Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,754,875 T→C 5.4% intergenic (‑447/+98) yiaW ← / ← aldB DUF3302 family inner membrane protein/aldehyde dehydrogenase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,754,8750TC 5.4% 815.0 / 20.7 234intergenic (‑447/+98)yiaW/aldBDUF3302 family inner membrane protein/aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base T (112/109);  new base C (7/6);  total (119/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CCCGCTCACCATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTG  >  NC_000913/3754727‑3755021
                                                                                                                                                    |                                                                                                                                                  
    cTCACCATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGc                                                                                                                                                <  4:178970/149‑1 (MQ=255)
    cTCACCATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGc                                                                                                                                                <  2:161924/149‑1 (MQ=255)
      cACCATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCt                                                                                                                                              >  4:411139/1‑149 (MQ=255)
       aCCATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCtt                                                                                                                                             >  1:571635/1‑149 (MQ=255)
         cATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTAt                                                                                                                                           <  3:769797/149‑1 (MQ=255)
              aaaCGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGc                                                                                                                                      <  1:511127/149‑1 (MQ=255)
                 cgcgAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCgg                                                                                                                                       >  4:63007/1‑145 (MQ=255)
                    gagaTTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTa                                                                                                                                            >  2:650755/1‑137 (MQ=255)
                    gagaTTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACgg                                                                                                                                >  3:625169/1‑149 (MQ=255)
                      gaTTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTa                                                                                                                                                   <  3:636958/128‑1 (MQ=255)
                       aTTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGcc                                                                                                                                               >  2:762198/1‑131 (MQ=255)
                              tCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGc                                                                                                                                                <  1:207847/123‑1 (MQ=255)
                              tCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATAAGGCGTACACGccgcatc                                                                                                                                          >  4:760235/1‑124 (MQ=255)
                              tCCTGACAAAAAACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTATCAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCCTATCCGGCCTACGGTCAGGTTccc                                                                                                                      >  3:963800/1‑149 (MQ=255)
                                 tGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCgtgt                                                                                                                                                    >  4:533713/1‑116 (MQ=255)
                                 tGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTc                                                                                                                              <  1:302677/138‑1 (MQ=255)
                                        acacGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGa                                                                                                            <  3:411139/149‑1 (MQ=255)
                                          acGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGTCCTACGGTCAGGTTCCCGTAGGCATGATa                                                                                                          >  4:906102/1‑149 (MQ=255)
                                          acGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATg                                                                                                             >  3:99024/1‑146 (MQ=255)
                                              cccAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGc                                                                                                                                      <  2:363189/117‑1 (MQ=255)
                                              cccAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      >  1:820600/1‑149 (MQ=255)
                                               ccAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTccc                                                                                                                      <  2:903734/132‑1 (MQ=255)
                                                cAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGc                                                                                                                                      >  4:766283/1‑115 (MQ=255)
                                                 aGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATcc                                                                                                                                         <  3:1225880/111‑1 (MQ=255)
                                                 aGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAg                                                                                                                  >  1:892583/1‑134 (MQ=255)
                                                 aGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAcgcg                                                                                                   <  4:963800/149‑1 (MQ=255)
                                                    aaCGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATcc                                                                                                                                         >  3:1389207/1‑108 (MQ=255)
                                                      cGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGc                                                                                              <  4:663294/149‑1 (MQ=255)
                                                           ttCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCt                                                                                                                                              >  4:114506/1‑96 (MQ=255)
                                                               gTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCt                                                                                                                                              <  2:1133567/92‑1 (MQ=255)
                                                                   gCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCa                                                                                 >  2:1241891/1‑149 (MQ=255)
                                                                    cGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCaa                                                                                <  3:700566/149‑1 (MQ=255)
                                                                         tCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCa                                                                                                               <  1:152414/113‑1 (MQ=255)
                                                                           tGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAGCAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                           <  3:224999/131‑1 (MQ=255)
                                                                              gTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAcgc                                                                                                    <  4:1377618/119‑1 (MQ=255)
                                                                                  ttGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                           >  4:341487/1‑124 (MQ=255)
                                                                                      gcACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                           >  1:787067/1‑120 (MQ=255)
                                                                                      gcACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                           <  2:787067/120‑1 (MQ=255)
                                                                                       cACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCa                                                             <  4:433465/149‑1 (MQ=255)
                                                                                        aCGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAgg                                                                                   <  2:221303/126‑1 (MQ=255)
                                                                                          gTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGc                                                                                                                                      <  1:1201880/73‑1 (MQ=255)
                                                                                          gTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGa                                                                                                       <  2:276073/104‑1 (MQ=255)
                                                                                          gTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCACTGCGAc                                                               <  2:1219237/144‑1 (MQ=255)
                                                                                          gTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGAc                                                               >  1:1219237/1‑144 (MQ=255)
                                                                                            aGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGAt                                                                                                           >  4:1075432/1‑98 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTACCAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAgg                                                                                                                           <  2:397738/80‑1 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCAt                                                                                                              <  2:95344/93‑1 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATaa                                                                                                         <  3:320085/98‑1 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATaa                                                                                                         >  4:320085/1‑98 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      >  1:1145001/1‑101 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      <  2:1145001/101‑1 (MQ=255)
                                                                                              gTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGcttctt                                                      <  2:820600/149‑1 (MQ=255)
                                                                                               tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATaa                                                                                                         >  3:1098900/1‑97 (MQ=255)
                                                                                               tttAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGc                                                                                              <  1:1445889/108‑1 (MQ=255)
                                                                                                  aaaaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTatat                                                  >  1:387079/1‑149 (MQ=255)
                                                                                                   aaaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                           >  3:614940/1‑107 (MQ=255)
                                                                                                    aaCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCa                                                >  3:1142265/1‑149 (MQ=255)
                                                                                                     aCGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCTTACGCGTc                                                                                           <  2:864139/105‑1 (MQ=255)
                                                                                                       gTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGaa                                             <  4:1120125/149‑1 (MQ=255)
                                                                                                        tAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAg                                                                                                                  >  4:1060370/1‑79 (MQ=255)
                                                                                                        tAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAg                                                                                                                  <  3:1060370/79‑1 (MQ=255)
                                                                                                        tAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCaa                                                                     >  3:187072/1‑124 (MQ=255)
                                                                                                        tAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAAc                                            >  1:62776/1‑149 (MQ=255)
                                                                                                         aaaTTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATa                                                                          <  2:338851/118‑1 (MQ=255)
                                                                                                          aaTTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      >  2:462619/1‑89 (MQ=255)
                                                                                                          aaTTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      <  1:462619/89‑1 (MQ=255)
                                                                                                           aTTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      >  3:764190/1‑88 (MQ=255)
                                                                                                            ttGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAg                                                                                                                  >  3:439604/1‑75 (MQ=255)
                                                                                                               tAACAACGTGCGCTTTGTTTATGTCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGTaa                                                                                <  2:1418384/106‑1 (MQ=255)
                                                                                                               tAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTc                                                                                                                              >  3:1457383/1‑60 (MQ=255)
                                                                                                                 acaacGTGCGCTTTGTTTATGCCGGATGCGGTGTGTACGCCTTATc                                                                                                                                          >  1:942660/1‑46 (MQ=255)
                                                                                                                  caacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCTTCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGaa                                             >  2:57819/1‑138 (MQ=255)
                                                                                                                  caacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACg                                  <  2:62776/149‑1 (MQ=255)
                                                                                                                   aacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCg                                                                 <  3:405478/117‑1 (MQ=255)
                                                                                                                   aacGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGccc                                       >  4:256934/1‑143 (MQ=255)
                                                                                                                      gTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATAccc                                                                       <  2:1315610/108‑1 (MQ=255)
                                                                                                                        gcgcTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTAt                            <  1:645515/149‑1 (MQ=255)
                                                                                                                         cgcTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATc                           <  2:496562/149‑1 (MQ=255)
                                                                                                                           cTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGtt                                                                                                                         <  2:1003351/53‑1 (MQ=255)
                                                                                                                           cTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGtt                                                                                                                         >  1:1003351/1‑53 (MQ=255)
                                                                                                                           cTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      <  4:1041986/72‑1 (MQ=255)
                                                                                                                           cTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGAc                                                                                                      >  3:1041986/1‑72 (MQ=255)
                                                                                                                            tttGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCTTACGCGTc                                                                                           <  4:1439154/82‑1 (MQ=255)
                                                                                                                               gTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGc                                                           <  4:607004/111‑1 (MQ=255)
                                                                                                                                tttATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATg                                                                                                             <  3:341471/60‑1 (MQ=255)
                                                                                                                                tttATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAg                    >  1:196221/1‑149 (MQ=255)
                                                                                                                                 ttATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGc                                                                                              <  1:1024635/74‑1 (MQ=255)
                                                                                                                                 ttATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGc                                                                                              >  2:1024635/1‑74 (MQ=255)
                                                                                                                                     gCCGGATAAGGCGTACACGCCGCATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATg                                                                   >  3:827430/1‑97 (MQ=25)
                                                                                                                                     gCCGGATAAGGCGTACACGCCGCATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTta                                                     <  1:447676/111‑1 (MQ=37)
                                                                                                                                      ccGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGtt                               >  1:717595/1‑132 (MQ=255)
                                                                                                                                        ggATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAG            >  3:5169‑M1/1‑149 (MQ=255)
                                                                                                                                          ataaggcgTACACGCCGCATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCa                                                <  4:1465217/107‑1 (MQ=38)
                                                                                                                                          ataaggcgTACACGCCGCATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGa                        >  1:353363/5‑135 (MQ=39)
                                                                                                                                           tGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGGCGCGTAAGCGTCGCATCCGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACcagcag         >  1:1373138/1‑149 (MQ=255)
                                                                                                                                            gcggcgTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAAt                                                                    >  2:199496/1‑89 (MQ=255)
                                                                                                                                              ggcgTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCa                                                                                     <  1:230912/70‑1 (MQ=255)
                                                                                                                                               gcgTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTc                                                                                           >  1:1119249/1‑63 (MQ=255)
                                                                                                                                                    tACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGcttctt                                                      >  2:1158210/1‑95 (MQ=255)
                                                                                                                                                    tACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGcttctt                                                      <  1:1158210/95‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCGCTCACCATCGAAACGCGAGATTAAACTCCTGACAAAACACGCCCCAGAAACGTACTTCTGTTGGCGTAGTCTGGGTTATTGCGCACGTAGGTTTAAAACGTAAATTGTAACAACGTGCGCTTTGTTTATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTG  >  NC_000913/3754727‑3755021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: