Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,533,039 T→G 6.1% R74R (CGT→CGG) ‡ pck → phosphoenolpyruvate carboxykinase [ATP]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,533,0390TG 6.1% 581.4 / 18.0 160R74R (CGT→CGG) ‡pckphosphoenolpyruvate carboxykinase [ATP]
Reads supporting (aligned to +/- strand):  ref base T (71/79);  new base G (2/8);  total (73/87)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

ACCCAAGCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTT  >  NC_000913/3532894‑3533177
                                                                                                                                                 |                                                                                                                                          
  ccAAGCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGa                                                                                                                                       <  3:385832/149‑1 (MQ=255)
   cAAGCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCACGGACGAt                                                                                                                                      >  2:353559/1‑149 (MQ=255)
    aaGCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATa                                                                                                                                     <  1:665781/149‑1 (MQ=255)
    aaGCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATa                                                                                                                                     <  3:285227/149‑1 (MQ=255)
    aaGCTACGACCAGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCACGGACGATa                                                                                                                                     <  1:357604/149‑1 (MQ=255)
      gCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCACGGACGATata                                                                                                                                   <  2:1109879/149‑3 (MQ=255)
      gCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTACGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCACGGACGATata                                                                                                                                   <  2:1279120/149‑3 (MQ=255)
       cTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCACGGACGATatac                                                                                                                                  <  2:1405662/149‑4 (MQ=255)
         aCGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGt                                                                                                                                            <  3:527386/137‑1 (MQ=255)
             ccTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGAGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCACGGACGATata                                                                                                                                   <  1:32773/142‑3 (MQ=255)
               tgctgTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGAt                                                                                                                          <  2:106197/149‑1 (MQ=255)
                gctgTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATa                                                                                                                         >  3:616204/1‑149 (MQ=255)
                 ctgTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGACGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATAc                                                                                                                        <  4:1199722/149‑1 (MQ=255)
                    tATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACttt                                                                                                                     >  4:287226/1‑149 (MQ=255)
                     aTCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTc                                                                                                                    <  4:1418900/149‑1 (MQ=255)
                      tCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCt                                                                                                                   >  1:65800/1‑149 (MQ=255)
                       cAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtg                                                                                                                  <  1:992941/149‑1 (MQ=255)
                         ggAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCt                                                                                                                   <  1:1009786/146‑1 (MQ=255)
                         ggAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCt                                                                                                                   >  2:1009786/1‑146 (MQ=255)
                          gAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATAc                                                                                                                        <  2:439979/140‑1 (MQ=255)
                          gAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtggtg                                                                                                               >  3:617295/1‑149 (MQ=255)
                          gAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtggt                                                                                                                >  2:1462663/1‑148 (MQ=255)
                          gAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtggt                                                                                                                <  1:1462663/148‑1 (MQ=255)
                           aaGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtggtgg                                                                                                              <  4:1048788/149‑1 (MQ=255)
                             gagCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGc                                                                                                            <  4:448459/149‑1 (MQ=255)
                              agCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCa                                                                                                           <  4:1204041/149‑1 (MQ=255)
                              agCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCa                                                                                                           <  1:1433287/149‑1 (MQ=255)
                               gCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGt                                                                                                                                            >  3:425036/1‑115 (MQ=255)
                                   gATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTc                                                                                                                    <  2:1366551/135‑1 (MQ=255)
                                   gATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTc                                                                                                                    >  1:1366551/1‑135 (MQ=255)
                                     tCCGGGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    <  2:65800/149‑1 (MQ=255)
                                     tCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    <  4:617295/149‑1 (MQ=255)
                                     tCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    <  3:640061/149‑1 (MQ=255)
                                       cGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggc                                                                                                  >  3:206109/1‑149 (MQ=255)
                                           ccTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              <  1:932185/149‑1 (MQ=255)
                                           ccTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              <  2:85406/149‑1 (MQ=255)
                                               aCAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCAGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTaa                                                                                          >  1:114826/1‑149 (MQ=255)
                                                    ttATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGTTGGGCAGACAAAGGCAAAGGTAAGAACg                                                                                     >  3:1167667/1‑149 (MQ=255)
                                                     tATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGa                                                                                    <  3:287226/149‑1 (MQ=255)
                                                     tATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGa                                                                                    <  3:978599/149‑1 (MQ=255)
                                                                  tgtTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              <  3:1464557/126‑1 (MQ=255)
                                                                  tgtTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    >  1:439619/1‑120 (MQ=255)
                                                                    ttAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaa                                                                                                     >  1:510152/1‑117 (MQ=255)
                                                                     tAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTc                                                                                                                    <  4:131775/101‑1 (MQ=255)
                                                                      aaCTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              >  1:481950/1‑122 (MQ=255)
                                                                      aaCTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              <  2:481950/122‑1 (MQ=255)
                                                                      aaCTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCtctctctc                                                                   >  4:370535/1‑149 (MQ=255)
                                                                       aCTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATAc                                                                                                                        <  2:1445177/95‑1 (MQ=255)
                                                                       aCTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTcc                                                                  <  1:187341/149‑1 (MQ=255)
                                                                       aCTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACAAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTcc                                                                  >  4:655640/1‑149 (MQ=255)
                                                                       aCTAATCTGGGTGCCGTTGCCGTCGATAACGAGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACACGCGATACGTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTcc                                                                  >  4:900181/1‑149 (MQ=255)
                                                                         tAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    >  1:262077/1‑113 (MQ=255)
                                                                         tAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    <  2:262077/113‑1 (MQ=255)
                                                                          aaTCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGaa                                                                                       >  1:1324602/1‑125 (MQ=255)
                                                                          aaTCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGaa                                                                                       <  2:1324602/125‑1 (MQ=255)
                                                                          aaTCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGacaa                                                                                 >  3:1223477/1‑131 (MQ=255)
                                                                          aaTCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACaaa                                                                             >  2:513549/1‑135 (MQ=255)
                                                                           aTCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGaa                                                              >  3:856962/1‑149 (MQ=255)
                                                                           aTCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGaa                                                              >  2:1195633/1‑149 (MQ=255)
                                                                                 gTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTgg                                                        <  2:975501/149‑1 (MQ=255)
                                                                                 gTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTgg                                                        <  3:655640/149‑1 (MQ=255)
                                                                                      gttgccgtCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGAGAGGTATATCGTCCATGACGATACCACTCGCGATACTTTCTGGCGGGCAGACAATGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCTGAAACATGgcaaca                                                   >  4:902895/1‑149 (MQ=255)
                                                                                       ttgccgtCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtggt                                                                                                                >  3:1023513/1‑87 (MQ=255)
                                                                                       ttgccgtCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAAc                                                                            <  4:175482/123‑1 (MQ=255)
                                                                                        tgccgtCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGAt                                                                                                                                      <  1:971267/64‑1 (MQ=255)
                                                                                        tgccgtCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGAt                                                                                                                                      >  2:971267/1‑64 (MQ=255)
                                                                                            gtCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGaa                                             >  4:813858/1‑149 (MQ=255)
                                                                                             tCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATACGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGACAGACAAAGGCAAAGGTAAGAACGACAAAAAACGTCTCTCTCCGGAAACCTGGCTGCATCTGaaa                                            <  3:902895/149‑1 (MQ=255)
                                                                                               gATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAgg                                          <  4:856962/149‑1 (MQ=255)
                                                                                                 tACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGa                                                                                    >  1:870685/1‑105 (MQ=255)
                                                                                                 tACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAAc                                                                            >  4:466670/1‑113 (MQ=255)
                                                                                                      ggATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCtg                                                                                                                  <  3:135249/70‑1 (MQ=255)
                                                                                                      ggATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTggg                                                                                                             <  4:283521/75‑1 (MQ=255)
                                                                                                      ggATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGa                                                                                    <  1:1405598/100‑1 (MQ=255)
                                                                                                         tCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGcc                                        <  4:1433514/141‑1 (MQ=255)
                                                                                                         tCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGcc                                        >  3:1433514/1‑141 (MQ=255)
                                                                                                                       tCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCgg                  <  2:164793/149‑1 (MQ=255)
                                                                                                                           cAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTg                                      >  3:897587/1‑125 (MQ=255)
                                                                                                                           cAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGt                                    <  4:621635/127‑1 (MQ=255)
                                                                                                                           cAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGAcc                                >  2:571927/1‑131 (MQ=255)
                                                                                                                           cAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGAcc                                <  1:571927/131‑1 (MQ=255)
                                                                                                                           cAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTcc                    <  2:1083365/143‑1 (MQ=255)
                                                                                                                             aaaGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTaa                                                                                          >  1:1446544/1‑71 (MQ=255)
                                                                                                                             aaaGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGc                            >  1:69660/1‑133 (MQ=255)
                                                                                                                             aaaGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGc                            <  2:69660/133‑1 (MQ=255)
                                                                                                                                gATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCt         >  3:353631/1‑149 (MQ=255)
                                                                                                                                  tAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCt         >  1:22720/1‑147 (MQ=255)
                                                                                                                                  tAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCt         <  2:22720/147‑1 (MQ=255)
                                                                                                                                  tAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGt       <  3:813858/149‑1 (MQ=255)
                                                                                                                                   aaGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGtt      >  2:203688/1‑149 (MQ=255)
                                                                                                                                     gTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              <  1:491362/59‑1 (MQ=255)
                                                                                                                                     gTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaaggcaaag                                                                                              >  2:491362/1‑59 (MQ=255)
                                                                                                                                     gTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTg                                      >  3:681794/1‑115 (MQ=255)
                                                                                                                                     gTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCa                           >  4:966861/1‑126 (MQ=255)
                                                                                                                                     gTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCa                           <  3:966861/126‑1 (MQ=255)
                                                                                                                                       gtatCGTCACGGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCg                   <  4:684483/131‑1 (MQ=255)
                                                                                                                                       atatCGTCCGTGACGATACCACTCGCGATACTTTGTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCCGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGtt  <  1:1195633/149‑1 (MQ=255)
                                                                                                                                       atatCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGtt  <  4:580478/149‑1 (MQ=255)
                                                                                                                                            gTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGAcaaag                                                                                                    <  1:1010458/46‑1 (MQ=255)
                                                                                                                                                 tGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGAc                                 <  2:1417582/108‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
ACCCAAGCTACGACCTGCTGTATCAGGAAGAGCTCGATCCGAGCCTGACAGGTTATGAGCGCGGGGTGTTAACTAATCTGGGTGCCGTTGCCGTCGATACCGGGATCTTCACCGGTCGTTCACCAAAAGATAAGTATATCGTCCGTGACGATACCACTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTT  >  NC_000913/3532894‑3533177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: