Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,522,778 C→G 5.4% intergenic (‑27/‑93) hofM ← / → mrcA DNA catabolic putative pilus assembly protein/penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,522,7780CG 5.4% 467.6 / 13.6 129intergenic (‑27/‑93)hofM/mrcADNA catabolic putative pilus assembly protein/penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (61/61);  new base G (5/2);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 131 total aligned reads displayed.

TTCTCCAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGG  >  NC_000913/3522630‑3522922
                                                                                                                                                    |                                                                                                                                                
ttCTCCAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAc                                                                                                                                                  >  3:427275/1‑149 (MQ=255)
  ctcCAGCGGCAACCGCCACCAGCGTTGCACAAAGCATTCTTTTGCGCCCCGCATGATCGCAAACGCTACCGATTCTTGCTGTTGTAACTGCAACCCAATTTGCCAGATCTTACATGCCATTGTGATGATCTCCTTCTCAACCGTCCctc                                                                                                                                                <  4:902129/149‑1 (MQ=255)
       gCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGAc                                                                                                                                            >  4:12105/1‑148 (MQ=255)
          gCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGt                                                                                                                                        <  4:427275/149‑1 (MQ=255)
            aaCCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGAc                                                                                                                                            >  2:905740/1‑143 (MQ=255)
            aaCCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGAc                                                                                                                                            <  1:905740/143‑1 (MQ=255)
            aaCCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACgg                                                                                                                                          >  4:535509/1‑145 (MQ=255)
            aaCCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACgg                                                                                                                                          <  3:535509/145‑1 (MQ=255)
             aCCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGtata                                                                                                                                     <  3:1161142/149‑1 (MQ=255)
                     caGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCg                                                                                                                             >  1:1398260/1‑149 (MQ=255)
                             gcaaaaGAATGCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCtt                                                                                                                     >  3:1453333/3‑149 (MQ=255)
                              aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGc                                                                                                                              <  1:1201422/139‑1 (MQ=255)
                               aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACggg                                                                                                                                         <  2:684072/127‑1 (MQ=255)
                               aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACggg                                                                                                                                         >  1:684072/1‑127 (MQ=255)
                                aaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCaa                                                                                                                                 >  1:1118535/1‑134 (MQ=255)
                                aaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCaa                                                                                                                                 <  2:1118535/134‑1 (MQ=255)
                                  aGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGc                                                                                                                              <  1:1330570/135‑1 (MQ=255)
                                  aGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCtt                                                                                                                <  1:196760/149‑1 (MQ=255)
                                     aTTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtg                                                                                                                                       >  4:298181/1‑122 (MQ=255)
                                      ttCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtata                                                                                                            >  3:677117/1‑149 (MQ=255)
                                         ttttGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCTTTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCaa                                                                                                                                 <  2:500874/125‑1 (MQ=255)
                                         ttttGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCaa                                                                                                                                 >  1:500874/1‑125 (MQ=255)
                                               gcCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                             >  1:646092/1‑139 (MQ=255)
                                               gcCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGCATATCAATGCGTCTGGCTTGCCTTtat                                                                                                             <  2:646092/139‑1 (MQ=255)
                                                 cccGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgt                                                                                                 >  2:1228308/1‑149 (MQ=255)
                                                   cgaaCGATCGCAACCGCTACCGCTTCTTGCTGTTATAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACggg                                                                                                                                         >  3:971875/4‑107 (MQ=255)
                                                    gCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACgg                                                                                                                                          <  4:245188/105‑1 (MQ=255)
                                                     cACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgt                                                                                             <  4:677117/149‑1 (MQ=255)
                                                      aCGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgtt                                                                                            <  2:1398260/149‑1 (MQ=255)
                                                        gATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                             >  1:1263613/1‑130 (MQ=255)
                                                        gATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                             <  2:1263613/130‑1 (MQ=255)
                                                         aTCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGc                                                                                                                              >  2:1273450/1‑112 (MQ=255)
                                                         aTCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTtat                                                                                         <  4:1453333/149‑1 (MQ=255)
                                                              aaCCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATaaa                                                                                      <  3:493856/147‑1 (MQ=255)
                                                              aaCCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACt                                                                                    >  4:118512/1‑149 (MQ=255)
                                                                 cgctaccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGcc                                                                                 >  1:1000268/1‑149 (MQ=255)
                                                                    taccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGcc                                                                                 <  3:244437/146‑1 (MQ=255)
                                                                    taccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGcc                                                                                 >  4:244437/1‑146 (MQ=255)
                                                                       cgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATg                                                                           >  4:921138/1‑149 (MQ=255)
                                                                          ttcttGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaaa                                                                        <  3:118512/149‑1 (MQ=255)
                                                                          ttcttGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaaa                                                                        <  1:1228308/149‑1 (MQ=255)
                                                                               gCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTaaa                                                                   <  3:921138/149‑1 (MQ=255)
                                                                                   ttgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGc                                                                                                                   <  3:1210916/97‑1 (MQ=255)
                                                                                   ttgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTaaa                                                                   >  3:667465/1‑145 (MQ=255)
                                                                                   ttgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTACAAggg                                                               >  4:944033/1‑149 (MQ=255)
                                                                                   ttgtAAATGCAAACCAATTTGCCAGATCGTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTaaa                                                                   <  4:667465/145‑1 (MQ=255)
                                                                                      tAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTATAAACTGCCCAAATGaaa                                                                        >  2:822723/1‑136 (MQ=255)
                                                                                       aaaTGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGc                                                                                                                              >  3:1385900/1‑82 (MQ=255)
                                                                                        aaTGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAAtt                                                          <  3:944033/149‑1 (MQ=255)
                                                                                        aaTGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAAtt                                                          >  3:1174393/1‑149 (MQ=255)
                                                                                         aTGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAAttt                                                         >  1:342928/1‑149 (MQ=255)
                                                                                          tGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTc                                                        <  4:489692/149‑1 (MQ=255)
                                                                                                ccAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgtt                                                                                            <  2:1105411/107‑1 (MQ=255)
                                                                                                ccAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgtt                                                                                            >  1:1105411/1‑107 (MQ=255)
                                                                                                 cAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGAc                                                                                                                                            >  2:1120840/1‑58 (MQ=255)
                                                                                                  aaTTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGtat                                                                                                                                      >  2:1036594/1‑63 (MQ=255)
                                                                                                  aaTTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGtat                                                                                                                                      <  1:1036594/63‑1 (MQ=255)
                                                                                                        ccAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgt                                                                                             >  3:355191/1‑98 (MQ=255)
                                                                                                        ccAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAAc                                                                                     <  1:896785/106‑1 (MQ=255)
                                                                                                        ccAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAAc                                                                                     >  2:896785/1‑106 (MQ=255)
                                                                                                          aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                        <  1:1387990/149‑1 (MQ=255)
                                                                                                          aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                        >  2:1387990/1‑149 (MQ=255)
                                                                                                          aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                        <  1:29558/149‑1 (MQ=255)
                                                                                                           gATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCt                                                                                                                 <  3:1295514/75‑1 (MQ=255)
                                                                                                           gATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCt                                                                                                                 >  4:1295514/1‑75 (MQ=255)
                                                                                                            aTCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAAt                                                                  <  3:1241270/121‑1 (MQ=255)
                                                                                                            aTCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAAt                                                                  >  4:1241270/1‑121 (MQ=255)
                                                                                                             tCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtata                                                                                                            <  4:543968/78‑1 (MQ=255)
                                                                                                             tCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaaa                                                                        >  1:1385781/1‑114 (MQ=255)
                                                                                                             tCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaaa                                                                        <  2:1385781/114‑1 (MQ=255)
                                                                                                                tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttt                                  <  1:204575/149‑1 (MQ=255)
                                                                                                                tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttt                                  <  2:342928/149‑1 (MQ=255)
                                                                                                                 aaaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttt                                 <  3:554429/149‑1 (MQ=255)
                                                                                                                  aaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttt                                 <  1:1149927/148‑1 (MQ=255)
                                                                                                                  aaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttt                                 >  2:1149927/1‑148 (MQ=255)
                                                                                                                    tGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTAtacta                                                                                                         <  1:858154/74‑1 (MQ=255)
                                                                                                                       cATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgtt                                                                                            >  3:617513/1‑84 (MQ=255)
                                                                                                                       cATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgtt                                                                                            <  4:617513/84‑1 (MQ=255)
                                                                                                                             gatgatCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATcc                          <  2:1151220/144‑1 (MQ=255)
                                                                                                                             gatgatCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATcc                          >  1:1151220/1‑144 (MQ=255)
                                                                                                                              atgatCTCCTTATCACCCGTCACTCTGACGGGTATATTAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCa                                                      <  2:1045839/115‑1 (MQ=255)
                                                                                                                              atgatCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCa                                                      >  1:1045839/1‑115 (MQ=255)
                                                                                                                                 atCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCt                 <  4:1377290/149‑1 (MQ=255)
                                                                                                                                    tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTcc                                                       >  4:11149/1‑108 (MQ=255)
                                                                                                                                    tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTcc                                                       <  3:11149/108‑1 (MQ=255)
                                                                                                                                     ccTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttttt                                >  3:536138/1‑130 (MQ=255)
                                                                                                                                     ccTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttttt                                <  4:536138/130‑1 (MQ=255)
                                                                                                                                      cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTc                                                        <  1:1132893/105‑1 (MQ=255)
                                                                                                                                      cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt              <  1:930792/147‑1 (MQ=255)
                                                                                                                                      cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt              >  2:930792/1‑147 (MQ=255)
                                                                                                                                       ttATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgt                                                                                             >  1:103312/1‑67 (MQ=255)
                                                                                                                                       ttATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgt                                                                                             <  2:103312/67‑1 (MQ=255)
                                                                                                                                         aTCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCAtt         >  2:1137029/1‑149 (MQ=255)
                                                                                                                                          tCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTc        >  1:736417/1‑149 (MQ=255)
                                                                                                                                            aCCCGTCAGAGTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGAt                            <  2:180456/127‑1 (MQ=255)
                                                                                                                                            aCCCGTCAGAGTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGAt                            >  1:180456/1‑127 (MQ=255)
                                                                                                                                               cGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGa                                                              >  4:454215/1‑90 (MQ=255)
                                                                                                                                               cGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGa                                                              <  3:454215/90‑1 (MQ=255)
                                                                                                                                                gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTgg  <  1:1095227/149‑1 (MQ=255)
                                                                                                                                                gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTgg  <  2:572124/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTCTCCAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGG  >  NC_000913/3522630‑3522922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: