Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,401,582 A→T 5.2% L584Q (CTG→CAG)  csrD ← targeting factor for csrBC sRNA degradation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,401,5820AT 5.2% 711.9 / 20.6 193L584Q (CTG→CAG) csrDtargeting factor for csrBC sRNA degradation
Reads supporting (aligned to +/- strand):  ref base A (94/89);  new base T (5/5);  total (99/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 211 total aligned reads displayed.

AAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAA  >  NC_000913/3401434‑3401730
                                                                                                                                                    |                                                                                                                                                    
 aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagcag                                                                                                                                                     <  1:408605/149‑1 (MQ=255)
   tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCagcagc                                                                                                                                                    >  3:1427226/1‑148 (MQ=255)
   tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCagcagc                                                                                                                                                    <  4:1427226/148‑1 (MQ=255)
   tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCt                                                                                                                                                   >  2:195644/1‑149 (MQ=255)
          gAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCTGCTGGTtcaa                                                                                                                                            <  4:1179567/149‑3 (MQ=255)
          gAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtctc                                                                                                                                            >  1:324550/1‑149 (MQ=255)
                gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtctc                                                                                                                                            <  2:486063/143‑1 (MQ=255)
                gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtctc                                                                                                                                            >  1:486063/1‑143 (MQ=255)
                gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCg                                                                                                                                      >  1:1239644/1‑149 (MQ=255)
                  gAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCt                                                                                                                                    >  4:579665/1‑149 (MQ=255)
                    aaaaaTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCTGCTGGTtcaaa                                                                                                                                           >  2:623838/1‑137 (MQ=255)
                           cccTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCaa                                                                                                                              >  4:825179/1‑146 (MQ=255)
                           cccTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCaa                                                                                                                              <  3:825179/146‑1 (MQ=255)
                             cTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGttt                                                                                                                         <  3:1211914/149‑1 (MQ=255)
                                  ccGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTtct                                                                                                                                 >  4:1249445/1‑136 (MQ=255)
                                  ccGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGAc                                                                                                                    <  2:1354881/149‑1 (MQ=255)
                                      cTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTgg                                                                                                                                                 >  3:239877/1‑116 (MQ=255)
                                      cTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAAt                                                                                                                             <  3:11072/136‑1 (MQ=255)
                                      cTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAAt                                                                                                                             >  4:11072/1‑136 (MQ=255)
                                        gTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAAt                                                                                                                             <  2:577257/134‑1 (MQ=255)
                                            cacCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGa                                                                                                          <  3:495333/149‑1 (MQ=255)
                                                gcgcTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGa                                                                                                      >  3:93394/1‑149 (MQ=255)
                                                      aaTCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGa                                                                                                <  2:862053/149‑1 (MQ=255)
                                                        tCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGt                                                                                              >  3:1324107/1‑149 (MQ=255)
                                                        tCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGt                                                                                              >  2:1261133/1‑149 (MQ=255)
                                                          aGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTaa                                                                                            <  3:988176/149‑1 (MQ=255)
                                                           gggTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAAc                                                                                           <  1:1031537/149‑1 (MQ=255)
                                                                  ccACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGt                                                                                                                           <  1:1021077/110‑1 (MQ=255)
                                                                  ccACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAAc                                                                                           <  4:167548/142‑1 (MQ=255)
                                                                  ccACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACAt                                                                                    >  1:1270058/1‑149 (MQ=255)
                                                                    aCTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGAc                                                                                                                    <  2:788853/115‑1 (MQ=255)
                                                                      tCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAg                                                                                <  4:1324107/149‑1 (MQ=255)
                                                                        gCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACt                                                                                          <  3:375283/137‑1 (MQ=255)
                                                                        gCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACt                                                                                          <  2:849177/137‑1 (MQ=255)
                                                                        gCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACt                                                                                          >  4:375283/1‑137 (MQ=255)
                                                                         cTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTc                                                                             <  3:579665/149‑1 (MQ=255)
                                                                          ttCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGa                                                                                                >  2:960651/1‑129 (MQ=255)
                                                                          ttCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGa                                                                                                <  1:960651/129‑1 (MQ=255)
                                                                           tCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCtt                                                                           <  2:324550/149‑1 (MQ=255)
                                                                             gcgAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAg                                                                                               >  1:1191990/1‑127 (MQ=255)
                                                                             gcgAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTg                                                                         >  1:476540/1‑149 (MQ=255)
                                                                               gAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATg                                                                                                        <  4:304765/116‑1 (MQ=255)
                                                                               gAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATg                                                                                                        <  1:859485/116‑1 (MQ=255)
                                                                               gAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATg                                                                                                        >  3:304765/1‑116 (MQ=255)
                                                                               gAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGAt                                                                       <  4:934470/149‑1 (MQ=255)
                                                                                aaCGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTgg                                                                                                                                                 <  3:1028676/74‑1 (MQ=255)
                                                                                aaCGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTgg                                                                                                                                                 >  4:1028676/1‑74 (MQ=255)
                                                                                aaCGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAAt                                                                                                                             >  4:237425/1‑94 (MQ=255)
                                                                                 acgcacgcCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATcc                                                                     <  1:195644/149‑1 (MQ=255)
                                                                                    cacgcCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAAc                                                                  <  2:476540/149‑1 (MQ=255)
                                                                                         caccggCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTa                                                             <  4:346645/145‑1 (MQ=255)
                                                                                           gTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAg                                                                                               >  1:830921/1‑113 (MQ=255)
                                                                                                  aaaCCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTg                                                                <  1:329708/137‑1 (MQ=255)
                                                                                                  aaaCCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTa                                                             >  4:665421/1‑140 (MQ=255)
                                                                                                  aaaCCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTa                                                             <  3:665421/140‑1 (MQ=255)
                                                                                                      cTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTTAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTa                                                             <  4:1067043/136‑1 (MQ=255)
                                                                                                      cTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTaa                                                                                 <  2:1028205/116‑1 (MQ=255)
                                                                                                      cTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTa                                                             >  3:1067043/1‑136 (MQ=255)
                                                                                                      cTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCa                                                >  1:1100759/1‑149 (MQ=255)
                                                                                                              tGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGcc                                        >  1:588998/1‑149 (MQ=255)
                                                                                                               ggTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGa                                                                                                      >  2:341035/1‑86 (MQ=255)
                                                                                                               ggTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTc                                                                                         <  2:906964/99‑1 (MQ=255)
                                                                                                                  cccGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCtt                                                                           <  1:77989/110‑1 (MQ=255)
                                                                                                                   ccGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAg                                                                                >  1:1406680/1‑104 (MQ=255)
                                                                                                                   ccGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAg                                                                                <  2:1406680/104‑1 (MQ=255)
                                                                                                                          aGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAg                                                                                                     >  1:1324110/1‑76 (MQ=255)
                                                                                                                          aGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCtt                                                                           >  2:708880/1‑102 (MQ=255)
                                                                                                                           ggCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCaaa                                              <  3:566890/130‑1 (MQ=255)
                                                                                                                           ggCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCaaa                                              >  4:566890/1‑130 (MQ=255)
                                                                                                                            gCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGCCGGCTAc                          >  3:1258781/1‑149 (MQ=255)
                                                                                                                            gCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTAc                          >  1:281347/1‑149 (MQ=255)
                                                                                                                              ttCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTAc                                                            >  2:1015907/1‑113 (MQ=255)
                                                                                                                              ttCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTAc                                                            <  1:1015907/113‑1 (MQ=255)
                                                                                                                              ttCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTAccc                        >  1:1365058/1‑149 (MQ=255)
                                                                                                                                  accaGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACa                                                                                     >  2:385550/1‑84 (MQ=255)
                                                                                                                                  accaGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAAc                                             >  2:1210285/1‑124 (MQ=255)
                                                                                                                                  accaGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTAc                    >  1:384521/1‑149 (MQ=255)
                                                                                                                                   ccaGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCtt                                                                                                  <  1:664012/70‑1 (MQ=255)
                                                                                                                                    caGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGcc                                        >  3:432949/1‑127 (MQ=255)
                                                                                                                                    caGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGcc                                        <  4:432949/127‑1 (MQ=255)
                                                                                                                                     aGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTAccc                        >  2:495911/1‑142 (MQ=255)
                                                                                                                                     aGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTAccc                        <  1:495911/142‑1 (MQ=255)
                                                                                                                                     aGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTAcccc                 >  3:122732/1‑149 (MQ=255)
                                                                                                                                      ggCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCa                                           <  4:832651/122‑1 (MQ=255)
                                                                                                                                      ggCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCa                                           >  3:832651/1‑122 (MQ=255)
                                                                                                                                       gCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTa               >  3:1007870/1‑149 (MQ=255)
                                                                                                                                         tttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGc                                         <  3:1052083/121‑1 (MQ=255)
                                                                                                                                         tttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGc                                         >  4:1052083/1‑121 (MQ=255)
                                                                                                                                         tttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGtt                                   >  3:226681/1‑127 (MQ=255)
                                                                                                                                         tttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTaaa             >  4:1286325/1‑149 (MQ=255)
                                                                                                                                          ttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGc                                         >  3:1468073/1‑120 (MQ=255)
                                                                                                                                          ttGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGc                                         <  4:1468073/120‑1 (MQ=255)
                                                                                                                                            gAACCAGCTGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTaaa             <  2:1315044/146‑1 (MQ=255)
                                                                                                                                             aaCCAGCTGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAt         >  4:911099/1‑149 (MQ=255)
                                                                                                                                             aaCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAt         >  1:895783/1‑149 (MQ=255)
                                                                                                                                              aCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAtt        >  4:512261/1‑149 (MQ=255)
                                                                                                                                               ccAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAtt        >  3:751052/1‑148 (MQ=255)
                                                                                                                                               ccAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCAtt        <  4:751052/148‑1 (MQ=255)
                                                                                                                                                  gcagcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACt    >  2:1088761/1‑149 (MQ=255)
                                                                                                                                                    agcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTaa  >  2:275477/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAA  >  NC_000913/3401434‑3401730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: