Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,778,879 T→C 9.2% V494A (GTC→GCC)  lldP → L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,778,8790TC 9.2% 2395.6 / 159.4 748V494A (GTC→GCC) lldPL‑lactate permease
Reads supporting (aligned to +/- strand):  ref base T (332/347);  new base C (38/31);  total (370/378)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 757 total aligned reads displayed.

TTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAGGCT  >  NC_000913/3778731‑3779027
                                                                                                                                                    |                                                                                                                                                    
ttctCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGt                                                                                                                                                      >  3:110592/1‑149 (MQ=255)
   tcGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTTGTTGCCGCCAATACCACCGGTGGCGTCAc                                                                                                                                                   >  3:380289/1‑149 (MQ=255)
   tcGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCAc                                                                                                                                                   >  1:297561/1‑149 (MQ=255)
       cGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGt                                                                                                                                               >  1:654983/1‑149 (MQ=255)
       cGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGt                                                                                                                                               >  4:1183017/1‑149 (MQ=255)
       cGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGt                                                                                                                                               >  1:223355/1‑149 (MQ=255)
        gTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTa                                                                                                                                              <  3:1183017/149‑1 (MQ=255)
        gTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGtg                                                                                                                                              <  2:511945/149‑2 (MQ=255)
           ccTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAga                                                                                                                                           <  2:1322260/149‑1 (MQ=255)
            cTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgat                                                                                                                                          <  4:865699/149‑1 (MQ=255)
             tCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatg                                                                                                                                         >  1:142004/1‑149 (MQ=255)
               ggctggctGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatgat                                                                                                                                       >  4:742770/1‑149 (MQ=255)
               ggctggctGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAgatgat                                                                                                                                       <  3:240410/149‑1 (MQ=255)
                       gggggTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGc                                                                                                                               <  2:297561/149‑1 (MQ=255)
                           gTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTaa                                                                                                                                             <  2:1007110/131‑1 (MQ=255)
                            tATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCAc                                                                                                                                                   >  1:886582/1‑124 (MQ=255)
                             aTTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATcta                                                                                                                         <  3:1311253/149‑1 (MQ=255)
                               tCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatc                                                                                                                       <  4:31626/149‑1 (MQ=255)
                                  tGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatcgct                                                                                                                    >  2:565166/1‑149 (MQ=255)
                                     ccGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatcgctatc                                                                                                                 <  4:110592/149‑1 (MQ=255)
                                            ggATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCtgtg                                                                                                          >  2:1140911/1‑149 (MQ=255)
                                             gATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTgc                                                                                                         >  2:809980/1‑149 (MQ=255)
                                                 ccTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGAtct                                                                                                                                     >  2:387550/1‑117 (MQ=255)
                                                  cTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGACGCCACCGGtg                                                                                                                                              <  4:640868/107‑2 (MQ=255)
                                                     aTCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAg                                                                                                 >  4:803111/1‑149 (MQ=255)
                                                         aaCGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCt                                                                                             >  4:102656/1‑149 (MQ=255)
                                                          aCGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtg                                                                                            >  2:1355632/1‑149 (MQ=255)
                                                            gCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAAt                                                                                                                            >  3:1335505/1‑115 (MQ=255)
                                                            gCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAAt                                                                                                                            >  2:967146/1‑115 (MQ=255)
                                                            gCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctat                                                                                                                        >  4:1375998/1‑119 (MQ=255)
                                                            gCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtggt                                                                                          <  2:142004/149‑1 (MQ=255)
                                                              ccTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCtatcgctatcgc                                                                                                               >  3:685346/1‑126 (MQ=255)
                                                              ccTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtggtgg                                                                                        <  1:601213/149‑1 (MQ=255)
                                                               cTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTccc                                                                                                                                  >  4:475353/1‑106 (MQ=255)
                                                                     gccgcGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAaga                                                                                 <  1:118481/149‑1 (MQ=255)
                                                                     gccgcGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAaga                                                                                 <  3:1281234/149‑1 (MQ=255)
                                                                      ccgcGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAagag                                                                                >  3:794868/1‑149 (MQ=255)
                                                                        gcgcTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTc                                                                              <  1:685694/149‑1 (MQ=255)
                                                                         cgcTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCt                                                                             >  1:384878/1‑149 (MQ=255)
                                                                            tGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctatcgctat                                                                                                                  >  2:224700/1‑109 (MQ=255)
                                                                            tGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAAACTATCGCTATCGCCTGTgcggc                                                                                                      >  2:1200374/1‑121 (MQ=255)
                                                                                  ccACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAagag                                                                                >  2:766273/1‑137 (MQ=255)
                                                                                     ccGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATctat                                                                                                                        <  4:1302690/94‑1 (MQ=255)
                                                                                       gcagcaCAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCtt                                                               >  3:182242/1‑149 (MQ=255)
                                                                                         agcaCAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTa                                                             <  4:1466956/149‑1 (MQ=255)
                                                                                             caacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCg                                                                  <  1:84749/140‑1 (MQ=255)
                                                                                             caacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCg                                                                  >  2:84749/1‑140 (MQ=255)
                                                                                             caacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGt                                                         >  3:724083/1‑149 (MQ=255)
                                                                                              aacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtg                                                                                            >  3:614220/1‑113 (MQ=255)
                                                                                              aacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGAt                                                                          <  1:405636/131‑1 (MQ=255)
                                                                                              aacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCt                                                                <  4:20472/141‑1 (MQ=255)
                                                                                              aacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTc                                                        >  2:155373/1‑149 (MQ=255)
                                                                                              aacaaATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTc                                                        >  1:1305516/1‑149 (MQ=255)
                                                                                                   aTTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAaca                                                   >  1:1001752/1‑149 (MQ=255)
                                                                                                    ttGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTc                                                        >  4:1424675/1‑143 (MQ=255)
                                                                                                       gCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGc                                               <  2:613757/149‑1 (MQ=255)
                                                                                                         gTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCt                                             >  3:1055459/1‑149 (MQ=255)
                                                                                                            tctGATGTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                          >  4:702394/1‑149 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTaa                                                                                                                                             >  3:971855/1‑50 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATTGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAGACACAGCCTGAt                                          <  2:1045295/149‑1 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGa                                           <  3:1456610/148‑1 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGa                                           >  4:1270938/1‑148 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                          <  3:1216583/149‑1 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                          >  1:95761/1‑149 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                          <  2:415525/149‑1 (MQ=255)
                                                                                                            tctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                          >  3:518035/1‑149 (MQ=255)
                                                                                                             ctGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATc                                         <  3:142111/149‑1 (MQ=255)
                                                                                                              tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTg                                                                       >  3:442722/1‑118 (MQ=255)
                                                                                                              tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGt                                                                      >  4:35692/1‑119 (MQ=255)
                                                                                                              tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGAt                                          <  3:881980/147‑1 (MQ=255)
                                                                                                              tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATc                                         <  1:465475/148‑1 (MQ=255)
                                                                                                              tGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCt                                        >  2:463030/1‑149 (MQ=255)
                                                                                                               gATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGAttt                                                                        >  4:538221/1‑116 (MQ=255)
                                                                                                                aTCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTg                                                          >  2:1261380/1‑129 (MQ=255)
                                                                                                                 tCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTc                                                                              >  1:1248587/1‑108 (MQ=255)
                                                                                                                 tCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGtt                                                                     >  4:951046/1‑117 (MQ=255)
                                                                                                                 tCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCa                                     >  1:747213/1‑149 (MQ=255)
                                                                                                                  cTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCATACACAGCCTGATc                                         <  2:715985/144‑1 (MQ=255)
                                                                                                                  cTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCAc                                    <  1:498806/149‑1 (MQ=255)
                                                                                                                    gTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGTCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCtt                                       <  4:1064626/144‑1 (MQ=255)
                                                                                                                    gTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCt                                  <  2:1075722/149‑1 (MQ=255)
                                                                                                                       gCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGta                               <  1:563891/149‑1 (MQ=255)
                                                                                                                          ggTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCtggt                                                                                          <  1:349708/87‑1 (MQ=255)
                                                                                                                          ggTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAg                            <  2:351714/149‑1 (MQ=255)
                                                                                                                           gTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGt                           <  1:1362044/149‑1 (MQ=255)
                                                                                                                           gTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGt                           >  4:572408/1‑149 (MQ=255)
                                                                                                                            ttGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTg                          <  4:279477/149‑1 (MQ=255)
                                                                                                                                cgccAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCtt                                       >  2:667468/1‑132 (MQ=255)
                                                                                                                                 gccAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGt                     >  3:845870/1‑149 (MQ=255)
                                                                                                                                   cAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCt                                                                                             <  1:787431/75‑1 (MQ=255)
                                                                                                                                     atcttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGa                                           >  3:1026218/6‑123 (MQ=255)
                                                                                                                                       cttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGta                               <  3:294974/130‑1 (MQ=255)
                                                                                                                                       cttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATca                >  1:1071762/4‑148 (MQ=255)
                                                                                                                                        ttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGc                                                                 >  1:1037273/3‑98 (MQ=255)
                                                                                                                                        ttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATcacc              <  3:329945/147‑1 (MQ=255)
                                                                                                                                        ttaccGGTGACGCCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATcacc              >  1:1126167/3‑149 (MQ=255)
                                                                                                                                         caccGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGta                               >  2:891824/1‑131 (MQ=255)
                                                                                                                                                 gCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAg     <  4:420672/149‑1 (MQ=255)
                                                                                                                                                  cGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAgg    <  3:544966/149‑1 (MQ=255)
                                                                                                                                                   gTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAGGc   >  1:155091/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTCTCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGCCTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTATAGTGGGCGTGATCACCACGCTTCAGGCT  >  NC_000913/3778731‑3779027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: