| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,088,091 | G→C | 26.3% | intergenic (+34/+16) | yiiG → / ← frvR | DUF3829 family lipoprotein/putative frv operon regulator; contains a PTS EIIA domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,088,091 | 0 | G | C | 26.3% | 33.2 / 12.3 | 19 | intergenic (+34/+16) | yiiG/frvR | DUF3829 family lipoprotein/putative frv operon regulator; contains a PTS EIIA domain |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base C (2/3); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
TAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCG > NC_000913/4087949‑4088236 | tAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATc < 2:405064/149‑1 (MQ=255) aaaGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATcc > 4:713308/1‑149 (MQ=255) cAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTtgt < 3:713308/149‑1 (MQ=255) aaGACTCTTTCCCGAGAGCATTACGCGAGTACCACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGa > 1:1198764/1‑111 (MQ=255) aaGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGa < 2:1198764/111‑1 (MQ=255) tctTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGa > 4:865488/1‑106 (MQ=255) tctTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGa < 3:865488/106‑1 (MQ=255) cgcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTc < 1:177404/115‑1 (MQ=255) cgcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTc > 2:177404/1‑115 (MQ=255) aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgcc > 4:66764/1‑149 (MQ=255) tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt < 3:66764/149‑1 (MQ=255) ccTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCt < 3:164848/99‑1 (MQ=255) ccTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCt > 4:164848/1‑99 (MQ=255) ttAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGgcgc < 2:896777/41‑1 (MQ=255) ttAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGgcgc > 1:896777/1‑41 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCata > 2:719673/1‑87 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCata < 1:719673/87‑1 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATc < 2:442921/89‑1 (MQ=255) aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATc > 1:442921/1‑89 (MQ=255) gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc < 3:1035970/86‑1 (MQ=255) gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc > 4:1035970/1‑86 (MQ=255) gcgcATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcggcggcg < 3:220674/146‑1 (MQ=255) gcgcATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcggcggcg > 4:220674/1‑146 (MQ=255) | TAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCG > NC_000913/4087949‑4088236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |