Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,923,717 G→A 7.3% intergenic (‑37/+27) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,7170GA 7.3% 313.0 / 10.8 96intergenic (‑37/+27)rsmG/mnmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base G (42/47);  new base A (4/3);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 108 total aligned reads displayed.

CGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAA  >  NC_000913/3923570‑3923865
                                                                                                                                                   |                                                                                                                                                    
cGCTTTGTTCCATTTGTGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     <  1:1100338/149‑1 (MQ=255)
cGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     >  1:180981/1‑149 (MQ=255)
 gCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCggg                                                                                                                                                    >  2:403323/1‑149 (MQ=255)
  cTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  1:558677/149‑1 (MQ=255)
  cTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  1:403323/149‑1 (MQ=255)
  cTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  2:1065682/149‑1 (MQ=255)
  cTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  2:1077588/149‑1 (MQ=255)
     tGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCt                                                                                                                                                <  4:163125/149‑1 (MQ=255)
       ttCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     >  3:830772/1‑142 (MQ=255)
       ttCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     <  4:830772/142‑1 (MQ=255)
       ttCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTa                                                                                                                                              >  1:979690/1‑149 (MQ=255)
         ccATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTAcc                                                                                                                                            <  1:1305799/149‑1 (MQ=255)
           aTTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                          <  1:1451822/149‑1 (MQ=255)
           aTTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                          <  3:165509/149‑1 (MQ=255)
           aTTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                          >  4:165509/1‑149 (MQ=255)
               aTGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     >  1:238350/1‑134 (MQ=255)
               aTGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGAGTTGCCTGGTAAGCgg                                                                                                                                                     <  2:238350/134‑1 (MQ=255)
                  cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     <  1:883830/131‑1 (MQ=255)
                  cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                     >  2:883830/1‑131 (MQ=255)
                   agcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAGGc                                                                                                                                        >  2:861111/1‑143 (MQ=255)
                   agcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAGGc                                                                                                                                        <  1:861111/143‑1 (MQ=255)
                    gcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  2:461542/131‑1 (MQ=255)
                    gcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   >  1:461542/1‑131 (MQ=255)
                     caTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTaa                                                                                                                                <  2:979690/149‑1 (MQ=255)
                         ttCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATg                                                                                                                              <  1:1051605/147‑1 (MQ=255)
                         ttCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATg                                                                                                                              >  2:1051605/1‑147 (MQ=255)
                            aCGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   >  1:116195/1‑123 (MQ=255)
                            aCGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  2:116195/123‑1 (MQ=255)
                            aCGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGt                                                                                                                           <  3:1000890/147‑1 (MQ=255)
                            aCGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGt                                                                                                                           >  4:1000890/1‑147 (MQ=255)
                                          cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCg                                                                                                                            <  1:378515/132‑1 (MQ=255)
                                          cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCg                                                                                                                            >  2:378515/1‑132 (MQ=255)
                                          cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgc                                                                                                                      >  2:987146/1‑138 (MQ=255)
                                          cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgc                                                                                                                      <  1:987146/138‑1 (MQ=255)
                                           tGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcag                                                                                                          >  2:1292958/1‑149 (MQ=255)
                                            ggTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGc                                                                                                                             >  2:29072/1‑129 (MQ=255)
                                                  tCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAccc                                                                                                   >  3:1128207/1‑149 (MQ=255)
                                                    tGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  3:1141020/99‑1 (MQ=255)
                                                    tGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   <  2:761912/99‑1 (MQ=255)
                                                    tGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                   >  4:1141020/1‑99 (MQ=255)
                                                     ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGt                                                                                                <  4:1128207/149‑1 (MQ=255)
                                                      gTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTa                                                                                                                         >  4:1048009/1‑123 (MQ=255)
                                                       tGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTaa                                                                                                                                >  1:658926/1‑115 (MQ=255)
                                                       tGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTaa                                                                                                                                <  2:658926/115‑1 (MQ=255)
                                                        gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                          >  3:821487/1‑104 (MQ=255)
                                                         aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           >  2:188594/1‑102 (MQ=255)
                                                         aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           <  1:188594/102‑1 (MQ=255)
                                                         aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                          <  3:735287/103‑1 (MQ=255)
                                                         aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                          >  4:735287/1‑103 (MQ=255)
                                                         aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                            >  4:1163198/1‑149 (MQ=255)
                                                            ggTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCa                                                                                         <  1:1292958/149‑1 (MQ=255)
                                                                    aaaTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAg                                                                                        >  3:1322104/1‑142 (MQ=255)
                                                                    aaaTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAg                                                                                        <  4:1322104/142‑1 (MQ=255)
                                                                          cTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           <  3:1250096/85‑1 (MQ=255)
                                                                          cTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           >  4:1250096/1‑85 (MQ=255)
                                                                            gCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           >  2:241810/1‑83 (MQ=255)
                                                                            gCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           <  1:241810/83‑1 (MQ=255)
                                                                                 tttCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                   >  1:1078650/1‑86 (MQ=255)
                                                                                 tttCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                   <  2:1078650/86‑1 (MQ=255)
                                                                                         gTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATa                                                                                                      >  2:868369/1‑107 (MQ=255)
                                                                                         gTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATa                                                                                                      <  1:868369/107‑1 (MQ=255)
                                                                                            aGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATg                                                                        <  3:1244944/134‑1 (MQ=255)
                                                                                            aGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATg                                                                        >  4:1244944/1‑134 (MQ=255)
                                                                                             ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATgg                                                                 <  3:1138289/140‑1 (MQ=255)
                                                                                             ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATgg                                                                 >  4:1138289/1‑140 (MQ=255)
                                                                                              gagaGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                    >  2:708868/1‑120 (MQ=255)
                                                                                              gagaGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                    <  1:708868/120‑1 (MQ=255)
                                                                                                  gTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           >  2:512921/1‑61 (MQ=255)
                                                                                                  gTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                           <  1:512921/61‑1 (MQ=255)
                                                                                                              cGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGCCGTGACGCCAGAAATAcgc                                       <  3:1163198/149‑1 (MQ=255)
                                                                                                               ggTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtttt                                                                                             >  4:221517/1‑94 (MQ=255)
                                                                                                               ggTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtttt                                                                                             <  3:221517/94‑1 (MQ=255)
                                                                                                                 tGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGaa                                    >  1:487314/1‑149 (MQ=255)
                                                                                                                     tACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGga                                                                      <  4:1169918/111‑1 (MQ=255)
                                                                                                                     tACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGga                                                                      >  3:1169918/1‑111 (MQ=255)
                                                                                                                         tGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATTCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGaa                                                                          >  1:462164/1‑103 (MQ=255)
                                                                                                                         tGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGaa                                                                          <  2:462164/103‑1 (MQ=255)
                                                                                                                           ttCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcagca                                                                                                        <  4:218424/71‑1 (MQ=255)
                                                                                                                              ttGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTac                                                                                                                 >  3:166816/1‑59 (MQ=255)
                                                                                                                              ttGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTac                                                                                                                 <  4:166816/59‑1 (MQ=255)
                                                                                                                                     gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                            >  4:1413376/1‑137 (MQ=255)
                                                                                                                                     gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                            <  3:1413376/137‑1 (MQ=255)
                                                                                                                                        gCCTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATgg                                                                       >  3:743014/1‑91 (MQ=38)
                                                                                                                                         ccTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGc                                                  >  2:238885/1‑111 (MQ=255)
                                                                                                                                         ccTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGc                                                  <  1:238885/111‑1 (MQ=255)
                                                                                                                                          cTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                       <  2:770005/105‑1 (MQ=255)
                                                                                                                                           tGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgtg          >  2:1229300/1‑149 (MQ=255)
                                                                                                                                             gTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCGGCCACACCAGCAGAATGga                                                                      <  2:394063/87‑1 (MQ=255)
                                                                                                                                             gTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGga                                                                      >  1:394063/1‑87 (MQ=255)
                                                                                                                                              tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcgc                                       >  2:950817/1‑117 (MQ=255)
                                                                                                                                              tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcgc                                       <  1:950817/117‑1 (MQ=255)
                                                                                                                                              tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATc       <  2:487314/149‑1 (MQ=255)
                                                                                                                                              tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATc       >  2:893540/1‑149 (MQ=255)
                                                                                                                                               aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGAc                                                    <  1:569195/103‑1 (MQ=255)
                                                                                                                                               aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGAc                                                    >  2:569195/1‑103 (MQ=255)
                                                                                                                                               aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt           >  1:547653/1‑144 (MQ=255)
                                                                                                                                               aaGCGGGTGCTTACCAGGCATTTTAAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGAGACGCCAGAAATAc                                         >  3:916012/1‑114 (MQ=255)
                                                                                                                                                   gggTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt           <  4:1364681/140‑1 (MQ=255)
                                                                                                                                                   gggTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt           >  3:1364681/1‑140 (MQ=255)
                                                                                                                                                   gggTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTaa  >  1:1147186/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAA  >  NC_000913/3923570‑3923865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: