Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,736,322 G→T 10.2% intergenic (+165/+31) xylR → / ← bax xylose divergent operon transcriptional activator/putative glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,3220GT10.2% 753.7 / 52.3 224intergenic (+165/+31)xylR/baxxylose divergent operon transcriptional activator/putative glucosaminidase
Reads supporting (aligned to +/- strand):  ref base G (103/98);  new base T (11/12);  total (114/110)
Fisher's exact test for biased strand distribution p-value = 8.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 225 total aligned reads displayed.

GGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTC  >  NC_000913/3736175‑3736470
                                                                                                                                                    |                                                                                                                                                    
 gCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                    <  4:1061905/149‑1 (MQ=255)
 gCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                    <  4:261866/149‑1 (MQ=255)
        aTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCGTGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcgg                                                                                                                                             <  2:1448730‑M2/149‑7 (MQ=255)
        aTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcgg                                                                                                                                             >  4:59296‑M2/1‑143 (MQ=255)
             gTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataag                                                                                                                                        >  2:895156‑M2/1‑138 (MQ=255)
                aCGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         >  3:260686‑M2/1‑135 (MQ=255)
                  gCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagt                                                                                                                                                >  1:1192797‑M2/1‑133 (MQ=255)
                  gCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagt                                                                                                                                                <  2:1192797‑M2/136‑4 (MQ=255)
                           ttGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         <  1:30144‑M2/134‑11 (MQ=255)
                                 gataaggcgttcacGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataag                                                                                                                                        >  3:745254‑M2/6‑118 (MQ=255)
                                          tGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAa                                                                                                                                                  <  3:675369‑M2/110‑2 (MQ=255)
                                          tGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAa                                                                                                                                                  >  4:675369‑M2/1‑109 (MQ=255)
                                           gAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAag                                                                                                                                                 <  3:754388‑M2/110‑3 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         >  1:5182‑M2/1‑104 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtc                                                                                                                                               >  3:920881‑M2/1‑104 (MQ=255)
                                                ccTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         >  2:781660‑M2/1‑103 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                    >  4:315162/1‑101 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcgg                                                                                                                                             >  4:1016988‑M2/1‑102 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcgg                                                                                                                                             <  3:1016988‑M2/108‑7 (MQ=255)
                                                     tCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCg                                                                                                >  4:515541/1‑149 (MQ=255)
                                                        gACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         >  3:868182‑M2/1‑95 (MQ=255)
                                                            tACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAgtcggataa                                                                                                                                          >  3:1188699‑M2/1‑92 (MQ=255)
                                                            tACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                    <  2:1359084/90‑1 (MQ=255)
                                                            tACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTa                                                                                         <  4:886136/149‑1 (MQ=255)
                                                             aCCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtc                                                                                                                                               >  1:143055‑M2/1‑90 (MQ=255)
                                                             aCCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTAt                                                                                        >  1:1145445/1‑149 (MQ=255)
                                                                          gATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                             >  2:101453/1‑131 (MQ=255)
                                                                          gATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                             <  1:101453/131‑1 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         <  1:1213607‑M2/86‑11 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                    >  2:1047835/1‑75 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                    <  1:1047835/75‑1 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACAGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         >  2:1213607‑M2/1‑76 (MQ=255)
                                                                            tAAAACGCACCAACAACGCTTCAGGCTCGTACCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggata                                                                                                                                          >  4:921166‑M2/1‑75 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACAtt                                                                             <  3:806837/142‑1 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGATCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                         >  3:500658‑M2/1‑72 (MQ=255)
                                                                                    aCCAACAACGCTTCAGGCTCGTAGCTCAACTGGCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGAGAAGGCTCAAGGAAATGCaa                                                                                                                        <  4:153525/94‑1 (MQ=255)
                                                                                    aCCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggat                                                                                                                                           >  3:4647‑M2/1‑67 (MQ=255)
                                                                                    aCCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCaa                                                                                                                        >  3:153525/1‑94 (MQ=255)
                                                                                       aacaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcg                                                                                                                                              <  4:894481‑M2/69‑6 (MQ=255)
                                                                                             gCTTCAGGCTCGTAGCTCAATTGCTTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCTAGGAAATGCAATTACATATGCGTCTCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATaa                                                        <  4:465556/149‑1 (MQ=255)
                                                                                             gCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATaa                                                        <  3:1422306/149‑1 (MQ=255)
                                                                                               ttCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                         <  2:379184‑M2/66‑11 (MQ=255)
                                                                                               ttCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCt                                                                                     >  1:699747/1‑118 (MQ=255)
                                                                                                 cAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAAt                                                                                                                            >  4:411256/1‑77 (MQ=255)
                                                                                                  aGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                         >  3:1348160‑M2/1‑53 (MQ=255)
                                                                                                  aGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                         <  4:1348160‑M2/63‑11 (MQ=255)
                                                                                                      tCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGcgccgc                                                                                                         >  4:780246/1‑91 (MQ=255)
                                                                                                        gTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCaa                                                                                                                                  <  2:674290/64‑1 (MQ=255)
                                                                                                        gTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTa                                                                                                                     >  3:645598/1‑77 (MQ=255)
                                                                                                        gTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTa                                                                                                                     <  4:645598/77‑1 (MQ=255)
                                                                                                         tAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTg                                            <  3:891831/149‑1 (MQ=255)
                                                                                                            cTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataag                                                                                                                                        <  3:771611‑M2/54‑12 (MQ=255)
                                                                                                             tCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAAc                                                                                                  >  1:461853/1‑91 (MQ=255)
                                                                                                             tCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                        >  3:540598/1‑117 (MQ=255)
                                                                                                                aCTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAtt                                                                                                     <  1:103712/85‑1 (MQ=255)
                                                                                                                aCTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAtt                                                                                                     >  2:103712/1‑85 (MQ=255)
                                                                                                                    ccTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGcc                                 >  3:1089230/1‑149 (MQ=255)
                                                                                                                       gagaCAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACa                                                                                                                   >  1:1310148/1‑64 (MQ=255)
                                                                                                                       gagaCAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACa                                                                                                                   <  2:1310148/64‑1 (MQ=255)
                                                                                                                          aCAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAg                           >  2:1150615/1‑149 (MQ=255)
                                                                                                                           cAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAActct                                                    <  2:1415015/123‑1 (MQ=255)
                                                                                                                            aaaGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGCTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGCAGCCCTTCAGCt                         >  4:285401/1‑149 (MQ=255)
                                                                                                                              aGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                        >  1:1406812/1‑100 (MQ=255)
                                                                                                                               gTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgtca                                                           >  4:109974/1‑110 (MQ=255)
                                                                                                                               gTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTta                                                           <  3:109974/112‑1 (MQ=255)
                                                                                                                                tAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTg                                            <  1:796842/126‑1 (MQ=255)
                                                                                                                                  aaaaGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAAc                                                       >  3:940142/1‑113 (MQ=255)
                                                                                                                                       ccTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTgg                                                                                            >  4:666666/1‑71 (MQ=255)
                                                                                                                                       ccTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTgg                                                                                            <  3:666666/71‑1 (MQ=255)
                                                                                                                                        cTTATCCGACTTGTCAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTg                                                                            <  1:1327109/86‑1 (MQ=38)
                                                                                                                                        cTTATCCGACTTGTCAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGATTATCCTGGTACATTg                                                                            >  2:1327109/1‑86 (MQ=37)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgtt                                                               <  4:1269219/99‑1 (MQ=255)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgtt                                                               >  3:1269219/1‑99 (MQ=255)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAActct                                                    >  2:541786/1‑110 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaaccacgcagcgttacc            <  3:354781‑M2/149‑136 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaa                           <  2:30144‑M2/134‑121 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataagg                                                <  4:1441845‑M2/113‑100 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaa                                                                                               <  1:379184‑M2/66‑53 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCTATTaa                                                                                                   <  2:416175/62‑1 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTaa                                                                                                   >  1:416175/1‑62 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgt                                                             >  2:308618/1‑100 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgt                                                             >  4:752227/1‑100 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAg                           <  4:1384548/134‑1 (MQ=255)
                                                                                                                                           aTCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatca                <  4:80534/143‑1 (MQ=255)
                                                                                                                                            tCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaaccacgcagcgttacctga         <  2:938289‑M2/149‑139 (MQ=255)
                                                                                                                                            tCCGACTGACAagtcggataaggctttttactttgtctcaggcagttga                                                                                                             >  3:305861‑M2/1‑11 (MQ=255)
                                                                                                                                            tCCGACTGACAagtcggataaggctttttactttgtctcaggcagt                                                                                                                <  3:782954‑M2/46‑36 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGc                                                                           >  2:469579/1‑83 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAg                                                                 <  4:855705/93‑1 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTtgt                      >  4:1166897/1‑136 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTtgt                      <  3:1166897/136‑1 (MQ=255)
                                                                                                                                             ccGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgtttcatcatgcctggcgggtaggtcggataaggcgttcacgc                                       >  1:173354‑M2/1‑10 (MQ=255)
                                                                                                                                               gACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGa                                                                         >  4:1232046/1‑82 (MQ=255)
                                                                                                                                               gACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGc      >  1:667130/1‑149 (MQ=255)
                                                                                                                                                 cTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaaccacgcagcgttacctgatgtga    >  1:938289‑M2/1‑6 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                        <  1:875688/80‑1 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                        >  2:875688/1‑80 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCtt                              <  4:757930/122‑1 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGt   >  3:1408671/1‑149 (MQ=255)
                                                                                                                                                    gACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGCTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGCAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTc  <  3:285401/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTC  >  NC_000913/3736175‑3736470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: