)
                                                                                                                                      gtcCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTg                                                  <  2:1430960/93‑1 (MQ=255)
                                                                                                                                      gtcCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTg                                                  >  1:1430960/1‑93 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGC  >  NC_000913/288351‑288625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑