| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 294,915 | T→A | 6.8% | intergenic (‑116/+224) | yagM ← / ← yagN | CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 294,915 | 0 | T | A | 6.8% | 208.6 / 4.3 | 59 | intergenic (‑116/+224) | yagM/yagN | CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base T (31/24); new base A (2/2); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGAATGGTTTGAGAT > NC_000913/294776‑295062 | aaaaTTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGa > 1:1367820/1‑149 (MQ=255) aGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTtct < 2:1367820/149‑1 (MQ=255) acTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTTAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACa > 2:606111/1‑149 (MQ=255) tAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGaaaa < 4:115223/149‑1 (MQ=255) tttttAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAAt < 3:1329726/144‑1 (MQ=255) tttttAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAAt > 4:1329726/1‑144 (MQ=255) aaCCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTAt < 1:1298540/138‑1 (MQ=255) aaCCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTAt > 2:1298540/1‑138 (MQ=255) ctctTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTAt > 1:184820/1‑135 (MQ=255) ctctTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTAt < 2:184820/135‑1 (MQ=255) tctTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGa > 1:269446/1‑149 (MQ=255) tATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCttt < 2:269446/149‑1 (MQ=255) tactaTCTTAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATgg < 1:606111/149‑1 (MQ=255) tactaTCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGt > 4:1432506/1‑109 (MQ=255) tactaTCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGt < 3:1432506/109‑1 (MQ=255) aGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCg < 1:491423/79‑1 (MQ=255) aGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCg > 2:491423/1‑79 (MQ=255) cccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTgg < 2:381773/143‑1 (MQ=255) cccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTgg > 1:381773/1‑143 (MQ=255) cccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAgg > 3:619831/1‑149 (MQ=255) ccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATttat < 3:384588/56‑1 (MQ=255) ccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATttat > 4:384588/1‑56 (MQ=255) ccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGtttt < 4:139571/140‑1 (MQ=255) ccATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGtttt > 3:139571/1‑140 (MQ=255) cATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTTTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTa > 2:599963/1‑149 (MQ=255) aTTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACa < 2:384043/72‑1 (MQ=255) aTTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACa > 1:384043/1‑72 (MQ=255) tttttCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAg > 3:220527/1‑148 (MQ=255) tttttCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAg < 4:220527/148‑1 (MQ=255) gggCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAAt < 2:151381/108‑1 (MQ=255) gggCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAAt > 1:151381/1‑108 (MQ=255) aTCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGt > 4:1247097/1‑149 (MQ=255) tCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTa > 2:634905/1‑98 (MQ=255) tCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTa < 1:634905/98‑1 (MQ=255) tCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTc < 3:1247097/149‑1 (MQ=255) tataAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGa < 1:489356/108‑1 (MQ=255) tataAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGa > 2:489356/1‑108 (MQ=255) tCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGc > 2:517032/1‑149 (MQ=255) tAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGc > 3:1092158/1‑118 (MQ=255) tAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGc < 4:1092158/118‑1 (MQ=255) tAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCAtt > 4:1118293/1‑149 (MQ=255) tAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCAtt > 2:1222193/1‑149 (MQ=255) aaaTTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAg < 4:1035997/115‑1 (MQ=255) aaaTTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAg > 3:1035997/1‑115 (MQ=255) aaTTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTAc < 1:517032/149‑1 (MQ=255) aaTTAAATCTGTAAGTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTAc > 2:1018934/1‑149 (MQ=255) ttAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGa > 3:111792/1‑149 (MQ=255) aaaTCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGAAt > 3:1236687/1‑149 (MQ=255) gtCTGTAATAAATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGaa > 3:188685/2‑146 (MQ=255) gtCTGTAATAAATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGaa < 4:188685/145‑1 (MQ=255) tCTGTAATAAATTACAGTCTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAgg < 2:883627/116‑1 (MQ=255) tCTGTAATAAATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAgg > 1:883627/1‑116 (MQ=255) cTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTg > 3:1350425/1‑96 (MQ=255) cTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTg < 4:1350425/96‑1 (MQ=255) tGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGAttt < 1:508199/89‑1 (MQ=255) tGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGAttt > 2:508199/1‑89 (MQ=255) tGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGa > 3:422284/1‑127 (MQ=255) tGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGa < 4:422284/127‑1 (MQ=255) aTTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGa > 2:545271/1‑138 (MQ=255) aTTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGa < 1:545271/138‑1 (MQ=255) aTTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGAATGGTTTgaga > 3:1395118/1‑149 (MQ=255) tttATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGtt > 4:538312/1‑87 (MQ=255) tttATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGtt < 3:538312/87‑1 (MQ=255) tttATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGc > 4:238903/1‑101 (MQ=255) tttATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGc < 3:238903/101‑1 (MQ=255) tttATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGAATGGTTTGAGAt > 3:1446441/1‑149 (MQ=255) | AAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGTAATTTATTACAGACTTTCTCGTACAGAAAATGTATCTATAAAATCGAGGACATCTTTACGTAACCAGTAATGGCGGCCATTGATTTGTTTTGGGCGAGGTAGCCCCTCAGGCAATTTGTCCTGACAAAGCCATTTACGAATGGTTTGAGAT > NC_000913/294776‑295062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |