| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,642,361 | T→A | 22.2% | intergenic (+30/+19) | dtpB → / ← rsmJ | dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,642,361 | 0 | T | A | 22.2% | 118.5 / 33.4 | 54 | intergenic (+30/+19) | dtpB/rsmJ | dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (19/23); new base A (6/6); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01 | |||||||||||
GCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAG > NC_000913/3642214‑3642509 | gCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATc < 4:1308945/149‑1 (MQ=255)gCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATc < 2:54989/149‑1 (MQ=255) tCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTAc > 4:572132/1‑149 (MQ=255) tCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTAc < 4:388043/149‑1 (MQ=255) tataCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTAccc > 3:270929/1‑149 (MQ=255) ataCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCt < 4:210901/149‑1 (MQ=255) ataCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt < 4:270929/149‑1 (MQ=255) ataCCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt < 3:13351/149‑1 (MQ=255) cAACGTGTTTAGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGTATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCTGAAAGCAGGGTAGCGTTATCGCTACCCTgttt < 4:1253901/149‑1 (MQ=255) aGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttt < 4:549491/136‑1 (MQ=255) aGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgtttt > 3:549491/1‑136 (MQ=255) aTTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAACAGGGTAGCGATAACGCTAcc > 1:1103232/1‑127 (MQ=255) aTTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAACAGGGTAGCGATAACGCTAcc < 2:1103232/127‑1 (MQ=255) gtctggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTAt > 1:5841/1‑116 (MQ=255) gtctggtcACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTAt < 2:5841/116‑1 (MQ=255) gtGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTAccc < 4:416965/98‑1 (MQ=255) gtGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTAccc > 3:416965/1‑98 (MQ=255) tGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt < 3:572132/149‑1 (MQ=255) cTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtt > 1:849670/1‑84 (MQ=255) cTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttttagtt < 2:849670/84‑1 (MQ=255) tGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttt > 4:1112951/1‑78 (MQ=255) tGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTgttt < 3:1112951/78‑1 (MQ=255) aaCGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGAc > 3:289865/1‑149 (MQ=255) gCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt > 1:645368/1‑68 (MQ=38) gCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGATAACGCTACCCt < 2:645368/68‑1 (MQ=38) gACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt < 4:754382/112‑1 (MQ=255) gACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGt > 3:754382/1‑112 (MQ=255) gAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGt < 4:289865/149‑1 (MQ=255) ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCttt > 1:1442552/1‑94 (MQ=255) ttaattaTTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCttt < 2:1442552/94‑1 (MQ=255) attattCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGgc < 1:1035624/134‑1 (MQ=255) attattCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGgc > 2:1035624/1‑134 (MQ=255) ttCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATc > 3:254011/1‑72 (MQ=255) ttCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATc < 4:254011/72‑1 (MQ=255) gCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGc > 4:291598/1‑115 (MQ=255) gCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGc < 3:291598/115‑1 (MQ=255) gCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtg > 2:1313238/1‑97 (MQ=255) gCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtggcgtg < 1:1313238/97‑1 (MQ=255) gCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGtt > 2:327427/1‑149 (MQ=255) ggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt < 2:1320573/86‑1 (MQ=255) ggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt > 1:1320573/1‑86 (MQ=255) ggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt > 2:119/1‑86 (MQ=255) ggTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGtt < 1:119/86‑1 (MQ=255) gTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTa > 3:693821/1‑118 (MQ=255) gTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTa < 4:693821/118‑1 (MQ=255) gTAGCGATAACGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTc > 2:956582/1‑149 (MQ=255) tAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCg > 4:1230910/1‑149 (MQ=255) gCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGttt < 2:1138255/142‑1 (MQ=255) gCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGttt > 1:1138255/1‑142 (MQ=255) gTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGt > 1:547080/1‑115 (MQ=255) gTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGt < 2:547080/115‑1 (MQ=255) ttATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtgg > 4:527161/1‑82 (MQ=255) ttATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTtgg < 3:527161/82‑1 (MQ=255) aTCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTg < 4:178830/103‑1 (MQ=255) aTCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTg > 3:178830/1‑103 (MQ=255) aTCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCaa < 1:327427/149‑1 (MQ=255) tCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATa < 2:932479/40‑1 (MQ=255) tCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATa > 1:932479/1‑40 (MQ=255) tCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAg > 3:587759/1‑149 (MQ=255) | GCCCGTCTATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAACGCATGATTGCGACGCCGGAAAGCCATTAATTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGCGATGCCCTTTAGTGACTACCGCGTTTGGCGTGGCGACATTCGCCAGCGGTGGCGCGTAGTCCGGGCGCTTTACCACCACGCGTTTGGTCGCCAGCAAG > NC_000913/3642214‑3642509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |