Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,980,776 T→G 7.0% intergenic (+80/‑111) wecG → / → yifK UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative APC family amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,7760TG 7.0% 356.8 / 14.3 100intergenic (+80/‑111)wecG/yifKUDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (49/44);  new base G (4/3);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 106 total aligned reads displayed.

GCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGA  >  NC_000913/3980635‑3980924
                                                                                                                                             |                                                                                                                                                    
gCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCtt                                                                                                                                               <  4:300212/149‑1 (MQ=255)
             gCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtc                                                                                                                                  >  3:448906/1‑149 (MQ=255)
               ttCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                                                                                >  3:1107710/1‑149 (MQ=255)
                                              cacCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAtt                                                                                                                                           <  1:1260710/107‑1 (MQ=255)
                                              cacCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAtt                                                                                                                                           >  2:1260710/1‑107 (MQ=255)
                                                              ttttCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATaa                                                                                 <  4:1107710/149‑1 (MQ=255)
                                                                    tctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTcc                                                                                                                               >  3:340487/1‑97 (MQ=255)
                                                                    tctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTcc                                                                                                                               <  4:340487/97‑1 (MQ=255)
                                                                     ctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGc                                                                                                           >  1:507222/1‑116 (MQ=255)
                                                                     ctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGc                                                                                                           <  2:507222/116‑1 (MQ=255)
                                                                                  gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGcc                                                                                                                                       <  2:1305196/75‑1 (MQ=255)
                                                                                  gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGcc                                                                                                                                       >  1:1305196/1‑75 (MQ=255)
                                                                                  gACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACAttt                                                                                                             <  2:673517/101‑1 (MQ=255)
                                                                                  gACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACAttt                                                                                                             >  1:673517/1‑101 (MQ=255)
                                                                                  gACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAaca                                                                        >  1:978652/1‑138 (MQ=255)
                                                                                  gACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAaca                                                                        <  2:978652/138‑1 (MQ=255)
                                                                                   aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTcc                                                                                                                               >  4:1316700/1‑82 (MQ=255)
                                                                                   aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTcc                                                                                                                               <  3:1316700/82‑1 (MQ=255)
                                                                                   aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 <  3:520391/144‑1 (MQ=255)
                                                                                   aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 >  4:520391/1‑144 (MQ=255)
                                                                                   aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 >  2:132183/1‑144 (MQ=255)
                                                                                   aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTAAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 <  1:132183/144‑1 (MQ=255)
                                                                                     ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTaa                                                                                                                                                    >  1:1359387/1‑59 (MQ=255)
                                                                                     ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTaa                                                                                                                                                    <  2:1359387/59‑1 (MQ=255)
                                                                                     ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                                                                      >  1:270590/1‑121 (MQ=255)
                                                                                     ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                                                                      <  2:270590/121‑1 (MQ=255)
                                                                                          gaccACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa                                                              >  1:1340138/4‑140 (MQ=255)
                                                                                          gaccACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa                                                              <  2:1340138/137‑1 (MQ=255)
                                                                                           gacACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAt                                                             <  4:1254333/138‑1 (MQ=255)
                                                                                           gTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATg                                                                                                >  1:1431187/1‑105 (MQ=255)
                                                                                            tCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGAt                                                                                                 <  4:938573/103‑1 (MQ=255)
                                                                                            tCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGAt                                                                                                 >  3:938573/1‑103 (MQ=255)
                                                                                            tCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGc                                                           >  3:249102/1‑141 (MQ=255)
                                                                                            tCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGc                                                           <  4:249102/141‑1 (MQ=255)
                                                                                             cACTCCGTCGTCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTaa                                                                          <  1:373067/125‑1 (MQ=255)
                                                                                             cACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTaa                                                                          >  2:373067/1‑125 (MQ=255)
                                                                                              aCTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                                                                      <  4:717132/112‑1 (MQ=255)
                                                                                              aCTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                                                                      >  3:717132/1‑112 (MQ=255)
                                                                                              aCTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGc                                                           >  1:129162/1‑139 (MQ=255)
                                                                                              aCTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGc                                                           <  2:129162/139‑1 (MQ=255)
                                                                                                tCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACAt                                                                                                               <  2:1453190/85‑1 (MQ=255)
                                                                                                tCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACAt                                                                                                               >  1:1453190/1‑85 (MQ=255)
                                                                                                 ccGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGa                                              <  2:379281/149‑1 (MQ=255)
                                                                                                             tGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                                                                                <  2:1429146/55‑1 (MQ=255)
                                                                                                             tGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                                                                                >  1:1429146/1‑55 (MQ=255)
                                                                                                             tGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCg                                                                              >  4:1085494/1‑105 (MQ=255)
                                                                                                             tGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCg                                                                              <  3:1085494/105‑1 (MQ=255)
                                                                                                              gCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCg                                                                              >  4:1264159/1‑104 (MQ=255)
                                                                                                              gCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCg                                                                              <  3:1264159/104‑1 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACCCTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGc                                   <  3:863394/145‑1 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGa                                                                                                  >  3:9604/1‑82 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGa                                                                                                  <  4:9604/82‑1 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATaa                                                                                 >  3:234108/1‑99 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATaa                                                                                 <  4:234108/99‑1 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGc                                                               >  2:1376504/1‑117 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGc                                                               <  1:1376504/117‑1 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGa                                >  4:845453/1‑148 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGa                                <  3:845453/148‑1 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGAt                               >  4:522524/1‑149 (MQ=255)
                                                                                                                ttttttATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAGGCAAACACAACACGAGGATTTATGGc                                   >  4:863394/1‑145 (MQ=255)
                                                                                                                      attaCACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAaaca                                                      >  3:609580/1‑120 (MQ=255)
                                                                                                                      attaCACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAaaca                                                      <  4:609580/120‑1 (MQ=255)
                                                                                                                      attaCACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACaaa                         <  3:522524/149‑1 (MQ=255)
                                                                                                                       ttaCACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAAc                        >  4:500672/1‑149 (MQ=255)
                                                                                                                         aaataaaGCATTAAAAATTTGAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGTAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCa                      >  4:373506/5‑149 (MQ=255)
                                                                                                                          cacaAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCag                     >  2:152373/1‑149 (MQ=255)
                                                                                                                           acaAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 <  4:77439/104‑1 (MQ=255)
                                                                                                                           acaAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 >  3:77439/1‑104 (MQ=255)
                                                                                                                           acaAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAaac                                                       >  4:866737/1‑114 (MQ=255)
                                                                                                                             aaaGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGc                  >  2:17722/1‑149 (MQ=255)
                                                                                                                              aaGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATg                                     >  4:701553/1‑129 (MQ=255)
                                                                                                                              aaGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATg                                     <  3:701553/129‑1 (MQ=255)
                                                                                                                              aaGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATa                              >  1:1199312/1‑136 (MQ=255)
                                                                                                                              aaGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATa                              <  2:1199312/136‑1 (MQ=255)
                                                                                                                              aaGCATTAAAAATTTGAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 <  3:996883/101‑1 (MQ=255)
                                                                                                                              aaGCATTAAAAATTTGAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAgg                                            <  1:278257/122‑1 (MQ=255)
                                                                                                                              aaGCATTAAAAATTTGAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAgg                                            >  2:278257/1‑122 (MQ=255)
                                                                                                                               aGCATTAAAAATTTGAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATg                                     <  1:1254180/128‑1 (MQ=255)
                                                                                                                               aGCATTAAAAATTTGAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTAGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATg                                     >  2:1254180/1‑128 (MQ=255)
                                                                                                                                gCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 <  4:456290/99‑1 (MQ=255)
                                                                                                                                gCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa                                                                 >  3:456290/1‑99 (MQ=255)
                                                                                                                                gCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTAc               >  3:1454126/1‑149 (MQ=255)
                                                                                                                                  aTTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCa                                  >  4:103535/1‑128 (MQ=255)
                                                                                                                                  aTTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCa                                  <  3:103535/128‑1 (MQ=255)
                                                                                                                                  aTTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAg             >  2:526054/1‑149 (MQ=255)
                                                                                                                                     cAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCagag                   <  2:1427400/140‑1 (MQ=255)
                                                                                                                                     cAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCagag                   >  1:514335/1‑140 (MQ=255)
                                                                                                                                     cAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCagag                   >  1:1427400/1‑140 (MQ=255)
                                                                                                                                     cAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCagag                   <  2:514335/140‑1 (MQ=255)
                                                                                                                                      aaaTTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAAc                                                                                <  1:760395/78‑1 (MQ=255)
                                                                                                                                      aaaTTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAAc                                                                                >  2:760395/1‑78 (MQ=255)
                                                                                                                                      aaaTTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa                                                              >  3:946630/1‑96 (MQ=255)
                                                                                                                                      aaaTTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa                                                              <  4:946630/96‑1 (MQ=255)
                                                                                                                                      aaaTTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTg         >  2:1168009/1‑149 (MQ=255)
                                                                                                                                         tttttAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCaga                    >  4:758208/1‑135 (MQ=255)
                                                                                                                                         tttttAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCaga                    <  3:758208/135‑1 (MQ=255)
                                                                                                                                         tttttAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGc      >  4:320654/1‑149 (MQ=255)
                                                                                                                                         tttttAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGc      >  4:885549/1‑149 (MQ=255)
                                                                                                                                            ttAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTgg   <  4:1454126/149‑1 (MQ=255)
                                                                                                                                             tAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGa  <  1:1168009/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGA  >  NC_000913/3980635‑3980924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: