Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,985 T→C 7.3% intergenic (‑195/+113) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9850TC 7.3% 181.4 / 4.0 55intergenic (‑195/+113)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (24/27);  new base C (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTG  >  NC_000913/3705839‑3706131
                                                                                                                                                  |                                                                                                                                                  
ttGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                                                                  <  3:1103587/149‑1 (MQ=255)
ttGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                                                                  <  2:808309/149‑1 (MQ=255)
        agaTCAATTCCCGCCCTTATCGGACAGGAAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                                                            <  1:178314/145‑2 (MQ=38)
        aaaTCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                                                            >  2:178314/1‑146 (MQ=255)
         aaTCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                                                            >  1:1116203/1‑146 (MQ=255)
         aaTCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                                                            <  2:1116203/146‑1 (MQ=255)
          aTCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                                                              <  3:839850/143‑1 (MQ=255)
          aTCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                                                              >  4:839850/1‑143 (MQ=255)
                          aTCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                                                                        <  4:177921/149‑1 (MQ=255)
                                          aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAttt                                                                                                        >  1:1105573/1‑149 (MQ=255)
                                               tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                                                                                                   <  1:818782/149‑1 (MQ=255)
                                               tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                                                                                                   <  2:1105573/149‑1 (MQ=255)
                                                                gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                                                                                      <  3:1368908/129‑1 (MQ=255)
                                                                gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                                                                                      >  4:1368908/1‑129 (MQ=255)
                                                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                                                                       >  4:1045044/1‑110 (MQ=255)
                                                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                                                                       <  3:1045044/110‑1 (MQ=255)
                                                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACa                                                                                                                   <  1:398377/114‑1 (MQ=255)
                                                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACa                                                                                                                   >  2:398377/1‑114 (MQ=255)
                                                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                                                           <  2:1134534/103‑1 (MQ=255)
                                                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                                                           >  1:1134534/1‑103 (MQ=255)
                                                                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGTCCTGATAAGCGGAGCGCATCAGGCa                                                                        >  3:8418/1‑149 (MQ=255)
                                                                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGAt                                                                                           <  3:304966/126‑1 (MQ=255)
                                                                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGAt                                                                                           >  4:304966/1‑126 (MQ=255)
                                                                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                                                                     <  1:188617/94‑1 (MQ=255)
                                                                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                                                                     >  2:188617/1‑94 (MQ=255)
                                                                                           ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCGATACATTGCAGAATTTTCGTCGGc                                                                                                >  1:211336/1‑108 (MQ=255)
                                                                                           ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGc                                                                                                <  2:211336/108‑1 (MQ=255)
                                                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATc                                                                             >  3:183843/1‑126 (MQ=255)
                                                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATc                                                                             <  4:183843/126‑1 (MQ=255)
                                                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa                                                      <  4:8418/149‑1 (MQ=255)
                                                                                                       aaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAGTCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGa                                                                                    <  2:1443123/107‑1 (MQ=255)
                                                                                                       aaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGa                                                                                    >  1:1443123/2‑108 (MQ=255)
                                                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt                                                                                              <  2:626420/97‑1 (MQ=255)
                                                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt                                                                                              >  1:1163117/1‑97 (MQ=255)
                                                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt                                                                                              <  2:1163117/97‑1 (MQ=255)
                                                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt                                                                                              >  1:626420/1‑97 (MQ=255)
                                                                                                             gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                                                                     <  2:723122/69‑1 (MQ=39)
                                                                                                             gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                                                                     >  1:723122/1‑69 (MQ=40)
                                                                                                                   tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATa                                                                                          <  2:391831/90‑1 (MQ=255)
                                                                                                                   tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATa                                                                                          >  1:391831/1‑90 (MQ=255)
                                                                                                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc                                                                                  <  4:934957/92‑1 (MQ=255)
                                                                                                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc                                                                                  >  3:934957/1‑92 (MQ=255)
                                                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa                                                      >  3:215897/1‑116 (MQ=255)
                                                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa                                                      <  4:215897/116‑1 (MQ=255)
                                                                                                                               ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                       <  1:952280/97‑1 (MQ=255)
                                                                                                                               ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                       >  2:952280/1‑97 (MQ=255)
                                                                                                                                 ataaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                                         >  4:284107/5‑125 (MQ=255)
                                                                                                                                 ataaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                                         <  3:284107/121‑1 (MQ=255)
                                                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa                          <  2:643058/138‑1 (MQ=255)
                                                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa                          >  1:643058/1‑138 (MQ=255)
                                                                                                                                              gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg                                                                           >  2:1266485/1‑78 (MQ=255)
                                                                                                                                              gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg                                                                           <  1:1266485/78‑1 (MQ=255)
                                                                                                                                                cTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                                                                  <  4:180037/37‑1 (MQ=255)
                                                                                                                                                cTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                                                                  >  3:180037/1‑37 (MQ=255)
                                                                                                                                                cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTg  >  1:1040907/1‑149 (MQ=255)
                                                                                                                                                 ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGttt   <  2:289544/147‑1 (MQ=255)
                                                                                                                                                 ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGttt   >  1:289544/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTGCTCACAAATCAATTCCCGCCCTTATCGGACAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTG  >  NC_000913/3705839‑3706131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: