Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,093,463 A→G 6.2% S270S (AGT→AGC rhaD ← rhamnulose‑1‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,093,4630AG 6.2% 374.1 / 10.8 113S270S (AGT→AGCrhaDrhamnulose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (51/55);  new base G (4/3);  total (55/58)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

Only 100 of 117 total aligned reads displayed.

TTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCAT  >  NC_000913/4093320‑4093608
                                                                                                                                               |                                                                                                                                                 
ttATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGc                                                                                                                                              <  3:803179/149‑1 (MQ=255)
       cACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGc                                                                                                                                       >  3:655351/1‑149 (MQ=255)
       cACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGc                                                                                                                                       <  4:340929/149‑1 (MQ=255)
           gTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTaa                                                                                                                                   <  3:734305/149‑1 (MQ=255)
            tGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAggg                                                                                                                                         >  1:1004564/1‑140 (MQ=255)
            tGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTg                                                                                                                                         <  2:1004564/142‑1 (MQ=255)
            tGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAAc                                                                                                                                  >  3:477314/1‑149 (MQ=255)
            tGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAAc                                                                                                                                  >  1:983776/1‑149 (MQ=255)
                   tCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGaaa                                                                                                                           <  3:595665/149‑1 (MQ=255)
                      gCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGc                                                                                                                        >  4:237538/1‑149 (MQ=255)
                         cTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTg                                                                                                                     >  4:1098696/1‑149 (MQ=255)
                           gCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGcc                                                                                                                   <  3:237538/149‑1 (MQ=255)
                                cAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCa                                                                                                                                                   >  1:526498/1‑112 (MQ=255)
                                cAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCg                                                                                                                              <  2:822483/133‑1 (MQ=255)
                                cAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCg                                                                                                                              >  1:822483/1‑133 (MQ=255)
                                cAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGc                                                                                                                        <  4:673296/139‑1 (MQ=255)
                                cAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGc                                                                                                                        >  3:673296/1‑139 (MQ=255)
                                   ttCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTa                                                                                                           >  1:340294/1‑149 (MQ=255)
                                   ttCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTa                                                                                                           >  3:795303/1‑149 (MQ=255)
                                    tCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTAt                                                                                                          <  4:477314/149‑1 (MQ=255)
                                       ggCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCaa                                                                                                       <  1:529802/149‑1 (MQ=255)
                                       ggCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCaa                                                                                                       <  3:990000/149‑1 (MQ=255)
                                             aTGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTc                                                                                                 >  1:723155/1‑149 (MQ=255)
                                               gggATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCAc                                                                                               >  3:1250442/1‑149 (MQ=255)
                                               gggATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCAc                                                                                               <  2:723155/149‑1 (MQ=255)
                                                  aTTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGc                                                                                            >  2:247173/1‑149 (MQ=255)
                                                      tGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGAt                                                                                        >  2:43086/1‑149 (MQ=255)
                                                       gCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATg                                                                                       <  4:655351/149‑1 (MQ=255)
                                                               tGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCtt                                                                                                                      >  4:1027086/1‑110 (MQ=255)
                                                               tGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCtt                                                                                                                      <  3:1027086/110‑1 (MQ=255)
                                                               tGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGttt                                                                               <  4:662082/149‑1 (MQ=255)
                                                                  tACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCAt                                                                            <  2:983776/149‑1 (MQ=255)
                                                                   aCTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATc                                                                                                         <  4:1215608/119‑1 (MQ=255)
                                                                   aCTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATc                                                                                                         >  1:200498/1‑119 (MQ=255)
                                                                   aCTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATc                                                                                                         >  3:1215608/1‑119 (MQ=255)
                                                                    ctctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATgc                                                                          >  3:881380/1‑149 (MQ=255)
                                                                     tctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCtt                                                                                                                      >  1:985023/1‑104 (MQ=255)
                                                                     tctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCaa                                                                                                       <  2:632589/119‑1 (MQ=255)
                                                                     tctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCaa                                                                                                       >  1:632589/1‑119 (MQ=255)
                                                                        tCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATgccgcc                                                                      >  2:240422/1‑149 (MQ=255)
                                                                          gTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCa                                                                    >  1:363949/1‑149 (MQ=255)
                                                                            gggCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATc                                                                  <  1:240422/149‑1 (MQ=255)
                                                                               cGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                               <  1:247173/149‑1 (MQ=255)
                                                                                    gggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                               <  4:1373871/144‑1 (MQ=255)
                                                                                    gggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                               >  3:1373871/1‑144 (MQ=255)
                                                                                    gggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAAc                                                          <  1:43086/149‑1 (MQ=255)
                                                                                       tttACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATc                                                                  <  4:1129524/138‑1 (MQ=255)
                                                                                       tttACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATc                                                                  >  3:1129524/1‑138 (MQ=255)
                                                                                       tttACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCtt                                                       <  2:340294/149‑1 (MQ=255)
                                                                                         tACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCa                                                     <  3:1098696/149‑1 (MQ=255)
                                                                                          aCCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATg                                                                           >  2:684258/1‑126 (MQ=255)
                                                                                          aCCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCAc                                                    <  2:363949/149‑1 (MQ=255)
                                                                                          aCCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCAc                                                    <  1:534048/149‑1 (MQ=255)
                                                                                             tGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGc                                                                                                                                              >  3:82838/1‑56 (MQ=255)
                                                                                             tGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGc                                                                                                                                              <  4:82838/56‑1 (MQ=255)
                                                                                              gCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAAt                                                              >  4:573497/1‑135 (MQ=255)
                                                                                              gCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAAt                                                              <  3:573497/135‑1 (MQ=255)
                                                                                              gCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCa                                                     <  3:970172/144‑1 (MQ=255)
                                                                                              gCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCa                                                     >  4:970172/1‑144 (MQ=255)
                                                                                               cATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTg                                                                                                                                                <  4:869186/52‑1 (MQ=255)
                                                                                               cATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTg                                                                                                                                                >  3:869186/1‑52 (MQ=255)
                                                                                                    cAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTtgt                                          <  4:881380/149‑1 (MQ=255)
                                                                                                     aaaGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATc                                                                  <  1:730996/124‑1 (MQ=255)
                                                                                                     aaaGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATc                                                                  >  2:730996/1‑124 (MQ=255)
                                                                                                     aaaGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATaaa                                                           >  2:356332/1‑131 (MQ=255)
                                                                                                       aGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTACCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCAAATAAACCTTCACTAATACTTGTGCt                                       <  1:1019119/149‑1 (MQ=255)
                                                                                                           tttGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGt                                                                                     <  4:1283912/99‑1 (MQ=255)
                                                                                                                ggATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATg                                                                                       <  1:1071870/92‑1 (MQ=255)
                                                                                                                ggATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATg                                                                                       >  2:1071870/1‑92 (MQ=255)
                                                                                                                ggATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCt                              <  4:795303/149‑1 (MQ=255)
                                                                                                                 gATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATa                                                             <  1:1139982/117‑1 (MQ=255)
                                                                                                                 gATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATa                                                             >  2:1139982/1‑117 (MQ=255)
                                                                                                                  aTCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTc                               <  3:199769/146‑1 (MQ=255)
                                                                                                                  aTCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTc                               >  4:199769/1‑146 (MQ=255)
                                                                                                                  aTCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGc                            >  1:1078808/1‑149 (MQ=255)
                                                                                                                  aTCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGc                            <  3:75319/149‑1 (MQ=255)
                                                                                                                      tGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTTTGTTTCAt                                                                            <  3:1349168/97‑1 (MQ=255)
                                                                                                                      tGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCAt                                                                            >  4:1349168/1‑97 (MQ=255)
                                                                                                                        tttGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTc                      <  2:1078808/149‑1 (MQ=255)
                                                                                                                         ttGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATACCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCg                     >  1:1449905/1‑149 (MQ=255)
                                                                                                                            cTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGAtt                  >  4:327329/1‑149 (MQ=255)
                                                                                                                              ccTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTc                                                      <  4:388744/111‑1 (MQ=255)
                                                                                                                               cTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTaaa               >  4:1138798/1‑149 (MQ=255)
                                                                                                                               cTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTaaa               >  3:1091593/1‑149 (MQ=255)
                                                                                                                                  aCAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCg            >  4:393246/1‑149 (MQ=255)
                                                                                                                                   actcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCATGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTaaa               <  4:872934/142‑1 (MQ=255)
                                                                                                                                   actcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTaaa               >  3:872934/4‑145 (MQ=255)
                                                                                                                                     tcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGc                                        >  1:163091/2‑118 (MQ=255)
                                                                                                                                     tcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGc                                        <  2:163091/117‑1 (MQ=255)
                                                                                                                                     tcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGaa          <  3:77568/147‑1 (MQ=255)
                                                                                                                                     tcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGaa          >  4:77568/2‑148 (MQ=255)
                                                                                                                                       gccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGt       >  4:447966/1‑149 (MQ=255)
                                                                                                                                       gccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                               >  3:1334911/1‑93 (MQ=255)
                                                                                                                                       gccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                               <  4:1334911/93‑1 (MQ=255)
                                                                                                                                        ccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGtt      >  3:829901/1‑149 (MQ=255)
                                                                                                                                         cagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCAc                                                    >  1:1152265/1‑102 (MQ=255)
                                                                                                                                         cagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCAc                                                    <  2:1152265/102‑1 (MQ=255)
                                                                                                                                            cgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCAt  <  3:393246/149‑1 (MQ=255)
                                                                                                                                            cgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCAt  <  3:542421/149‑1 (MQ=255)
                                                                                                                                               aCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTc                      >  2:1393166/1‑126 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCAT  >  NC_000913/4093320‑4093608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: