Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,630,667 A→G 6.7% intergenic (‑65/+301) yhiI ← / ← yhiJ putative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,630,6670AG 6.7% 596.6 / 22.6 178intergenic (‑65/+301)yhiI/yhiJputative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base A (82/84);  new base G (7/5);  total (89/89)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

Only 100 of 181 total aligned reads displayed.

GCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCAT  >  NC_000913/3630521‑3630815
                                                                                                                                                  |                                                                                                                                                    
        caccacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttctt                                                                                                                                            <  1:1039102/149‑1 (MQ=255)
         accacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttcttc                                                                                                                                           >  2:183468/1‑149 (MQ=255)
             cgccacgATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATc                                                                                                                                       <  4:141029/149‑1 (MQ=255)
                cacgATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCgg                                                                                                                                    <  3:1360578/149‑1 (MQ=255)
                  cgATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGa                                                                                                                                  <  2:797371/149‑1 (MQ=255)
                      aGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCAAAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                              <  1:53192/149‑1 (MQ=255)
                           ccaccgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGa                                                                                                                         >  2:959476/1‑149 (MQ=255)
                             accgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGaaa                                                                                                                       <  2:1392404/149‑1 (MQ=255)
                                    gTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCaaa                                                                                                                <  1:959476/149‑1 (MQ=255)
                                                cccACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgc                                                                                                    >  2:229196/1‑149 (MQ=255)
                                                   accacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                                 >  3:207036/1‑149 (MQ=255)
                                                   accacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                                 >  4:1096982/1‑149 (MQ=255)
                                                   accacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                                 >  2:676544/1‑149 (MQ=255)
                                                    ccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                                <  1:1262369/149‑1 (MQ=255)
                                                     cacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCaga                                                                                               <  1:109398/149‑1 (MQ=255)
                                                     cacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGAGATAAGAAGGAAACGGCAAATACGACCAGCGCGCaga                                                                                               >  4:33399/1‑149 (MQ=255)
                                                        gCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCa                                                                                            >  2:600633/1‑149 (MQ=255)
                                                          cAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                          <  1:19337/149‑1 (MQ=255)
                                                                    ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATc                                                                                                                                       <  4:1008662/94‑1 (MQ=255)
                                                                    ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATc                                                                                                                                       >  3:1008662/1‑94 (MQ=255)
                                                                    ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                          <  4:26898/139‑1 (MQ=255)
                                                                    ttACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAAc                                                                                <  1:229196/149‑1 (MQ=255)
                                                                        tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCaa                                                                                                                 <  3:969636/112‑1 (MQ=255)
                                                                        tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCaa                                                                                                                 >  4:969636/1‑112 (MQ=255)
                                                                        tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCa                                                                                                                  >  1:1295929/1‑111 (MQ=255)
                                                                        tctTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                            <  1:600633/149‑1 (MQ=255)
                                                                          ttttCCAAAGTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                                          <  4:716474/146‑1 (MQ=255)
                                                                          ttATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                                          <  4:207036/149‑1 (MQ=255)
                                                                            aTCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAgg                                                                        >  4:1129260/1‑149 (MQ=255)
                                                                             tCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACg                                                                                                                     <  3:78348/103‑1 (MQ=255)
                                                                               cATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                                >  1:368050/1‑122 (MQ=255)
                                                                               cATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                                <  2:368050/122‑1 (MQ=255)
                                                                                aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCt                                                                      >  3:1064979/1‑147 (MQ=255)
                                                                                aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                                    >  4:46958/1‑149 (MQ=255)
                                                                                aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                                    <  1:676544/149‑1 (MQ=255)
                                                                                  aaTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgc                                                                                                      >  3:395257/1‑113 (MQ=255)
                                                                                   aTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCTGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                                         >  2:409606/1‑141 (MQ=255)
                                                                                   aTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCTGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                                         <  1:409606/141‑1 (MQ=255)
                                                                                   aTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTg                                                                 >  3:263229/1‑149 (MQ=255)
                                                                                   aTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTg                                                                 >  3:57729/1‑149 (MQ=255)
                                                                                   aTCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTg                                                                 >  3:830239/1‑149 (MQ=255)
                                                                                      gACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaa                                                              >  2:563979/1‑149 (MQ=255)
                                                                                        cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCAtt                                                                                          <  1:899689/119‑1 (MQ=255)
                                                                                        cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAAc                                                            <  3:33399/149‑1 (MQ=255)
                                                                                           gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                         <  1:766981/149‑1 (MQ=255)
                                                                                           gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                         >  2:757982/1‑149 (MQ=255)
                                                                                           gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                         >  4:935393/1‑149 (MQ=255)
                                                                                           gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                         <  1:1399164/149‑1 (MQ=255)
                                                                                            ccATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATa                                                                                                                                <  2:1279986/77‑1 (MQ=255)
                                                                                                  tCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                                   >  3:1379632/1‑132 (MQ=255)
                                                                                                     cTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgtt                                               >  1:981229/1‑149 (MQ=255)
                                                                                                      tGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCaa                                                                                 >  3:295002/1‑114 (MQ=255)
                                                                                                       gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                                             <  4:57729/149‑1 (MQ=255)
                                                                                                        tAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtc                                            <  3:1115417/149‑1 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGAttc                                                                                                                                              >  2:989/1‑49 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCa                                                                                                 >  4:672736/1‑94 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                                         >  4:261196/1‑118 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                          >  4:1000744/1‑133 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                                        >  1:304824/1‑135 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                                        <  2:304824/135‑1 (MQ=255)
                                                                                                          aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcat                                          >  4:136384/1‑149 (MQ=255)
                                                                                                           gCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatc                                         <  3:672936/149‑1 (MQ=255)
                                                                                                           gCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatc                                         >  3:470715/1‑149 (MQ=255)
                                                                                                           gCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatc                                         <  3:1291830/149‑1 (MQ=255)
                                                                                                            cAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                         <  1:1255701/132‑1 (MQ=255)
                                                                                                            cAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                         >  2:1255701/1‑132 (MQ=255)
                                                                                                             aaaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                          >  2:1015589/1‑130 (MQ=255)
                                                                                                             aaaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACgg                                                          <  1:1015589/130‑1 (MQ=255)
                                                                                                              aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatc                                      <  3:46958/149‑1 (MQ=255)
                                                                                                               acgacgCGTGTTACAACTGCCGTCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGTGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                                     <  1:1254005/149‑1 (MQ=255)
                                                                                                               acgacgCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                                     <  2:125470/149‑1 (MQ=255)
                                                                                                               acgacgCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                                     <  3:1129260/149‑1 (MQ=255)
                                                                                                                 gacgCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGc                                   >  4:4100/1‑149 (MQ=255)
                                                                                                                 gacgCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGc                                   >  1:1236580/1‑149 (MQ=255)
                                                                                                                      gtgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                                   <  1:1357708/112‑1 (MQ=255)
                                                                                                                      gtgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                                   >  2:1357708/1‑112 (MQ=255)
                                                                                                                       tgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCt                             <  2:1185040/149‑1 (MQ=255)
                                                                                                                       tgtTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCt                             <  4:830239/149‑1 (MQ=255)
                                                                                                                         ttACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                                             >  4:1304129/1‑131 (MQ=255)
                                                                                                                           aCAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                             >  2:978330/1‑113 (MQ=255)
                                                                                                                           aCAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcatca                                     >  3:1447723/1‑137 (MQ=255)
                                                                                                                            cAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCg                        >  3:230361/1‑149 (MQ=255)
                                                                                                                            aagaatccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAt                                                  >  3:604891/7‑123 (MQ=255)
                                                                                                                            aagaatccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAt                                                  <  4:604891/117‑1 (MQ=255)
                                                                                                                             aaCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCTTTGAGCGCCAACGGGGAAGGCtt                                                                     >  3:74898/1‑103 (MQ=255)
                                                                                                                             aaCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                                     <  4:74898/103‑1 (MQ=255)
                                                                                                                               aatccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCt                                                                      >  4:182767/4‑100 (MQ=255)
                                                                                                                               aatccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCt                                                                      <  3:182767/97‑1 (MQ=255)
                                                                                                                                tGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTaaa                    <  4:470715/149‑1 (MQ=255)
                                                                                                                                atccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                                    <  3:1251995/99‑1 (MQ=255)
                                                                                                                                atccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                                    >  4:1251995/3‑101 (MQ=255)
                                                                                                                                 tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                            <  3:1119331/91‑1 (MQ=255)
                                                                                                                                 tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACgggg                                                                            >  4:1119331/2‑92 (MQ=255)
                                                                                                                                 gccgccGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                             <  4:1274306/107‑1 (MQ=255)
                                                                                                                                 gccgccGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAAt                   <  3:935393/149‑1 (MQ=255)
                                                                                                                                        aaCGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAacac            >  1:747663/1‑149 (MQ=255)
                                                                                                                                          cgacgTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgta                                                       <  3:1332392/104‑1 (MQ=255)
                                                                                                                                          cgacgTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGc          >  2:547360/1‑149 (MQ=255)
                                                                                                                                              gTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATAc      <  1:563979/149‑1 (MQ=255)
                                                                                                                                                  aGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCAt  >  4:800955/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCAT  >  NC_000913/3630521‑3630815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: