| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,993,569 | C→T | 100% | P806L (CCG→CTG) | cyaA → | adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,993,569 | 0 | C | T | 97.8% | 770.4 / 2.0 | 208 | P806L (CCG→CTG) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (107/96); total (109/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 210 total aligned reads displayed.
ATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAA > NC_000913/3993421‑3993713 | atCTACATTATCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGTCAGCAAAGAGGAGCTGATACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGcc > 3:940486/1‑149 (MQ=255) tACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCa > 2:924729/1‑149 (MQ=255) aCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAg > 3:311431/1‑149 (MQ=255) cATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGt < 3:116427/149‑1 (MQ=255) tctcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCt < 4:1112849/149‑1 (MQ=255) tcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTAt > 4:782686/1‑149 (MQ=255) tcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTAt < 1:924729/149‑1 (MQ=255) tcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTAt > 1:870958/1‑149 (MQ=255) cgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGa < 3:136381/149‑1 (MQ=255) gaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGAt > 3:1296909/1‑149 (MQ=255) aGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATtgt > 2:504732/1‑149 (MQ=255) aaCCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATc < 1:117924/139‑1 (MQ=255) aaCCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATc > 2:117924/1‑139 (MQ=255) aaCCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGAtt < 1:83789/144‑1 (MQ=255) aCCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAg > 1:944611/1‑149 (MQ=255) ccGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATtgt < 1:1403674/144‑1 (MQ=255) ggTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTg > 1:433933/1‑149 (MQ=255) gTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTa > 4:798231/1‑131 (MQ=255) ttGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGAt < 1:1461975/149‑1 (MQ=255) ttGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGAt < 4:311431/149‑1 (MQ=255) ggTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTAt < 2:1274921/127‑1 (MQ=255) caccacTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACa > 2:605227/1‑149 (MQ=255) accacTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAg > 2:635044/1‑149 (MQ=255) ccacTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAgg > 2:1016935/1‑149 (MQ=255) cacTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGt > 1:396685/1‑149 (MQ=255) tGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAt < 4:1296909/149‑1 (MQ=255) tGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAt > 2:1134733/1‑149 (MQ=255) cGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGaa < 3:38032/123‑1 (MQ=255) cGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttc < 1:504732/149‑1 (MQ=255) cGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttc < 2:396685/149‑1 (MQ=255) cGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttc > 3:787350/1‑149 (MQ=255) gcaAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGc < 1:605227/149‑1 (MQ=255) aaaGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGGTGGTCGTGAACAGGTGAtt > 1:28631/1‑138 (MQ=255) aaGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATc < 3:476887/102‑1 (MQ=255) aggagCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACaaaa < 2:433933/149‑1 (MQ=255) tgCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGt > 3:434257/2‑119 (MQ=255) gTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt > 4:7923/1‑146 (MQ=255) tACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGt < 4:391633/113‑1 (MQ=255) gTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt > 1:970199/1‑142 (MQ=255) tGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGAtt > 1:1311777/1‑91 (MQ=255) gACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAgg < 1:1130465/115‑1 (MQ=255) tCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACaaaa < 1:735174/131‑1 (MQ=255) tCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACaaaa > 2:735174/1‑131 (MQ=255) tCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATtgtg < 4:662298/85‑1 (MQ=255) tCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATtgtg > 3:662298/1‑85 (MQ=255) ttCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACa < 4:364800/101‑1 (MQ=255) tACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACa > 2:843325/1‑98 (MQ=255) cTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGAt < 2:1083444/85‑1 (MQ=255) cTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTg < 4:714243/140‑1 (MQ=255) tCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt > 2:820126/1‑122 (MQ=255) tCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGa > 3:713779/1‑149 (MQ=255) gTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTaa < 2:64389/126‑1 (MQ=255) gTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATc > 1:1256989/1‑149 (MQ=255) gTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATc > 3:350118/1‑149 (MQ=255) tCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACaaaa > 3:172096/1‑114 (MQ=255) aTCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGa < 2:349534/78‑1 (MQ=255) aTCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGc < 2:969513/135‑1 (MQ=255) aTCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACg > 3:794686/1‑149 (MQ=255) tCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGa < 3:852064/149‑1 (MQ=255) tCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGa > 1:1078400/1‑149 (MQ=255) gCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt < 3:360255/114‑1 (MQ=255) gCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATa < 1:1134733/149‑1 (MQ=255) aCCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAg < 3:593819/134‑1 (MQ=255) aCCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAg > 4:593819/1‑134 (MQ=255) ccGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAcgccgc > 2:1414387/1‑149 (MQ=255) ccGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAc < 4:1183592/144‑1 (MQ=255) ccGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAc > 3:1183592/1‑144 (MQ=255) gTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGTTTGTGaa > 4:457746/1‑61 (MQ=255) ttttACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgccg > 3:753263/1‑118 (MQ=255) ttttACCTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTAt > 3:816364/1‑149 (MQ=255) ttttACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTAt < 4:1413368/149‑1 (MQ=255) cTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt < 3:526037/99‑1 (MQ=255) cTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt > 4:526037/1‑99 (MQ=255) cTACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGc > 4:74890/1‑149 (MQ=255) tACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGaa > 1:643171/1‑53 (MQ=255) tACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGt > 4:1407745/1‑74 (MQ=255) tACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGt < 3:1407745/74‑1 (MQ=255) tACGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCa > 2:169788/1‑149 (MQ=255) aCGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTa < 1:680571/143‑1 (MQ=255) aCGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTa > 2:680571/1‑143 (MQ=255) aCGGCTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCaa > 3:208978/1‑149 (MQ=255) cTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCAc < 4:625475/124‑1 (MQ=255) cTCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCAc > 3:625475/1‑124 (MQ=255) tCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTAt < 1:610948/92‑1 (MQ=255) tCAAGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAttt > 4:1020430/1‑149 (MQ=255) aaGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAttttt > 4:1253078/1‑149 (MQ=255) aaGCTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAttttt > 3:1410750/1‑149 (MQ=255) cTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGt < 3:744528/149‑1 (MQ=255) cTTCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGt < 3:1020430/149‑1 (MQ=255) ttCATCAACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtg > 2:240155/1‑149 (MQ=255) tcatcaACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtga < 3:1253078/149‑1 (MQ=255) tcaACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga > 1:72064/1‑149 (MQ=255) tcaACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga < 4:208978/149‑1 (MQ=255) tcaACTTCAACCTGCTGCAGTTCTATCAGATCGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga > 1:650303/1‑149 (MQ=255) caACTTCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGaa < 4:350118/149‑1 (MQ=255) ttCAACCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgat > 1:100557/1‑142 (MQ=255) aaCCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAtttt > 2:1316625/1‑132 (MQ=255) aCCTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAcgc > 2:33065/1‑111 (MQ=255) ccTGCTGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGa > 4:872152/1‑149 (MQ=255) ccTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGa > 2:72520/1‑149 (MQ=255) | ATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAA > NC_000913/3993421‑3993713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |