| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,668,183 | T→A | 7.5% | T336S (ACG→TCG) ‡ | yhjA ← | putative cytochrome C peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,668,183 | 0 | T | A | 7.5% | 183.6 / 4.9 | 53 | T336S (ACG→TCG) ‡ | yhjA | putative cytochrome C peroxidase |
| Reads supporting (aligned to +/- strand): ref base T (25/24); new base A (2/2); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
AAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTT > NC_000913/3668067‑3668321 | aaaGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCAt > 1:1179394/1‑145 (MQ=255)aaaGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCAt < 2:1179394/145‑1 (MQ=255) tCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCaaa > 1:451475/1‑149 (MQ=255) ttttttCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCAtttt > 4:725396/1‑122 (MQ=255) ttttttCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCAtttt < 3:725396/122‑1 (MQ=255) tttttCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATgg < 2:451475/149‑1 (MQ=255) cAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCAtttt > 3:116890/1‑116 (MQ=255) cAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCAtttt < 4:116890/116‑1 (MQ=255) cccAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAgc > 2:790018/1‑105 (MQ=255) cccAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAgc < 1:790018/105‑1 (MQ=255) ccAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGc > 3:462125/1‑149 (MQ=255) gAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATggg < 3:171767/126‑1 (MQ=255) gAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATggg > 4:171767/1‑126 (MQ=255) gAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGtt > 1:1025140/1‑147 (MQ=255) gAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGtt < 2:1025140/147‑1 (MQ=255) cGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtctc < 4:462125/149‑1 (MQ=255) gTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTa < 4:1407973/113‑1 (MQ=255) gTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTa > 3:1407973/1‑113 (MQ=255) gTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCa > 2:318668/1‑149 (MQ=255) aataatACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCaa < 1:318668/149‑1 (MQ=255) ataCCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtc > 1:29294/1‑132 (MQ=255) ataCCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtc < 2:29294/132‑1 (MQ=255) caccaTGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtct > 3:315121/1‑129 (MQ=255) caccaTGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtct < 4:315121/129‑1 (MQ=255) caTGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCcgc < 3:102571/81‑1 (MQ=255) caTGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCcgc > 4:102571/1‑81 (MQ=255) aCAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCcgc < 3:172017/77‑1 (MQ=255) aCAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCcgc > 4:172017/1‑77 (MQ=255) aaGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCaaa < 1:1180225/124‑1 (MQ=255) aaGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCaaa > 2:1180225/1‑124 (MQ=255) ttGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCAtt > 4:772470/1‑61 (MQ=255) ttGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCAtt < 3:772470/61‑1 (MQ=255) ttatCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGtaattaat < 4:298684/99‑1 (MQ=255) ttatCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGtaattaat > 3:298684/1‑99 (MQ=255) tttAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGtaattaa < 1:1029312/93‑1 (MQ=255) tttAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGtaattaa > 2:1029312/1‑93 (MQ=255) ataatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCaaa < 1:1009174/67‑1 (MQ=255) ataatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCaaa > 2:1009174/1‑67 (MQ=255) ataatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGc > 2:1396374/1‑143 (MQ=255) ataatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGc < 1:1396374/143‑1 (MQ=255) atTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGAt < 2:214482/144‑1 (MQ=255) atTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGAt > 1:214482/1‑144 (MQ=255) ccTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGttt > 2:987468/1‑82 (MQ=255) ccTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGttt < 1:987468/82‑1 (MQ=255) cTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAAc > 3:1339572/1‑149 (MQ=255) ttttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtc > 2:811156/1‑82 (MQ=255) ttttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtc < 1:811156/82‑1 (MQ=255) ttttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtc < 2:1306862/82‑1 (MQ=255) ttttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTtc > 1:1306862/1‑82 (MQ=255) aaatGCGCTGACGGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGtttt > 3:1370186/4‑149 (MQ=255) aatGCGCTGACGGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTc > 1:24505/3‑106 (MQ=255) aatGCGCTGACGGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTc < 2:24505/104‑1 (MQ=255) aatGCGCTGACGGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGaaa < 2:644159/114‑1 (MQ=255) tCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATggg > 3:509694/1‑41 (MQ=255) tCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATggg < 4:509694/41‑1 (MQ=255) | AAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTT > NC_000913/3668067‑3668321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |