Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,563,554 G→A 6.3% intergenic (+36/+233) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,563,5540GA 6.3% 418.8 / 12.6 126intergenic (+36/+233)glpD/yzgLsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
Reads supporting (aligned to +/- strand):  ref base G (58/60);  new base A (4/4);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

Only 100 of 128 total aligned reads displayed.

GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAA  >  NC_000913/3563408‑3563688
                                                                                                                                                  |                                                                                                                                      
gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGcc                                                                                                                                      >  2:338568/1‑149 (MQ=255)
  caccgggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTg                                                                                                                                    >  3:1031726/6‑149 (MQ=255)
         cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtga                                                                                                                             <  4:144902/149‑1 (MQ=255)
           tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgatg                                                                                                                           >  3:1320749/1‑149 (MQ=255)
             gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATTGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGAc                                                                                                                         >  3:880121/1‑149 (MQ=255)
               acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaa                                                                                                                       >  1:985024/1‑149 (MQ=255)
               acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTgat                                                                                                                                  <  4:1186323/138‑2 (MQ=255)
               acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTgat                                                                                                                                  >  3:1186323/1‑137 (MQ=255)
                caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaaa                                                                                                                      <  1:338568/149‑1 (MQ=255)
                 aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTgaga                                                                                                                                 >  4:1103545/1‑137 (MQ=255)
                 aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTgaga                                                                                                                                 <  3:1103545/137‑1 (MQ=255)
                 aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGAGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGAACAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAac                                                                                                                     >  1:1140187/1‑149 (MQ=255)
                           aggtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGc                                                                                                           <  2:421219/147‑1 (MQ=255)
                            tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATTGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGcc                                                                                                          <  4:880121/149‑1 (MQ=255)
                                gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGAt                                                                                                      >  1:472101/1‑149 (MQ=255)
                                  tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTATCGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGc                                                                                                           <  3:852103/142‑1 (MQ=255)
                                  tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtga                                                                                                                             >  3:1284848/1‑124 (MQ=255)
                                  tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATgc                                                                                                    <  4:1320749/149‑1 (MQ=255)
                                    aaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTgg                                                                                                                                        <  4:823833/111‑1 (MQ=255)
                                    aaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTgg                                                                                                                                        >  3:823833/1‑111 (MQ=255)
                                      tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACaaaa                                                                                                               >  2:1298097/1‑134 (MQ=255)
                                         ggATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTga                                                                                                                                   <  1:4319/111‑1 (MQ=255)
                                         ggATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTga                                                                                                                                   >  2:4319/1‑111 (MQ=255)
                                          gATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCt                                                                                            >  4:1064438/1‑149 (MQ=255)
                                           aTCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCt                                                                                                                                     <  1:1446466/107‑1 (MQ=255)
                                           aTCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCt                                                                                                                                     >  2:1446466/1‑107 (MQ=255)
                                            tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTgat                                                                                                                                  >  3:994783/1‑108 (MQ=39)
                                             caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTAt                                                                                         <  4:1031726/149‑1 (MQ=255)
                                              aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTg                                                                                                                                    <  1:875157/105‑1 (MQ=255)
                                              aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTg                                                                                                                                    >  2:875157/1‑105 (MQ=255)
                                              aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat                                                                                                                            <  1:296135/113‑1 (MQ=255)
                                              aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATc                                                                                        <  1:110294/149‑1 (MQ=255)
                                              aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATc                                                                                        >  3:1126949/1‑149 (MQ=255)
                                                caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAg                                                                                      <  2:337801/149‑1 (MQ=255)
                                                  aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGc                                                                                    >  3:1461280/1‑149 (MQ=255)
                                                  aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGc                                                                                    >  2:55840/1‑149 (MQ=255)
                                                   tctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGcc                                                                                   >  3:1160656/1‑149 (MQ=255)
                                                        tgtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATc                                                                                        <  3:1019591/139‑1 (MQ=255)
                                                        tgtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGt                                                                              <  4:1461280/149‑1 (MQ=255)
                                                                  tGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAgg                                                                                     >  4:462076/1‑132 (MQ=255)
                                                                   ggCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCaa                                                                   <  1:739935/149‑1 (MQ=255)
                                                                   ggCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCaa                                                                   <  2:1140187/149‑1 (MQ=255)
                                                                     cTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCtt                                                                                           >  1:1336739/1‑123 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACg                                                                                              <  1:335255/112‑1 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACg                                                                                              >  2:335255/1‑112 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCtt                                                                                           >  3:607438/1‑115 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATc                                                                                        <  4:1180906/118‑1 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGtt                                                                          <  3:632019/132‑1 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGtt                                                                          >  4:632019/1‑132 (MQ=255)
                                                                             gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAt                                                         >  4:4763/1‑149 (MQ=255)
                                                                              tataCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGa                                                                                                       >  1:34011/1‑102 (MQ=255)
                                                                                taCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAacac                                                                                                                   <  4:491221/88‑1 (MQ=255)
                                                                                taCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAacac                                                                                                                   >  3:491221/1‑88 (MQ=255)
                                                                                taCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGc                                                                     >  4:390834/1‑134 (MQ=255)
                                                                                taCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGc                                                                     <  3:390834/134‑1 (MQ=255)
                                                                                  cGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAgg                                                                                     >  4:720936/1‑116 (MQ=255)
                                                                                   gcagcaGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaaa                                                                                                                      <  4:551797/82‑1 (MQ=255)
                                                                                   gcagcaGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCaa                                                                   >  2:177981/1‑133 (MQ=255)
                                                                                      gcagAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATAtt                                                             >  2:469899/1‑136 (MQ=255)
                                                                                          agGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                               >  3:59325/1‑114 (MQ=255)
                                                                                          agGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                               <  4:59325/114‑1 (MQ=255)
                                                                                            gTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAttt                                                       >  1:386775/1‑136 (MQ=255)
                                                                                            gTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAttt                                                       <  2:386775/136‑1 (MQ=255)
                                                                                             ttATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAata                                                                 >  2:1335588/1‑125 (MQ=255)
                                                                                             ttATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAata                                                                 <  1:1335588/125‑1 (MQ=255)
                                                                                                   cTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGa                                   <  3:534673/149‑1 (MQ=255)
                                                                                                   cTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGa                                   <  3:4763/149‑1 (MQ=255)
                                                                                                    tGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAatata                                                               >  4:802652/1‑120 (MQ=255)
                                                                                                    tGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAatata                                                               <  3:802652/120‑1 (MQ=255)
                                                                                                        gtcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat                                                                                                                            >  2:714335/1‑55 (MQ=255)
                                                                                                        gtcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGa                              >  4:118704/1‑149 (MQ=255)
                                                                                                         tcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGAc                             <  1:55840/149‑1 (MQ=255)
                                                                                                         tcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGAc                             >  2:891218/1‑149 (MQ=255)
                                                                                                           gtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGa                              >  1:1005432/1‑146 (MQ=255)
                                                                                                           gtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGa                              <  2:1005432/146‑1 (MQ=255)
                                                                                                            tAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGTCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGtt                          <  4:1126949/149‑1 (MQ=255)
                                                                                                            tAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGtt                          >  2:1408824/1‑149 (MQ=255)
                                                                                                              aaTTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTc                         <  1:632667/148‑1 (MQ=255)
                                                                                                              aaTTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTc                         >  2:632667/1‑148 (MQ=255)
                                                                                                               aTTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaaa                                                                                                                      >  4:868534/1‑54 (MQ=255)
                                                                                                                ttACCGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGa                                   <  3:386110/136‑1 (MQ=255)
                                                                                                                ttAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATaa                                >  2:281641/1‑139 (MQ=255)
                                                                                                                ttAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATaa                                <  1:281641/139‑1 (MQ=255)
                                                                                                                      tAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTAt                                                                       <  2:804484/94‑1 (MQ=255)
                                                                                                                      tAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTAt                                                                       >  1:804484/1‑94 (MQ=255)
                                                                                                                        aGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTc                         <  4:550016/138‑1 (MQ=255)
                                                                                                                        aGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTc                         >  3:550016/1‑138 (MQ=255)
                                                                                                                        aGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATcc              <  1:1408824/149‑1 (MQ=255)
                                                                                                                            gATCAGGTCAGATTTCTATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGtt                                                                          <  3:1019514/85‑1 (MQ=255)
                                                                                                                            gATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGtt                                                                          >  4:1019514/1‑85 (MQ=255)
                                                                                                                              tCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATgg                                                 >  2:915063/1‑108 (MQ=255)
                                                                                                                              tCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATgg                                                 <  1:915063/108‑1 (MQ=255)
                                                                                                                              tCAGGCCAGATTGAAATCTGACCTGAGACTGATGACAAAGACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGc           <  1:1189947/146‑1 (MQ=21)
                                                                                                                              tCAGGCCAGATTGAAATCTGACCTGAGACTGATGACAAACACAAAACTGCCTGATGCACTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGc           >  2:1189947/1‑146 (MQ=21)
                                                                                                                                  gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCAt                                                   >  1:724218/1‑102 (MQ=255)
                                                                                                                                   tCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTc                    >  3:1178558/1‑132 (MQ=255)
                                                                                                                                   tCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTc                    <  4:1178558/132‑1 (MQ=255)
                                                                                                                                    cAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACaaa  <  3:118704/149‑1 (MQ=255)
                                                                                                                                       aTTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGTCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGAt                                  <  4:860147/114‑1 (MQ=255)
                                                                                                                                       aTTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGTCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGAt                                  >  3:860147/1‑114 (MQ=255)
                                                                                                                                                  |                                                                                                                                      
GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAA  >  NC_000913/3563408‑3563688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: