Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,736,323 A→G 10.4% intergenic (+166/+30) xylR → / ← bax xylose divergent operon transcriptional activator/putative glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,3230AG10.4% 672.6 / 47.9 222intergenic (+166/+30)xylR/baxxylose divergent operon transcriptional activator/putative glucosaminidase
Reads supporting (aligned to +/- strand):  ref base A (100/96);  new base G (11/12);  total (113/109)
Fisher's exact test for biased strand distribution p-value = 8.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 241 total aligned reads displayed.

GGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTCA  >  NC_000913/3736175‑3736471
                                                                                                                                                     |                                                                                                                                                    
ggCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGa                                                                                                                                                      <  3:1045669/149‑1 (MQ=255)
 gCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     <  4:1061905/149‑1 (MQ=255)
                 cGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtc                                                                                                                                                <  3:608298‑M2/138‑5 (MQ=255)
                 cGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtc                                                                                                                                                >  4:608298‑M2/1‑134 (MQ=255)
                                          tGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAa                                                                                                                                                   <  3:675369‑M2/110‑2 (MQ=255)
                                          tGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAa                                                                                                                                                   >  4:675369‑M2/1‑109 (MQ=255)
                                               gCCTTATCCGACCTACCCGTCAGGCATGATAAAACGCACCAACAACGCTTCATGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCTACTGACAagtc                                                                                                                                                <  4:920881‑M2/108‑5 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCGACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataag                                                                                                                                         <  1:425819‑M2/115‑12 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAgtcggataag                                                                                                                                          >  2:425819‑M2/1‑105 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                          >  1:5182‑M2/1‑104 (MQ=255)
                                               gCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                          <  2:5182‑M2/114‑11 (MQ=255)
                                                ccTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                          <  3:1441845‑M2/113‑11 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     <  3:315162/101‑1 (MQ=255)
                                                 cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     >  4:315162/1‑101 (MQ=255)
                                                            tACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     >  1:1359084/1‑90 (MQ=255)
                                                            tACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     <  2:1359084/90‑1 (MQ=255)
                                                            tACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTa                                                                                          <  4:886136/149‑1 (MQ=255)
                                                             aCCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTAt                                                                                         >  1:1145445/1‑149 (MQ=255)
                                                                        aTGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtc                                                                                                                                                <  2:878005‑M2/83‑5 (MQ=255)
                                                                          gATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTg                                                                                              <  1:101453/131‑1 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCagtcggataa                                                                                                                                          <  1:1213607‑M2/86‑11 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     >  2:1047835/1‑75 (MQ=255)
                                                                           aTAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                                                                                                                     <  1:1047835/75‑1 (MQ=255)
                                                                            tAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcgga                                                                                                                                             <  4:1177901‑M2/82‑8 (MQ=255)
                                                                            tAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACAGACAagtcgga                                                                                                                                             >  3:1177901‑M2/1‑75 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                          <  4:500658‑M2/82‑11 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                          <  4:654514‑M2/82‑11 (MQ=255)
                                                                               aaCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACAtt                                                                              <  3:806837/142‑1 (MQ=255)
                                                                                    aCCAACAACGCTTCAGGCTCGTAGCTCAACTGGCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGAGAAGGCTCAAGGAAATGCaa                                                                                                                         <  4:153525/94‑1 (MQ=255)
                                                                                    aCCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggat                                                                                                                                            <  4:4647‑M2/75‑9 (MQ=255)
                                                                                    aCCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCaa                                                                                                                         >  3:153525/1‑94 (MQ=255)
                                                                                       aacaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcg                                                                                                                                               >  3:894481‑M2/1‑64 (MQ=255)
                                                                                       aacaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTCATCCGACTGACAagtcggatga                                                                                                                                          <  2:408302‑M2/74‑11 (MQ=255)
                                                                                       aacaacGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTCATCCGACTGACAagtcggatga                                                                                                                                          >  1:408302‑M2/1‑64 (MQ=255)
                                                                                           acGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTtat                                                           >  2:1324235/1‑149 (MQ=255)
                                                                                           acGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTtat                                                           <  1:1324235/149‑1 (MQ=255)
                                                                                           acGCTTCAGGCTCGTAGCTCAACTGCATTAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCTGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTTGTTATCCTGGTACATTGCCAACAGGTAGTTGTtat                                                           >  3:465556/1‑149 (MQ=255)
                                                                                               ttCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCt                                                                                      <  2:699747/118‑1 (MQ=255)
                                                                                                 cAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCATATCCGACTGACAAGTCGGATAAGGCTCAAGGAAAt                                                                                                                             <  3:411256/77‑1 (MQ=255)
                                                                                                  aGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                          <  4:1348160‑M2/63‑11 (MQ=255)
                                                                                                  aGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAagtcggataa                                                                                                                                          >  3:1348160‑M2/1‑53 (MQ=255)
                                                                                                      tCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGcgccgc                                                                                                          >  4:780246/1‑91 (MQ=255)
                                                                                                        gTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCaa                                                                                                                                   >  1:674290/1‑64 (MQ=255)
                                                                                                           gCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACata                                                                                                                  >  2:621332/1‑78 (MQ=255)
                                                                                                           gCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACata                                                                                                                  <  1:621332/78‑1 (MQ=255)
                                                                                                             tCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                         >  3:540598/1‑117 (MQ=255)
                                                                                                                aCTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAtt                                                                                                      <  1:103712/85‑1 (MQ=255)
                                                                                                                aCTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAtt                                                                                                      >  2:103712/1‑85 (MQ=255)
                                                                                                                    ccTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGcc                                  >  3:1089230/1‑149 (MQ=255)
                                                                                                                       gagaCAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACa                                                                                                                    >  1:1310148/1‑64 (MQ=255)
                                                                                                                           cAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAActct                                                     >  1:1415015/1‑123 (MQ=255)
                                                                                                                           cAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAActct                                                     <  2:1415015/123‑1 (MQ=255)
                                                                                                                            aaaGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGtt                                                                                           <  2:87479/84‑1 (MQ=255)
                                                                                                                               gTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgtca                                                            >  4:109974/1‑110 (MQ=255)
                                                                                                                               gTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTta                                                            <  3:109974/112‑1 (MQ=255)
                                                                                                                                tAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTg                                             <  1:796842/126‑1 (MQ=255)
                                                                                                                                  aaaaGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAAc                                                        <  4:940142/113‑1 (MQ=255)
                                                                                                                                  aaaaGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAAc                                                        >  3:940142/1‑113 (MQ=255)
                                                                                                                                   aaaGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCa                             <  3:719344/139‑1 (MQ=255)
                                                                                                                                       ccTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTgg                                                                                             <  3:666666/71‑1 (MQ=255)
                                                                                                                                       ccTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAg                                                                      <  4:749636/94‑1 (MQ=255)
                                                                                                                                       ccTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTACAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatcatc               >  2:1311824/1‑149 (MQ=255)
                                                                                                                                        cTTATCCGACTTGTCAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACa                                                                       >  2:1205461/1‑92 (MQ=38)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgtt                                                                >  3:1269219/1‑99 (MQ=255)
                                                                                                                                        cTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatc                  <  4:1250747/145‑1 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaa                            <  2:30144‑M2/134‑121 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataagg                                                 <  4:1441845‑M2/113‑100 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgt                                                                                         <  4:297398‑M2/73‑60 (MQ=255)
                                                                                                                                         ttATCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgt                                                                                         >  3:297398‑M2/1‑14 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgt                                                              <  3:752227/100‑1 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgt                                                              >  2:308618/1‑100 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAgttgt                                                              <  1:308618/100‑1 (MQ=255)
                                                                                                                                         ttATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAg                            <  4:1384548/134‑1 (MQ=255)
                                                                                                                                          tATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAg                                                                      >  2:1183823/1‑91 (MQ=255)
                                                                                                                                          tATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAg                                                                      <  1:1183823/91‑1 (MQ=255)
                                                                                                                                           aTCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatca                 <  4:80534/143‑1 (MQ=255)
                                                                                                                                            tCCGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaaccacgcagcgttacctga          <  2:938289‑M2/149‑139 (MQ=255)
                                                                                                                                            tCCGACTGACAagtcggataaggctttttactttgtctcaggcagt                                                                                                                 <  3:782954‑M2/46‑36 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCaa                                                                                                                         <  4:55746/38‑1 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAg                                                                  <  4:855705/93‑1 (MQ=255)
                                                                                                                                            tCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAg                                                                  >  3:855705/1‑93 (MQ=255)
                                                                                                                                             ccGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgtttcatcatgcctggcgggtaggtcggataaggcgttcacgc                                        <  2:173354‑M2/118‑109 (MQ=255)
                                                                                                                                             ccGACTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgtttcatcatgcctggcgggtaggtcggataaggcgttcacgc                                        >  1:173354‑M2/1‑10 (MQ=255)
                                                                                                                                               gACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatcatc               >  4:275056/1‑141 (MQ=255)
                                                                                                                                               gACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAatcatc               <  3:275056/141‑1 (MQ=255)
                                                                                                                                                 cTGACAagtcggataaggctttttactttgtctcaggcagttgagctacgagcctgaagcgttgttggtgcgttttatcatgcctggcgggtaggtcggataaggcgttcacgccgcatccgacaaccacgcagcgttacctgatgtga     >  1:938289‑M2/1‑6 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGaa                                                                         <  1:875688/80‑1 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCtt                               <  4:757930/122‑1 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCtt                               >  3:757930/1‑122 (MQ=255)
                                                                                                                                                  tGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGt    >  3:1408671/1‑149 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAGATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCttt                                              <  3:1005236/105‑1 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAGATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTGCCCttt                                              >  4:1005236/1‑105 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTgg                                                                                             <  3:980215/58‑1 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCtgt          <  2:1101646/141‑1 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTCa  >  1:2981/1‑149 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTCa  >  1:972775/1‑149 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTCa  >  3:890658/1‑149 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTCa  >  2:1285319/1‑149 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGATTATCCTGGTACATTGc                                                                            >  4:1226682/1‑75 (MQ=255)
                                                                                                                                                     aCAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGATTATCCTGGTACATTGc                                                                            <  3:1226682/75‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACCGTTGGTTATCCTGGTACATTGCGAACAGGTAGTTGTTATAACTCTTCCCTTTGGTCGAGTAGCCCTTCAGCTTGTGAATCATCGCTGTGGCAGTCA  >  NC_000913/3736175‑3736471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: