Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,630,661 A→T 6.9% intergenic (‑59/+307) yhiI ← / ← yhiJ putative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,630,6610AT 6.9% 616.7 / 25.7 173intergenic (‑59/+307)yhiI/yhiJputative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base A (80/81);  new base T (7/5);  total (87/86)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 181 total aligned reads displayed.

CCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGC  >  NC_000913/3630515‑3630807
                                                                                                                                                  |                                                                                                                                                  
ccGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAacgacg                                                                                                                                                  >  2:53192/1‑149 (MQ=255)
      gcAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGc                                                                                                                                            <  3:364264/149‑1 (MQ=255)
      gcAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGc                                                                                                                                            >  4:469377/1‑149 (MQ=255)
          cAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAt                                                                                                                                        <  3:33007/149‑1 (MQ=255)
            gCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCAAAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttc                                                                                                                                      <  4:634241/149‑1 (MQ=255)
             ccaccaCGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAacgac                                                                                                                                                   <  3:975215/135‑1 (MQ=255)
              caccacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttctt                                                                                                                                    <  1:1039102/149‑1 (MQ=255)
              caccacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttctt                                                                                                                                    <  3:469377/149‑1 (MQ=255)
               accacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttcttc                                                                                                                                   >  2:183468/1‑149 (MQ=255)
                ccacgccacgATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTcc                                                                                                                                  >  3:716474/1‑149 (MQ=255)
                  acgccacgATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCAt                                                                                                                                >  3:141029/1‑149 (MQ=255)
                        cgATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGa                                                                                                                          <  2:797371/149‑1 (MQ=255)
                            aGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCAAAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                      <  1:53192/149‑1 (MQ=255)
                                   accgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGaaa                                                                                                               <  2:1392404/149‑1 (MQ=255)
                                          gTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCaaa                                                                                                        <  1:959476/149‑1 (MQ=255)
                                              cccGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttctt                                                                                                                                    >  3:811970/1‑117 (MQ=255)
                                                      cccACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgc                                                                                            >  2:229196/1‑149 (MQ=255)
                                                       ccaccaCGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgcg                                                                                           >  4:1115417/1‑149 (MQ=255)
                                                        caccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCa                                                                                                                                 <  2:404865/110‑1 (MQ=255)
                                                        caccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCa                                                                                                                                 >  1:404865/1‑110 (MQ=255)
                                                         accacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                         >  3:207036/1‑149 (MQ=255)
                                                         accacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                         >  2:676544/1‑149 (MQ=255)
                                                          ccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                        <  1:1262369/149‑1 (MQ=255)
                                                           cacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCaga                                                                                       <  1:109398/149‑1 (MQ=255)
                                                              gCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCa                                                                                    >  4:196053/1‑149 (MQ=255)
                                                              gCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCa                                                                                    >  2:600633/1‑149 (MQ=255)
                                                               ccAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAt                                                                                   >  4:672936/1‑149 (MQ=255)
                                                                cAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                  <  1:19337/149‑1 (MQ=255)
                                                                     ggCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAgcg                                                                             >  4:518223/1‑149 (MQ=255)
                                                                          ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATc                                                                                                                               >  3:1008662/1‑94 (MQ=255)
                                                                          ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                  >  3:26898/1‑139 (MQ=255)
                                                                          ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAAc                                                                        <  1:229196/149‑1 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCgg                                                                                                                            >  1:49288/1‑93 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCgg                                                                                                                            <  2:49288/93‑1 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCaa                                                                                                         >  4:969636/1‑112 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCa                                                                                                          >  1:1295929/1‑111 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                    <  1:600633/149‑1 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                    >  4:593558/1‑149 (MQ=255)
                                                                              tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                    >  3:1209984/1‑149 (MQ=255)
                                                                               ctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGa                                                                   >  4:1291830/1‑149 (MQ=255)
                                                                                ttttCCAAAGTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                                  <  4:716474/146‑1 (MQ=255)
                                                                                ttATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                                  <  4:207036/149‑1 (MQ=255)
                                                                                   tCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACg                                                                                                             >  4:78348/1‑103 (MQ=255)
                                                                                      aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCt                                                              <  4:1064979/147‑1 (MQ=255)
                                                                                      aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCt                                                              >  3:1064979/1‑147 (MQ=255)
                                                                                      aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                            <  1:676544/149‑1 (MQ=255)
                                                                                      aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                            >  4:46958/1‑149 (MQ=255)
                                                                                        aaTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgc                                                                                              <  4:395257/113‑1 (MQ=255)
                                                                                            gACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaa                                                      >  4:1124415/1‑149 (MQ=255)
                                                                                            gACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaa                                                      >  2:563979/1‑149 (MQ=255)
                                                                                              cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                  <  1:899689/119‑1 (MQ=255)
                                                                                              cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                  >  2:899689/1‑119 (MQ=255)
                                                                                              cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAAc                                                    <  3:1096982/149‑1 (MQ=255)
                                                                                                cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                  <  4:1209984/149‑1 (MQ=255)
                                                                                                 gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                 <  1:1399164/149‑1 (MQ=255)
                                                                                                 gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                 >  2:757982/1‑149 (MQ=255)
                                                                                                 gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                 >  4:935393/1‑149 (MQ=255)
                                                                                                 gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                 <  1:766981/149‑1 (MQ=255)
                                                                                                  ccATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATa                                                                                                                        <  2:1279986/77‑1 (MQ=255)
                                                                                                   cATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgta                                               <  4:263229/149‑1 (MQ=255)
                                                                                                       aTCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACggg                                                                     <  1:338416/123‑1 (MQ=255)
                                                                                                       aTCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACggg                                                                     >  2:338416/1‑123 (MQ=255)
                                                                                                        tCCCAGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                           <  4:1379632/132‑1 (MQ=255)
                                                                                                           cTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgtt                                       >  1:981229/1‑149 (MQ=255)
                                                                                                            tGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCaa                                                                         <  4:295002/114‑1 (MQ=255)
                                                                                                                aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttc                                                                                                                                      >  2:989/1‑49 (MQ=255)
                                                                                                                aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                         <  3:672736/94‑1 (MQ=255)
                                                                                                                aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                                 <  3:261196/118‑1 (MQ=255)
                                                                                                                aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                  >  4:1000744/1‑133 (MQ=255)
                                                                                                                aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                                >  1:304824/1‑135 (MQ=255)
                                                                                                                aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                                <  2:304824/135‑1 (MQ=255)
                                                                                                                  cAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                 >  2:1255701/1‑132 (MQ=255)
                                                                                                                  cAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                 <  1:1255701/132‑1 (MQ=255)
                                                                                                                   aaaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                  <  1:1015589/130‑1 (MQ=255)
                                                                                                                   aaaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                  >  2:1015589/1‑130 (MQ=255)
                                                                                                                    aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatc                              <  3:46958/149‑1 (MQ=255)
                                                                                                                     acgacgCGTGTTACAACTGCCGTCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGTGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                             <  1:1254005/149‑1 (MQ=255)
                                                                                                                     acgacgCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                             <  2:125470/149‑1 (MQ=255)
                                                                                                                       gacgCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGc                           >  1:1236580/1‑149 (MQ=255)
                                                                                                                            gtgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                           >  2:1357708/1‑112 (MQ=255)
                                                                                                                            gtgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                           <  1:1357708/112‑1 (MQ=255)
                                                                                                                             tgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCt                     <  3:136384/149‑1 (MQ=255)
                                                                                                                             tgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCt                     <  4:830239/149‑1 (MQ=255)
                                                                                                                             tgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCt                     <  2:1185040/149‑1 (MQ=255)
                                                                                                                               ttACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                                     <  3:1304129/131‑1 (MQ=255)
                                                                                                                                 aCAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                     >  2:978330/1‑113 (MQ=255)
                                                                                                                                 aCAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                             <  4:1447723/137‑1 (MQ=255)
                                                                                                                                   aaCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCTTTGAGCGCCAACGGGGAAGGCtt                                                             >  3:74898/1‑103 (MQ=255)
                                                                                                                                      tGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTaaa            <  3:196053/149‑1 (MQ=255)
                                                                                                                                      atccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                            >  4:1251995/3‑101 (MQ=255)
                                                                                                                                       tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                    <  3:1119331/91‑1 (MQ=255)
                                                                                                                                       tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAt                                          <  3:958457/117‑1 (MQ=255)
                                                                                                                                       tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAAt           >  3:175654/2‑149 (MQ=255)
                                                                                                                                       gccgccGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCt                                                              <  3:92137/98‑1 (MQ=255)
                                                                                                                                       gccgccGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                     >  3:1274306/1‑107 (MQ=255)
                                                                                                                                       gccgccGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAAt           <  3:518223/149‑1 (MQ=255)
                                                                                                                                              aaCGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAacac    >  1:747663/1‑149 (MQ=255)
                                                                                                                                              aaCGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAacac    <  3:593558/149‑1 (MQ=255)
                                                                                                                                                cgacgTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgta                                               <  3:1332392/104‑1 (MQ=255)
                                                                                                                                                cgacgTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgta                                               >  4:1332392/1‑104 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGC  >  NC_000913/3630515‑3630807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: