Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,897,614 C→G 7.5% L37V (CTG→GTG) ‡ cbrB → PRK09823 family inner membrane protein, creBC regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,6140CG 7.5% 615.9 / 30.6 174L37V (CTG→GTG) ‡cbrBPRK09823 family inner membrane protein, creBC regulon
Reads supporting (aligned to +/- strand):  ref base C (77/84);  new base G (6/7);  total (83/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 178 total aligned reads displayed.

GCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGT  >  NC_000913/3897466‑3897757
                                                                                                                                                     |                                                                                                                                               
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACGTATTCTTCGCAAAAGAACCGc                                                                                                                                                 <  4:1027369/149‑1 (MQ=255)
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGc                                                                                                                                                 <  3:752877/149‑1 (MQ=255)
 cTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                <  2:1064978/149‑1 (MQ=255)
      aTTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTc                                                                                                                                           <  1:166165/149‑1 (MQ=255)
         ggCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCttt                                                                                                                                        >  2:338512/1‑149 (MQ=255)
         ggCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCttt                                                                                                                                        <  3:351371/149‑1 (MQ=255)
          gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                       <  3:1105396/149‑2 (MQ=255)
          gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                       <  2:1342572/149‑2 (MQ=255)
          gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                       >  2:533750/1‑149 (MQ=255)
          gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                       <  2:1424733/149‑2 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                       <  1:155295/136‑2 (MQ=255)
                        gtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGata                                                                                                                                <  2:267875/142‑1 (MQ=255)
                           aaGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTAAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAATAGAACCGCTGGTTCTTTGGGTGATAATACATCCaa                                                                                                                      >  3:496292/1‑149 (MQ=255)
                               gAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCCAAAGAACCAGCGGTTCttt                                                                                                                                        <  4:678398/127‑1 (MQ=255)
                                  tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCttttg                                                                                                                                      <  2:62319/126‑3 (MQ=255)
                                  tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                               >  2:1375883/1‑117 (MQ=255)
                                  tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                               <  1:1375883/117‑1 (MQ=255)
                                  tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCttt                                                                                                                                        <  1:318667/124‑1 (MQ=255)
                                  tttGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCttttg                                                                                                                                      >  1:62319/1‑124 (MQ=255)
                                      atatGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                       <  3:592210/121‑1 (MQ=255)
                                      atatGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTa                                                                                                           <  4:1102028/149‑1 (MQ=255)
                                        atGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTAtt                                                                                                         <  1:338512/149‑1 (MQ=255)
                                          gTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAAtt                                                                                                                    >  4:128272/1‑136 (MQ=255)
                                          gTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAAtt                                                                                                                    <  3:128272/136‑1 (MQ=255)
                                          gTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGt                                                                                                       <  1:1358273/149‑1 (MQ=255)
                                           tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCttt                                                                                                                                        <  4:451826/115‑1 (MQ=255)
                                           tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                      >  3:895425/1‑149 (MQ=255)
                                           tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                      >  2:1305135/1‑149 (MQ=255)
                                             tGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTgg                                                                                                                                      <  3:437301/115‑1 (MQ=255)
                                               tttCACGTCGGGTAATACATCACGGACTTTATTTTGCCGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                    <  2:834492/115‑1 (MQ=255)
                                               tttCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                    >  1:834492/1‑115 (MQ=255)
                                                     gTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCaa                                                                                                                      >  3:238338/1‑123 (MQ=255)
                                                     gTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCaa                                                                                                                      <  4:238338/123‑1 (MQ=255)
                                                      tCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACgg                                                                                           <  2:1443775/149‑1 (MQ=255)
                                                           tAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCt                                                                                      >  3:999937/1‑149 (MQ=255)
                                                             aTACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTa                                                                                              >  2:437154/1‑139 (MQ=255)
                                                             aTACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTa                                                                                              <  1:437154/139‑1 (MQ=255)
                                                              tACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGa                                                                                                    <  4:856120/132‑1 (MQ=255)
                                                              tACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGa                                                                                                    >  3:856120/1‑132 (MQ=255)
                                                                cATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                       >  3:1160798/1‑94 (MQ=255)
                                                                cATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTGCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCtttt                                                                                                                                       <  4:1160798/95‑2 (MQ=255)
                                                                    aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTgg                                                                                                                                              <  3:1030954/84‑1 (MQ=255)
                                                                    aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATaa                                                                                                 <  1:933388/129‑1 (MQ=255)
                                                                    aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCg                                                                             >  2:601579/1‑149 (MQ=255)
                                                                     cGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgt                                                                            <  2:15935/149‑1 (MQ=255)
                                                                      ggACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                           >  4:726380/1‑149 (MQ=255)
                                                                           ttATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTaa                                                                      <  1:568385/149‑1 (MQ=255)
                                                                                  gCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                       >  4:325215/1‑77 (MQ=255)
                                                                                     gTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTATTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAAt                                                            <  4:122368/149‑1 (MQ=255)
                                                                                      ttttAGGACCGTAAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                           >  3:104681/1‑133 (MQ=255)
                                                                                        gtAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGt                                                         <  4:582573/148‑1 (MQ=255)
                                                                                          aGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTg                                                       <  2:328695/149‑1 (MQ=255)
                                                                                                    aTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGcc                                             >  3:1256726/1‑149 (MQ=255)
                                                                                                    aTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCCGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGcc                                             >  4:953376/1‑149 (MQ=255)
                                                                                                    aTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATACCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGcc                                             >  2:92961/1‑149 (MQ=255)
                                                                                                    aTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCGATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGcc                                             <  3:953376/149‑1 (MQ=255)
                                                                                                          gtTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATACTACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGaaaa                                       >  3:852514/1‑149 (MQ=255)
                                                                                                           ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                               <  2:49631/44‑1 (MQ=255)
                                                                                                           ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                               >  1:49631/1‑44 (MQ=255)
                                                                                                           ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttgtt                                                                        >  4:1033179/1‑115 (MQ=255)
                                                                                                           ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttg                                                                          <  1:759756/113‑1 (MQ=255)
                                                                                                           ttCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttg                                                                          >  2:759756/1‑113 (MQ=255)
                                                                                                             cggTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAAAACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGAt                                    <  4:1256726/147‑1 (MQ=255)
                                                                                                                tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGtt                                                        <  3:1081219/126‑1 (MQ=255)
                                                                                                                tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGtt                                                        >  4:1081219/1‑126 (MQ=255)
                                                                                                                tttCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                 >  3:23411/1‑149 (MQ=255)
                                                                                                                       tCCTATACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGa                                                                                         <  1:1059854/86‑1 (MQ=255)
                                                                                                                         ctctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTggg                                                                                                                                     >  4:197512/1‑40 (MQ=255)
                                                                                                                         ctctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTggg                                                                                                                                     <  3:197512/40‑1 (MQ=255)
                                                                                                                          tctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGaaa                       <  4:496292/149‑1 (MQ=255)
                                                                                                                           ctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgt                                                                            >  4:871360/1‑95 (MQ=255)
                                                                                                                           ctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAAc                      <  3:868931/149‑1 (MQ=255)
                                                                                                                                 atTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCgg                                                                  <  1:1289484/99‑1 (MQ=255)
                                                                                                                                 atTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCgg                                                                  >  2:1289484/1‑99 (MQ=255)
                                                                                                                                 atTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATc                >  3:1034391/1‑149 (MQ=255)
                                                                                                                                 atTCTTCGCAAAAGAACCGCTGGTTACTTCGGTGATCATACATCCAATTTTTCTCTTATAGTCCATAACTACGGCAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTCTCTGCCGGAACAGATCGTGTCACAGAAACGTTATc                <  4:907079/149‑1 (MQ=255)
                                                                                                                                  ttcttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                 <  1:976299/131‑1 (MQ=255)
                                                                                                                                  ttcttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCgg                                 >  2:976299/1‑131 (MQ=255)
                                                                                                                                   tcttcGCAAAAGGACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTaat                                                                     <  1:1308252/94‑2 (MQ=255)
                                                                                                                                     ttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAAc                                                                     <  1:393894/92‑1 (MQ=255)
                                                                                                                                     ttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAAc                                                                     >  2:393894/1‑92 (MQ=255)
                                                                                                                                      tcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGt                               >  1:103030/1‑129 (MQ=255)
                                                                                                                                       acccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgtt                                                                           <  2:1040739/80‑1 (MQ=38)
                                                                                                                                        gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGAt                                    >  4:86783/1‑122 (MQ=255)
                                                                                                                                        gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTct         >  1:609779/1‑149 (MQ=255)
                                                                                                                                          aaaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCggg                                <  4:1311894/124‑1 (MQ=255)
                                                                                                                                          aaaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCggg                                >  3:1311894/1‑124 (MQ=255)
                                                                                                                                          aaaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAAc                      >  3:721768/1‑134 (MQ=255)
                                                                                                                                          aaaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGt           <  3:548868/145‑1 (MQ=255)
                                                                                                                                          aaaaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctgg       >  1:717902/1‑149 (MQ=255)
                                                                                                                                           aaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGc                                                      <  2:1134315/101‑1 (MQ=255)
                                                                                                                                           aaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGc                                                      >  1:1134315/1‑101 (MQ=255)
                                                                                                                                            aaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGtt                                                        <  1:1424442/98‑1 (MQ=255)
                                                                                                                                            aaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGtt                                                        >  2:1424442/1‑98 (MQ=255)
                                                                                                                                            aaGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTcac                            <  4:1045478/126‑1 (MQ=255)
                                                                                                                                               aaCCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGt           >  4:363925/1‑140 (MQ=255)
                                                                                                                                               aaCCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCtggt  <  4:23411/149‑1 (MQ=255)
                                                                                                                                                    gCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTaa                                                             <  1:176461/86‑1 (MQ=255)
                                                                                                                                                     cTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc      <  3:1322281/140‑1 (MQ=255)
                                                                                                                                                     cTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc      >  4:1322281/1‑140 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGT  >  NC_000913/3897466‑3897757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: