Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,636,748 G→A 5.3% G221G (GGC→GGT yhiN ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,636,7480GA 5.3% 505.3 / 10.3 149G221G (GGC→GGTyhiNputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (70/71);  new base A (4/4);  total (74/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

GCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTA  >  NC_000913/3636600‑3636889
                                                                                                                                                    |                                                                                                                                             
gctgctCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTg                                                                                                                                               >  2:1192009/1‑149 (MQ=255)
 ctgctCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGc                                                                                                                                              <  4:1192017/149‑1 (MQ=255)
    ctCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGt                                                                                                                                           >  1:62003/1‑149 (MQ=255)
    ctCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGt                                                                                                                                           <  4:260678/149‑1 (MQ=255)
     tCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGtt                                                                                                                                          <  1:36944/149‑1 (MQ=255)
       aTTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGtttt                                                                                                                                        >  1:30317/1‑149 (MQ=255)
            ggAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCg                                                                                                                                   >  4:93624/1‑149 (MQ=255)
                       aGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGcc                                                                                                                                             <  1:496634/128‑1 (MQ=255)
                       aGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGcc                                                                                                                                             >  2:496634/1‑128 (MQ=255)
                           ccACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGc                                                                                                                    >  3:1324880/1‑149 (MQ=255)
                              caTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCAcc                                                                                                                 >  2:501032/1‑149 (MQ=255)
                                tCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGc                                                                                                               >  3:686320/1‑149 (MQ=255)
                                     tAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACgg                                                                                                                         >  4:1018225/1‑134 (MQ=255)
                                     tAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACgg                                                                                                                         <  3:1018225/134‑1 (MQ=255)
                                      aGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGcc                                                                                                                                             >  4:542054/1‑113 (MQ=255)
                                       gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAgg                                                                                                                     >  3:976129/1‑136 (MQ=255)
                                       gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAgg                                                                                                                     <  4:976129/136‑1 (MQ=255)
                                                  cTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTg                                                                                             >  2:700688/1‑149 (MQ=255)
                                                    gACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgcccgcc                                                                                                     >  1:451724/1‑139 (MQ=255)
                                                     aCAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          >  4:757334/1‑149 (MQ=255)
                                                     aCAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          <  2:1067038/149‑1 (MQ=255)
                                                      cAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAAc                                                                                         <  4:1324880/149‑1 (MQ=255)
                                                       aaaTTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAAct                                                                                        >  4:845925/1‑149 (MQ=255)
                                                          ttCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTcc                                                                                                                                      <  4:1107154/100‑2 (MQ=255)
                                                          ttCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTcc                                                                                                                                      >  3:1107154/1‑99 (MQ=255)
                                                            cccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCg                                                                                   <  1:702044/149‑1 (MQ=255)
                                                            cccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCg                                                                                   <  3:578288/149‑1 (MQ=255)
                                                             ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                  <  3:937964/149‑1 (MQ=255)
                                                             ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                  >  3:61200/1‑149 (MQ=255)
                                                              cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActct                                                                                      <  4:981215/144‑1 (MQ=255)
                                                              cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActct                                                                                      <  3:739594/144‑1 (MQ=255)
                                                              cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActct                                                                                      >  4:739594/1‑144 (MQ=255)
                                                                  gTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAAc                                                                             <  4:61200/149‑1 (MQ=255)
                                                                        aGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTa                                                                       >  3:660264/1‑149 (MQ=255)
                                                                         gTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          <  1:573440/129‑1 (MQ=255)
                                                                         gTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          >  2:573440/1‑129 (MQ=255)
                                                                            gCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCa                                                                   >  1:1020411/1‑149 (MQ=255)
                                                                              ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATc                                                                                                                             <  1:819888/89‑1 (MQ=255)
                                                                              ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                 >  1:646255/1‑149 (MQ=255)
                                                                              ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                 >  2:448782/1‑149 (MQ=255)
                                                                               tGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCagag                                                                <  3:1245757/149‑1 (MQ=255)
                                                                                   aTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGaa                                                            <  1:459848/149‑1 (MQ=255)
                                                                                    tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGttt                                                                        <  1:181490/136‑1 (MQ=255)
                                                                                    tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGttt                                                                        >  2:181490/1‑136 (MQ=255)
                                                                                    tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAAt                                                           <  3:757334/149‑1 (MQ=255)
                                                                                      tGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTa                                                                       >  2:1251243/1‑135 (MQ=255)
                                                                                      tGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATgg                                                         <  2:30317/149‑1 (MQ=255)
                                                                                        cAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCa                                                       <  2:646255/149‑1 (MQ=255)
                                                                                         aaCACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          <  3:98836/113‑1 (MQ=255)
                                                                                         aaCACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCAc                                                      <  4:686320/149‑1 (MQ=255)
                                                                                          acacCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGaa                                                            >  4:418087/1‑142 (MQ=255)
                                                                                           cacCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          <  1:40042/111‑1 (MQ=255)
                                                                                           cacCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          >  2:40042/1‑111 (MQ=255)
                                                                                           cacCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCa                                                    >  3:1014496/1‑149 (MQ=255)
                                                                                            acCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                 >  1:1318325/1‑135 (MQ=255)
                                                                                            acCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                 <  2:1318325/135‑1 (MQ=255)
                                                                                                    tCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                  <  3:234874/110‑1 (MQ=255)
                                                                                                        gACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCAcc                                                     >  2:56261/1‑135 (MQ=255)
                                                                                                        gACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCAcc                                                     <  1:56261/135‑1 (MQ=255)
                                                                                                        gACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGt                                       >  4:1072875/1‑149 (MQ=255)
                                                                                                         aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTc                                      <  1:501032/149‑1 (MQ=255)
                                                                                                            aGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          <  4:89433/94‑1 (MQ=255)
                                                                                                            aGCCGCGGTGAGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          >  3:89433/1‑94 (MQ=255)
                                                                                                             gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCtt                                                                                     <  4:1008601/98‑1 (MQ=255)
                                                                                                             gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                  <  4:1420852/149‑5 (MQ=255)
                                                                                                             gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgca                                  >  1:1027413/1‑149 (MQ=255)
                                                                                                             gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgca                                  <  1:1192009/149‑1 (MQ=255)
                                                                                                              ccGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgcag                                 >  2:348935/1‑149 (MQ=255)
                                                                                                                       gtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgc                                           <  3:720393/130‑1 (MQ=255)
                                                                                                                         gAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGc                      >  4:624094/1‑149 (MQ=255)
                                                                                                                            gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATc                                                                                                                             >  4:400355/1‑43 (MQ=255)
                                                                                                                            gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATc                                                                                                                             <  3:400355/43‑1 (MQ=255)
                                                                                                                            gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGATTGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                   >  4:1198917/1‑149 (MQ=255)
                                                                                                                            gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                  <  2:1308626/134‑5 (MQ=255)
                                                                                                                            gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                  >  1:1308626/1‑130 (MQ=255)
                                                                                                                            gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                   >  3:754057/1‑149 (MQ=255)
                                                                                                                               tAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACg                                                                            <  3:421048/89‑1 (MQ=255)
                                                                                                                                  gTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                  >  1:1104760/1‑80 (MQ=255)
                                                                                                                                  gTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                  >  2:839152/1‑80 (MQ=255)
                                                                                                                                  gTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                  <  2:985796/128‑5 (MQ=255)
                                                                                                                                  gTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggt                                  >  1:985796/1‑124 (MQ=255)
                                                                                                                                   ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTACCTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                         >  4:472733/1‑136 (MQ=255)
                                                                                                                                   ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                 >  2:203435/1‑96 (MQ=255)
                                                                                                                                   ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                 <  1:203435/96‑1 (MQ=255)
                                                                                                                                   ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                           <  3:873040/134‑1 (MQ=255)
                                                                                                                                   ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                         <  3:472733/136‑1 (MQ=255)
                                                                                                                                   ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTc            <  1:348935/149‑1 (MQ=255)
                                                                                                                                      tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          <  1:159324/68‑1 (MQ=255)
                                                                                                                                      tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTaa                                                                                          >  2:159324/1‑68 (MQ=255)
                                                                                                                                        aCGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCagag                                                                <  4:190132/92‑1 (MQ=255)
                                                                                                                                        aCGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCagag                                                                >  3:190132/1‑92 (MQ=255)
                                                                                                                                        aCGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCaa       >  3:36144/1‑149 (MQ=255)
                                                                                                                                          tgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                          >  1:159065/2‑112 (MQ=255)
                                                                                                                                          tgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                          >  3:1092588/2‑112 (MQ=255)
                                                                                                                                          tgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                          <  4:1092588/111‑1 (MQ=255)
                                                                                                                                          tgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                          <  2:159065/111‑1 (MQ=255)
                                                                                                                                          ggAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGt              <  4:689882/140‑1 (MQ=255)
                                                                                                                                          ggAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc     >  3:786880/1‑149 (MQ=255)
                                                                                                                                           gAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                           >  3:787339/1‑126 (MQ=255)
                                                                                                                                           gAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                           <  4:787339/126‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTA  >  NC_000913/3636600‑3636889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: